Development of SCAR and CAPS Markers Linked to the Beta Gene in Tomato.

Development of SCAR and CAPS Markers Linked to the Beta Gene in Tomato

草地研究室

指導老師：王裕文 老師報告學生：楊雯惠報告時間： 3月 21 日上午 10 點 35 分報告地點：農藝館 112

作者： Yiping Zhang and John R. Stommel

出處： Crop Science 41 (2001) 1602–1608

草地研究室

β - carotene

Phenotypic selection

Marker-assisted selection

• Provitamin A carotenoid

• Essential nutrient

• Antioxidant

• Reduced risk of heart disease and certain cancers

Beta gene

草地研究室

Marker-assisted selectionRAPD

• Simple

• Rapid

• Inexpensive

• Radioactive isotopes

• Analyzing large numbers of samples

草地研究室

1100bp

830bp

High Low

TAG(2000) 100:368-375

MPOPRBOBR

OPAR18

UBC792

草地研究室

Marker-assisted selectionRAPD

• Poor reproducibility

• Multiple amplification products

• Dominant marker

草地研究室

sequence characterized amplified region,

SCAR

草地研究室

草地研究室

SCBC792682

Mismatch

草地研究室

How to solve ?

Modification of PCR parameter

Restriction enzyme digestion

Primer redesign

• increasing annealing temperature

• increasing Mg++ concentrations

草地研究室

Cleaved amplified polymorphic sequence,

CAPS

草地研究室

bb

BB

bb

BB

bb

BB

草地研究室

HinfⅠ

RsaⅠ

SCAR18

SCBC792

草地研究室

草地研究室

Codominant Marker

Dominant Marker

草地研究室

Discussion

Specific

Stable

Reproduced

Dominant or codominant Marker

SCAR

Advantage of RAPD

草地研究室

謝謝大家！