Differential Gene Expression in Heterosigma akashiwo in Response to Model Flue Gas: Where Does the Carbon Go? 8 th Annual Algae Biomass Summit Algal Strain Development October 2, 2014 Jennifer J. Stewart, Ph.D. Scientist, NSF SEES Fellow 302-645-4371 [email protected]R83-3221
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Differential Gene Expression in Heterosigma akashiwo in Response to Model Flue Gas: Where Does the Carbon Go? 8 th Annual Algae Biomass Summit Algal Strain.
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Differential Gene Expression in Heterosigma akashiwo in Response to
Model Flue Gas:Where Does the Carbon Go?
8th Annual Algae Biomass SummitAlgal Strain Development
October 2, 2014
Jennifer J. Stewart, Ph.D.Scientist, NSF SEES Fellow
1641 Transcripts Were Significantly Differentially Expressed
524 Received KEGG Orthology (KO) Identifiers
274 Received Enzyme Commission (EC) Numbers
149 Mapped to KEGG Reference Pathways
Global Gene Expression Analysis
7
Metabolic Overview of Differentially Expressed Genes
8
Photosynthesis
Carbohydrate
Carbohydrate
TCA
Lipid Syn
N-Metabolism
AA
Isoprenoids
Abiotic Stress
Redox
Nucleotides
RNA Processing
DNA Synthesis
Protein SynthesisSignaling
Cell Cycle
Transporters
Unknowns
Metabolic Overview of Differentially Expressed Genes
9
For NR, Only NR2 sequences were Found to be Differentially Expressed The Highest Up-regulated Transporter Genes were Phosphate Transporters Flue Gas is a Significant Source of Nitrogen for Protein Synthesis
Nitrogen Uptake and Utilization
10
C14:0
C15:0
C15:1
C16:0
C16:1
C16:1
C17:0
C17:1
C18:0
C18:1
C18:2n
6
C18:3n
3C18
:4
C20:1n
9
C20:5n
3
C22:1n
9C22
:2
C22:6n
30
2
4
6
8
10
12
14
16
18Air Flue Gas
FA
ME
(p
g/ce
ll)Fatty Acid Biosynthesis
Increase in FAs involved in plastid membrane composition
11
Fatty Acid Biosynthesis
12
Carbonate Chemistry
13
CO2 Fixation During Growth on Model Flue Gas
a-D-Glucose-6P
b-D-Glucose-6P b-D-Fructose-6P
b-D-Fructose-1,6P2
Glyceraldehyde-3PGlyceraone-P
Glycerate-1,3P2
Glycerate-3P
Glycerate-2P
Phosphoenolpyruvate Pyruvate
Carbon Fixation
AA Synthesis Fatty Acid Synthesis
Up-Regulation Seen Throughout the
Glycolysis Pathway
_x0003_Air _x0008_Flue Gas0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Carbohydrate LipidProtein
Heterosig
ma aka
shiw
o
Nannoch
loropsis
sp.
0%
20%
40%
60%
80%
100%
Carbohydrate LipidProtein
Pathway for Storage Carbohydrates?12%CO2 + 150 ppm NO 2%CO2 – Day 5 Batch Growth
Pathway for Storage Carbohydrates?
16
Green algae = StarchDiatoms = Chrysolaminarin
Jennifer J. Stewart, Ph.D.Scientist, NSF SEES Fellow