Curriculum Vitae Niraj H. Tolia, Ph.D. updated 12/22/2006 1 of 4 PERSONAL DETAILS Name: Niraj H. Tolia, Ph.D. Present Position: Postdoctoral Fellow Keck Structural Biology Laboratory. Address: Cold Spring Harbor Laboratory 1 Bungtown Road Cold Spring Harbor NY 11724. Tel: (631) 988-0627, (516) 367-8867 Fax: (516) 367-8873 e-mail: [email protected]RESEARCH EXPERIENCE Apr 2004 – present Postdoctoral research, Cold Spring Harbor Laboratory (CSHL), Cold Spring Harbor, NY, USA. • Structural, biochemical and mechanistic studies of complexes involved in RNA interference. • Developed a recombinant co-expression system for production of Argonaute (Ago) proteins. • Performed kinetic and mechanistic characterization of cleavage by recombinant human Ago2 with implications for RISC slicer activity. • Demonstrated S. pombe Ago1 is a bona fide slicer and requires slicer activity for heterochromatic silencing. • Identified a novel clade of worm specific Argonautes by a phylogenic analysis. • Designed and developed a crystallization system allowing for predictive feed forward screening. Successfully predicted and obtained crystallization conditions for three different protein complexes. • Preliminary crystallographic analysis of the binding domain of a malarial surface antigen in complex with a F ab fragment of a neutralizing antibody Sep 1999 – Mar 2004 Ph.D. thesis research, Watson School of Biological Sciences (WSBS), CSHL, NY, USA. Research Advisor: Professor Leemor Joshua-Tor • Crystallographic structure determination of the binding domain of a malarial surface antigen involved in erythrocyte invasion, unbound and in complex with a small molecule containing the essential components of the receptor for the antigen. • Confirmed model of erythrocyte binding using a mutational analysis coupled to a semi- quantitative mammalian cell culture binding assay. May 1999 – Sep 1999 Undergraduate thesis research, Imperial College of Science Technology and Medicine, London. UK. Research Advisor: Dr. Steven Matthews • Expressed and purified an archael RNA polymerase domain for NMR studies. Sep 1997 – Oct 1998 Industrial Trainee, Drug Metabolism Department, Pfizer Central Research, Sandwich, Kent. UK. Research Advisor: Dr. Mark J. Ackland • Developed an automated system for in vitro drug metabolism assays. • Applied this system to active site characterization of cytochrome P450 2D6, using SAR studies and modeling.
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Curriculum Vitae Niraj H. Tolia, Ph.D.tmp.wustl.edu/archives/TMPSpring2007/Tolia_CV.pdfCurriculum Vitae Niraj H. Tolia, Ph.D. updated 12/22/2006 3 of 4 PUBLICATIONS (CONTINUED) •
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