2 0 4 0 6 0 8 0 1 0 0 M t P U B 1 : - - - - - - - - - - - - - M N D P R S K M M I S P G L L P T E S L L D S L I L I S N E V S S M Q K F P - - L V Q I K N V S S M I R R I K L L S S L F E E I Q E S D S P L P P S S I L C F I E I F S V I T : 8 5 L j S G A 0 1 9 0 2 : - - - - - - - - - - - - - M N D A V S A M M I S S G L L P T G T L L D S L I H I S N E V G S M E K L P - - L V H T R N V S S M I R R I K L L S S L F E E I Q E T D S P L P P S S I L C F T E L Y S V I T : 8 5 G m 0 2 g 4 3 1 9 0 : - - - - - - - - - - - - - - - - - - - - - M V S P G L L P S G T L L D S L I H V C N E V G S M E K F P - - L V H T R N V S T M I R R I K L L S S L F E E I Q E T D T P L P P S S I L C L T E L F S V I R : 7 7 L j B 0 2 K 2 0 : - - - - - - - - - - - - - - - - - - - - M M I S S T F L P N K S L L D S L I N I S N E A S S M E K F P - - F L H A R N V T S M I R R I K V L S S L F E E I Q V S D T P L P P S S I L C F T E F F S L I T : 7 8 P t 0 8 s 0 7 1 6 0 : - - - - - - - - - - - - - - - - - - - - - - - - - - - M P T G S L M K S L I H I S N E V A S V E K L P - - F S Q F R N I S T M I R R I K F L S F L F E D I R E T N S P L P P S S L L C L T E L F S V I Q : 7 1 P t 1 0 s 1 9 3 3 0 : - - - - - - - - - - - - - M N V A L P P I M I S S V F L P T G S L V E S L I H I S N E V A S V E K L P - - F V Q F R N I S T M I R R I K L L S S L F E D I R E T N S P L P P S S I L C L T E L F S V I Q : 8 5 V v 2 9 3 3 3 0 0 1 : - - - - - - - - - - - - - M D V A L P T L M V S S G F L P T G S L L E S L I H I S N E V A S M E R L P - - V I Q V R N I S T M I R R I K L L S S L F E E I H E M N T P L P P S S I L C L T E L F S V I R : 8 5 B n A R C 1 : M A T D S A M F A S S R R R Q S P S L E A F L S P V D L S D V P L L Q T L S S I S S E I V S C F S N A R F S F Q R R N T R S L I R K V Q V F A V L L Q H L A P - E S S L D P T A V L C F K E L Y L L L H : 9 9 1 2 0 1 4 0 1 6 0 1 8 0 2 0 0 M t P U B 1 : R V K V L I Q E C T D G S S L W S L I Q L D F I S N Q F F V L V K E M G R A L D I L P - L N L L N V A Q D I K E Q V D L L H K Q S K R V E L E L F I D P R E V Q R R E N L F E V M S K N C L Q N K K T N : 1 8 4 L j S G A 0 1 9 0 2 : R V K D L I Q E C K D G S T L W G L I Q L E L I S N Q F Y V L A K E L G R A L D I L P - L K L L N I T A D T K E Q V Q L L Q K Q A K R - - V E L L I D P R E L Q R R E Q L L Q V M A N N S L R N K K - - : 1 8 0 G m 0 2 g 4 3 1 9 0 : R V K V L I Q D C K D G S S L W S L I Q L E F I S N Q F Y V L V K E M G R A L D I L S - L S L L N V T S D I K E Q V E L L H K Q A K R - - A E L L I D P R E L H R R E Q L I Q - - - - - - - - - K K - - : 1 6 3 L j B 0 2 K 2 0 : K V K L L I Q E C K D G S N L W G L I Q L E F I S N Q F Y V L A K E M G R V L D I L P - V S L L N I T T D M R E Q L E I L H K Q T K K - - I E L F I D P R D L Q R R D Q L L Q V M A N K S L P H N K - - : 1 7 3 P t 0 8 s 0 7 1 6 0 : R V K L L I Q R C K G G S S L W G L I Q T E F L S N Q F H A L V K E M G G A L D I L P - L N L L N L S T D T R E Q V E L L H K Q A K R - - V D L L V D P Q E L Q R R E E L L Q I M A S N N Q K N S R - - : 1 6 6 P t 1 0 s 1 9 3 3 0 : R V K L L I Q G C K D G S S L W G L I Q T Q F L S N Q F H V L V K E M G G A L D I L P - L S L L N L S A D T R E Q V E L L H R Q A K R - - F D L L V D P R E L Q R R E E L L Q I M T R N N Q K N S G - - : 1 8 0 V v 2 9 3 3 3 0 0 1 : R V K S F I Q G C K D A S S L W S L V Q T E L I S S Q F H V V V K E M G R A L D I L P - L S L L N I T A D I R E Q V E L L H K Q A K R - - V D L F V D P R E L Q R R E E L L Q V M A T N N E K N A K - - : 1 8 0 B n A R C 1 : H S K F L L R Y C A H S S K L W L L L Q S P S L S S F F H D L S K D Y S T L L D V F L P V D S L C L N D D V R E Q V Q L L H M Q H Y V - - - D D N N D E T L R N R L Y S F L D E F E N G S V P N S E - - : 1 9 4 2 2 0 2 4 0 2 6 0 2 8 0 3 0 0 M t P U B 1 : N N K G F I D F V K V E E I M C S I G L R T L S D Y V E E I S K L E V E A Q N Q A G T G G L I V V S N I N N L M S L V S Y T K S M V F R N - - - D G E S E E C K P I S M F L Y N K S K I H D N D S S S S : 2 8 1 L j S G A 0 1 9 0 2 : - N K G F I D F G K V E D I L S S I G L R T P S D Y D G E I S N L E V E A Q N Q A G T G G L I V V S N I N N L I S L V S Y S K S M I F R N G E N D R D E E E C K P L S L I L Y N K N - - H D - - - S S S : 2 7 4 G m 0 2 g 4 3 1 9 0 : - - K G L V D F G K V E E I L S S I G L R T P S D Y E E E I S K L E A E A Q N Q A G T G G L I V V S N I N N L I S L M C Y S K S M I F K E G E S D T K E D - - - - - - - - L Y D S S - - - - - - - S S S : 2 4 6 L j B 0 2 K 2 0 : - S K G F I D L R K L E E I L S S I G F R S P S D Y D R E I S K L E A E A Q N Q A G T G G L M V V S N I N N L L C V V S H A K S M F F E D G E S K K D E R N C K K L S E F L Y V K I H D S S - - - S S S : 2 6 9 P t 0 8 s 0 7 1 6 0 : - N K G F V D F V K V K E V F S C I G L R S P L D Y E E E I S K L E A E A E K Q A G T G G L I V V S N I N N L I S L V T Y S K S V I F I D R H E E E I K E N F K Q L S A S M N R N Q D V S - - - - S S S : 2 6 1 P t 1 0 s 1 9 3 3 0 : - N K G F D D F V K L K E V L S C I G L R S P L E Y E E E I S K L E A E A E K Q A G T G G L I V V S N I N N I I S L V T S S K S V I F V D R D K E E I K E N F K Q R S A F M N R N Q D V S - - - - S S S : 2 7 5 V v 2 9 3 3 3 0 0 1 : - N K A L V D F R E V K E I L S S I G L R S P L D Y D E E I S K L E A E A Q K Q A G T G G L I V V S N I N N L I S L V L Y S K S M I F S E E H N E K T D E A F K K R S A S F R M N N D H S - - - - S S S : 2 7 5 B n A R C 1 : - - - - - - - - E L R F F F F E K L A I K D P T S Y R E E I E F L E E Q I K S H G - C D L D P T R S V I N G F I D I T R Y V M F L L F K I E D G N E I K K Q R K R L I S E E I E N T F T T - - - - - - - : 2 7 8 3 2 0 3 4 0 3 6 0 3 8 0 4 0 0 M t P U B 1 : S S F S Q S M M T V N I P D E F R C P I S L D L M R D P V I V S S G H T Y D R I S I A E W I N - S G H H T C P K S G Q R L I H T A L I P N Y A L K S L V H Q W C Y E N N V K M N E A I T - - - - K N N N : 3 7 6 L j S G A 0 1 9 0 2 : S S M S I S V I P - N I P D E F R C P I S L D L M R D P V I V S S G H T Y D R N S I A Q W I N - T G H H T C P K S G Q R L I H T A L I P N Y A L K S L V Q Q W C Y E N N V P V N E P M N S - - - L L N N : 3 6 9 G m 0 2 g 4 3 1 9 0 : Q S M T P - - - - - N V P D E F R C P I S L D L M R D P V I V S S G H S Y D R I S I A Q W I N - S G H H T C P K S G Q R L I H T A L I P N Y A L K S L V Q Q W C H D N N V P V D E P T T E - - - G N K N : 3 3 7 L j B 0 2 K 2 0 : Q S M T V - - - - - D I P D E F R C S I S L Q L M R D P V I L S S G H T Y D R N S I A K W I N - S G H H T C P K S G Q R L I H T A L I P N F A L K S L I Q Q W C Q E N K V P V N D P T S L - - - L S E G : 3 6 0 P t 0 8 s 0 7 1 6 0 : Q S I L P - - - - - N I P D E F R C P I S L D L M K D P V I V A S G H T Y D R N S I A Q W I N - S G H H T C P K S G K R L I H T S L I P N Y A L K S L V H Q W C Q D N N V P L I E N S T S S S S K F E R : 3 5 5 P t 1 0 s 1 9 3 3 0 : Q S I L S - - - - - N I P D E F R C P I S L D L M K D P V I V A S G H T Y D R N S I A Q W I N - S G H Q T C P K S G Q R L I H M A L I P N Y A L K S M V H Q W C Q D N N V P L I D D S S S S F S K S E S : 3 6 9 V v 2 9 3 3 3 0 0 1 : Q S A I L - - - - - N I P D E F R C P I S L D L M R D P V I V A S G H T Y D R N S I A Q W I N - T G H N T C P K S G M K L I H M A L I P N Y A L K S L V H Q W C R E N N I Q L I E S T S S S S S D L G R : 3 6 9 B n A R C 1 : - - - - - - - - - - T L P K D F I C S I S L N L M N D P V I I S T G Q T Y D R T S I A R W I H Q E G R S T C P K T G Q K L V D L S F V S N L A L R H L T T L W C E V T G L S H D S P K E S - - - - - - - : 3 6 1 4 2 0 4 4 0 4 6 0 4 8 0 5 0 0 M t P U B 1 : S S S K R H K N E N A I D H I S E N K A S K D A V K M T A E F L V G K L A T G S T D I Q R Q S A Y E I R L L A K T G M D N R R I I A E V G A I P F L V T L L V S K D S R I Q E H V V T A L F N L S I Y D : 4 7 6 L j S G A 0 1 9 0 2 : S R S K K N L K E E A I D H I S A N K A A S D A V K M T A E F L V G K L A T G S V D I Q R Q A A Y E L R L L A K S G T N N R S I I P E A G A I P F L V T L L A S Q D S R T Q E H A V T A L F N L S I L D : 4 6 9 G m 0 2 g 4 3 1 9 0 : S S K K - - L K E D A V D H I S A N K A A A D A V K M T A E F L V G K L A T G S A D I Q R Q A A Y E L R L L T K T G M V N R S V I A E V G A I P F L V T L L G S Q D S R I Q E H A V T A L F N L S I F D : 4 3 5 L j B 0 2 K 2 0 : N N S K K N L N E N A V D Q V S T N Q G S T D S V K M T A E F L V G K L A T G S A D I Q M K A A Y E L R L L A K S G M N N R R I I A E A G A I P F L V T L L G S N D S R I Q E N A V T A L L N L S I F D : 4 6 0 P t 0 8 s 0 7 1 6 0 : S S S K S K L S E K A I E H I S A T K A A M D A V K M T A E F L V G K L A M G S P E I Q R Q A A Y E L R L L A K T G M D N R K I I A E A G A I P F L V T L L S S T D P R I Q E N A V T A L L N L S I F D : 4 5 5 P t 1 0 s 1 9 3 3 0 : S S G K S K L S E K A I D H I S A T K A A M D A V K M T A E F L V G K I A M G S P E I Q R Q A T Y E L R L L A K T G M G N R R I I A E A G A I P F L V T L L S S T D P K T Q E N A V T A M L N L S I L E : 4 6 9 V v 2 9 3 3 3 0 0 1 : S N S M R K S C E K A V D H I S A T K T A M D A V K M T A E F L V G K L A T G S P E I Q R Q A A Y E L R L L A K T G M D N R R I I A E A G A I P F L V T L L S S H D P R I Q E N A V T A L L N L S I F D : 4 6 9 B n A R C 1 : - - - - - - - - - - - L P K V F Q T R A S T E A N K A T L S I L V Q N L A H G S - - - - E L A A G E I R V L T R T V T E T R T L I V E T G A I P Y L R S L L K S Q N A V A Q E N A V A S I F N L S I D E : 4 4 6 5 2 0 5 4 0 5 6 0 5 8 0 6 0 0 M t P U B 1 : N N K I L I M A A G - A I D N I V E V L E F G K T M E A R E N A A A A I Y S L S M I D D C K V Q I G A S S R A I P A L V G L L K E G T I I G K R D A A T A L F N L A V Y N P N K L S I V K S G A V T L L : 5 7 5 L j S G A 0 1 9 0 2 : N N K I L I M V A G - A V D N I V E V L E S G K T M E A R E N A A A A I Y S L S M V D D C K V Q I G A C T R A I P A L V A L L K E G T P I G K R D A A S A I F N L A V Y N P N K S S I V K A G A V P L L : 5 6 8 G m 0 2 g 4 3 1 9 0 : N N K I L I M A A G - A V D S I V E V L E S G K T M E A R E N A A A S I Y S L S M V D E C K V Q I G G R P R A I P A L V E L L K E G T P I G K R D A A S A L F N L A V Y N P N K V S V V K A E A V P V L : 5 3 4 L j B 0 2 K 2 0 : N T K I L I M A A G - A V E N I I G V L E S G K S M E A R E N A A A A I F S L S M V D E C K V T I G T H P R A I P A L I G L L K E G T A T G K R D A A T A L F N L A V Y K H N K A S I V H A G A V T L L : 5 5 9 P t 0 8 s 0 7 1 6 0 : N N K I L I M A A G - S I D S I I N V L E S G K T M E A R E N A A A T I F S L S I I S D C K V T I G T R P R A F S A L V G L L R E G T A T G K K D A A S A L F N L S V Y N A N K A S V V V A G A V P L L : 5 5 4 P t 1 0 s 1 9 3 3 0 : N N K T L I M S A G - S I D S I I D V L E S G K T M E A R E N A A A T I F S L S I I N D C K V T I G T R P R A F S A L V G L L R E G T S A G K K D A A S A L F N L S V Y E A N K A S V V V A G A V P L L : 5 6 8 V v 2 9 3 3 3 0 0 1 : N N K I L I M A A G - A I D N I V D V L Q S G K T M E A R E N A A A A I F S L S M I D D C K V T I G A H P R A M P A L V A L L R E G T S A G K R D A A T A L F N L V V Y S A N K G S A V V A G A V P L L : 5 6 8 B n A R C 1 : A N R S L I V E E H D C L E P I M S V L V S G L T M R A K E I A A A T L Y T L S S V H D Y K K A I A N A D G C I E A L A L V L R N G T V R G K K D A V Y A L H S L W L H P D N C S L M V K R G G V S A L : 5 4 6 6 2 0 6 4 0 6 6 0 6 8 0 7 0 0 M t P U B 1 : V E L L M D D K A G I T D D S L A V L A V L L G C S E G L E E I K N S K S L V P L L I D L L R F G S V K G K E N S I T L L L G L C K E E G E L V A M R L L A N P R S I P S L Q S L A A D G S L R A R R K : 6 7 5 L j S G A 0 1 9 0 2 : V E L L M D D K A G I T D D V L A V L A V L Q G C S E G L E E I R - - - - - - - E I I K A F G W P - - - - - - - - - T F L F G F F F K N - - - - - - - - - - - - - - - - - - - - - - L G G Q L K R G K - : 6 2 9 G m 0 2 g 4 3 1 9 0 : V E L L M D D K A G I T D D A L A V L A L L L G C S E G L E E I R N S R A L V P L L I D L L R F G S V K G K E N S I T L L L G L C K Q E G E V V A R R L L A N P R S I P S L Q S L A A D G S L R A R R K : 6 3 4 L j B 0 2 K 2 0 : I E L L M D D K A G I T D D A L A V L A L L L G C S Q G L E E I R N S K E L V P L L V D L L R I G S A R G K E N S I T L L L G L C K E E G E L V A S R L L A N P R S V P S L Q S L A A V G S L R A Q R K : 6 5 9 P t 0 8 s 0 7 1 6 0 : I E L L M D D K A G I T D D A L A L L A L L S G C S E G L E E I R Q S R I L M P M V I D L L R F G S T K G K E N S I T L L L G L C K D G G E E V A R R L L I N P R S I P S L Q S L S T D G S L K A R R K : 6 5 4 P t 1 0 s 1 9 3 3 0 : V E M L M D D K A G I T D D A L A L L A L L L G C S E G L E E I R K S K V L V P L L I D L L R F G S T K G K E N S I T L L L G L C K D G G E E V A R R L L I N P R S I P S L Q S L S A D G S L K A R R K : 6 6 8 V v 2 9 3 3 3 0 0 1 : I E L L M D D K A G I T D D A L A V L A L L L G C P D G L E E I R K S R I L V P L L I D L L R F G S P K G K E N S I T L L L G L C K D G G E E V A R R L L L N P R S I P S L Q S L V A D G S L K A R R K : 6 6 8 B n A R C 1 : V G A L G - - E E A V A E K V A W V L G V M A T E S L G A E S I G R E E T V V T G L M E L M R C G R P R G K E K A I A T L L Q L C T A G G A V V T E K V V K T P A L A V L T R K L L L T G T D R A K R K : 6 4 4 7 2 0 M t P U B 1 : A D A L L R L L N R C C S Q P H H S L - : 6 9 4 L j S G A 0 1 9 0 2 : - - - - - - - - - - - - - - - - - - - - : - G m 0 2 g 4 3 1 9 0 : A D A V L R F L N R C C S Q P H H C V - : 6 5 3 L j B 0 2 K 2 0 : A D A L L R L L N R C C S Q P H H S L - : 6 7 8 P t 0 8 s 0 7 1 6 0 : A D A L L R L L N R E N I Q K R V - - - : 6 7 1 P t 1 0 s 1 9 3 3 0 : A D A L L R L L N R C C S Q S H N P V G : 6 8 8 V v 2 9 3 3 3 0 0 1 : A D A L L R L L N R C C S Q S H S T V G : 6 8 8 B n A R C 1 : A V S L S K V C K G C D Q N T Q R - - - : 6 6 1 U‐box ARM ARM ARM ARM ARM UND Supplemental Figure 1. Annotated alignment of PUB1 with Closely Related Proteins. Putative Mt PUB1 orthologues from Lotus japonicus (Lj), Glycine max (Gm), Populus trichocarpa (Pt) and Vitis vinifera (Vv) and ARC1 from Brassica napus (Bn) were used for the alignment. The position of the UND, U-Box and ARM repeats are shown. Similar alignments (Supplemental Datasets 1 and 2) were used to generate Supplemental Figures 4 and 5 and the alignments were trimmed between the arrows before phylogenetic analyses. Supplemental Data. Mbengue et al. (2010). Plant Cell 10.1105/tpc.110.075861