Development of a duplex real-time PCR assay for the differentiation of Blastomyces dermatitidis and B. gilchristii and a retrospective study of blastomycosis in New York (2005-2019)

1 Development of a duplex real-time PCR assay for the differentiation of Blastomyces dermatitidis and B. gilchristii and a retrospective study of blastomycosis in New York (2005-2019) Mitchell Kaplan 1, # , YanChun Zhu 1,# , Julianne V Kus 2, 3 , Lisa McTaggart 2 , Vishnu Chaturvedi 1,4 , and Sudha Chaturvedi 1,4* 1 Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12208 2 Public Health Ontario, Canada 3 Dept. of Laboratory Medicine and Pathobiology, University of Toronto, Canada 4 Dept. of Biomedical Sciences, University at Albany, Albany, NY Corresponding author: Sudha Chaturvedi; email: [email protected]; phone: 518-474-7563 Running title: Duplex real-time PCR assay for Blastomyces # Mitchell Kaplan and YanChun Zhu contributed equally All rights reserved. No reuse allowed without permission. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 14, 2020. ; https://doi.org/10.1101/2020.08.11.20172932 doi: medRxiv preprint NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice.

Development of a duplex real-time PCR assay for the differentiation of Blastomyces dermatitidis and B. gilchristii and a retrospective study of blastomycosis in New York (2005-2019)

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