1 DEPARTMENT OF ZOOLOGY Bacteriological testing of Drinking water OBJECTIVES The present study was undertaken to, • determine the presence of faecal coliforms in water samples by presumptive test • confirm the faecal coli forms by EMB agar and Gram staining method • determine presence of faecal Streptococci by KFC test. • determine the MPN index of faecal coliforms and faecal Streptococci METHODOLOGY & TECHNICAL ASPECT The study was conducted on water sources to assess the extent of bacterial contamination from December 2011 to February 2012. Four different water samples were collected such as Drinking water, Ground water, House hold water, Main tank water. The water samples were subjected to faecal coliform and streptococcal analysis. Total coliforms were detected in multiple-tube technique. In this method, coliforms are detected in two stages such as presumptive test and confirmatory test. Most probable number in the water samples were analysed. OUTCOME • Majority of the water sources especially drinking water were found to contain unacceptable level of faecal coliforms and faecal Streptococci • All the water samples collected during the month of December were seemed to have high MPN index/100 ml, whereas the drinking water samples collected during months of December, January, February 2012 seemed to have high MPN index/100 ml
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1
DEPARTMENT OF ZOOLOGY
Bacteriological testing of Drinking water
OBJECTIVES
The present study was undertaken to,
• determine the presence of faecal coliforms in water samples by presumptive test
• confirm the faecal coli forms by EMB agar and Gram staining method
• determine presence of faecal Streptococci by KFC test.
• determine the MPN index of faecal coliforms and faecal Streptococci
METHODOLOGY & TECHNICAL ASPECT
The study was conducted on water sources to assess the extent of bacterial
contamination from December 2011 to February 2012. Four different water samples
were collected such as Drinking water, Ground water, House hold water, Main tank
water. The water samples were subjected to faecal coliform and streptococcal analysis.
Total coliforms were detected in multiple-tube technique. In this method, coliforms are
detected in two stages such as presumptive test and confirmatory test. Most probable
number in the water samples were analysed.
OUTCOME
• Majority of the water sources especially drinking water were found to contain
unacceptable level of faecal coliforms and faecal Streptococci
• All the water samples collected during the month of December were seemed to
have high MPN index/100 ml, whereas the drinking water samples collected
during months of December, January, February 2012 seemed to have high MPN
index/100 ml
PLANNING & IMPLEMENTATION
• Regular disinfection of water sources and periodic bacteriological appraisal of
drinking water sources
• Awareness can be created among the people of Thondaiman
effects of drinking contaminated water and transmission of water borne diseases.
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PLANNING & IMPLEMENTATION
Regular disinfection of water sources and periodic bacteriological appraisal of
Awareness can be created among the people of Thondaimanpatti about the
effects of drinking contaminated water and transmission of water borne diseases.
Regular disinfection of water sources and periodic bacteriological appraisal of
patti about the
effects of drinking contaminated water and transmission of water borne diseases.
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