Cytomorphology of Normal Blood Cell Zhaoxindong E-mail:[email protected].

Cytomorphology of Normal Blood Cell

Zhaoxindong

E-mail:[email protected]

erythron

pronormoblast

Early normoblast:basophilic

Intermediate normoblast: polychromatic

Late normoblast:

pronormoblast Shape:round or oval

15-20um in diameter

Nucleus:

large,round or oval

reddish purple in color

granulous chromatin

Pattern with 1-2 nucleoli

Cytoplasm:

deep blue and opaque

Small amount,may show

Smalltumorlike protrusion

Early normoblast:basophilic

Shape:round or oval

10-18um in diameter

Nucleus:

large, round or oval

chromatin pattern slightly coarser ,

nucleoli indistinct

Cytoplasm:

deep blue and opaque

Small ,perinuclear pale zone is obvious

Intermediate normoblast: polychromaticShape:round or oval

8-15um in diameter

Nucleus:

smaller,round or oval

chromatin more condensed

no nucleoli

Cytoplasm:

blue-gray to pink-gray

Late normoblast:

Shape: round

7-10um in diameter

Nucleus:

pyknotic nucleus

Cytoplasm:

Pinker than previous stage

Mitoic figure

Prophase: 线团期 Metaphase: 菊花期，染色体分析的最佳时期 Anaphase: 双极期 Telophase: 双子星期

Myelocyte series

Myeloblast

Promyelocyte

Myelocyte

Metamyelocyte

Band neutrophil

Segmented neutrophil

Eosinophlic granulesthe neutrophlic granules are fine and stain a

blue-pink color with Wright stain. the neutrophlic granules are fine and stain a

blue-pink color with Wright stain. are larger than Neutrophilic granules. These round and oval-shaped granules are orange and have a

Glassy or semiopaque texture. basophilic granules have a dark blue-back color and dense appearance.

Myeloblast

This cell has an average overall diameter of 10 to 20um,the nuclear chromatin is finely reticular,with 2 to 5 light-staining nucleoli. the cytoplasm is sky blue , small in amount and lacks granules.

Promyelocyte

The cell is larger than the blast stage, with an average diameter of 12 to

The nuclear chromatin is more condensed than in the blast, and nucleoli are present. the cytoplasm is a pale grayish blue. Cytoplasm have a few azurophilic granules .

Myelocyte The cell has an

average diameter of 10 to 20um. The nucleus has a more oval appearance than in previous stages, no nucleoli, and the chromatin is much more clumped than in previous stages. Cytoplasm have a few neutrophilic granules .

METAMYLOCYTE

The cell most characteristic feature is that the nucleus begins to assume an indented or kidney-bean shape. Which will continue to elongate as the cell matures through this phase.

The chromatin continues to become more condensed or clumped.

Band granulocytes

Band granulocytes has a typical elongated nucleus.

The chromatin is very clumped.

The cytoplasm have a fine and abundant specific pink neutrophilic granules .

The segmented granulocytes

The cell has a characteristic multilobed nucleus, the separate lobes are attached to each other by a fine thread like filament.

megakaryocytic series

Megakaryoblast Promegakaryocyte Granular megakaryocyte Platelet-producing megakaryocyte Nude nucleus megakaryocyte Platelet

Megakaryoblast

The cell size can be vary from 15 um to over 50 um in diameter. it is a round cell The compact nucleus has an overall color of pink and the chromatin is a red-purple color, normally, megakaryoblasts have several nucleoli, the nucleus is surrounded by basophilic cytoplasm. cytoplasmic pseudopods are frequently seen. No granules.

Promegakaryocyte

The cell size can be vary from 30 um to over 50 um in diameter ,

the chromatin continues to darken in color with increased clumping seen in the nuclear pattern, two or more distinct nucleoli may be seen. The quantity of cytoplasm increases; the basophilic color persist .pseudopods are frequently seen. Fine azurophilic granules are most abundant adjacent to the nucleus.

Granular megakaryocyte

Megakaryocytes are the largest bone marrow cells with an average size of up to 100 um,

no nucleoli distinctive feature of the megakaryocyte is that it is multilobular, not multinucleated,

Platelet-producing megakaryocyte

on completion of maturation the fully mature lobes of the magakaryocyte shed platelets excplosively from the edges of the cytoplasm.

Nude nucleus megakaryocyte

Platelets

Platelets have an average diameter of 2 to 4 um with younger platelets being large than older ones. platelets have no nucleus, the cytoplasm is light blue with evenly disperped, fine red-purple granules.

Plasmacyte series

Plasmablast

Proplasmacyte

Plasmacyte

Plasmablast

Shape:round and elliptical 14-18um in diameterNucleus: round or oval and

eccentric. reticular chromatin , nucleoli indistinctCytoplasm: basphilic and opaque

cytoplasm.abundant and vacuolate without granules

ProplasmacyteS:round and elliptical

12-16um in diameter

N:round or oval and eccentric.

chromatin coarser and more clumped than in the plasmablast stage ,

nucleoli invisible.

C: basphilic, paranuclear clear zone may become visible, vacuolate, nongranular or a few

Plasmacyte

S:round and elliptical

12-15um in diameter

N: round or oval and eccentric with nucleoli wheel spoke chromatin pattern

C:deeply basphilic, with vacuoles or frothy,nongranular