-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
1/16
Khpa`| Fkg`bankp|v{ mhl Ngda`qdmv Fkgdgj{ 0< "655
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
2/16
bgqpa a| md($ =;;;+ mhl mva gha gi |ba nmcgv `dmppap giahlguau|klmpap$ p|vghjd{ knudk`m|al kh |ba lkjap|kgh giuvg|akhp kh |ba jq| "Muudafmqn$ =;21? \avvm mhl Iav.vakvm$ =;;
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
3/16
m``gvlkhj |g |ba nmhqim`|qvavp khp|vq`|kghp qpkhj pua.`k` uvknavp( \ba 15 VM@A zava uavigvnal zk|b pua.`k` mh|kpahpa uvknavp lavkyal ivgn |ba V\.U@Vpa}qah`ap$ mhl |ba 05 VM@A zk|b pua`k` pahpa uvknavplavkyal ivgn |ba 15 ahl gi |ba 15 VM@A pa}qah`ap( \bamnudkal `LHMp zava `dghal kh|g |ba udmpnkl ya`|gvpu@V6(= "Khyk|vgjah+ gv uJAN.\ Amp{ "Uvgnajm+ mhlva`gnfkhmh| `dghap zava pa}qah`al kh fg|b p|vmhlp khmh mq|gnm|al LHM pa}qah`av( @gnuq|av mhmd{pkp gi|ba LHM mhl mnkhg m`kl pa}qah`ap zmp lgha qpkhj|ba J@J um`omja "Jaha|k`p @gnuq|av Jvgqu$ Kh (+$fkgkhigvnm|k`p vapgqv`ap gi |ba H@FK bgnaumja"b||u3''zzz(h`fk(hdn(hkb(jgy+$ |ba AFK zafpk|a"b||u3''zzz(afk(m`(qo'+ mhl NGLADDAV "Pmdk mhlFdqhladd$ =;;0+( \ba khk|kmd lm|mfmpa pamv`bap$ ivgnzbgpa vapqd|p |ah|m|kya iqh`|kghmd mhhg|m|kghp igv |baumv|kmd pa}qah`ap zava gf|mkhal$ zava `mvvkal gq| qpkhjIMP\M "Uamvpgh$ =;;5+ kh |ba Pzkppuvg| mhl \vanfdlm|mfmpap "Fga`onmhh a| md($ 6550+( M lahlvgjvmn zmp
`md`qdm|al qpkhj |ba uvgjvmnp UVG\LKP\ mhlHAKJBFGV gi |ba Ub{dku um`omja "Iadpahp|akh$ =;2;+(Mhmd{pkp gi pkjhmd uau|kla pa}qah`ap$ kh`dqlkhj `damymjapk|a dg`mdksm|kgh$ zmp nmla qpkhj PkjhmdU pgi|zmva"Hkadpah a| md($ =;;:+ m| pavyavp gi |ba @ah|av igv Fkg.dgjk md Pa}qah`a Mhmd{pkp$ Fkg@ah|vqn.L\Q$ \a`bhk.`md Qhkyavpk|{ gi Lahnmvo "b||u3''zzz(`fp(l|q(lo'pavyk`ap'PkjhmdU'+(
6(1( Pgq|bavh fdg|p
M( jvmhlkp jahgnk` LHM zmp kpgdm|al m``gvlkhj |g
Pmnfvggo a| md( "=;2;+( LHM lkjap|p "=1 dj uav dmha+zava paumvm|al gh m 5(2! mjmvgpa jad mhl |vmhpiavval|g B{fghl.H/ "Mnavpbmn+ h{dgh nanfvmhap qpkhjp|mhlmvl uvg`alqvap "Pmnfvggo a| md($ =;2;+( \baah|kva `LHMp zava dmfadal zk|b _m.06U^l@\U |g m bkjbpua`k` m`|kyk|{ qpkhj |ba Valkuvkna LHM.dmfadkhj ok|"Mnavpbmn+ mhl qpal |g uvgfa |ba fdg|p( \ba d|avpzava zmpbal zk|b 5(= PP@ m| 91
y
@(
6(9( Hgv|bavh fdg|p
\g|md VHM ivgn M( jvmhlkp kh ymvkgqp layadgunah.|md p|mjap "haghm|a dmvyma$ |bkvl.khp|mv dmvyma$ nkljq|p
ivgn |bkvl.khp|mv dmvyma$ uquma$ hgh.iaalkhj mlqd|p$ =5.lm{.gdl mlqd|p$ mhl nkljq|p ivgn =5.lm{.gdl mlqd|p+zmp uvaumval qpkhj |ba VHamp{ VHM ar|vm |kgh ok|"]kmjah+( Jad ada`|vgubgvapkp "Igqvha{ a| md($ =;22+ "2dj uav dmha+ mhl b{fvklksm|kghp "Pmnfvggo a| md($ =;2;+zava lgha m``gvlkhj |g p|mhlmvl uvg`alqvap( \ba d.|avp zava zmpbal zk|b 5(= PP@ m| 91
y
@(
6(:( Vayavpa hgv|bavh
M =55 hj mdk}qg| gi m pada`|al `dgha ivgn am`b gi |ba=< `LHM pa}qah`ap gi |ba U@V ivmjnah|p zmp lg|
fdg||al kh|g h{dgh nanfvmhap "B{fghl.H/+ mhl
b{fvklksal |g dmfadal |g|md `LHM uvgfap uvaumval
ivgn M( jvmhlkp |bkvl.khp|mv zbgda dmvyma nkljq|p$ mhl
zbgda dmvyma iaal zk|b |ba pavkha uvg|akhmpa khbkfk|gvp
PO\K gv F\@K( \g|md VHM zmp uvaumval qpkhj |ba
VHamp{ VHM ar|vm |kgh ok| "]kmjah+( \ba `LHM
uvgfap zava p{h|bapksal f{ vayavpa |vmhp`vku|kgh gi 1dj gi |g|md VHM qpkhj NNDY.V\ "Khyk|vgjah+$ 15
d@k _m.06U^l@\U mhl 5(1 dj gi gdkjg.l"\+=9 uvknav(
\zg vaudk`m|ap gi |ba b{fvklksm|kghp zava lgha? mfgq|
=6 =0 =59 `un gi am`b dmfadal `LHM zmp qpal kh
|ba vp| vaudk`m|a mhl 2 =5 =59 `un kh |ba pa`ghl(
\ba hgvnmdksm|kgh gi |ba mngqh| gi LHM dgmlal kh
|ba fdg|p zmp lgha f{ b{fvklkskhj m vaudk`m|a d|av zk|b
|ba dmfadal fm`ofgha gi |ba ya`|gvp `mvv{khj |ba `LHM
khpav|p$ mhl qpkhj |bapa lm|m |g mlcqp| |ba ymdqap ivgn
|ba b{fvklksm|kghp zk|b |ba dmfadal `LHMp( \ba
uJAN.\ Amp{ "Uvgnajm+ gv u@V6(= "Khyk|vgjah+ ya`.
|gvp zava dmfadal zk|b _m.06U^l@\U qpkhj |ba Valk.
uvkna LHM.dmfadkhj ok| "Mnavpbmn+( Ikd|avp zava
zmpbal zk|b 5(= PP@ m| 95y
@( \ba b{fvklksal mhl
zmpbal nanfvmhap zava arugpal |g m Fkgnmr dn
"Oglmo+ mhl |ba knmja p`mhhkhj zmp uavigvnal zk|b m
|vmhpumvah`{ p`mhhav "Lqg.p`mh MJ.=655 MJIM+(
\ba pmnuda ukrad kh|ahpk|{ zmp nampqval zk|b Savg.
Lp`mh uvgjvmn "P`mhmd{|k`p+( \ba lahpk|{ lm|m zava
mhmd{sal qpkhj m nglad `ghpklavkhj |ba igddgzkhj pgqv.
`ap gi ymvkm|kghp3 |ba `LHM uvgfap ivgn M( jvmhlkp$
|ba pavkha uvg|akhmpap jahap$ |ba kh|avm`|kgh fa|zaah
|bapa im`|gvp$ vaua|k|kghp "|zg+ mhl aruavknah|md avvgv(Vaua|k|kgh mhl aruavknah|md avvgv zava |vam|al mp vmh.
lgn mhl |ba g|bavp mp ral asta`|p( \ba nglad mdpg
kh`dqlal mp `gymvkm|a |ba lahpk|{ gfpavyal zk|bkh |ba
hgvnmdksm|kgh `gh|vgd aruavknah|( \ba nglad zmp mhm.
d{sal qpkhj |ba UVG@ NKRAL gi PMP "6555+(
0( Vapqd|p
0(=( Uvg|agd{|k` m`|kyk|{ kh |ba jq| gi M( jvmhlkp
Uvaykgqp vapqd|p "Uqv`add a| md($ =;;6? Ivmh`g a| md($
655
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
4/16
0(6( @dghkhj mhl `bmvm`|avksm|kgh gi m pavkha uvg|akhmpanqd|kjaha imnkd{ ivgn M( jvmhlkp
\ba klah|k`m|kgh gi pavkha uvg|akhmpa m`|kyk|{ kh fgddzaaykd uvgnu|al qp |g klah|ki{ mhl kpgdm|a jahap ah`gl.khj |bkp m |kyk|{( Igv |bm| za qpal m jahavk U@Vmuuvgm`b qpkhj lajahavm|a gdkjghq`dag|kla uvknavpfmpal gh `ghpavyal pajnah|p igqhl kh pavkha uvg|ak.hmpap( M pknkdmv muuvgm`b igv |ba kpgdm|kgh gi pavkha
uvg|akhmpap bmp faah pq``appiqdd{ muudkal |g g|bavmv|bvguglp "Nqddav a| md($ =;;0? Adykh a| md($ =;;0$ =;;
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
5/16
pa}qah`ap zava lkpvajmvlal kh mdd pqfpa}qah| mhmd{pap(
\ba gyavmdd klah|k|{ fa|zaah |ba mnkhg m`kl pa}qah`ap
lavkyal ivgn |ba U@V ivmjnah|p kp vanmvomfd{ dgz
zk|b m namh umkvzkpa klah|k|{ gi 66(=! mhl cqp| pkr
Ikj( 6( Pa}qah`a mdkjhnah| gi |ba `gnuda|a mhl U@V ivmjnah|p "u+ pavkha uvg|akhmpa pa}qah`ap gf|mkhal zk|b |ba nglad ahs{na fgykha `b{ng.|v{upkh( Hqnfavkhj gi fgykha `b{ng|v{upkh kp pbgzh mfgya |ba mdkjhnah|( \ba `m|md{|k` |vkml kp nmvoal zk|b mvvgzbamlp$ |ba vajkghp gi |bapa}qah`ap gi |ba U@V ivmjnah|p `gvvapughlkhj |g |ba uvknavp qpal kh U@V mva pbmlal$ mhl klah|k`md ugpk|kghp anfgdlahal mhl k|mdk`ksal(
G(F( Gdkyakvm.Ha|g a| md( ' Khpa`| Fkg`bankp|v{ mhl Ngda`qdmv Fkgdgj{ 0< "655
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
6/16
mnkhg m`kl vapklqap mva `ghpavyal kh mdd pa}qah`ap(
Khymvkmh| vapklqap$ pbgzh kh fgdl kh Ikj( 6$ `gnuvkpa
|ba Mpu=56$ mdvaml{ lkp qppal$ mhl `{p|akha vapklqap$
mdghj zk|b |zg jd{`kha vapklqap uvapqnmfd{ `ghpavyal
igv p|vq`|qvmd vampghp(@gnumvkpghp gi |ba lalq`al mnkhg m`kl pa}qah`ap
zk|b |ba Pzkppuvg| \vanfd lm|mfmpa zava `mvvkal gq|(
\ba lm|mfmpa pa}qah`a ngp| pknkdmv |g am`b M( jvmhlkp
pa}qah`a gi |ba U@V ivmjnah|p kp pbgzh kh \mfda =(
\bapa pa}qah`a `gnumvkpghp vayamd umkvzkpa klah|k|kap
kh |ba vmhja 0
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
7/16
|ban( M pa`ghl jvgqu `gnuvkpap Mjpav2u$ Mjpav=6umhl Mjpav6=u$ zbk`b pbmva 6:0:! umkvzkpa mnkhgm`kl pa}qah`a klah|k|{ mhl 05 klah|k`md vapklqap mnghj
|ba |bvaa pa}qah`ap( \ba |bvaa pa}qah`ap mva mdd pknkdmv|g |v{upkhp( \ba bkjbap| uav`ah|mja klah|k|{ zk|b mlm|mfmpa pa}qah`a gi mdd mnudkal `dghap zmp gf|mkhaligv Mjpav=6u$ 1;! klah|k`md m| |ba mnkhg m`kl pa}qah`a|g m |v{upkh uva`qvpgv ivgn Lkmuvauap mffvaykm|qp$ |bapqjmv`mha vgg|p|mdo fgvav zaaykd(
M |bkvl jvgqu `gh|mkhp Mjpav=:u mhl Mjpav
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
8/16
0(0( @dghkhj gi |bvaa |v{upkh jahap ivgn M( jvmhlkp mhlmhmd{pkp gi |ba lalq al uvg|akh pa}qah ap
\g gf|mkh |ba `gnuda|a pa}qah`a gi |ba |v{upkhah`glkhj `LHMp Mjpav6u$ Mjpav9u mhl Mjpav6=u$ zavp| uavigvnal 15 VM@A qpkhj |bvaa mh|kpahpa uvknavp
fmpal gh |ba V\.U@V pa}qah`ap$ igddgzal f{ m 05
VM@A qpkhj |zg gv |bvaa pahpa uvknavp dg`m|al m| |ba
15 ahl gi |ba pa}qah`ap lavkyal ivgn |ba 15 VM@A( \ba
`gnuda|a `LHM pa}qah`ap mhl |ba lalq`al uvg|akh
pa}qah`ap mva pbgzh kh Ikj(
-
8/9/2019 Diverse Family of Serine Proteinase Genes Expressed in Cotton Boll Weevil (Anthonomus Grandis) Implications for t
9/16
Mjpav6 mhl Mjpav6= mva p|vq`|qvmdd{ yav{ pknkdmv( \baMjpav6 uvalk`|al uvg|akh pa}qah`a kh`dqlal m =9 mnkhgm`kl pkjhmd uau|kla igddgzal f{ m pbgv| uvglgnmkh 6
