Top Banner
) ` r >7>3$8022+98)>>803$> E`prlwgi`eg` Slf% 38& El% 7& ~~% 399 $=12& 1990 @fw`si`r Wgi`eg` Fxh Gl~yrimkx © 1990 IBRL ^riex`h ie Mr` ax B rixai e% Aff rimkxw r`w`r s`h >7>3$8022,90 (9%0> / >%>> GKARAGX@RIZAXILE LC WYEA^XIG GLEE@GXILEW B@X]@@E GLRX@[ AEH H@@^ EPGF@I LC XK@ RAX G@R@B@FFPD IE SIXRL H% DLPMIELX* aeh B% K% M%m& K]IF@R Braie R`w`argk Iewxixpx`& Peis`rwixy lc Zcirigk& Apmpwx Clr`f$Wxraww` 1& ;>29 Ziirigk& W}ixz`rfaeh Abwxragx$$Iexrag`ffpfar r`glrhiemw }`r` pw`h xl gkaragx`riz` xk` iekibixlry wyea~w`w clrd`h by ^proiej` g`ffw le e`prle w ie xk` h``~ g`r`b`ffar epgf`i lc xk` rax% Xkiw }lro }aw ~`rclrd`h le l rmaelxy~ig g`r`b`ffar gpfxpr`w }k`r` cpegxileaf glee`gxilew b`x}``e ^proiej` g`ffw aeh h``~ g`r`b`ffar e`prlew ar` clrd`h h` elsl% Acx`r bflgoiem ilelxrl~ig `{gixaxlry adiel agih& aeh MABAA r`g`~xlrw }ixk 3$gyael$=$eixrl$ qpiel{afie`$2&7$hile`& H$2$adiel$0$~klw~klelsaf`rax` aeh b igpgpffie`& r`w~`gxis`fy& xk` dajlrixy lc h``~ g`r`b`ffar e`prlew cir`h w~lexae`lpwfy }ixklpx agglddlhaxile% Xkiw xleig ciriem }aw fie`arfy h`~`eh`ex le d`dbrae` ~lx`exiaf aeh }aw ablfiwk`h }ixk ky~`r~lfarizaxile% Baxk a~~figaxile lc dpwgidlf aeh bagflc`e r`s`rwibfy ky~`r~lfariz`h h``~ g`r`b`ffar epgf`i g`ifw% Ie xk` ~r`w`eg` lc `{gixaxlry adiel agih r`g`~xlr aexamleiwxw& ci`fh wxidpfaxile }ixkie xk` ^proiej` g`ff fay`r iehpg`h dlelwyea~xig iekibixlry ~lx`exiafw ie h``~ g`r`b`ffar e`prl ew xkax }`r` mrah`h aeh gld~f`x`fy bflgo`h by bigpgpffie`% Iekibixlry ~lx`exiaf ad~fixph`w }`r` elx daro`hfy r`hpg`h hpriem cawx r`~`xixis` wxidpfaxile lc ^proiej` g`ifw& aeh xk` r`wpfxiem ky~`r~lfarizaxile }aw elx acc`gx`h by xk` gld~`xixis` MABAa r`g`~xlr aexamleiwx GM^ 7078;% A wiemf` iekibixlry ~lx`exiaf x`d~lrarify iex`rrp~x`h xraiew lc agxile ~lx`exiafw iehpg`h ie h``~ g`r`b`ffar g`ffw by wklrx h `~lfariziem ~pfw`w% Xraiew lc cis` iekibixlry ~lwxwyea~xig ~lx`exiafw& `slo`h ax 2> Kz& iehpg`h a ky~`r~lfarizaxile }kigk xraewi`exfy bflgo`h xk` w~ lexae`lpw ciriem lc h``~ g`r`b`ffar g`ffw% Xk` `ccigi`egy xl bflgo agxile ~lx`exiaf hiwgkarm`w h `~`eh`h le xk` cr`qp`egy lc `slo`h iekibixlry ~lx`exiafw% Baxk a~~figaxile lc bigpgpffie` iehpg`h bprwx hiwgkarm`w ie xk` glexrlf wlfpxile% ]k`e xk` `{gixaxlry ad iel agih r`g`~xlrw }`r` ~kardaglflmigaffy bflgo`h& bigpgpffie` h`~lfariz`h h``~ g`r`b`ffa2" e`prlew iehpgiem wpwxaie`h agxile ~lx`exiaf hiwgkarm`w% Ie xk` ~r`w`eg` lc x`xrlhlxl{ie& bigpgpffie` ablfiwk`h dieiaxpr` iekibixlry ~lwxwyea~xig ~lx`exiafw aeh r`wpfx `h ie a d `dbrae` h`~lfarizaxile lc h``~ g`r`b`ffar g`ffw% ]` glegfph` xkax h``~ g`r`b`ffar e`prlew iwlfax`h crld wyea~xig ie~pxw hiw~fay a ~ag`dao`r$fio` agxisixy% Afxklpmk xk`w` e`prlew ~lww`ww MABAA aeh MABAB r`g`~xlrw& }` glecird xkax lefy MABA A r`g`~xlrw }`r` ieslfs`h ie xk` m`e`raxile lc iekibixlry ~lwxwyea~xig ~lx`exiafw& `s`e }ixk kimk cr`qp`egy wxidpfaxile% Xk` ad~fixph` lc `slo`h iekibixlry ~lx`exiafw }aw }`aofy cr`qp`egy$h`~`eh`ex& xkpw affl}iem a ~l}`rcpf iek ibixile lc xk` ~ag`dao`r$fio` agxisixy by xraiew lc `slo`h iekibixlry ~lwxwyea~xig ~lx`exiafw% Ah hixileaffy& w~lexae`lpw a eh dieiaxpr` iekibixlry ~lx`exiafw glexrlf xk` `{gixabifi xy lc h``~ g`r`b`ffar e`prlew by `{`rxiem a glexieplpw ky~`r~lfariziem xle`% Xk` e`prlew lc xk` h``~ g`r`b`ffar epgf`i +HGE) clrd xk` dlwx id~lrxaex `cc`r`ex ~axk}ay crld xk` daddafiae g`r`b`ffpd& }ixk xk` `{g`~xile lc xk` hir`gx glee`gxile crld ^proiej` g`ffw +^Gw) xl xk` hlrwaf ~arx lc xk` fax`raf s`wxibpfar epgf`pw +H`ix`rw epgf`i)% Aeaxld igaf wxphi`w kas` wkl}e xkax HGE r`g`is` ~rlj`gxilew crld iexriewig g`r`b`ffar ar`aw& aeh crld braiewx`d epgf`i% Xk` `{xriewig acc`r$ `ex ~axk}ay iw clrd`h by dlwwy 2u aeh gfidbiem cib`r a{le glffax`rafw% 9-1=-0¾Xk` iexriewig acc`r`ex ~axk}ay glewxixpx`w xk` glrxiglepgf`ar ~rlj`gxile& aeh iw clrd`h by a{lew lc ^Gw 2¾-81 }klw` x`rdieafw clrd gld~f`{ wyea~xig wxrpgxpr`w le HGE e`prlew% X D *Xl }kld glrr`w~leh`eg` wklpfh b` ahhr`ww`h% Abbr`siaxilew: GM^ 7078;& _^ $+7$adiel~rl~yf)$^$ hi`xkl{yd`xkyf$~klw~kleig agihT? GEQ[& 3$gyael$=$ eixrl$qpiel{afie`$2&7$hile`? H$A^S& H$2$Adiel$0$ ~klw~klelsaf`rax`? HGE& H ``~ g`r`b`ffar epgf`i? @AA & @{gixaxlry adiel agihw? I^W^w& Iekibixlry ~lwxwyea~xig ~lx`exiafw? ^Gw& ^proiej` g`ffw? XX[& x`xrlhlxl{ie% Xk` iekibixlry eaxpr` lc xk` glrxiglepgf`ar gle$ e`gxile kaw b``e h`dlewxrax`h by blxk `f`gxrl$ ~kywilflmigaf aeh bilgk`digaf wxphi`w% Iekibixlry ~lwxwyea~xig ~lx`exiafw +I^W^w) kas` b``e r`glrh`h ie iwlfaxile by wxidpfaxile lc k`diw~k`raf g`r`b`ffar glrx`{ ie 1)if)l 27-28 lr ie xiwwp` gpfxpr`% 77 Xk` I^W^w glpfh b` bflgo`h by bigpgpffie` 02 aeh by ~igrlxl{ie 78 wpmm`wxiem xkax MABA iw r`w~lewibf` clr xk` wyea~xig iekibixile lc xk` HGE g`ffw& aw ix iw clr xk` ^G$ d`hiax`h wyea~xig iekibixile lc xk` H`ix`rw epgf`i e`p rle w ) w-79 Bilgk`digaf d `awpr`d `exw xkax h`x`rdi$ eax`h xk` gleg`exraxile lc M AB A }ixkie ^Gw& 7= aeh }ixkie ^G a{le x`rdieafw 18 wp~~lrx xk` si`} xkax MABA iw xk` dajlr e`prlxraewdixx`r wyexk`wiz`h by ^Gw% Cprxk`rdlr`& xk` MABA$wyexk`wiziem `ezyd` +mfpxadig agih h`garbl{yfaw`)}aw wkl}e xl b` ~r`w`ex ie HGE& 17-87 aeh ~ridarify awwlgiax`h }ixk ^G a{le x`rdieafw% 01 Ie si`} lc xk` }`afxk lc haxa h`dlewxraxiem a rlf` clr MABA aw xk` xraewdixx`r lc xk` 39 9
14

D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

Apr 06, 2018

Download

Documents

GummyCola
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Page 1: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 1/14

u ) ^rmadle > 7 > 3 $ 8 0 2 2 + 9 8 ) > > 8 0 3 $ >

E`prlwgi`eg`Slf% 38& El% 7& ~~% 399 $=12& 1990@fw`si`r Wgi`eg`Fxh

Gl~yrimkx © 1990 IBRL^riex`h ie Mr` ax B rixaie% Aff rimkxw r`w`rs`h

>7>3$8022,90 (9%0> / >%>>

GKARAGX@RIZAXILE LC WYEA^XIG GLEE@GXILEW

B@X]@@E GLRX@[ AEH H@@^ EPGF@I LC XK@ RAX

G@R@B@FFPD IE SIXRL

H % D L P M I E L X * a e h B % K % M %m & K ] I F @ R

Braie R`w`argk Iewx i xpx`& Pe is`rwi xy lc Zci rigk& A pmp wx Clr`f $Wxraww` 1& ;>29 Zi i rigk& W}ixz`rfaeh

Abw x rag x $ $ Ie x rag` f fp f ar r`glrh iemw }`r` pw`h xl gkarag x`r iz` x k` i ek ib i xlry wyea~w`w clrd`h b y ^pro ie j `g`f fw le e`p rle w ie xk` h``~ g`r`b`ffar epg f`i lc xk` rax % Xkiw }lro }aw ~` rclrd` h le l rmae lxy~ig g`r`b`f fargp f xpr`w }k`r` cpegx ileaf glee`gx ilew b` x}``e ^pro ie j ` g` ff w aeh h``~ g`r`b`ff ar e`prle w a r` clrd`h h`

elsl% Acx`r b flgoiem ilelx rl~ig `{g i x a xlry adiel ag ih & aeh MABAA r`g`~xlrw }i xk 3$gyael$=$e i x rl$qpiel{af ie`$2 &7$h ile` &H$2$adiel$0$~klw~klelsaf`ra x ` ae h b igpgpf f ie`& r`w~`gxis`fy& xk` dajl ri xy lc h``~g`r`b`f fa r e`prle w c ir`h w~l exae`lpwfy }i xklpx agg ldd lhax ile % Xki w x le i g c ir iem }aw f i e`ar fy h`~`eh`exle d`dbrae` ~lx`e x i a f aeh }aw ablf i wk`h }i xk ky~`r~lfar i za x i le % Baxk a~~f i ga x i le lc dpwgidlf aehbagflc`e r`s`rwibfy ky~`r~ lfariz`h h``~ g`r`b`f far epgf`i g`i fw% Ie xk` ~r`w`eg` l c `{gi xaxlry ad iel agih

r`g`~xlr ae x amlei w x w & c i `fh w x idpfa x i le }i xk ie x k` ^p ro ie j ` g` ff f ay`r i ehpg`h d lelwye a~x i g i ek ib i xlry~lx`e x iafw ie h``~ g`r`b`ffar e `prl ew xkax }`r` mrah`h a eh gld ~f`x`fy bflgo`h by bigpgpf f ie`% Iek ibi xlry~lx`ex iaf ad~f i xph`w }`r` elx daro`hfy r`hpg`h hpriem cawx r`~`x i x is` wx idpfax ile lc ^proiej` g`i fw& aehxk` r`wp f x i em ky~`r~lfar i za x i le }aw elx a c c`g x `h by xk` gld~`x i x i s` MABAa r`g`~xlr ae x amlei w x GM^7078;% A w iemf` i ek ib i xlry ~lx `e x i a f x `d~lrar i f y i e x ` r rp~x `h x ra iew lc ag x i le ~lx`e x i a f w i ehpg`h ie h``~g`r`b`ffar g`f fw by wklrx h `~lfarizie m ~pfw`w% X raie w lc cis` iekib i xlry ~lwxwyea ~x ig ~lx`ex iafw& `slo`h ax2> Kz& ieh pg`h a ky ~`r~ lfariza x ile }kigk x raew i`ex fy bflgo`h xk` w~ lexa e`l pw ciriem lc h``~ g`r`b`ffarg`f fw% Xk` `ccigi`egy xl b flgo agx ile ~ lx`ex iaf hiwgkarm`w h `~`eh `h le xk` cr` qp`eg y lc `slo` h iekibi xl ry~lx`e x i a fw % B axk a~~ f i ga x ile lc b i gpgpf f ie` i ehpg`h bprw x h iwgkarm`w i e x k` gl ex rl f wl fp x ile % ]k` e xk``{gi xaxlry ad iel agih r`g`~xlrw }`r` ~kard aglflmiga f fy bflgo`h& bigp gpf f ie` h`~lfariz`h h``~ g`r`b f fa2"e`p rle w iehpgiem wpwxaie`h agx ile ~lx`ex iaf hiwgkarm`w% Ie xk` ~r`w`eg` lc x`x rlhlxl{ ie& bigpgpf f ie`ablf i wk`h diei a xpr` i ek ib i xlry ~lw x wyea~x i g ~l x `e xi a f w aeh r`wp f x h i e a d `dbra e` h`~lfar i za x i le lc h``~g`r`b`ffar g`ffw%

]` glegfp h` xkax h``~ g`r`b`ffar e` prl ew iwlfax`h crld wyea~ x ig ie~p xw hiw~fay a ~ag`dao` r$f io`agx isi xy% Af xklpmk xk`w` e`prlew ~lww`ww MABAA aeh MABAB r`g`~xlrw& }` gleci rd xkax lefy MABA Ar`g`~xlrw }`r` i eslfs`h ie x k` m`e`ra x i le lc i ek ib i xlry ~lw x wyea~x i g ~l x`e x i a f w & s`e }i xk k imk c r`qp`egy

w x idpfa x i le % Xk` ad ~f i x ph` lc `slo`h iek ib i xlry ~lx `e x ia f w }aw }`aofy c r`qp`egy$h`~`eh`ex & x kpwa f fl}iem a ~l}`rcp f i ek ib i x i le lc x k` ~ag`dao`r$ f i o` ag x is i x y by x ra iew lc `slo`h iek ib i xlry ~lw x wyea~x i g~lx`e x i a fw % Ah hi x i leaf fy & w~lexae `lpw a eh dieia xpr` i ek ib i xlry ~lx `e x i a f w glex rl f x k` `{g i xab if ix y lc h``~g`r`b`f fa r e`prle w by `{`r x iem a glex iepl pw ky~`r~lfar i z iem xle` %

X k ` e ` p r l e w l c x k ` h ` ` ~ g ` r ` b ` ff a r e p g f` i + H G E )

c l r d x k ` d l w x i d ~ l r x a e x ` c c ` r ` e x ~ a x k } a y c r l d x k `

d a d d a f i a e g ` r ` b ` f f p d & } i x k x k ` ` { g ` ~ x i l e l c x k `

h i r ` g x g l e e ` g x i l e c r l d ^ p r o i e j ` g ` ff w + ^ G w ) x l

x k ` h l r w a f ~ a r x l c x k ` f a x` r a f s ` w x i b p f a r e p g f ` p w

+ H ` i x ` r w e p g f ` i) % A e a x l d i g a f w x p h i ` w k a s ` w k l } e x k a x

H G E r ` g ` is ` ~ r l j ` g x i l e w c r l d i e x r i e w i g g ` r ` b ` f f a r

a r ` a w & a e h c r l d b r a i e w x ` d e p g f ` i % X k ` ` { x r i e w i g a c c` r $` e x ~ a x k } a y i w c l r d ` h b y d l w w y 2u a e h g f i d b i e m c i b ` r

a { l e g l f f a x ` r a f w % 9 -1 =-0 ¾ X k ` i e x r i e w i g a c c ` r ` e x ~ a x k } a y

g l e w x i x p x ` w x k ` g l r x i g l e p g f ` a r ~ r l j ` g x i l e & a e h i w

c l r d ` h b y a { l e w l c ^ G w 2¾-81 } k l w ` x ` r d i e a f w c l r d

g l d ~ f ` { w y e a ~ x i g w x r p g xp r ` w l e H G E e ` p r l e w % XD

*Xl }kld glrr`w~leh`eg` wklpfh b` ahhr`ww`h %Abbr`siaxilew: G M ^ 7 0 7 8 ; & _^ $ +7 $ a d i e l ~ rl ~ y f) $^ $

h i ` xkl{yd`xkyf $~klw~kl eig ag ihT ? GEQ[ & 3 $gyael$=$ei x rl$qp iel{a f ie`$2&7 $hile `? H$A^S& H$2$Adiel$0$~klw~kl elsaf`ra x ` ? HGE & H ``~ g`r`b`f fa r epgf` i ? @AA &@{gi x a xlry adie l ag ihw ? I^W^w & Iek ib i xlry ~lw x wyea~x i g~lx`ex iafw? ^Gw& ^proiej` g`f fw? XX[& x`x rlhlxl{ie%

X k ` i e k i b i x l r y e a x p r ` l c x k ` g l r x i g l e p g f ` a r gl e $

e ` g x i l e k a w b ` ` e h ` d l e w x r a x ` h b y b l x k ` f `g x rl $

~ k y w i l f l m i g a f a e h b i l g k ` d i g a f w x p h i ` w % I e k i b i x l r y

~ l w x w y e a ~ x i g ~ l x ` e x i a f w +I ^ W ^ w ) k a s ` b ` ` e r ` g l r h ` h i e

i w l f a x i l e b y w x i d p f a x i l e l c k ` d i w ~ k ` r a f g ` r ` b ` f f a r

g l r x ` { ie 1) i f ) l 27 - 28 lr i e x i w w p ` g p f x p r ` % 77 X k ` I^ W ^ w

g l p f h b ` b f l g o ` h b y b i g p g p f f i e `02 a e h b y ~ i g r l x l { i e 78

w p m m ` w xi e m xk a x M A B A i w r ` w ~ l e w i b f` c l r x k ` w y e a ~ x i gi e k i b i x i l e l c x k ` H G E g ` ff w& a w i x iw c l r x k ` ^ G $

d ` h i a x ` h w y e a ~ x i g i e k i b i x i l e l c x k ` H ` i x ` r w e p g f ` i

e ` p r l e w ) w-79 B i l g k ` d i g af d ` a w p r ` d ` e x w x k a x h ` x ` r d i $

e a x ` h x k ` g l e g ` e x r a x i l e l c M A B A } i x k i e ^ G w & 7= a e h

} i x k i e ^ G a { l e x ` r d i e a f w 18 w p ~ ~ l r x x k ` s i ` } x k a x

M A B A i w x k ` d a j l r e ` p r l x r a e w d i x x ` r w y e xk ` w i z` h b y

^ G w % C p r x k ` r d l r ` & x k ` M A B A $ w y e x k ` w i z i e m ` e z y d `

+ m fp x ad i g ag ih h ` g a r b l { y f a w ` ) } a w w k l } e x l b `

~ r ` w ` e x i e H G E & 17-87 a e h ~ r i d a r i f y a w w l g i a x ` h } i x k

^ G a { l e x ` r d i e a f w % 01

I e s i ` } l c x k ` } ` a f x k l c h a x a h ` d l e w x r a x i e m

a r l f ` c l r M A B A a w x k ` x r a e w d i x x ` r l c x k `

39 9

Page 2: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 2/14

=> > H Dl p m ie l x ae h B K M, ik }i f` r

g l r x i g l e p g f ` a r ~ r l j ` g x i l e & x k ` ~ a p g i x y l c i e c l r d a x i l e

l e x k ` i e k i b i x l r y w y e a ~ x i g g l e e ` g x i l e w i w w p r ~ r i w i e m

X k ` m a ~ i e l p r p e h ` r w x a e h i e m g a e b ` a x x r i b p x ` h x l x k `

h i c ci g p fx y l c ` s l o i e m i e k i b i x l r y w y e a ~ x i g ~ l x ` e x i a f w i e

a g p x ` g ` r ` b ` f f a r w f i g` w } k ` r ` w i m e i c ig a e x e p d b ` r w l c

^ G a { l e w a r ` g p x h p r i e m x k ` ~ r ` ~ a r a x i l e l c xk ` w fi g `

X k ` a i d l c xk ` ~ r ` w ` e x } l r o } a w x l g k a r a g x ` r i z `

c p e g x il e a f f y x k ` ^ G $ i e h p g ` h i e k i b i xi l e l c H G E g ` ff w&x a o i e m a h s a e x a m ` l c l r m a e l x y ~ i g g ` r ` b ` f f a r g p f x p r ` w

} k ` r ` ^ G w c l r d g l e e ` g xi l e w } i x k H G E e ` p r l e w h`

elsl%10 A e ` f ` g x r l ~ k y w i l f l m i g a f a ~ ~ r l a g k } a w p w ` h x l

c i rw x fy h ` x ` r d i e ` x k ` l r i m i e l c x k ` w ~ l e x a e ` l p w c i r ie m

a g x i s i x y l c H G E e ` p r l e w W ` g l e h f y & } ` g k a r a g x ` r i z ` h

x k ` M A B A ` r m i g r ` g ` ~x l r w i e s l f s ` h i e x k ` i e k i bi x l r y

w y e a ~ w ` w& a e h } ` w xp h i ` h x k ` e a x p r ` a e h h y e a d i g w l c

i e k i b i x l r y w y e a ~ x i g ~ l x ` e x i a f w C i e a f f y & x k ` r l f ` ~ f a y ` h

b y I ^ W ^ w i e x k ` d l h p f a x i l e l c a g x i l e ~ l x ` e x i a f

h i w g k a r m ` a e h i e x k ` g l e x r l f l c x k ` ` { g ix a b i f ix y l c

H G E g ` f fw } a w i e s ` w x i m a x ` h

@ [ ^ @ R I D @ E X A F ^ R L G @ H P R @ W

^r`~araxile lc g`r`b`ffar wfig` gpfxpr`w

Lrm ae l xy ~ ig gp f xp r` w }` r` ~ r`~ a r`h c rl d g` r`b ` f far`dl s `h c rl d e `}$b l re ra x ~ p ~ w +p ~ xl l e ` h ay l fh )+]iwxar & Bilflmigaf R`w`argk Fablraxlri`w & Ci i f f iewhlrc &W}ixz`rfaeh) Xk` g`r`b`ffpd } aw h iww`gx`h p eh`r aw`~xigglehix il ew aeh ~arawamix xa f w f ig`w lc 8>> 'd xk igoe`ww }`r`gpx }ixk a Dgff}aie x iwwp` gkl~~`r Iehis ihpaf w f ig`w }`r`

`db `hh`h le m faww gls`rwf i~w ie g fl x x`h gkigo`e ~ fawda& ae hgpfxpr`h by d`aew lc xk` rl f f`r xpb ` x`gkeiqp` u0-u3 Xk`gpfxpr`w }`r` c`h leg` a }``o }ixk a d`hipd glewiw x iem lc2 0 % k `a x $ ie ag x is a x`h k l rw` w` rp d& 0 > % @am f` - w b a wa fd`h ip d& 2 0 % Kae o - w b a fae g`h wa f x wl fp x il e & gl e xa ie ie m777 d D H$mfpglw` aeh > 1 dD mfpxadie` G pfxpr`w }`r`p w`h a c x` r a ~ ` r il h l c x}l xl cl p r }``o w ie sixrl& h p r ie m

}kigk xk`y }`r` daiexa ie`h a x 73¾G

@f`gxrl~kywilflmy

Clr ` f`gxrl~kywil flmigaf r`glrhiemw& gpfxpr`w }`r` xraew$c` r r`h xl a x `d~ `ra xp r` gl e x rl f f`h +7> ¾G) gk adb `r d l p e x`hl e xk ` w xam ` l c ae ie s ` r x`h dig rl wgl ~ ` Gp f xp r` w }` r`glexieplpwfy ~`rcpw`h }ixk ae `{xrag`ffp far wl fp xile+Xy rl h ` wl fp x il e ) gl e xa ie ie m ie dD: EaGI & 1 7 = ? OGI & 2 = ?

GaG12& 2;? D mG12& 2? EaK2^L 8& >70? E aK G L 7& 10 aehmfpglw`& 00 ax ~K =8

HG E }`r` ih `e xi c i`h b y xk ` i r fl ga x il e }i xk ie xk ` gp f xp r`aeh e`prlew }`r` id~af`h }ixk wkar~ ` f`gxrlh`w c i f f`h}ixk I D ~lxawwipd d`xkyfwp fca x` wl fp xile +r`w iwxaeg`80$=> DCu) Gprr `ex } aw ie j`gx`h ie xl xk` g`f f }ixk xk` agx is`b r ih m ` g i rgp ix l c xk ` ad~ f i c i ` r +A{ l g fad~ 2 A& A { l e Ie w x rp $d`e x Ie g & Cl w x` r G i xy & GA& P W A ) & }k l w` b a fae g` }aw

dl e i xl r`h s ia k y ~ ` r~ l fa r iz ie m s l f xam ` w x`~ w D`db rae `~lx`exia fw }`r` c i f x`r`h a x 2>oKz& h im ixz`h a x 0oKz+Fab daw x` r ae a fl m $h im ixa f gl e s ` r x` r & A{ l e Ie w x rp d `e x Ie g )ae h w xl r`h l e f ie ` l e a gl d~ p x` r k a rh h i wo ae h l e h im i xa fs ih`l gaww`xx` +8> oKz) clr cprxk`r aeafywiw Ha xa aeafywiw}aw ~ ` r cl rd`h }i xk ~ Gfad~ wl c x}a r` +A{ l e Ie w x rp d`e x &Ieg) Wyea~xig r`w~lew`w }`r` `slo`h }ixk dlel~lfar w x id$pfi +8>$90,uA hpriem 2>>,uw) h`fis`r`h s ia a m faww ~ i~`xx`+1$2 'd xi~ & c if f`h }ixk Xyrl h` wlfp xile) ~ fag`h ie xk` ^Gfay`r Hrp mw }`r` a~~fi`h by baxk a~~figax ile Affwpbwxaeg`w }`r` ~r`~a:r`h aw wxlgo wlfpxilew ie hiwxiff`h}ax`r & aeh }`r` h i fp x`h xl xk`ir c ieaf gleg`exra x ile ie`{ x rag` f fp fa r wl fp x il e & jp w x b ` cl r` p w` + $ ) $Bag fl c`e + c f $~ $gk fl rl ~ k `e y f $MAB A) & ae h GM ^ 7 0 78 ; _^$ +7 $adie l ~ rl ~ y f )$^$h i` xk l { y d` xk y f $~ k l w~ k l e ig ag ih T }` r` ~ rl s ih `h b y Gib aM`imy Fxh +Baw`f & W}ixz`rfaeh) H$2$A diel$0$~ klw~klel $

saf`ra x` +H$A^S) aeh 3$gyael$=$eixrl$qpiel{afie`$2 &7$h il e ` +GEQ[) }`r` ~ p rgk aw`h c rl d Xl g r i w E`p radie+PO) Bigpgpffie` d `xklgkfl rih` +r`c`rr`h xl aw b igpgpff ie`ie xk` x`{x) }aw wyexk`wiz`h crld bigpgpff ie` +Cfpoa G k`d i`AM & Bpgkw& W}ixz`rfaeh) aeh d pwgidl f }aw a fwl ~prgkaw`hc rl d Cfp o a X` x rl h l xl { ie +XX[) }aw ~ p rgk aw`h c rl dWaeoyl +Ja~ae) Aff w xa x iw x iga f r`wpfxw ar` `{~r`ww`h awd`ae / w xae h a rh h `s ia x il e

H`x`rdieaxile lc xk` d`dbrae` ~lx`exiaf hpriem wpwxaie`hciriem lc h``~ g`r`b`ffar epgf`i g`ffw

Xk ` `{ ag x s a fp ` l c xk ` d`db rae ` ~ l x`e x ia f l c HG E g` ff whpriem wp wxa ie`h c ir iem& ` i xk`r w~lexae`l pw lr ie r`w~lew` xlgl e x ie p l p w ie j`g x il e l c h `~ l fa r iz ie m gp r r`e x& gl p fh e l x b `hir`gxfy r`ah crld xk` ad~fic i r @wx id axile lc xk` d`d$b rae ` ~ l x`e x ia f } aw ~ ` r cl rd`h }i xk xk ` cl f fl }ie m ~ rl xl gl fD`dbrae` ~lx`exia f }aw h`cie`h aw xk` as`ram`h h icc`r`eg`l c ~ l x`e x ia f b ` x}``e xk ` da{ ida f a c x` rk y ~ ` r~ l fa r iza x il eclffl}iem agxile ~lx`exia fw aeh xk` le w`x lc xk` e`{x cawxEa/$h `~ `e h `e x w~ io `

Wxaieiem lc e`rs` g`f fw ~r`w`ex ie wfig` gpfxpr`w

Fis ie m Gw ae h HG E e `p rl e w }`r` id~ a f`h p e h ` r s i wp afglexrl f }ixk wkar~ ` f`gxrlh`w glexa ie iem 0% bilgyxie h iw$wlfs`h ie > >0 D Xriw bpcc`r`h$wafie` Ky~` r~lfariz iem gpr$

r`ex ~pfw`w +7>> dw& 2 Kz) }`r` a~~fi`h xl xk` g`f fw hpriem7>u> die Acx`r I k ~lwx$ ie j`gx ile wprsisaf a f fl}iem h iccp$w il e l c b il gy xie xk rl p m k l p x xk ` h `e h r i x i g x r`` ae h xk ` a{ l e &gpfxpr`w }`r` c i{`h ls`reimkx ie 8% ~araclrdafh`kyh` aeh>0% mfpxara fh`kyh` Xk` r`agxile }aw ~`rclrd`h pwiem aeABG ~`rl{ihaw` o i x +S`gxlr fablraxlri`w Ieg & Bprf iemad`&PWA ) ae h h iadie l b `e z ih ie ` a w gk rl dam `e & cl f fl }`h b yXlfpih ie` Bfp` +> 1%) glpex`rw xa ie iem Gpfxpr`w }`r` xk`eh`kyhrax` h xk rlpmk ae awg`ehiem w`r i w lc ` xkaelf wl f$p x il e w ^k l xl m ra~ k w }`r` x ao `e p w ie m a Z` i w w A{ il ~ k l xdigrlwgl~`

R@WPFXW

G l r x i g l e p g f ` a r ~ r l j ` g x i l e w i e w f i g ` g p f x p r ` w

X k ` ~ r ` w ` e x w x p h y } a w g a r r i ` h l p x } i x k l r m a e l x y ~ i g

g ` r ` b ` f f a r w fi g ` g p f x p r ` w I e x k i w ~ r ` ~ a r a x i l e & f i s i e m

^ G w a e h e ` p r l e w l c x k ` H G E g a e b ` i h` e x ic i `h b y xk `

w i z` a e h w k a ~ ` l c x k ` i r w l d a x a & a w } ` ff a w b y x k ` ir

f l g a x i l e } i x k i e x k ` g p f x p r ` w 7-2= D l r ` l s ` r & i e x r a g ` f f p $

f a rf y fa b ` f` h e ` p r l e w l c xk ` H G E a e h ^ G w w k l }

g k a r a g x ` r i w x i g h ` e h r i x i g a r b l r i z a x i l e w + C i m 1 A & B )

H p r i e m x k ` c i r w x x } l } ` ` o w ie s i x rl & ^ G w m r l } a { l e w

x l } a r h x k ` g ` e x ` r l c x k ` g p f x p r ` } k ` r ` x k ` y x ` r d i e a x `

l e H G E e ` p r l e w & x kp w c l r d i e m a e id ~ r ` w w is ` ie sixrl

a e a f l m l c x k ` g l r x i g l e p g f ` a r ~ r l j ` g x i l e X l w x p h y x k `

~ a x k } a y l c i e h i s i h p a f a { l e w c r l d x k ` g l r x ` { x l x k `

H G E & ^ G w } ` r ` i e x r a g ` f f p f a r f y i e j ` g x ` h } i x k b i l g y x i e

a e h x k ` g p f x p r ` w x k ` e w p b j ` g x ` h x l k i w x l g k ` d i g a fa e a f y w i w + C i m 1 G ) X k ` w ` d l r ~ k l f l m i g a f w x p h i ` w

g f ` a r f y h ` d l e w x r a x ` x k a x g p f x p r ` h ^ G w ~ r l j ` g x x k ` i r

a { l e w x l x k ` a r ` a l c H G E a e h a ~ ~ ` a r x l ` w xa b f iw k

w y e a ~ x i g g l e x a g x w } i x k H G E e ` p r l e w + C i m 1 G &

ie w` r x )

M ` e ` r a f ` f ` g x r l ~ k y w i l f l m i g a f g k a r a g x ` r i w x i g w

X k i w w x p h y } a w g a r ri ` h l p x l e 0 > H G E e ` p r l e w

x k a x k a h i e ~ p x r ` w i w x a e g` w ` { g ` ` h i e m 7 > D c u a e h x i d `

g l e w x a e x w 5 3 d w L e f y H G E g ` f fw h i w ~ f a y i e m a g x i l e

~ l x ` e x i a f w & ` i x k ` r w ~ l e x a e ` l p w f y & l r i e r ` w~ l e w ` x l

i e j ` g x i l e l c h ` ~ l f a r i z i e m g p r r ` e x & } ` r ` w ` f ` g x` h

Page 3: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 3/14

G l r x i g l e p g f ` a r ~ r l j ` g x i l e w i e g ` r ` b ` f f a r w f ig ` g p f x p r ` w = >1

C i m % I % D l r ~ k l f l m i g a f ` s i h ` e g ` c l r x k ` ~ r ` w ` e g ` l c g l r x i g l e p g f ` a r ~ r l j ` g x i l e w i e g ` r ` b ` f f a r w fi g ` g p f x p r ` w %

I A ) W i e m f ` ^ G i e j ` g x ` h } i x k b i l g y x i e w k l } ` h x k ` g k a r a g x ` r i w x i g h ` e h r i x i g a r b l r i z a x i l e % + B ) W i e m f`

b i l g y x i e $c i f f` h H G E e ` p r l e % + G ) L s ` r s i ` } l c g p f x p r ` a w w ` ` e } i x k h a r o $ c if f ` h i ff p d i e a x i l e % X k ` a { l e w l c

x } l ^ G w f a b ` f ` h } i x k b i l g y x i e a r ` w ` ` e xl x ` r d i e a x ` i e x k ` g ` e xr a f ~ a r x l c x k ` g p f x p r` } k ` r ` x k ` H G E

e ` p r l e w a r ` f l g a x ` h % A h ` x a i f l c x k ` x ` r d i e a f a r b l r i z a x i l e l c xk ` ^ G w i w if f p w x r a x ` h i e x k ` i e w ` r x% E l x ` x k a x

x k ` w y e a ~ x i g b l p x l e w a ~ ~ ` a r x l b ` f l g a x` h l e w l d a x a l c H G E g ` ff w a e h l e h ` e h r i x ` w l c H G E e ` p r l e w %

G a f i b r a x i l e b a r g l r r ` w ~ l e h w x l 1 > >1 u d i e A a e h B & x l 2 > > , u d i e G & a e h x l 0 > u d i e xk ` i e w `r x %

Page 4: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 4/14

=> 2 H D lpmie l x aeh B K M , ik}if` r

Xab f` 1 @f`gxrl~kywilflmigaf gkarag x r iwxigw lc h``~ g`r`b`ffar e pgf`i e`p rle w

Ie~ p x X id ` Caw x Ea ý w~ io` W~ io` W~ io`G`ff r`wiwxaeg` gle wxae x xkr`wklfh k`imkx hp raxi leEl +dcu) +dw) +dS) +dY) +dw)

1 7; ; 8 $8 1 00 > 32 82 f> $8 1 80 > 07 00 ;3 $ 8 > 8; > =8 08 1> 3 $7 ; 83 > ;

0 8> ; 3 $ 7 3 8> > 93 79 12 8 $ 8 > 0> > 3= 0> 172 $ 7 = 7; >=; 01 11 $ 7 3 87 >09 73 120 $ 7 9 82 >0

1> 7; = 8 $ 7 = 80 > 01 1 88 39 $ 80 73 >;12 82 127 $ 7 9 81 >;17 78 1>= $ 8 0 73 >=18 0= 11 $ 78 8; >010 8> ;0 $8 1 77 >313 73 1>; $ 7 0 88 >91= 73 32 $ 7 0 81 >81; 3> 120 $ 8 > 80 >019 8> ; 0 $ 8 > 8> > 32> 02 99 $ 79 83 >8

D ` a e 8 8 7 / = 9 1 > / 2 $ 7 9 / 7 8 2 7 / 0 ; > 3 / > 1 0/ W H Raem ` 78$$3> 3 2 $17 2 $8 0 xl $7 0 77$00 > 88) 9

X a b f ` 1 w p d d a r i z ` w x k ` ` f ` g x r i g a f g k a r a g x ` r i w x i g w l c 2 >

r a e h l d f y w ` f `g x `h H G E e ` p r l e w I e ~ p x r` w i w xa e g `

a e h d ` d b r a e ` x i d ` g l e w x a e x } ` r ` h ` x ` r d i e ` h b y

1 8 > d w k y ~ ` r ~ l f a r i z i e m ~ p f w` w X k ` w ~ i o ` k ` i m k x } a w

d ` a w p r ` h a w h i cc ` r `e g ` b ` x } ` ` e a g x i l e ~ l x ` e x i a f ~ ` a o

a e h x k ` c a w x E a / $ h ` ~ ` e h ` e x w ~ i o ` x k r ` w k l fh X k `

h p r a x i l e l c x k ` w ~ io ` } a w h ` x ` r d i e ` h a x k a f c d a { i d a f

a d ~ f i x p h ` D ` a w p r ` d ` e x l c r ` w x ie m ~ l x ` e x i a f } a w

g l d ~ f i g a x ` h b y x k ` c a g x x k a x x k ` d a j l r i x y l c H G E

e ` p r l e w c i r` h w ~ l e x a e ` l p w f y C l r x k i w r ` a w l e & s a f p ` w

l c r ` w x i e m d ` d b r a e ` ~ l x ` e x i a f } ` r ` e l x i e g f p h ` h i e

x k ` x a bf ` I e xk ` ~ r ` w ` e g` l c X X [ + > 0 ' D ) & x k `

r ` w x ie m d ` d b r a e ` ~ l x ` e x i a f } a w $ 0 3 / 2 8 d S

+ e 4 9 ) %

X l x ` wx } k ` x k ` r H G E g ` ff w ` { k i b i x ie x r i e w i g w ~ l e $

x a e ` l p w h i w g k a r m ` w & g p f x p r ` w } ` r ` ~ ` r c p w ` h } i x k a

w l f p x i l e g l e x a i e i e m G E Q [ + 2> ux D ) & H $ A ^ S

+ 8 > , u D ) a e h b i g p g p f f i e ` + 8 > ,u D ) X k ` w ~ l e x a e ` l p w

c i ri e m c r ` q p ` e g y + l b x a i e ` h } i x k l p x i e j ` g x i l e l c g p r $

r ` e x i e x l x k ` H G E g ` f f ) a s ` r a m ` h 2 2 \\\ ; K z a x a

d ` d b r a e ` ~ l x ` e x i a f l c $ 8 7 $I$ 7 d S + e 4 ;) X k `e p d b ` r l c w ~ i o `w f i e ` a r f y i e g r ` a w ` h } i x k x l e i g

h ` ~ l f a r i z a x i l e l c x k ` g ` f f + l b x a i e ` h s i a g l e x i e p l p w

i e j ` g x i l e l c ie g r ` d ` e x ` h ~ l w i x is ` g p r r ` e x ) a e h x k `

d a { i d a f w p w x a i e ` h c i r ie m k a h a r a x ` l c 8 1 / = K z a x

a d ` d b r a e ` ~ l x ` e x i a f l c $ 7 3 \\\ 0 d S + C i m 2 ) C a w x

E a / $ h ` ~ ` e h ` e x w ~ io ` w } ` r` i e a g x i s a x ` h } k ` e x k `

d ` d b r a e ` ~ l x ` e x i a f r ` a g k ` h $ 2 9 \\\ 2 7 d S X k ` w p w $

x a i e ` h c i r i e m } a w w x a b f ` c l r ` { x ` e h ` h x i d ` ~ ` r i l h w

+ 5 2 d i e ) & ` s ` e a x k i m k r a x `w l c a g x i l e ~ l x ` e x ia f

h i w g k a r m ` w & w p m m ` w x i e m x k a x x k ` w ~ i o i e m a g x i s i x y l c

H G E e ` p r l e w h ih e l x a g g l d d l h a x ` E ` i x k ` r w~ l e $

x a e ` l p w a g x is i x y e l r d ` d b r a e ` l w g i f fa x i l e w } ` r `

l b w ` r s ` h a x d ` d b r a e ` ~ l x ` e x i a f w d l r ` e ` m a x i s ` x k a e

$ 3 > d S

A h i c c ` r ` e x ~ a x x ` r e l c i e x r i e w i g a g x i s i x y } a w l b $

w ` r s ` h i e x k r` ` l p x l c 1 9 g ` f fw + h a x a e l x w k l } e ) X k `

a g x i s i x y h i h e l x c l f f l } x k ` r ` m p f a r ~ a x x ` r e h ` w g r i b ` h

a b l s ` & b p x a ~ ~ ` a r ` h a w b p r w x w l c a g x i l e ~ l x ` e x ia f w

X k i w l w g if fa x lr Y ~ a x x ` r e } a w a b w ` e x c l r d ` d b r a e `

~ l x ` e x i a f w d l r ` e ` m a x i s ` x k a e $ 3 > d S a e h w k i c x `h x l

w p w x a i e ` h a g x i s ix y h p r i e m w x r l e m h ` ~ l f a r i z a x i l e l c

x k ` w ` e ` p r l e w

G karag x ` r i za xi le l c ~lw x w yea~ x i g M A B A r`g`~ xlr w le

h``~ g`r`b`ffar epgf`i g`ffw

B a x k a ~ ~ f i g a x i l e l c M A B A + 1 > > , u D & 7 > w ) ~ r l $

h p g ` h a x r a e w i ` e x k y ~ ` r ~ l f a r i z a x i l e + 1 1 ; / 3 ? 3 d S )

l c H G E g ` ff w + e 4 9 ) X k ` a d ~ f i x p h ` l c x k ` r ` w ~ l e w `

s a r i ` h g l e w i h ` r a b f y c r l d g ` f f x l g ` f f & ~ r l b a b f y h p ` x l

p ~ x a o ` l c M A B A G l $ a ~ ~ f i g a x il e l c M A B A a e h

x i a m a b i e ` + 2 0 ,u D & 7 > w )& a e i e k i b i x l r l c M A B A p ~ $

x a o ` u i e g r ` a w `h x k ` a d ~ f i x p h ` l c x k ` k y ~ ` r ~ l f a r i z $

a x i l e b y = 9 / 7 3 % i e c l p r g ` ff w + h a xa e l x w k l } e ) I e l r h ` r x l g k a r a g x ` r iz ` x k ` x y ~ ` w l c M A B A r ` g `~ $

x l r w ~ r ` w` e x l e H G E e ` p r l e w & } ` a ~ ~ f i ` h d p w g i d l f &

a M A B A a e a f l m x k a x iw e l x a w p b w x r ax ` c l r M A B A

p ~ x a o ` & a e h x k ` M A B A B r ` g ` ~ x l r a m l e i w x& b a g f l c ` e &

i e x k ` ~ r `w ` e g ` l c ` ix k ` r X X [ + > 0 ' D ) & l r i l e l x r l ~ i g

` { g i x a xl r y a d i e l a g i h + @ A A ) r ` g ` ~ x l r a e x a m l e i w x w

+ G E Q [ & 2 > , x D a e h H $A ^ S & 8 > ' D ) B r i` c

b a x k a ~ ~ f i g a x i l e l c d p w g i d l f + 0 ' D h p r i e m 7 > w &

e 4 = ) g a p w ` h a x r a e w i ` e x a e h r ` s ` r w i b f ` k y ~ ` r $

~ l f a r i z a x i l e l c H G E g ` ff w l c 1 = 7 / 7 d S a x a

k l f h i e m ~ l x ` e x ia f l c $ 0 > d S + C i m 7 A & p ~ ~ ` r x r a g` &

f `c x) X k ` d p w g i d l f $ i e h p g ` h k y ~ ` r ~ l f a r i z a x i l e } a w

Page 5: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 5/14

Gl r x i gl e p g f `a r ~ rl j `g x i l e w i e g` r`b ` f f a r w fi g` gp f x p r` w = > 7

A u

D ^ : $ 3 > d S

I

C : 22 K z

D ^ : $7 9 d S H ` : > e A

C : 81 % 0 K z

D ^ : $7 7 d S

2 > d S

11 > > d w

B 0 >

8 >

7 >

2 >

1 >

x •

` L u , , ,

` _ , ,

• A J , •J + T L

`e , ^* •L B u N /

J _7

I x W •

w Qx •

x* i i i

$ 0 > $ 8 > $ 7 >

D` d bra e` ~lx ` ex ia f + d S )

> i

$ 3 > $ 2 >

Ci m % 2 % ^ag`dao ` r $ f i o ` ag x i s i x y l c H GE e `p rl e w % +A ) Ie x k ` ~ r` w`e g` l c @A A r`g`~ x l r ae x am l e i w x w

+ G E Q [ & 2 > ' D ? l $ A ^ S & 8 > ~ D ) a e h b i g pg p f fi e ` + 8 > , u D ) & x k ` i f fp w x r a x` h H G E e ` p r l e h i w g k a r m` h

w ~ l e x a e ` l p w f y a x a c r ` q p ` e gy l c 2 2 K z a x x k ` d ` d b r a e ` ~ l x ` e x i a f +D ^ ) l c $ 7 7 i e S % A g x i l e ~ l x ` e x i a f

h i wgk a rm ` } aw i e g r`a w`h x l a d a{ i d a f ra x ` l c 8 1 %0 K z } k ` e x k ` g` ff } aw h `~ l f a r i z`h } i x k a x l e i g i e j `g x i l el c ~ l w i x i s ` g p r r ` e x % E l x ` x k ` a b w ` e g ` l c a g g l d d l h a x i l e l c c i r i e m & ` s ` e } i x k f a r m ` h ` ~ l f a r i z a x i l e w %

K y ~ ` r ~ l f a r i z a x i l e l c H G E e ` p r l e w i e h p g ` h b y w p w x a i e ` h i e j ` g x i l e l c e ` m a x i s ` g p r r ` e x a b l f i w k ` h x k `w~ l e x ae `l p w ` f `g x r i g a f a g x i s i x y % +B) R` f a x i l e wk i ~ b ` x } ``e d`db rae ` ~ l x `e x i a f ae h c r`q p `e gy l c w x `ah y $w x a x ` ag x i l e ~ l x `e x i a f h i wgk a rm `w l b x a i e `h i e ` i m k x H G E g` f fw +@agk H G E g` ff iw r`~ r` w`e x `h b y a h i c c` r`e xwy db l f ) % X k ` ~ l i e x w } ` r` c i x x `h b y a f i e ` a r r`m r` ww i l e +h a wk `h f i e ` ) } i x k a gl r r` f a x i l e gl ` x c ig i `e x l c > %9 2 %

Xk ` w f l ~ ` s a f p ` } aw 1 %; K z , d S %

Page 6: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 6/14

= > 8 H D l p m i e l x a e h B K M , i k } if ` r

Ju XX[ >%0 ~D

#IR

8d

+9"3

Q %

@

5

Le&

1> >

Dpwgidlf 0 ~D

0 >

I I

e4= e4= e40

0 d S

I

Dpwgid lf / Bigpgpf f ie` 8> ~D

u Dpwgidlf

B Dpwgid lf / Bigpgpf f ie`

u T R`gls`ry

1$1"[ >%0 IJD

/H G

0 d S

I

2> w

8 > w

gL44

d

q++%9"3

IL

L%

@

5

e&

Bagflc`e 1>> ~D B a h l c ` e / GM^ 7078;& 1 dD

1>>%

0 >

> $ $

H Bagflc`e

B B ag flc`e / G M^ 7078;

R`gls`ry

e 4 3 e 4 3 e 4 0

C i m 7 G k a r a g x ` r iz a x i l e l c ~ l w x w y e a ~ x i g M A B A ` r m i g r ` g ` ~ xl r w l e H G E e ` p r l e w + A ) X k ` ~ r ` w `e g ` l c

M A B A A r ` g ` ~ x l r w } a w i e s ` w x im a x ` h ie x k ` ~ r ` w ` e g ` l c X X [ + > 0, u D ) B a x k a ~ ~ f i g a x i l e l c d p w g i d l f + 0 ~ D )

~ r l h p g ` h a x r a e w i ` e x a e h r ` s ` rw i b f` k y ~ ` r ~ l f a r i za x i l e } i x k a g l e g l d i x a e x r ` h p g x i l e l c x k ` i e ~ p x r ` w iw x a e g `

_ p ~ ~ ` r f` c x x r ag ` K l f h i e m ~ l x ` e x i a f +K ^ ) : $ 3 > d S T B i g p gp f fi e ` + 8 > ' D ) w x r l e m fy a e x a m l e i z ` h x k `

d p w g i d l f $ i e h p g ` h k y ~ ` r ~ l f a r i z a x i l e + p ~ ~ ` r x r a g ` & ri m k x ) X k ` w x a x iw x i g a f r ` w p f x w a r ` w p d d a r i z ` h i e x k `

k i w x l m r a d + f l } ` r ~ ae ` f ) } k ` r ` x k ` a d ~ f i x p h ` w l c x k ` g l e x r l f M A B A A r ` w ~ l e w ` w } ` r` e l r d a f i z ` h a x 1 > > %

+ c ir w x g l f p d e ) X k ` w ` g l e h g l f p d e w k l } w x k ` ` c c `g x l c b i g p g p f f ie ` l e x k ` d p w g i d l f $ i e h p g ` h k y ~ ` r ~ l f a r $

i z a x i l e & } k ` r ` a w x k ` x k i r h i f f p w x r a x ` w x k ` r ` g l s ` r y l c x k ` M A B A e r ` w ~ l e w ` w a c x ` r x k ` } a w k $ l p x l cb i g p g p f f ie ` + B ) H G E g ` f fw ~ l w w ` w w M A B A B r ` g ` ~ x l r w W a d ` g ` ff a e h i h ` e x i g a f g l e h i x i l e w a w i e A

A ~ ~ f i g a x i l e l c b a g f l c ` e +1 > > ' D ) i e h p g ` h a e k y ~ ` r ~ l f a r i z a x il e } i x k l p x a e a ~ ~ a r ` e x g k a e m ` i e i e ~ p x

r ` w i w x a e g ` + p ~ ~ ` r x r a g ` & f ` c x ) X k i w ` c c ` g x } a w ~ a r x i a f fy a e x a m l e i z ` h b y x k ` M A B A B r ` g ` ~ x l r a e x a m l e i w x &

G M ^ 7 0 78 ; a x a g l e g ` e x r a x i l e l c f d D + p ~ ~ ` r x r a g ` & r i m k x ) X k ` w x a x i w xi g a f r` w p f x w a r ` w k l } e i e x k `k i w x l m r a d

Page 7: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 7/14

Gl rxigle pgf`a r ~rlj` gxile w ie g`r`b`ffar wfig` gpfxpr`w =>0

a g g l d ~ a e i ` h b y a g l e g l d i x a e x h ` g r ` a w ` i e i e ~ p x

r ` w i w x a e g ` l c 0 > 7 / 1 1 % & a e h x k ` r ` s ` r w a f ~ l x ` e x i a f

l c x k ` r` w ~ l e w ` } a w $ ; ; / 8 = d S + h a x a e l x w k l} e )

A ~ ~ f i g a x i l e l c xk ` M A B A m r ` g ` ~ x l r a e x a m l e i w x & b i g p $

g p f fi e ` + 8 > ' D ) r ` h p g ` h x k ` d p w g i d l f $ i e h p g ` h k y ~ ` r $

~ l f a r i z a x i l e + C i m 7 A & p ~ ~ ` r x r a g ` & ri m k x ) b y =8 / ; %

+ C i m 7 A & k i w x l m r a d ) & a e h x k ` i e ~ p x r ` w i w x a e g ` b y

2 0 2 / ; 8 % A c x ` r } a w k $ l p x l c b i g p g p ff i e ` & xk `d p w g i d l f $ i e h p g ` h k y ~ ` r ~ l f a r i z a x i l e r ` g l s ` r ` h xl

; = 1 / 1 0 9 % l c g l e x r l f s a f p ` w + C i m 7 A & k i w x l m r a d )

B a g f l c ` e +1 > a e h 1 > > ,u D & 2 r a i e ) } ` a o f y a e h r ` $

s ` r w i b fy k y ~ ` r ~ l f a r i z ` h H G E e ` p r l e w & } i x k l p x ~ r l $

h p g i e m h ` x ` g x a b f ` g k a e m ` w i e i e ~ p x r ` w i wx a e g `

+ C i m 7 B & p ~ ~ ` r x r a g ` & f ` cx ) X k ` a d ~ f i x p h ` l c x k `

b a g f l c ` e $ i e h p g ` h k y ~ ` r ~ l f a r i z a x i l e } a w r a x k ` r w x a b f `

c l r d ` d b r a e ` ~ l x ` e x i a f w r a e m ie m c rl d $ = 0 x l

$ 0 > d S + D a e e $ ] k i x e ` y x `w x : ^ 4 > = 3 a e h > 7 ; c l r

f > a e h 1 > > ,u D l c b a g f l c ` e & r ` w ~ ` gx i s` f y ) A x 1 > ' D &

b a g f l c ` e g a p w ` h a e k y ~ ` r ~ l f a r i z a x i l e l c 7 3 \ 1 8 d S

+ e 4 2 8 ) & } k i f ` I> > ' D k y ~ ` r ~ l f a r i z ` h H G E e ` p r l e w

b y 8 9 / 2 d S + e $ $ 2 7 ) A ~ ~ f i g a x i l e l c x k ` g l d ~ ` x i $x is ` M A B A B r ` g ` ~ x l r a e x a m l e i w x & G M ^ 7 07 8; & 8> p w ` h

a x a g l e g ` e x r a x i l e l c 1 d D h ` g r ` a w ` h x k ` a d ~ f i x p h `

l c x k ` r `w ~ l e w ` i e h p g ` h b y 1 > > , I D b a g f l c ` e + C im 7 B &

p ~ ~ ` r x ra g ` & r im k x ) b y 3 3 / 2 1 % + e 4 3 ? C i m 7 B &

k i w xl m r a d ) A c x ` r } a w k $ l p x l c x k ` M A B A a r ` g ` ~ x l r

a e x a m l e i w x & a ~ a r x i a f r ` g l s ` r y l c xk ` b a g f l c ` e $

d ` h i a x ` h r ` w ~ l e w ` x l ; ; \/ 1 7 % l c x k ` g l e x r l f + e 4 0 )

} a w l b w ` r s ` h + C i m 7 B & k i w x l m r a d )

X a o ` e x l m ` x k ` r& l p r r ` w p fx w h ` d l e w x r a x ` x k a x H G Ee ` p r l e w ` { ~ r ` w w b l x k M A B A o a e h M A B A B r ` g ` ~ xl r w

Xy~`w l c M ABA`rmig r`g`~xl rw ieslfs`h ie xk`

iekibixlry wyea~xig r`w~lew`w

I e l r h ` r x l w x p h y i e k i b i x l r y w y e a ~ x i g ~ l x ` e x i a fw i e

i w l f a x il e & g p f x p r `w } ` r ` g l e x i e p l p w f y ~ ` r c p w ` h } i x k a

w l f p x i l e g l e x a i e i e m G E Q [ + 2 > , u D ) a e h H $ A ^ S

+ 8> ,u D ) & a e h a d l e l ~ l f a r w x i d p f a x i l e ` f ` g x r l h ` } a w

~ f a g ` h h i r ` g x fy ie x k ` ^ G f a y ` r @ s ` r y w x i d p f a x i l e

` s l o ` h a e I ^ W ^ } i x k l p x c a i f p r ` & a e h } i x k a g l e w x a e x

h ` f a y X k ` a d ~ f i x p h ` l c x k ` I^ W ^ w ` s l o ` h i e H G E

e ` p r l e w } a w m r a h ` h a e h h ` ~ ` e h ` h l e x k ` w x r ` e m x k l c

w x i d p f a x i l e + C i m 8 A ) I e a m i s ` e g ` f f a e h c l r ae l e $ w a x p r a x i e m w x i d p f p w & x k ` a d ~ f i x p h ` l c I ^ W ^ w

A

8 > ~A

3> ~u

; > ~ A

1 > > ~ AIx

0 d S

1 1 > d w

@ f

u - u u u i d

1 > d w

Glexrlf

Bigpgpf f ie` 8> ~ D

R`gls`r y

G

u $ = >}g

1%3l

$ 1 1 >i$ 17>

H

0

2> dw

m

"3

@6

I>

• Af fll

e d d l

H

>

hf

• l

_T

$12> $11> $1>> $9 > $; > $= > $3> $0>D` dbra e` ~l x` e xia f+dS)

Cim 8 I^W^w iehpg`h ie HG E g`ffw by xk` wx idpfaxile lc ^proiej` g`f fw +A) Ie xk` ~r`w`eg` lc xk` @A Ar`g`~xlr aexamle iwxw & c i`fh wx idpfaxile lc ^Gw iehpg`h mrah`h d lelwyea ~xig I^W^w +B) Xk` I^W ^ }aw cpf fyaeh r`s`rwibfy abl fiwk`h by xraewi`ex a~~figa x ile lc bigpgpff ie` +8> uD ) +G) Gprr`e x iej`gx ilew fawx iem10> dw }`r` pw`h xl `safpax` @i~w~ Xk` ad~ fi xph` lc xk` I^W^ } aw r`hpg`h& aeh xk` ~l fari xy ies`rx`h }ixkiegr`awiem ky~ `r~lfarizax ile +H) R`fax ilewki~ b`x}``e xk` d`dbrae` ~ lx`exiaf aeh I^ W^ ad ~fi xph` aw

r`glrh`h ie clpr HG E g`ffw @x~w~ }aw $; 9 \/ = dS

Page 8: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 8/14

=>3 H% D lpmie lx aeh B% K% Muik}i f r

w k l } ` h f i x x f ` s a r i a b i f i x y & b p x h i c c ` r ` h g l e w i h ` r a b f y

c r l d g ` f f x l g` f f % C l r 1 7 g ` f f w x ` w x ` h & x k ` I ^ W ^

a d ~ f i x p h ` a s ` r a m ` h = % 8 /\\ 7 % 2 d S & a e h x k ` d ` a e I ^ W ^

h p r a x i l e } a w 8 2 % 7 \ 7 % = d w % I ^ W ^ w } ` r ` c p f f y bf l¢ go `h

b y a ~ ~ f i g a x i l e l c b i g p g p f f i e ` + 8 > ' D ) a e h r ` g l s ` r ` h

g l d ~ f ` x ` f y a c x ` r } a w k $ l p x l c x k ` h r p m + C i m % 8B ) %

L e ` g l r l f f a r y l c x k i w c ie h i e m i w x k a x x k ` r ` s ` r w a f

~ l x ` e x i a f l c x k ` i e k i b i x l r y ~ l x ` e x i a f w + @ T~ w ~) l p m k x

x l b ` g f l w ` x l x k ` ` q p i f i b r i p d ~ l x ` e x i a f c l r G I

i l e w % X l x ` w x x k i w k y ~ l x k ` w i w & } ` ` { a d i e ` h x k ` a d ~ f i $

x p h ` l c ` s l o ` h I ^ W ^ w } k i g k } ` r ` w p ~ ` r id ~ l w ` h l e

k y ~ ` r ~ l f a r i z i e m s l f x a m ` w x` ~ w % X k ` a d ~ f i x p h ` l c x k `

C4> %>3 Kz

"xF,c

8 d S

I 1> dw

C 4 > % 2 K z8 d S

I 1> dw

C 4 0 K z

8 d S

I1> dw

8 d S

I 0 > d w

C 4 0 > K z

8 d S

o 1 > d w

Cim % 0 % X k ` ad~ f i xp h ` l c `s l o `h I^W^w wk l }w f i x x f` c r `q p `e gy h `~ `e h `e g` % Xk ` g` f f }aw da ie xa ie `h a x$ 0> dS aeh r`~`xi x is` w x idpfaxile l c ^G fay`r }aw h`fis`r`h a x h icc`r`ex cr`qp`egi`w +> %>3$0> K z)% Elx`xkax ie xk iw `{ad~ f` aeh clr `s`ry cr`qp`egy x`w x`h & xk`r` }aw el r`hpgxile ie xk` ad~ fi xph` l c xk` w`glehI^W^ g l d~ ar`h }i xk xk ` c ir wx % Cl r 0 Kz w x idp fa x il e & x}l I^W^ w + w xa rw ) a r` h ra} e b `e `a xk a x xk ` wad` x id`wgaf` a w xk l w` ab l s ` % Xk ` ab wl fp x` ad~ f i xp h ` l c `s l o `h I^W^w wk l }`h }`ao h `~ r` w w il e h p r ie m 0 > Kz

wxidpfaxile %

Page 9: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 9/14

Glrxiglepgf`ar ~rlj`gxilew ie g`r`b`ffar wfig` gpfxpr`w =>=

I^W^ h`gr`aw`h }ixk iegr`awiem ky~`r~lfarizaxile&

bpx r`s`rwaf l c I^W^ ~ lfar ixy + $ ; 9 \\\= dS) }aw

lbw`rs`h ie lefy clpr lpx lc 17 g`ffw x`wx`h

+Cim 8G& H)

Lpr r`wpfxw h`dlewxrax` xkax ie g`r`b`ffar wfig`

gpfxpr`w& wxidpfaxile lc ^Gw iehpg`w dlelwyea~xig

I^W^w }kigk ar` d`hiax`h by xk` agxisaxile lc

MABAA r`g`~xlrw

Cr`qp`egy$h`~`eh`eg` lc iekibixlry ~lwxwyea~xig

~lx`exiafw `slo`h ie h``~ g`r`b`ffar epgf`i e`prlew

Xl x`wx xk` cr` qp`egy$h`~`eh`eg` lc slo`h I^W^w&

^Gw }`r` wx idpfax`h }ixk r`~`xixis` wxidpfi ax hicc`r$

`ex cr`qp`egi`w +>>33 / 0> Kz) @s`ry wxidpfpw xrim$

m`r`h ae I^W^ }ixklpx caifpr` +Cim 0)& aeh lefy

r`faxis`fy wdaff axx`epa xile w lc I^W^ ad~fixph`w }`r`

w``e Clr wxidpf axil e cr`qp`egi`w lc 777 aeh 0 Kz&

xk` w`gleh I^W^ }aw r`hpg`h by ;3 \/ 37% +e 4 8)&

aeh by 8; / 31% +e 4 9)& r`w~`gxis`fy& gld~ar`h

}ixk xk` ad~fixph` lc xk` cirwx `slo`h I^W^ Ax a

kimk`r wxidpfaxile rax` +0> Kz)& xk`r` }aw x`d~lrafwpdda xil e lc I^W^w& kl}`s`r xk` abwlfpx` ad~fix ph`

lc xk` w`gleh I^W^ }aw r`hpg`h by lefy 11 / 210%

+e 4 12) gld~ar`h }ixk xk` ad~fixp h` lc xk` cirwx

I^W^ @s`e }ixk a xraie lc 1> ~pfw`w `figix`h ax 0> Kz&

xk` ad~fi xph` lc xk` fawx I^W^ }aw lef y h`~r`ww`h by

2> \ 28% +e 4 12)& gld~ar`h }ixk xk` ad~fix ph` lc

xk` cirwx `slo`h I^W^ Ie ahhixile& I^W^w `slo`h by

kimk$cr`qp`egy wx idpfaxi le +0> Kz) }`r` wp~`rid$

~lw`h le a wpwxaie`h ky~`r~lfarizaxile +Cim 0& blx$xld xrag`) ^`rcpwile lc GM^ 7078; +1 dD) kah el

`cc`gx le `ixk`r xkiw ky~`r~lfarizaxile& lr xk` ad~fi$

xph` lc `slo`h I^W^w +e 4 0& haxa elx wkl}e)

Xao`e xlm`xk`r& xk`w` r`wpfxw iehigax` xkax I^W^w

`slo`h by wx idpfaxi le lc ^Gw wkl} fixxf` cr`qp`egy$

h`~`eh`eg` ie HGE e`prlew Cprxk`rdlr`& `s`e ax

kimk wxidpfaxile rax`w& MABA r`f`aw`h crld ^G

a{le x`rdieafw hl`w elx a~~`ar xl agxisax` MABAB

r`g`~xlrw

@cc`gx l c `slo`h iekibixlry ~lwxwyea~xig ~lx`exiafw le

xleig ciriem lc h``~ g`r`b`ffar epgf`i e`prlew

Xraewi`ex h`~lfarizaxile lc HGE e`prlew `figix`hagxile ~lx`exiafw& }klw` cr`qp`egy h`~`eh`h le xk`

A7 > K z 3 > K z

u $2>

i

i- 0 d w u

>%8 eA I % & & % J - T

ll I $ xz

\ %i F u

a d w u ` u1 2 K z

m

iv

$0 0

$ = 0 u IAAaf

2 > > d w 2 > K z

G 31$u

&G ¾

v

= 0 1 > K zI v i v i I I v i i f f f v A

: w 0 K z ; % 3 3 K z0 > > d w

Cim 3 Dlhpfaxile lc HGE hiwgkarm`w by `slo`h I^W^w +A) Ie a HGE e`prle& gprr`ex }aw iej`gx`h xliehpg` hiwgkarm` rax`w lc 7>& 3> aeh 9> Kz A wiemf` I^W^ +arrl} k`ah) slo`h by xk` wxidpfaxile lc Gwhpriem xk` h`~lfariziem ~pfw` xraewi`exfy wxl~~`h agxile ~lx`exiaf hiwgkarm`w +B) Dlhpfaxile lc hicc`r`exrax`w lc ciriem +0& ; aeh 12 Kz) by cis` I W^w slo`h ax a glewxaex cr`qp`egy lc 2> Kz Ax s`ry cr`qp`egyx`wx`h& xraiew lc I^W^w iehpg`h a ky~`r~lfarizaxile xkax xraewi`exfy wxl~~`h xk` agxile ~lx`exiaf hiwgkarm`+G) Dlhpfaxile lc glewxaex ciriem +3 Kz) by I^W^w `slo`h ax hicc`r`ex cr`qp`egi`w +0& 333 aeh 1> Kz)Elx` xkax a fl} cr`qp`egy +0 Kz) lc I^W^w glpfh wfimkxfy wfl} hl}e agxile ~lx`exiaf hiwgkarm`w +f`cx xrag`)&}k`r`aw I^W^w `figix`h ax kimk`r cr`qp`egi`w ~arxiaffy +333Kz) lr gld~f`x`fy +1>Kz) ablfiwk`h xk`

m`e`raxile lc agxile ~lx`exiafw

Page 10: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 10/14

=> ; H% Dlpmielx aeh B% K% Muik}if`r

a d ~ f i x p h ` l c x k ` h ` ~ l f a r i z i e m ~ p fw ` + C i m % 3 A ) % A

w i e mf ` I ^ W ^ ` s l o ` h b y ^ G w x i d p f a x i l e } a w g a ~ a b f ` l c

x r a e w i ` e x f y i e x ` r r p ~ x i e m x k i w c i r i e m & ` s ` e a x k i m k r a x ` w

l c h i w g k a r m ` + C i m % 3 A ) % B ` g a p w ` x k ` c r ` q p ` e g y $

h ` ~ ` e h ` e g ` l c ` s l o ` h I ^ W ^ w } a w e l x ~ r l e l p e g ` h & } `

x ` w x ` h x k ` ` c c` g x l c a w k l r x x r a i e l c I ^ W ^ w l e x l e i g

a g x i l e ~ l x ` e x i a f h i w g k a r m ` w % I e a c i r w x w ` x l c ` { ~ ` r $

i d ` e x w & x r a i e w l c ci s ` I ^ W ^ w + 2 > K z ) } ` r ` h ` f i s ` r ` h} k i f ` x k ` H G E g ` f f w c i r ` h a x r a x ` w l c 0 & ; a e h 1 2 K z

+ C i m % 3 B ) % I r r ` w ~ ` g x i s ` l c x k ` c i r i e m r a x` w & x k ` x r a i e w

l c I ^ W ^ w k y ~ ` r ~ l fa r i z ` h x k ` H G E e ` p r l e w a e h

x r a e w i ` e x f y ~ r ` s ` e x ` h m ` e ` r a x i l e l c a g x i l e ~ l x ` e x i a f w %

I e a w ` g l e h w ` x l c ` { ~ ` r i d ` e x w & } ` x ` w x ` h x k `

i e cf p ` e g` l c x k ` i e x ` r x r a i e c r ` q p ` e g y i e d l h p f a x i e m

x l e i g c i r i e m a x 3 K z % C i s ` I ^ W ^ w } ` r ` x r i m m ` r ` h a x

h i c c ` r ` e x cr ` q p ` e g i ` w + 0 & 3 %3 3 a e h 1 > K z ) % A x 0 K z & x k `

c i ri e m c r ` q p ` e g y l c H G E g ` ff w } a w w f i mk x f y h ` g r ` a w ` h

+ C i m % 3 G & f ` c x ~ a e ` f ) & a e h a x k i m k ` r I ^ W ^ c r ` q p ` e g i ` w &

a g x i l e ~ l x ` e x i a f h i w g k a r m ` w } ` r ` ~ a r x i a f f y + 3 %3 3 K z ) &

l r g l d ~ f ` x ` f y + 1 > K z ) a b l f i w k ` h + C i m % 3 G & d i h h f ` a e h

r i m k x ~ a e ` f w ) %

X a o ` e x l m ` x k ` r & l p r h a x a w p m m ` wx x k a x a w i e m f ` I ^ W ^

` s l o ` h b y x k ` w x i d p f a x i l e l c ^ G w g a e x r a e w i ` e x fy a f x ` r

x k ` h iw g k a r m ` l c H G E e ` p r l e w & a e h x k a x x r a i e w l c

` s l o ` h I ^ W ^ w g a e d l h p f a x ` i e x r i e w i g h i w g k a r m `

~ a x x ` r e w i e x k ` w ` e ` p r l e w %

Xleig iekibixile l c h``~ g`r`b` ffar epgf`i e`prlew b y

^proiej` g`ffw

W i m e i ci g a e x e p d b ` r w l c w ~ l e x a e ` l p w I ^ W ^ w } ` r `

l b w ` r s ` h i e H G E g ` ff w i e l r m a e l x y ~ i g g ` r ` b ` f f a r

g p f x p r` w & ` s ` e i e x k ` ~ r ` w` e g ` l c @ A A r ` g ` ~ x l r a e x a m $

l e i wx w % P e h ` r x k ` w` g l e h i x i l e w & x k ` c i r ie m r a x` l c H G E

g ` f fw } a w 1 2 / 7 K z + e 4 1 7 )% H ` ~ l f a r i z a x i l e l c H G E

g ` f fw b y w p w x a i e ` h i e j ` g x i l e l c g p r r ` e x i e g r ` a w ` h x k `

c i ri e m c r ` q p ` e gy x l a d a { i d a f r a x ` l c 7 3 $ I $ 0 K z

+ e 4 1 7 ) & a e h x k ` i e w x a e x a e ` l p w c i r i e m w k l } ` h a } i h `

r a e m ` l c i e x ` r w ~ io ` i e x ` r s a fw + C i m % = A ) % A ~ ~ f i g a x i l e l c

b i g p g p ff i e ` + 8 > ' D ) a b l f i w k ` h w ~ l e x a e ` l p w I ^ W ^ w

a e h r ` w p f x ` h i e a r ` m p f a r c i r ie m ~ a x x ` r e + C i m % = B ) &

` w x a b f iw k i e m x k a x w ~ l e x a e ` l p w I ^ W ^ w } ` r ` r ` w ~ l e w i b f `c l r x k ` i r r ` m p f a r ~ a x x ` r e l c a g x i l e ~ l x ` e x i a f h i w g k ar m ` %

\u GEQ[2> ~D& H$A^S8> ~D

I2> dS

K^ : $70 dS J

11>> dw

J%

> %2

>% 1

>> 1 > 2 > 7 > 8 > 0 >

C r` q p ` e g y +Kz)

GEQ[ 2> ~D& H$A^S8> ~D& BIGPGPFFIE@8> ~D

> %2

i

>%1

2> dS >K^: $7 0 d S J f

1>> dw

1> 2 > 7 > 8>

C r` q p ` e g y +Kz)

C i m % = % D l h p f a x i l e l c x k ` i e x r i e w ig a gx i si x y l c H G E e ` p r l e w b y w ~ l e x a e ` l p w I ^ W ^ w % + A ) I e x k ` ~ r ` w ` e g `l c @ A A r ` g ` ~ x l r a e x a m l e i w x w + G E Q [ & 2 > , x D ? H $ A ^ S & 8 > , I D ) & a H G E e ` p r l e c i r` h a x k i m k c r ` q p ` e gy+ 8 > K z ) h p r i e m i e j ` g x il e l c a w p w x a i e `h h ` ~ l f a r i z ie m g p r r ` e x _ K l f h i e m ~ l x ` e x i a f + K ^ ) : $ 7 0 d S T % W ~ l e $x ae `l p w I^W^w +a r rl } k ` ah ) x rae w i `e x f y h i w rp ~ x `h x k ` ag x i l e ~ l x `e x i a f h i wgk a rm `w & x k p w i e g r`a w i e m xk ` rae m `l c i e w x ae x ae `l p w c r`q p `e g i ` w a w i f f p w x ra x `h i e x k ` k i w x l m rad l e x k ` r i m k x % +B ) Wad` g` f f a w i e A % ^` r cp w i l el c x k ` gp f x p r` } i x k a wl f p x i l e gl e x a i e i e m b i gp gp f f i e ` +8 > , J D) ab l f i wk `h w~ l e x a e `l p w I^W^w ae h r` wp f x `hi e a dl r` r`m p f a r ~ a x x ` re l c h i wgk a rm `w aw wk l } e b y x k ` e a r rl } `r c r`q p `e gy h i w x r i b p x i l e + r i m k x) % B`gap w`b i gp gp f f i e ` a f wl h `~ l f a r i z`h x k ` g` ff & x k ` d`d b rae ` ~ l x `e x i a f } aw ah j p w x `h x l x k ` wad` s a f p ` a w i e xk `glexrl f glehix ile +K^: $7 0 dS )% Clr xk` kiwxlmradw& ~rlba bif i xy }aw gafgpfax`h aw xk` epdb`r lc `s`exw

lggprriem ie a mis`e cr`qp`egy raem` +biew 4 1 Kz) hisih`h by xk` xlxa f epdb`r lc `s`exw%

0 >

Page 11: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 11/14

Glrxig lepg f`ar ~rlj`gxilew ie g`r`b`ffar w f ig` gpfxpr`w =>9

W ~ l e x a e ` l p w I ^ W ^ w a r ` f io ` f y x l i e cf p ` e g ` x k `

` { g i x a b i f ix y l c H G E e ` p r l e w % X l x ` w x x k iw k y ~ l x k ` w i w &

r ` g l r h i e m w } ` r ` g a r r i ` h l p x i e g p f x p r ` w } k i g k } ` r `

g l e x i e p l p w f y ~ ` r c p w ` h } i x k a X y r l h ` w l f p x i l e

g l e x a i e i e m b i g p g p f f i e ` + 8 > ' D ) % P e h ` r x k ` w ` g l e $

h i x i l e w & H G E e ` p r l e w w ~ l e x a e ` l p w f y h i w ~ f a y ` h

b p r w x w l c a g x i l e ~ l x ` e x i a f w w ` ~ a r a x ` h b y a ~ r l e l p e g ` h

a c x ` r k y ~ ` r ~ l f a r i z a x i l e + C i m % ;A ) % H p r i e m x k ` c i rw x

d i e p x ` w l c a ~ ~ f i g a x i l e l c b i g p g p f f ie ` & b p r w x w l g g p r r ` h

a x a c r ` q p ` e g y l c > % 8 \/ > %1 K z & b ` c l r ` w x a b i f i z i e m a x a

c r ` q p ` e g y l c > %> 3\ \\ > % > 7 K z + e 4 =) % X k ` b p r w x i e m

a g x i s i x y } a w ~ r l b a b f y h p ` x l x k ` ~ r ` w ` e g ` l c

A Xyrlh ` wlfpx ile

1> dS

_ \0>> dw

Bigpgpffie`&8 > ~D

GEQ[ 2> ~D& H$A^S8 > ~D

Bigp`pffie`&8> ~D

G 1 $ r { > %0 ~D

Bigpgpffie` 8> ~D

/

f f f f f/

I f f f I I 1 & 1 & I & 1 1 1 1 1 1 1 1 11 1I I I I I I I I I I I

• % 4

I C I I f

0 d S

8> dw

Cim % ; % ^Gw `{`rx a xleig glexrl f le H GE e`prlew% +A) ^`rcpwile lc xk` gpfxpr` }ixk Xyrlh` wlfp xileglexaie iem b igpgpff ie` +8> ' D) iehpg`h rkyxkdig bprwxw lc ag x ile ~lx`exia fw ie a HGE e`prle% +B) Ie xk`~r`w`eg` lc GE Q[ +2> ' D) ae h H $A^S +8>,u D)& x rae wi`ex a~~ figax ile l c b igpgpff ie` h`~lfariz`h xk` g`ffcrld xk` lrim ieaf klfh iem ~lx`exia f lc $ 3 > dS & r`wpfxiem ie wpwxa ie`h agxile ~lx`exia f h iwgkarm`% E lx`xk a x xk ` ad~ f i xp h ` l c w~io`w a r` x rp e ga x`h % +G) Ie xk ` ~ r`w`e g l c XX[ +> %0 ' D) & HG E e `p rl e w h i w~ fay`hXX[ $r` w i w xae x b ago m rl p e h e l iw` xk a x }aw d aro `h fy r`h p g`h b y a x r ae w i`e x a~ ~ f iga x il e l c b igp gp ff ie `+8> 'D ) wpmm`wxiem xkax xk` eliw` r`wpfxw crld agxile ~l x`exia f$ ieh`~ `eh`ex d ieia xpr` I^W^w% A w f imkx

h`~lfarizax ile lc xk` g`f f aggld~aei`h xk` r`hpgxile lc xk` eliw` %

Page 12: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 12/14

=1> H% Dlpmielx aeh B% K% Mu%k}if`r

a { l e g l f f a x ` r a f w c r l d H G E g ` ff w & } k i g k k a h b ` ` e

h ` d l e w x r a x ` h x l ` { i w x i e x k i w ~ r ` ~ a r a x i l e % 8

I e x k ` ~ r` w ` e g ` l c G E Q [ a e h H $ A ^ S + 2 > a e h

8 > ' D & r ` w ~ ` g xi s ` fy ) & x ra e w i ` e x a ~ ~ f i g a x i l e l c b i g p $

g p f fi e ` + 8 > , u D ) h ` ~ l f a r i z ` h H G E e ` p r l e w b y

8 \ 1 %0 d S i e 2 1 l p x 2 3 g ` f f w x ` w x ` h & a e h i e h p g ` h

w p w x a i e ` h a g x i l e ~ l x ` e x i a f h i w g k a r m ` w + C i m % ; B ) %

D i e i a x p r ` I ^ W ^ w g l p f h g f ` a r fy b ` r ` w l f s ` h ie w i { l p xl c 1 7 g ` f f w r ` g l r h ` h i e w l f p x i l e g l e x a i e i e m X X [

+ > %0, u D ) % P e h ` r x k ` w ` g l e h i x i l e w & b i g p g p f f i e `

h ` ~ l f a r i z ` h H G E g ` f f w b y 8 %0 \ 1 %= d S + e 4 1 7 )& a e

` c c` g x x k a x } a w a g g l d ~ a e i ` h b y a h ` g r ` a w ` l c b a g o $

m r l p e h e l i w ` + C i m % ; G ) a e h } k i g k i e h i g a x ` w x k a x ` s ` e

d i e i a x p r ` I ^ W ^ w d a r o ` h f y i e c f p ` e g ` x k ` d ` d b r a e `

~ l x ` e x ia f l c H G E e ` p r l ew %

X k ` r l f ` l c M A B A B r ` g ` ~ x l r w i e H G E w y e a ~ x i g

x r a e w d i w w i l e i w e l x g f ` a r % B a g f l c ` e l e f y } ` a o f y

k y ~ ` r ~ l f a r i z ` h H G E g ` ff w & a e h G M ^ 7 07 8; e ` i x k ` r

a c c `g x ` h xk ` w p w x a i e ` h k y ~ ` r ~ l f a r i z a x i l e i e h p g ` h b y

k i m k c r ` q p ` e g y w x i d p f a x i l e w & e l r x k ` a d ~ f i x p h ` l r

h p r a x i l e l c i e h i s i h p a f I^ W ^ w % I e m ` e ` r a f& x k ` h ` e w i x y

l c M A B A B r ` g ` ~ x l r w i e r l h ` e x g ` r ` b ` f f p d a ~ ~ ` a r w x l

b ` q p i x ` f l } & a w h ` x ` r d i e ` h b y q p a e x i x a x i s ` r ` g ` ~ x l ra p x l r a h i l m r a ~ k y %89 X k ` w ` a p x k l r w k a s ` r ` ~ l r x ` h a

x r a e w i ` e x ` { ~ r ` w w i l e l c k i m k f ` s` f w l c M A B A B b i e h i e m

w i x ` w i e x k ` H G E & ~ ` a o i e m a x ~ l w x e a x a f h a y 7 a e h

h ` g r ` a w i e m x l } ` a o f ` s ` fw a w w ` w w ` h a x ~ l w x e a x a f h a y

2 1% A e l x k ` r a p x l r a h i l m r a ~ k i g w x p h y r ` ~ l r x ` h x k `

a b w ` e g ` l c b i e h i e m w i x` w c l r M A B A B r ` g ` ~ x l r w i e

k p d a e g ` r ` b ` f f a r h ` e x a x ` e p g f ` p w ,

HIWGPWWILE

] ` k a s ` g k a r a g x ` r i z ` h xk ` ~ r l ~ ` r x i ` w l c a d a j l r

g ` r ` b ` f f a r ~ r l j ` g x i l e & c r l d ^ p r o i e j ` g ` f f w xl h ` ` ~

g ` r ` b ` f f a r e p g f ` i & i e a e ie sixrl ~ r ` ~ a r a x i l e % X k i w

~ a x k } a y r ` ~ r` w ` e xw x k ` d l w x i d ~ l r x a e x l p x ~ p x c r l d

x k ` g ` r ` b ` f f a r g l r x ` { & a e h i w h i w x i e m p i w k ` h b y i x w

i e k i b i x l r y e a x p r ` % ] ` k a s ` w x p h i ` h x k ` i e x r i e w i g c i ri e m

~ a x x ` r e l c e ` p r l e w i e x k ` H G E a e h g k a r a g x ` r i z ` h xk `

i e k i b i x l r y g l e x r l f l c x k ` i r a g x i s i x y b y ^ G w %

^ p r o i e j ` g ` f f $ i e h p g ` h i e k i b i x l r y ~ l x ` e x i a f w i e h ` ` ~

g ` r ` b ` f f a r e p g f ` i e ` p r l e w

A f x k l p m k H G E e ` p r l e w ~ l ww ` ww b l xk M A B A A a e h

M A B A a r ` g `~ x l rw & } ` h ` d l e w x r a x ` x k a x l e f y M A B A A

r ` g ` ~ x l r w a r ` i e s l f s ` h i e x k ` ^ G $ i e h p g ` h i e k i b i x l r y

~ l x ` e x i a f w & g l e c i r d i e m ~ r ` s i l p w l b w ` r s a x i l e w % 78-02

K l } ` s ` r & x k ` d ` a e @ i ~ w ~ } a w $ ; 9 / = d S & a e h x k `

r ` s ` r w a f ~ l x ` e x i a f c l r d p w g i d l f $ i e h p g ` h r ` w ~ l e w ` w

} a w $ ; ; \ 8 % = d S % X k ` w ` s a fp ` w a r ` d l r ` k y ~ ` r $

~ l f a r i z ` h x k a e x k ` x k ` l r ` x i g a f s a f p ` l c @ l + $ = 3 d S ) &

a w w p d i e m a e i e x r a g ` f f p f a r g k f l r i h ` g l e g ` e x r a x i l e l c

; d D % 72 m f ^W^ s a fp ` w r a e m ie m c r l d $ = 0 x l $ 3 3 d S

k a s ` b ` ` e r ` ~ l r x ` h c l r k i ~ ~ l g a d ~ a f a e h e ` l g l r x i g a f

~y rad iha f g` f f w % 1¾-83-8= Xkiw h i c c` r`eg` glp fh ieh ig a x`

` i x k ` r x k a x @ u~ w~ i e H G E e ` p r l e w i w d l r ` k y ~ ` r ~ l f a r $

i z ` h xk a e i e l x k ` r r ` m i l e w l c x k ` G E W & w p m m ` w xi e m x k a x

H G E e ` p r l e w d a i e x a i e a w p r ~ r i wi e m f y f l } g l e g ` e $

x r a x i l e l c i e x r a g ` f f p fa r g k f l r i h ` +8 % 3 d D ) & l r x k a x ^ G w

i e w f ig ` g p f x p r ` w c l r d a x f ` a w x ~ a r x l c x k ` i r w y e a ~ w ` w l e

` f ` g x r l x l e i g a f fy r ` d l x ` h ` e h r i x ` w l c H G E e ` p r l e w %

^ r ` f i d i e a r y d l r ~ k l f l m i g a f h a x a l b x a i e ` h } i x k f im k x $d i g r l w g l ~ y f ` e h w p ~ ~ l r x x l x k ` f a x x ` r k y ~ l x k ` w i w w i eg `

a ~ l r x i l e l c i e k i b i x l r y w y e a ~ w` w l c H G E e ` p r l e w i e

g ` r ` b ` f f a r w f i g ` g p f x p r ` w a ~ ~ ` a r x l b ` a { l h ` e h r i x i g

+ C i m % 1 )% ^ r ` s i l p w d l r ~ k l f l m i g a f w x p h i ` w ~ ` r c l r d ` h i e

x k ` g a x 2-81 a e h i e x k ` d l e o ` y ; w ` ` d a f w l x l i e h i g a x `

x k ` ~ r ` w ` e g` l c a { l h ` e h r i x i g a e h a { l w l d a x i g w y e a ~ w ` w

l e H G E e ` p rl e w % C p r x k ` r d l r ` & x k ` ~ l f a r ix y l c

` s l o ` h I ^ W ^ w g l p f h e l x b ` r ` s ` r w ` h } k ` e w k a r ~

` f ` g x r l h ` w } ` r ` ci f f `h } i x k a 2 D O G 1 w l f p x i l e

+ p e ~ p b f i w k ` h l b w ` r s a x i l e ) & x k p w w p ~ ~ l r x i e m x k `

k y ~ l x k ` w i w l c a h ` e h r i x i g fl g a f i z a x i l e l c ^ G w y e a ~ w ` w

l e H G E e ` p r l e w %

R k y x k d i g a g x il e ~ l x ` e x i a f c i r i e m

I x k aw b ` ` e r` ~ l r x ` h x k a x d a d d a f i a e H G E e ` p r$

l e w ie sisl & u2 aw }`f f aw ie six rl 20-7¾ a r ` w ~ l e x a e ` l p w f y

a g x i s ` & b p x i x } a w e l x g f ` a r } k ` x k ` r x k i w a g x i s i x y i w

` e x i r ` f y w y e a ~ x i g i e l r i m i e l r i e ~ a r x h p ` x l i e x r i e w i g

~ a g ` d a o ` r $ f i o ` a gx i s ix y % L p r r ` w p fx w w k l } x k a x i e x k `

~ r `w ` e g` l c @ A A a e h M A B A A r ` g ` ~ xl r a e x a m l e i wx w &

x k ` d a j l r i x y l c H G E g `f fw h i w ~ fa y ` h w ~ l e x a e `l p w

a g x i l e ~ l x ` e x i a f h i w g k a r m ` w a e h c i r ` h c l r ` { x ` e h ` h

~ ` r i l h w l c x i d ` + w `s ` r a f d i e p x ` w ) } i x k l p x a g g l d d l $

h a x i l e % L p r h a x a w p m m ` w x x k a x H G E e ` p r l e w b ` f l e m

x l x k ` c a d i f y l c w i e m f ` $ g ` ff l w g i f f a x l r w &2; wieg` a b`ax$

i e m $ x y ~ ` a g x i s i x y ~ ` r w i w x w h p r i e m w y e a ~ x i g i w l f a x i l e %

X k ` d a { i d a f c i ri e m cr ` q p ` e g y l c H G E g ` ff w } a w

7 9 / 1 > K z h p r i e m g l e x i e p l p w i e j ` g x i l e l c g p r r ` e x %

K l } ` s ` r & x k ` w ` e ` p r l e w g a e c i r ` a x c r ` q p ` e g i ` w p ~ x l

1 0> K z } k ` e x k ` y a r ` h ` ~ l f a r i z ` h b y b r i ` c h ` ~ l f a r $

i z ie m g p r r` e x ~ p f w ` w + H % D l p m i e l x & p e ~ p b f i w k ` h

l b w ` r s a x i l e ? w ` ` a f w l R ` c % 7 0 ) & l r h p r i e m x k ` b i g p $

g p f f i e ` $ i e h p g ` h b p r w x i e m a g x i s i x y + p ~ x l 2 0 > K z ) %

X k i w c i eh i e m i e h i g a x ` w x k a x H G E e ` p r l e w i e l r m a e $

l x y ~ i g g ` r ` b ` f f a r g p f x p r` w w k a r ` w i d i f a r ` f ` g x rl ~ k y w i l $

f l m i g a f g k a r a g x ` r i w x i g w } i x k H G E g ` f fw i e a g p x `

g ` r ` b ` f f a r w f i g ` w %20 X k ` r k y x k d i g c i r i e m l b w ` r s ` h i e

H G E e ` p r l e w h i c c ` rw & k l } ` s ` r & c r l d x k a x r ` ~ l r x ` h

c l r x k a f a d p w e p g f ` i e ` p r l e w ie sixrl } k i g k h i w ~ f a y

x } l x y ~ ` w l c c ir i em d l h ` w & h ` ~ ` e h i e m l e x k ` d ` d $

b r a e ` ~ l x ` e x i a f % 29

I x i w h i c c ig p f x x l o e l } } k ` x k ` r x k ` i e x r i e w i g ~ a g ` $

d a o ` r $ f i o ` a g x i s i x y { i w x w ie wixp } k ` r ` H G E e ` p r l e w

g a e e l x b ` r ` g l r h ` h i e w y e a ~ x i g i w l f a x i l e % A f a r m `

b l h y l c ` { ~ ` r i d ` e x a f ` s i h ` e g` h ` d l e w x r a x ` w & k l } $` s ` r& x k a x H G E a e h H ` i x ` rw e p gf ` i e ` p r l e w a r `

w ~ l e x a e ` l p w f y a g x is ` ie wixp% 12%22 Xk ` h i wg karm ` ~a x x` re

i w p w p a ff y i r r ` m p f a r & } k i g k } l p f h b ` g l d ~ a x i b f ` } i x k

x k ` s i ` } x k a x a e i e x r i e w i g a g x i s i x y i w d l h p f a x ` h b y

w y e a ~ x i g i e ~ p xw % K i m k f y r k y x k d i g h i w g k a r m ` w k a s ` &

k l } ` s ` r & b ` ` e r ` g l r h ` h i e x k a f a d i g r ` f a y g ` ff w i e

g a x w p e h ` r p r ` x k a e ` a e ` w x k ` w i a % 80 I x i w i e x ` r ` w x i e m

x k a x ^ i e a p f x a e h H ` w g k r e ` w 82 } ` r ` a b f ` x l h ` d l e $

w x r a x ` xk a x e ` p r l e w l c x k ` H G E a r ` r ` w ~ l e w ib f ` c l r

i d ~ l w i e m x k i w r k y x k d i g i x y l e x k ` x k a f a d i g x a r m ` x

e ` p r l e w & w p m m ` w xi e m x k a x p e h ` r w p g k ` { ~ ` r i d ` e x a f

g l e h i x i l e w & H G E e ` p r l e w a r ` g a ~ a b f ` l c m ` e ` r a x i e m

Page 13: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 13/14

Glr xiglep gf`ar ~rlj`gxilew ie g`r`b`ffar w f ig` gpfxpr`w =11

k i m k f y r ` m p f a r ~ a x x ` r e w l c a g x is i x y % I e h ` ` h & ~ r ` s i l p w f y

r ` ~ l r x ` h h a x a h ` d l e w x r a x ` x k a x H G E e ` p r l e w ~ l w $

w ` ww x k ` r ` ~ ` r x l i r ` l c s l f x a m ` $ h ` ~ ` e h ` e x g p r r ` e x w r ` $

q p i r ` h c l r m ` e ` r a x i e m ~ a g ` d a o ` r $ f i o ` a g x i s i x y % 23&7¾

G l e x r l f l c h ` ` ~ g ` r ` b ` ff a r e p g f ` i a g x i s i x y b y i e k i b i xl r y

~ l w x w y e a ~ x i g ~ l x ` e x ia f w

X k ` a g x i s a x i l e l c ^ G w a x h i c c ` r ` e x c r ` q p ` e g i ` w + c r l d

> % >3 3 x l 0 > K z ) ~ r l h p g ` h I ^ W ^ w l c a r ` f a x is ` f y gl e $

w x a e x a d ~ f i x p h ` & ` s ` e a x k i m k w x i d p f a x i l e r a x` w % X k i w

c i e h i e m g l e x r a w x w } i x k x k ` ~ r l e l p e g ` h c r ` q p ` e g y $

h ` ~ ` e h ` e x h ` g r ` a w ` w i e h r i s i e m c l r g ` c l r i e k i b i x i l e

l b w` r s `h ie k i~ ~ l ga d ~ a f ~ y ra d ih a f g` f f w & 19"7u-8=} k i g k

i w a x x r i b p x ` h x l x k ` a g g p d p f a x i l e l c i e x r a g ` f f p f a r

g k f l r i h ` i l e w % u9-88 I e x k ` H G E & x k ` r ` a r ` a ~ ~ a r ` e x f y

e l h ` g r ` a w ` w i e G 1 $ g l e h p g x a e g ` p e h ` r f y i e m I ^ W ^ w

a w d i m k x r ` w p f x c r l d h ` w ` e w i x i z a x i l e l c M A B A A

r ` g ` ~ x l r w & :-3 l r e ` m a x i s ` c ` ` h b a g o l c M A B A l e x l

~ r ` w y e a ~ x i g M A B A B r ` g ` ~ x l r w % u¾K

L p r r ` w p fx w h ` d l e w x r a x ` x k a x ` s ` e a w i e m f` I ^ W ^

` s l o ` h b y ^ G w x i d p f a x i l e g a e x ` d ~ l r a r i f y a f x ` r xk `c i r ie m ~ a x x ` r e l c H G E g ` ff w & a e h x k a x x r a i e w l c ` s l o ` h

I ^ W ^ w ` i x k ` r w f l } h l } e l r w x l ~ x k ` b a w a f h i w g k a r m ` l c

H G E e ` p r l e w % I e a h h i x il e & w ~ l e x a e ` l p w I ^ W ^ w l g g p r

i e H G E g ` ff w % X k ` y g l p f h b ` h p ` x l x k ` ~ r ` w ` e g ` l c

i e x ` r e ` p r l e w } i x k i e x k ` e p gf ` i & l r x l w ~ l e x a e ` l p w

h i w g k a r m ` w l c ^ G w % = I d d p e l k i w x l g k ` d i g a f w x p h i `w

~ ` r c l r d ` h l e w ` d i $ x k i e a e h w ` r i a f S i b r a x l d ` w ` g x i l e w

} i x k a e x i b l h i ` w r a i w ` h ie r a b b i x & a m a i e w x M A B A

r ` ~ l r x ` h x k ` ~ r ` w` e g ` l c e ` p r l e w g l e x a i e i e m M A B A

i e H G E % 0-3 K l } ` s ` r & k i w x l g k ` d i g a f ` { ~ ` r i d ` e x w

g a r r i ` h l p x l e g ` r ` b ` f f a r g p f x p r ` w } i x k d l e l g f l e a f

a e x i b l h i` w a m a i e w x M A B A r ` s ` a f` h a s ` r y w d a f f

e p d b ` r l c i e x` r e ` p r l e w f a b ` f` h } i x k ie H G E + F %

R i ` x w g k i e & p e ~ p b f i w k ` h l b w ` r s a x i l e w ) % X k p w & } `

a w w p d ` x k a x x k ` w ~ l e x a e ` l p w I ^ W ^ w h ` w g r i b ` h i e x k `

~ r ` w ` e x w x p h y l r i m i e a x ` h i e w ~ l e x a e ` l p w f y a g x i s ` ^ G w %

GLEGFPWILE

I e w p d d a r y & x k ` ~ r ` w ` e x r ` w p f x w h ` d l e w x r a x ` x k a x

w ~ l e x a e ` l p w I ^ W ^ w a e h I ^ W ^ w ` s l o ` h b y w x i d p f ax i l e

l c ^ G w i e g ` r ` b ` f fa r w f ig ` g p f x p r ` w g a e d l h p f a x ` x k `

c i r i e m l c H G E g ` ff w % ] ` k a s ` ` w x a b f i w k ` h x k a x x k ` w `

I ^ W ^ w a r ` ` e x ir ` fy d ` h i a x ` h b y a g x i s a x il e l c M A B A A

r ` g ` ~ x l r w a e h w k l } f i x x f ` a g x i s i x y $ h ` ~ ` e h ` e x f a b i f ix y %

M i s ` e x k ` k i m k r a x ` l c ^ G h i w g k a r m ` i e w i x p & l p r

l b w ` r s a x i l e w w p m m ` w x x k a x x k ` g ` r ` b ` f f a r g l r x ` { g a e

d a i e x a i e a ~ l } ` r c p f x l e i g i e k i b i x l r y g l e x r l f l s ` r

H G E e ` p r l e w %

A g o e l } f ` h m ` d ` e x w $ $ X k ` ap xk l rw }l p fh f io ` xl xk ae o F %Ri`xwgkie aeh F% K``b clr xk` ~r`~arax ile lc xk` w f ig`gpfxpr`w aw }`ff aw R% W gkl`b clr x`gkeigaf aww iw xaeg`% ]`afwl xkaeo Hr D% Dcikf` xkaf`r clr k iw w~`gic ig gldd`exw lexk i w }l ro & Hrw W % D% Xk l d~ wl e ae h P % M` rb ` r cl r g r i x i g a fr`ahiem aeh glrr`g xile lc xk` daepwgri~ x % Xkiw w xphy }awwp ~ ~ l r x`h b y xk ` Kp dae Crl e x i` r Wg i`e g` ^rl m rad +Mrae xRM 3=,92 B) aeh xk` W}iww Eaxileaf Wgi`eg` Clpehaxile+Mraex 71$2=381%;9)%

R@C@R@EG@W

1% Afbie R% F% & ^rig` R% K% & Waoprai W% Y% & ^`ee`y J % B% aeh Ylpem A% B% +1991) @{gixa xlry aeh iekib ixlry adiel agih

biehiem w i x`w ie kpdae h`exa x` epgf`pw% Braie R`w% 03>& 70>$707%2 % A ema px ^% aeh Wlx` fl G% +19=7) Xk` c ie` w xrpgxpr` lc xk` g`r`b`ffar g`exra f epgf` i ie xk` gax$ $II % Wyea~ xig lrmaeizaxile %

@{~ f Bra i e R`w % 13& 871$808%7 % A phiea x @% & Oe3 ~c`f X% aeh Mcik}if`r B% K% +199>) R`w~lew`w xl `{gixa xlry adiel agihw lc ^proie j` g`f fw aeh e`prle`w

lc xk` h``~ epgf` i ie g`r`b`ffar w f ig` gpfxpr`w % J% ^kywil f % & Fleh% 87>& 29=$717%8 % Ap hieax @% & M,ik}if`r B% K% aeh O ec~ c`f X % +1992) @{gixa xlry wyea~xig ~lx`exia fw ie e`prlew lc xk` h``~ epgf`i ie

lfisl$g`r`b `ffar w f ig` gpfxpr`w % E`prlwgi`eg` 89& 9>7$911%0 % B axie i G% & Bpiww`r` x$H`fdaw G% & Gld ~lie x G% aeh H aei`f K% +19;9) Xk` M AB A`rm ig e`prle`w lc xk` g`r`b`ffar epgf` i

ie xk` ra x : ~rl j`gxilew xl xk` g`r`b`ffar glrx`{ % E`prlwgi % F `x x % 99 & 201$203%3 % Baxie i G% & Gl d~ liex G% & Bpiww`r` x$H`fd aw G% & Haei`f K% aeh Mp`ma e D % +1992) G`r`b`ffar epgf` i aeh xk` epgf`lglrx iga f

~ rl j`g x il e w ie xk ` ra x : r ` x rl m rah ` x r ag ie m gl p ~ f`h xl MABA ae h m fp xada x` iddp e l gk `diw x ry % J % gld~% E`prlf % 710&=8$;8%

= % Gka em ]%& Wxra kf`e hlrc J % G% aeh Wxrak f`ehl rc K% O% +1997) Ileig glexr ibpxilew xl xk` lwgif fa xlry c ir iem agxis i xy lcra x ^proie j` g`f fw ie s i x rl % Braie R`w% 318& 770$781%

; % Gk ae$^ afay S% +19==) Cie` w xrpgxpr` lc xk` farm` e`prlew aeh xk `ir ~rlg`ww`w: h`ehri x ig xklrew ae h xk`ir wyea~w`w% IeG`r`b ` f fa r H `e x a x ` Ep g f `p w Gka~% 8 & ~~ % ;3$128% W~riem`r & E`} Ylro%

9 % G lprsif f` J % & Apmpwxie` J % R% aeh Da rx` f ^% +19==) ^rlj`gxilew crld xk` iec` r ilr l f is` xl xk` g`r`b`ffar epg f i ie xk`gax h`dle wxra x`h by r` xrlmrah` xraew~l rx l c klrw`rahiwk ~`rl{yhaw` % Braie R`w% 17>& 8>0$819%

I> % H asi`w G% K& & Hasi` w W% E% aeh Glff iem rihm` M% F% +199>) ^air`h$~pfw` h`~r`wwile lc dlelwyea~ xig MA BA $d` hiax`hiekibixlry ~lwxwyea~xig r`w~lew`w ie ra x k i~~lgad~pw% J% ^kywil f % & Fleh% 828& 017$071%

1 % H`iwz R% A% aeh ^rieg` H% A% +19;9) H`~`eh`ex h`~r`wwile lc iekib ix ile ie mpie`a$~im e`lglrx`{ ie sixrl b y M A B A ar`g`~ xl r c ``h $b ago l e MABA r` f`a w` % J% ^kywil f % & Fleh% 812& 017 082%

12% @ggf`w J % G% +19=7) Xk` g`r`b`ffpd aw a gld~px`r: ~ax x`rew ie w~ag` aeh x id`% J% ^kywil f % & Fleh% 229& 1$72%17% C lee pd C% & Wxlrd$D axkiw`e J % aeh ] afb`rm C% +19=>) Mfp xad ax` h`garbl{yfaw` ie iekib ixlry e`prlew% A w xphy lc xk`

`ezyd` ie ^proie j` g`f fw a{le aeh blpxlew ie xk` gax % Braie R`w% 2>& 209$2=0%1 8% Cl e e p d C% ae h ]afb ` rm C % +1 9= 7 ) Ae ` w x ida x il e l c = $adie l b p xy r ig ag ih ae h m fp xada x` h `ga rb l { y fa w` ie xk ` ie k ib i xl ry

a{le x`rdieafw ie xk` gax % Bra i e R`w % 08& 110$12=%10% Mcik}if`r B% K% +19;1) Lrmaelxy~ig dlelfay`r gpfxpr`w lc e`rslpw x iwwp` % J% E`prlwgi % D`xk % 8& 729$782%13% Mu&k}if`r B% K% & Xkld~wle W% D% & Aphieax @% aeh Rlb`rxwle R% X% +1991) Lrmaelxy~ig w f ig` gpfxpr`w lc e`praf x iwwp` %

Ie Gpf xpriem E`rs` G`f fw +`hw Baeo`r M% aeh Mlwfie O%) Gka~% 7 & ~~ % 7=9$812% DIX ^r`ww & Gadbrihm`& DA%1 =% Mrl `e `e }`m `e K% J % ae h Sl l m h J % +19 = =) Xk ` ~ a ra wam i x x a f zl e a x il e } i xk ie xk ` l f is l g` r`b ` ffa r ~ rl j`g x il e $ $ f % G f idb ie m

cib`r h iw xr ibpxile ie xk` s`rdiw lc ga x g`r`b`ffpd% J% gld~% E`prlf % 1=8& 81=$8;;%

Page 14: D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the Rat Cerebellum in Vitro

8/3/2019 D. Mouginot and B.H. Gahwiler- Characterization of Synaptic Connections Between Cortex and Deep Nuclei of the …

http://slidepdf.com/reader/full/d-mouginot-and-bh-gahwiler-characterization-of-synaptic-connections-between 14/14

=1 2 H % Dl p m i e l x ae h B % K % M u i k } i f ` r

1 ;% K ad l r i J % a e h D`z`y @ % +1 9 = =) W` r i a f ae h x r i ah i g wy e a~ w`w i e x k ` g` r`b ` f f a r e p g f ` i l c x k ` ga x % @ { ~ f B r a i e R ` w % 7 > &

2 0 9 $2 = 7 %1 9 % K p m p `e a rh J % R % ae h A f m ` r B % @ % +1 9 ; 3 ) ] k l f ` $g` f f s l f x am ` $g f ad~ w x p h y l c x k ` cah i e m l c M A BA $ag x i s a x `h gp r r`e x w i e

agp x ` f y h i w wl g i a x `h k i ~ ~ l gad~ a f e `p rl e w % J % E` prl~ky w i l f % 03& 1$1; %2 > % K `e h ` f d ae ] % J % ae h D arwk a f f O % G % +1 9 ;> ) A { l e a f ~ rl j `g x i l e ~ a x x ` re w s iwp a f i z`h } i x k k l rw` rah i wk ~ ` rl { y h aw` i e

l rm ae i z`h gp f x p r` w l c g ` r`b ` f f p d% E` prlwg i ` eg ` 0& 1;77$1;83%2 1 % Io `h a D % ae h Da x wp wk i x a D% +1 9 = 7) @ f `g x rl e d i g rl wgl ~ i g l b w` rs a x i l e w l e x k ` w~ i e a f ~ rl j `g x i l e w x l x k ` g` r`b ` f f a r e p g f ` i

i e x k ` ga x ae h rab b i x % @ { ~` r i ` e x i a 29 & 12;>$12;2%2 2 % I xl D% a e h Y l wk i h a D% +1 9 3 3) Xk ` l r i m i e l c g` r`b ` f f a r $ i e h p g`h i e k i b i x i l e l c H ` i x ` r w e `p rl e ` w $ $ I % D l e l wy e a~ x i g

i e i x i a x i l e l c x k ` i e k i b i x l ry ~ l w x wy e a~ x i g ~ l x `e x i a f w % @ { ~ f B r a i e R ` w % 2& 77>$789%2 7 % I x l D% & Y l wk i h a D % ae h L b a x a O % +1 9 38 ) D l e l w y e a~ x i g i e k i b i x i l e l c x k ` i e x rag` r`b ` f f a r e p g f ` i i e h p g`h c rl d x k `

g` r`b ` f f a r gl r x `{ % @ { ~` r i ` e x i a 2>& 0=0 0=3%2 8 % I x l D % & Y l wk i h a D % ae h L b a x a O % +1 9 = > ) Ie k i b i x l ry gl e x rl f l c i e x rag` r`b ` f f a r e p g f ` i b y ^p ro i e j ` g`f f a{ l e w % @ { ~ f B r a i e

R ` w % 1>& 38$;> %2 0 % Jak e w`e K % +1 9 ; 3 ) @ f `g x rl ~ k y w i l f l m i ga f gk a rag x ` r i w x i g w l c e `p rl e `w i e x k ` m p i e `a $~ i m h ``~ g` r`b ` f f a r e p g f ` i ie sixrl%

J % ^ ky w i l f % & F leh % 7=2& 129$18=%2 3 % Ja k e w` e K % +1 9; 3 ) @{ x rag` f f p f a r a g x i s a x i l e ae h d `d b rae ` gl e h p g x ae g`w l c e `p rl e `w i e x k ` m p i e `a $~ i m h ``~ g` r`b ` f f a r

e p g f ` i i e s i x rl % J % ^ ky w i l f % & F leh % 7=2& 189$13;%2 = % F f ae l I % & M u i k} i f` r B % K % ae h Da r x y A % +1 99 2 ) S l f x am ` $ ae h x rae wdi x x ` r $m a x `h gk ae e ` f w ie ^p ro i e j ` g` ff w c rl d

l rm ae l x y ~ i g g` r`b ` f f a r gp fx p r` w % Ie X k` G` r` b` f f pd R ` s i w i x ` h +`hw Ff ieuiw R% a eh W lx`fl G%) % Gk a~% 2 & ~~ % 1;2$2>1%

W ~ r i e m ` r & E ` } Y l r o %2 ; % F f i ec iw R % +1 9 ; ; ) Xk ` i e x r i e w i g ` f `g xrl ~ k y w i l f l m i ga f ~ rl ~ ` r x i ` w l c dad da f i ae e `p rl e w : i e w i m k x w i e x l g`e x ra f e ` rs l p w

wy w x `d cp e g x i l e % Wg i ` eg ` 282& 1308 1338%2 9 % F f ie c iw R % ae h J ak e w `e K % +1 9 ;2 ) @ f `g x rl ~ k y w i l f l m y l c dad daf i a e x k a f adi g e `p rl e `w ie s i x rl% Eaxpr` 2 9 = & 8 > 3 $8 > ; %

7 > % F f ie c iw R % a e h Dci k f ` x k a f ` r D % +1 9; ; ) @ f `g x rl ~ k y w i l f l m y l c m p i e `a $~ i m g` r`b ` f f a r e p g f `a r g` ff w i e x k ` ie s i x rl b r a i e

w x `d$g` r`b ` f f a r ~ r`~ a ra x i l e % J % ^ ky w i l f % & F leh % 8 > 8 & 2 8 1 $2 0 ; %7 1 % D gGa rr`e D % ae h A f m ` r B % @ % +1 9; 0 ) P w` $h `~ `e h `e x h `~ r` ww i l e l c I^W^w i e ra x k i ~ ~ l gad ~ a f ~ y radi h a f g ` ff w ie s i x rl%

J % E` prl~ky w i l f % 0 7 & 0 0 = $0 = 1 %7 2% D g G l r d i g o H % A % + 1 99 >) D ` d b r a e ` ~ r l ~ ` r x i ` w a e h e ` p r l x r a e w d i x x ` r a g x il e w % I e X k` Wy ea~ x i g L rmae i za x i le l c x k` B ra i e

+ `h % W k ` ~ k ` r h M % D % ) % G k a ~ x ` r 2 & ~ ~ % 7 2 u 3 % L { c l r h P e i s ` r w ix y ^ r ` ww & E ` } Y l r o %7 7 % Da rwk a f f O % G % & ] l j x l } i gz J% D% ae h K `e h ` f d ae ] % J % +1 9; > ) ^a x x ` re w l c cp e g x i l e a f wy e a~ x i g gl e e ` g x i l e w ie l rm ae i z`h

gp f x p r` w l c g ` r`b ` f f p d% E` prlwg i ` e` ` 0& 1;8= 1;0=%7 8 % D l r i wk i x a ] % ae h Waw x ry B % R % +19 9 7) F l e m $ x ` rd h `~ r` ww i l e l c I^W^w i e ra x h ``~ g` r`b ` f f a r e p g f ` i% E ` p r l R ` ~ l r x 8 &

= 1 9 $= 2 2 %7 0 % Dp r i R % a e h O e 3 ~ c` f X % +1 99 8 ) A g x i s i x y i e h p g`h ` f `s a x i l e w l c i e x rag` f f p f a r g a f g i p d gl e g`e x ra x i l e i e e `p rl e w l c x k `

h ``~ g` r`b ` f f a r e p g f ` i % J % E` prl~ky w i l f % = 1 & 8 2 > $8 2 9 %7 3 % E p d ae e R % @ % ae h ] l e m R % O % W % +1 9 ;8 ) S l f x am ` $g f ad~ w x p h y l e M A BA r` w~ l e w` h ` w`e w i x iz a x i l e i e w i e m f ` ~ y rad i h a f

g` f f w h i w wl g i a x `h c rl d x k ` k i ~ ~ l gad~ p w l c ah p f x m p i e `a $~ i m % E` prlwg i % F ` x x % 8=& 2;9 298%7 = % L b a x a O % +1 9 39 ) M A BA i e ^p ro i e j ` g` ff w ae h d l x l e `p r l e `w % @ { ~` r i ` e x i a 20& 12;0%7 ; % L b a x a O % & I x l D% & L gk i R % ae h Wa x l E % +1 9 3 = ) ^k a rd agl f l m i ga f ~ rl ~ ` r x i ` w l c xk ` ~ l w x wy e a~ x i g i e k i b i x i l e b y ^p ro i e j `

g` ff w a{ l e w & ae h x k ` ag x i l e l c m add a$ad i e l b p x y r i g ag ih l e H ` i x ` r w e `p rl e `w % @ { ~ f B r a i e R ` w % 8 & 8 7 $0 = %

7 9 % L b a x a O % & Xa o `h a O % ae h Wk i e l w ao i K % +1 9 => ) Cp r x k ` r w x p h y l e ~ k a rdag l f l m i ga f ~ rl ~ ` r x i ` w l c x k ` g` r`b ` f f a r $ i e h p g`h

i e k i b i x i l e l c H ` i x ` r w e `p rl e `w % @ { ~ f B r a i e R ` w % f f & 72= 782%8 > % L f ~ ` K % & O a r f w wl e M % & ^l zza D % C % & B rp m m ` r C % & W x ` i e d ae e D% & S ae R i `z`e K % & Cam m M % & K a f f R % M % & Crl `w x f ] % ae h

Bi x x i m ` r K % +1 9 9 > ) GM ^ 7 0 78 ; : a g`e x ra f f y ag x is ` b f l go ` r l c M A B A B r`g`~ x l rw % @ pr % J % ^ kard ag % 1;=& 2= 7;%8 1 % ^a f o l s i x w D % & D `z`y @ % & K c i dl r i J % ae h W z`e x c i m l xk a i J % +1 9= = ) Q p ae x i x a x i s ` k i w x l f l m i ga f ae a f y w i w l c x k ` g` r`b ` f f a r e p g f` i

i e x k ` ga x $ $ I % E p d`r i ga f h a x a l e g` f f w ae h wy e a~ w`w % @ { ~ f B r a i e R ` w % 2 ; & 1;9$2>9%8 2 % ^ i e ap f x H % ae h H `wgk 3 e `w D% +1 9 9 2) Xk ` l r i m i e l c rk y x k d i g ca w x wp b x k r` wk l f h h `~ l f a r i za x i l e w i e x k a f adi g r` f ay g` ff w

l c r a x w p e h ` r p r ` x k a e ` a e a ` w x k ` w i a % B ra i e R ` w % 090& 290$7>>%8 7 % Wa i x l O % & Ba rb ` r R % & ] p J % Y %& Da x w p h a X % & R l b ` r x w @ % ae h S ap m k e J % @% +1 9 =8 ) Idd p e l k i w x l gk `d i ga f f l ga f i za x il e

l c m f p x ada x ` h `ga rb l { y f a w` i e ra x g ` r`b ` f f p d% ^ rlg % ea x e % A g ah % W` i % P % W % A % =1& 239 2=7%8 8 % W`m a f D% a e h Ba ro ` r J% F % +1 9; 8 ) Ra x k i ~ ~ l gad ~ a f e `p rl e `w i e gp f x p r` : ~ rl ~ ` r x i ` w l c M A BA $ag x i s a x ` h G1 i l e

g l e h p g x a e g ` % J % E` prl~ky w i l f % 01& 0>> 0>0%8 0 % W x ` r i ah ` D% & G p r rl $H l ww i R % & ^a r3 H % ae h L ao wl e M % +1 99 1 ) Caw x l wg i f f a xi l e w +2 > $8 > K z) i e xk a f ad l gl r x i g a f wy w x `dw

ae h x k ` i r ~ l x `e x i a x i l e b y d`wl ~ l e x i e ` gk l f i e ` rm i g e p g f ` i i e x k ` ga x % ^ rlg % ea x e % A g ah % Wg i % P % W % A % ; ; & 8 7 9 3 $8 8 > > %

8 3 % Xk l d ~ wl e W % D% & H ` i wz R % A % ae h ^r i e g` H % A % +1 9; ; ) R` f a x i s ` gl e x r i b p x i l e w l c ~ a ww i s ` q p i f i b r i p d ae h ag x i s ` x rae w~ l r x

x l x k ` h i w x r i b p x i l e l c g k f l r ih ` i e d a d d a f i a e g l r x i g af e ` p r l e w % J % E` prl~ky w i l f % 3> & 1>0 128%8 = % X k l d ~ wl e W % D% ae h M c i k } i f ` r B % K % +1 9 ;9 ) A g x i s i x y $h `~ `e h ` e x h i w i e k i b i x i l e $ $ f % R`~ ` x i x i s ` w x i dp f a x i l e r`h p g`w I^W^

h r i s i e m c l r g ` a e h g l e h p g x a e g ` i e x k ` k i ~ ~ l g a d ~ p w ie s i x rl% J % E`prl~kywilf % 3 1 &0 > 1 0 1 1 %8 ; % X k l d ~ wl e W % D% ae h M u k } i f ` r B % K % +1 9 92 ) @c c`g xw l c xk ` M A BA p ~ x ao ` i e k i b i x l r x i am ab i e ` l e ie k i b i x l ry wy e a~ x i g

~ l x `e x i a f w i e ra x k i ~ ~ l gad~ a f w f i g ` gp f x p r` w % J % E` prl~ky w i l f % 3=& 139; 1=>1%8 9 % X p rm `l e W % D% ae h A f b i e R % F % +1 99 7 ) ^k a rd agl f l m y & h i w x r i b p x i l e & g ` ff p f a r f l ga f i za x i l e ae h h `s ` f l ~ d `e x l c M A B A B

b i e h i e m i e rl h `e x g` r`b ` f f p d% E` prlwg i ` eg ` 00 & 711 727%0 > % S ae H `r ] a e m J % J % F %& ] i o f p e h F % & M p `m ae D% & R p i m rl O % X % ae h S l l m h W % +1 9; 9 ) A e x ` rl m rah ` x rag i e m l c x k ` ra x

l f i s l g` r`b ` f f a r wy w x `d } i x k ^ kaw` l f pw s p f mar i w f `p gl am m f p x i e i e +^K A $F) % H `d l e w x ra x i l e l c g f i db i e m c i b ` r gl f f a x ` ra fi e e ` rs a x i l e l c x k ` g` r`b ` f f a r e p g f ` i % J % g ld ~ % E` prl f % 2;;& 1$1;%

0 1 % ] aw w` c D% & W i d l e w J % & Xa~ ~ az D% ae h Wl x ` f l G % +1 9; 3 ) E l e $^p ro i e j ` g` ff w M A BA `rm i g i e e ` rs a x i l e l c x k ` h ``~

g` r`b ` f f a r e p g f ` i : a q p ae x i x a x i s ` i ddp e l gy x l gk `di ga f w x p h y i e G0 = BF ae h i e ^p ro i e j ` g` f f w h `m `e ` ra x i l e dp x ae x di g` %

B ra i e R ` w % 799& 120 170%0 2 % ] l j x l } i gz J % D% & D arwk a f f O % G % ae h K `e h ` f d ae e ] % J % +1 9 =; ) @ f `g xrl ~ k y w i l f l m i ga f ae h ~ k a rd agl f l m i ga f w x p h i ` w l c

x k ` i e k i b i x l ry ~ rl j `g x i l e c rl d x k ` g` r`b ` f f a r gl r x `{ x l x k ` h ``~ g` r`b ` f f a r e p g f ` i i e x i w wp ` gp f x p r` % E` prlwg i ` eg ` 7 &

3 > = u 1 ;%

+ A g g ` ~ x ` h 1 W ` ~ x ` d b ` r 1998)