Cytoskeletal Regulation of Membrane Function

Cytoskeletal Regulationof Membrane Function

Society of General Physiologists • 50th Annual Symposiu m

Edited by

Stanley C . Froehner

University of North Carolina at Chapel Hill ,

Chapel Hill, North Carolin a

and

Vann Bennett

Duke University, Durham, North Carolina

Preface

ix

Chapter 1

Actin-Membrane Interactions

Actin-binding Membrane Proteins Identified b yF-actin Blot OverlaysElizabeth J. Luna, Kersi N. Pestonjamasp,Richard E. Cheney, Christopher P. Strassel,Tze Hong Lu, Catherine P. Chia, Anne L. Hitt,Marcus Fechheimer, Heinz Furthmayr,and Mark S. Mooseker

3

Interactions between Dystrophin and th eSarcolemma Membran eJeffrey S. Chamberlain, Kathleen Corrado, Jill A . Rafael,Gregory A . Cox, Michael Hauser, and Carey Lumeng

19

A Multiple Site, Side Binding Model for the Interactio nof Dystrophin with F-ActinJames M. Ervasti, Inna N. Rybakova, and Kurt J. Amann

31

Chapter 2

Establishment of Epithelial Cell Polarity

Roles of the Membrane Cytoskeleton in Protein Sortin gW. James Nelson, Kenneth A . Beck,and Peter A . Piepenhagen

4 7

Role of Cytoplasmic Motors in Post-Golgi Vesicular Traffi cAnne Müsch and Enrique Rodriguez-Boulan

55

Molecular Architecture of Tight Junctions :Occludin and ZO- 1Shoichiro Tsukita, Mikio Furuse, and Masahiko Itoh

69

Chapter 3

Spectrin and Associated Protein s

Capping Actin Filament Growth: Tropomodulin inMuscle and Nonmuscle Cell sVelia M. Fowler

79

Functional Studies of the Membrane Skeleton i nDrosophila : Identification of a Positional Cue thatTargets Polarized Membrane Skeleton Assembl yRonald R. Dubreuil, Gary R . MacVicar, an dPratumtip Boontrakulpoontawee Maddux

91

Molecular Architecture of the Specialized AxonalMembrane at the Node of RanvierVann Bennett, Stephen Lambert, Jonathan Q. Davis,and Xu Zhang

107

Chapter 4

Specialized Membrane Domains an dTheir Cytoskeletal Connection s

Specificity of Desmosomal Plaque Protein Interaction swith Intermediate Filaments: Keeping Adhesiv eJunctions SegregatedKathleen J. Green, Elayne A. Bornslaeger,Andrew P. Kowalczyk, Helena L. Palka,and Suzanne M. Norvell

123

Intermediate Filament Linker ProteinsElaine Fuchs, Yanmin Yang, James Dowling ,Panos Kouklis, Elizabeth Smith, Lifei Guo,and Qian-Chun Yu

141

Functional Studies of the Protein 4 .1 Family ofJunctional Proteins in DrosophilaRichard G. Fehon, Dennis LaJeunesse, Rebecca Lamb ,Brooke M. McCartney, Liang Schweizer ,and Robert E. Ward

149

Chapter 5

Cytoskeletal Interaction with Ion Channels

Cytoskeletal Interactions with Glutamate Receptor sat Central SynapsesGary L. Westbrook, Johannes J. Krupp, and Bryce Vissel

163

A Membrane Skeleton that Clusters Nicotini cAcetylcholine Receptors in MuscleRobert J. Bloch, Gabriela Bezakova, Jeanine A. Ursitti,Daixing Zhou, and David W. Pumplin

177

Syntrophins : Modular Adaptor Proteins at theNeuromuscular Junction and the SarcolemmaStanley C. Froehner, Marvin E. Adams, Matthew P. Peters,and Stephen H. Gee

197

Mechanical Transduction by Ion Channels : How Force sReach the Channe lFrederick Sachs

209

Chapter 6

Signaling via the Membrane Cytoskeleton

Cell Signalling and CAM-mediated Neurite OutgrowthFrank S. Walsh, Karina Meiri, and Patrick Doherty

221

Dissection of Protein Kinase and Phosphatas eTargeting InteractionsJohn D. Scott

227

Signaling through the Focal Adhesion Kinas eMichael D. Schaller

241

Involvement of the Actin Network in Insulin Signallin gTheodoros Tsakiridis, Qinghua Wang, Celia Taha ,Sergio Grinstein, Gregory Downey, and Amira Klip

257

List of Contributors

273

Subject Index

277