CURRICULUM VITAE - McGill Universitypublications.mcgill.ca/.../04/CV-Dr.-Michael-Ratcliffe.pdf1 CURRICULUM VITAE Michael John Hayward Ratcliffe Department of Immunology, University

1

CURRICULUM VITAE

Michael John Hayward Ratcliffe

Department of Immunology,University of Toronto,Medical Sciences Building,1 King’s College Circle,Toronto, Ontario,Canada M5S 1A8

Sunnybrook Health Sciences Center,2075 Bayview Avenue, Room A3-31,Toronto, Ontario,Canada, M4N 3M5

(416)-978 6382(416)-480 6103(416)-978 1938

[email protected]

August 8, 1954Dual national: British/CanadianMarried, two children (born 1988 and 1990)

January 2010

Ph.D. 1980. University College London, England. Immunology.B.Sc. 1976. The University of Glasgow, Glasgow, Scotland. Honours Biochemistry.

2001-present Professor and Chair, Department of Immunology, University of Toronto,Toronto, Ontario, Canada.

DR. MICHAEL J.H. RATCLIFFE

Name:

Office address:

Lab address

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e.mail:

Date of Birth:Nationality:Marital status:

A. Date of CV preparation

B. Biographical information

1. Degrees:

2. Employment:

2

2001-present Senior Scientist, Discipline of Molecular and Cell Biology, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada.

2001-present Full Member, School of Graduate Studies, University of Toronto.

2009-present Member of Senate, Trinity College, University of Toronto.

2007-present Fellow, Trinity College, University of Toronto.

2003-2007 Associate Fellow, Trinity College, University of Toronto.

2005-2006 Interim Discipline Director, Molecular and Cell Biology, SunnybrookHealth Sciences Center, Toronto, Ontario, Canada.

1998-2001 Professor, Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

1991-1998 Associate Professor, Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

1991 Tenure awarded, McGill University.

1986-1991 Assistant Professor, Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

1983-1986 I.C.R.F. Research Fellow, Imperial Cancer Research Fund, Tumour Immunology Unit, University College London, London, U.K.

1980-1983 Member, Basel Institute for Immunology, Basel, Switzerland.

1993-1996 Fonds de la Recherche en Santé du Québec Chercheur-boursier (Senior 2).

1990-1993 Fonds de la Recherche en Santé du Québec Chercheur-boursier (Senior 1).

1987-1990 Fonds de la Recherche en Santé du Québec Chercheur-boursier (Junior 2).

1983-1986 Imperial Cancer Research Fund Research Fellowship.

1976-1980 Wellcome studentship.

British Society for Immunology: 1978-present Member.

3. Honours

4. Professional affiliations and activities:

4.1 Societies

3

Canadian Society for Immunology: 1988-present Member, 1991-1993 Member of council.1993-1995 Re-elected to council.1999-2001 Vice-President.2001-2003 President.2003-2005 Past-President.2008-present Outreach councilor.

Canadian Federation of Biological Societies:1999-2003 Member of Executive Council.2004-2005 President-Elect.2005-2007 President.2007-2009 Past President.

American Association of Immunologists: 2001-present Member.

International Union of Immunology Societies: 1991-2001 Chair: Avian CD Nomenclature Committee.2001-2010 Member: General Assembly (one of two Canadian members).

Panel membership:1989-1991 Member: FRSQ panel for graduate studentships (M.Sc. level).1991-1993 Member: Cancer Research Society Fellowships Committee.1993-1996 Member: MRC Immunology and Transplantation Operating grants

panel.1996-1999 Scientific Officer: MRC Immunology and Transplantation

Operating grants panel.2000-2003 Chairman: CIHR (formerly MRC) Immunology and Transplantation

Operating Grants Panel.2003-2004 Invited member: CIHR Multi-User Equipment and Maintenance

grant panel.2004-2005 Member: CIHR Multi-User Equipment and Maintenance grant

panel.2004 Member: Ontario College of Graduate Studies; site visit reviewer

for Department of Microbiology and Immunology, University of Western Ontario.

2004-present Member: College of Reviewers, Canada Research Chairs.

4.2 Grant review:

4

2005-2006 Invited Chair: CIHR Immunology and Transplantation Operating grants panel.

2006-2009 Chairman: CIHR Immunology and Transplantation Operating grants panel B.

2006-2008 Member: Ontario Research Fund: Research Excellence Committee.2007-2009 Member: Canada Research Chairs Interdisciplinary Adjudicating

Committee.2007 Member: International Review Committee for the Institut de

recherches cliniques de Montréal.2009 Member: CIHR Strategic Training Program Grant panel B.

Ad hoc reviewer:Fonds de la Recherche en Santé du QuébecMedical Research Council of CanadaNatural Sciences and Engineering Research Council of CanadaNational Cancer Institute of CanadaCanadian Institutes of Health ResearchSaskatchewan Health Research FoundationBritish Columbia Health, Michael Smith Health ResearchAlberta HeritageUnites States Department of AgricultureNational Institutes of Health, USANational Science Foundation, USAScience Foundation Ireland

Guest editor: Seminars in Immunology, volume 2 issue 3, 1990. Saunders Scientific Publications.

International Editorial Advisory Board: Veterinary Immunology and Immuno-pathology, 1999-present.

Editorial Advisory Board: Seminars in Immunology 2001-present.Editor-in-Chief: Encyclopedia of Immunology 3rd Edition. Elsevier;. 5 volumes,

3750 pages. Publication targeted 2011.

Ad hoc reviewer:Canadian Journal of Allergy and Clinical Immunology, Cellular Immunology, Development and Comparative Immunology, Developmental Dynamics, European Journal of Immunology, Experimental Molecular Pathology, Immunobiology, Immunology, Immunological Investigations, Immunology Letters, International Immunology, Journal of Anatomy, Journal of Clinical Immunology, Journal of Experimental Medicine, Journal of Immunology, Journal of Leukocyte

4.3 Editorial boards

B Lymphocyte Development.

5

Biology, Journal of Molecular Medicine, Nature, Oncogene, Trends in Immunology, Veterinary Research, Veterinary Immunology and Immunopathology.

Moore and Van Allen Pllc., Attorneys at Law (Durham, North Carolina); Expert witness reports and testimony in patent litigation, 1997-1998.

Member: Scientific Advisory Board: Origen Therapeutics (Burlinghame, CA, USA), 2000-present.

4.5.1 Departmental (1986-2001, McGill University; 2001-present, University of Toronto)

Immunology Promotions and Appointments Committee (2001-present)Immunology Undergraduate Committee (2001-present)Immunology Graduate Committee (2001-present)Immunology Faculty Search Committees (2001-present)Microbiology and Immunology Animal Care Committee (member 1986-

1994, Chair 1994-2001).Microbiology and Immunology Undergraduate Committee (member

1989-2001).Microbiology and Immunology Graduate Committee (member 1993-

2001)Microbiology and Immunology Curriculum Review Committee (member

1995-1996).Microbiology and Immunology Seminar Committee (member 1987-1989,

chair 1989-1994, member 1998-1999).

4.5.2 Faculty and University(1989-2001, McGill University; 2001-present, University of Toronto)

University of Toronto Transplantation Institute Executive Committee (member, 2009-present).

University of Toronto Bacardi Chair in Transplantation search committee (member, 2009-present).

Dean of Medicine’s Advisory Committee on International Research (2009-present).

4.4 Consultancies

4.5 University committees

6

Ontario Cancer Institute Search Committee for Clinician Scientist in Immunotherapy (member, 2009-present)

Dean of Medicine’s Advisory Committee on Space Planning, (member, 2008-present).

Dean of Medicine’s Search Committee for Chair of Medical Biophysics (member, 2007-2008).

Dean of Medicine’s Task Force on Faculty Data (member, 2004-2005).

Medical Student Research Day (poster judge, 2003-present).

Regenerative Medicine Executive Committee (member, 2002-present).

Life Science Planning Curriculum Committee (member, 2002-present).

Dean of Medicine’s Task Force on Undergraduate Medical Education (member 2001-2002).

Dean of Medicine’s Task Force on Basic Medical Science Teaching in Arts and Science (member 2001-2002).

Dean of Medicine`s Task Force on promotion and tenure (member 2001-2001).

University of Toronto Blue Sky Canada Research Chair in Women’s Health Search Committee (member, 2004-2006).

Regenerative Medicine Training Program Executive Committee (member, 2002-present).

University of Toronto Multi-Organ Transplant Executive Committee (member, 2001-2009).

McGill University Faculty of Medicine Interdepartmental Flow Cytometry Facility (Director, 1994-2001).

Lyman Duff Building Interdepartmental Animal Facility (Director, 1997-2001).

Dean of Medicine’s Advisory Committee on Canada Research Chairs (2000-2001).

Dean of Medicine's Advisory Committee for Promotion and Tenure (member, 1998-2001).

Dean of Medicine's Search committee for Chair of Microbiology and Immunology (member, 1993-1994).

Dean of Medicine's Search committee for Chair of Microbiology and Immunology (member, 1999).

7

Faculty of Medicine Postgraduate Awards Committee (member, 1998-2001).

Dean of Medicine's committee for coordination of Undergraduate Immunology programs (Chair, 1995-1996).

Faculty of Medicine Animal Care Committee (member, 1997-1999; Chair, 2000).

University Animal Care Committee (member 2000).

Research Executive Committee (member, 2005-2006)Molecular and Cell Biology Search Committee (member, 2001-2003)Molecular and Cell Biology Search Committee (member 2004-present)

Workshop co-ordinator and organizing committee member for the 4th Canadian Society for Immunology Meeting, March 1990, Mont-Gabriel, Quebec.

Organizer of the 6th Canadian Society for Immunology Meeting, March 1992, Mont-Gabriel, Quebec.

Organizer of the 8th Canadian Society for Immunology Meeting, March 1994, Sainte-Adèle, Quebec.

Organizer of the 10th Canadian Society for Immunology Meeting, March 1996, Sainte-Adèle, Quebec.

Organizer of the 12th Canadian Society for Immunology Meeting, March 1998, Sainte-Adeèle, Quebec.

Chairman of the Scientific Program committee for the 14th Canadian Society for Immunology Meeting, March 2000, Chateau Bromont, Quebec.

Chairman of the Scientific Program Committee for the 15th Canadian Society for Immunology Meeting, April 2001, Lake Louise, Alberta.

Member of the Scientific Program Committee for the 16th Canadian Society for Immunology Meeting, April 2002, Blue Mountain, Ontario.

Member of the Scientific Program Committee for the 17th Canadian Society for Immunology Meeting, April 2003, Lake Louise, Alberta.

Member of the Scientific Program Committee for the 18th Canadian Society for Immunology Meeting, April 2005., Whistler, British Columbia.

Chair of the 4th CFBS Northern Lights Symposium on Infection and Immunity, October 2006, Ottawa, Ontario.

Health Canada Scientific Advisory Committee for HIV Therapies (member, 2003-2009).

4.6 Sunnybrook Health Sciences Center Committees

4.7 National committees

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Health Canada Scientific Advisory Committee for Infectious Disease Therapies (member 2009-present).

Stemnet National Center of Excellence Training Awards Committee (member 2009-present).

Convening chairman and organizer of the 1st International Avian CD Nomenclature Workshop. Montreal, June 1991.

Convening chairman of the Avian CD Nomenclature committee of the International Union of Immunology Societies (1991-2001).

Member of the Scientific Program Committee for the 12th International Congress of Immunology, July 2004, Montreal, Quebec.

Vice-Chair for the 7th International Veterinary Immunology Symposium, July 2004, Quebec City, Quebec.

Member of the General Assembly: International Union of Immunology Societies: 2004-2010.

4.8 International committees

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C. Academic History

1. Research Outline

My laboratory has a long standing interest in defining the molecular processes that regulate the development of the B lymphocyte lineage, an interest which originated during my graduate studies in London, U.K. Funding for this work from the Canadian MRC and subsequently CIHR has been continuous since my establishment at McGill University.

The model system we use is the developing chick embryo. This model offers a number of advantages as compared to more widely used murine systems while at the same time yielding insights that are relevant to all higher organisms including humans.

The accessibility of the chick embryo to observation and experimental manipulation is unmatched by any mammalian species. This aspect is well acknowledged by developmental biologists, embryologists and neurobiologists who have extensive studied the chick embryo over the last century. This accessibility extends to the immune system where we can modify the initial stages of lymphoid lineage development using approaches that are not feasible with currently available technology in mammalian species.

B lymphocyte development in birds occurs in a gut associated lymphoid organ, the bursa of Fabricius which has many of the properties associated with the gut associated Peyer's patches and appendix of mammalian species. Although mammalian B cell development in the bone marrow has been extensively studied, the relative contributions of the bone marrow and gut associated lymphoid tissue as a source of developing lymphocytes is currently unknown. This is largely a consequence of the technical difficulties intrinsic to working with mammalian gut associated lymphoid tissue and has resulted in substantial gaps in our understanding of the immune system as a whole. In contrast, the avian bursa, unlike mammalian Peyer's patches, is easy to experimentally remove or manipulate and we have developed over the last 10 years a substantial array of experimental tools to accomplish this.

We have invested considerable time in developing retrovirally mediated gene transfer as an experimental tool to address questions of lymphocyte development in the chick embryo. Conventional mammalian transgenics animals are labor intensive, time consuming and costly to generate. Similarly, the use of retrovirally mediated gene transfer in mammalian models has proved difficult due to severe safety restrictions on the use of productive vectors and the less than ideal efficiency of non-productive vectors. In contrast, avian retroviruses do not infect mammalian cells and so productive avian retroviral vectors can be generated safely under standard laboratory conditions. The inoculation of chick embryos with productive avian retroviruses, engineered to contain genes of interest, leads to rapid dissemination of the virus throughout the embryo and widespread expression of the gene of interest. The biological consequences of such expression can typically be assessed shortly after hatch without requiringbreeding programs to "fix" an inherited transgene. This technology makes it feasible to assess rapidly and effectively the biological properties of families of molecules, or families of site-directed mutants of a given molecule without having to generate large numbers of independent transgenic lines. The application of this technology is just beginning. I foresee that

Experimental systems

in vivo

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over the next five to ten years these applications will expand enormously as investigators, nationally and internationally, understand the benefits of the system.

We have demonstrated that the initial stages of B cell development, involving productive colonization of lymphoid follicles, can be driven by the expression of a truncated immunoglobulin (Ig) receptor which lacks the capacity to bind to antigen. This provides the first example of B cells from an otherwise normal animal in which early B development can be formally dissociated from antigen binding. We have cloned the avian homologues to the Ig receptor associated chains, Ig and Ig , responsible for signal transduction through the surface Ig receptor and by molecular modeling have identified probably sites of inter-molecular assembly of the intact receptor complex. Using the approach of retroviral gene transfer we will be able, by site directed mutagenesis, to dissect the receptor complex at a molecular level to determine amino acid motifs critical for normal B cell development and gain insight into which signal transduction pathways are operative at this stage.

Expression of the truncated Ig receptor is not sufficient to drive the later stages of B cell development that involve cellular redistribution within lymphoid follicles and emigration to the periphery. This provides the first evidence that interaction with antigen may be required to drive the development of B cells in a gut associated lymphoid organ. We will test this directlyusing chimeric surface Ig receptors of defined specificity, introduced by retroviral gene transfer. Should interaction with antigen prove to be required for the later stages of B cell development in these circumstances, it would provide the first example of antigen driven B cell clonal expansion in a primary gut associated lymphoid organ.

During B lymphocyte development in the bursa Ig V genes undergo somatic diversification be gene conversion. This powerful mechanism for generating diversity has been observed in mammalian B cells, including mouse and human, but remains best characterized in the chicken. We have recently demonstrated that in addition to gene conversion which represents a transfer of genetic material from donor pseudogene sequences into a functional V region gene, somatic hypermutation, or point mutations are observed in the bursa. This is surprising since the bursa is a primary lymphoid organ and somatic hypermutation is considered to be a reflection of antigen driven immune responses in secondary tissues. Nonetheless our results imply a role for antigen in bursal B cell development and suggest the possibility that, even in a gut associated primary lymphoid organ, antigen may drive somatic hypermutation. Critically, however, the remarkable correlation between sites of hypermutation in the V gene sequence and the borders of gene conversion events leads us to propose that the molecular process of gene conversion is triggered by somatic hypermutation. We will address this possibility directly by identifying reaction intermediates in the pathway of gene conversion. In addition, we will use retroviral gene transfer to introduce test substrates for gene conversion into developing B cells . Such substrates will be modified to include or exclude defined sites of hypermutation that will allow us to formally identify any causal relationship between somatic hypermutation and gene conversion.

a) The analysis of early B cell development

in vivo

b) The analysis of later stage B cell development

c) Molecular mechanisms of antibody diversification

in vivo

Cell transformation and cell death induced in vivo by regulated oncogene expression

α β

d)

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We have recently succeeded in using retroviral gene transfer to introduce a potent B cell tropic oncogene, , into developing chick embryos under conditions where the expression of the oncogene can be regulated by tetracycline. Although established with the oncogene, this system is applicable to other transforming genes. With respect to , however, we have set up the system to address the question of whether dependent transformation is limited by the expression of other cellular genes. We predicted that if co-expression of other cellular genes was required for mediated transformation, then different stages of B cell development would be differentially susceptible to expression. By allowing B celldevelopment to occur under conditions where is inactive, we can generate individual animals in which all stages of B cell development are established normally. When expression is induced in these animals, there is massive and polyclonal induction of peripheral B cell tumors. Remarkably, however, the immature B cells in the bursa die by apoptotic cell death. This formally demonstrates that the consequences of expression are defined by the expression of cellular genes and that depending on the spectrum of genes expressed expression leads to oncogenic transformation of cell death. We are therefore working towards defining the nature of those genes that determine the consequences of expression. Critically, however, this system provides a model in which the fate of transformed cells might be modified, not by modulating oncogene expression, but by defining and modulating normal cellular genes whose expression is required for transformation.

2. Research Awards

1976-1980 Wellcome studentship.1983-1986 Imperial Cancer Research Fund Research Post-doctoral Fellowship.1993-1996 Fonds de la Recherche en Santé du Québec Chercheur-boursier (Senior 2).1990-1993 Fonds de la Recherche en Santé du Québec Chercheur-boursier (Senior 1).1987-1990 Fonds de la Recherche en Santé du Québec Chercheur-boursier (Junior 2).

1987-1989 Medical Research Council of Canada. Project entitled "Control of B Lymphocyte Ontogeny". $68,000 p.a. plus $80,000 equipment.

1989-1992 Medical Research Council of Canada. Project entitled "Control of B Lymphocyte Ontogeny". $70,800 p.a.

1990-1993 National Cancer Institute of Canada. Project entitled "Retroviral transformation of the B Lymphocyte Lineage". $67,894 p.a. plus $8,000 equipment.

1992-1995 Medical Research Council of Canada. Project entitled "Control of B Lymphocyte Ontogeny". $80,612 p.a.

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Graduate:Postdoctoral:

Faculty:

Operating grants:

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1993-1996 National Cancer Institute of Canada. Project entitled "Retroviral transformation of lymphocytes by the v- oncogene". $76,465 p.a. plus $8,704 equipment.

1996-1997 National Cancer Institute of Canada. Project entitled "Retroviral transformation of lymphocytes by the v- oncogene". $55,889 p.a.

1995-2000 Medical Research Council of Canada. Project entitled "Control of B lymphocyte Ontogeny". $78,878 p.a. plus a studentship.

2000-2005 Canadian Institutes of Health Research. Project entitled "Control of B lymphocyte Ontogeny". $142,867 p.a. plus $14,634 equipment.

2005-2010 Canadian Institutes of Health Research. Project entitled "Control of B lymphocyte Ontogeny". $152,000 p.a.

1987 Faculty of Graduate Studies and Research, McGill University. Cryopreservation freezer. $9,535.00.

1988 Medical Research Council of Canada. Group grant, Drs. Antel (P.I.), Julius, Ratcliffe and Loescher. Gammacell-1000 blood irradiator. $50,265.00.

1991 Medical Research Council of Canada. Group grant, Drs. Ratcliffe (P.I.), Julius and Osmond. FACScan, Becton-Dickenson Flow Cyto-meter. $61,380.00.

1991 National Cancer Institute of Canada. Group grant, Drs. Ratcliffe (P.I.), Julius and Osmond. FACScan, Becton-Dickenson Flow Cyto-meter. $60,107.00.

1994 Medical Research Council of Canada. Group grant, Drs. Ratcliffe (P.I.) et al. FACS Vantage (Becton Dickinson cell sorter). $175,000.00.

1995-2000 Medical Research Council of Canada. Flow cytometry facility maintenance. Group grant; Drs Ratcliffe (P.I.) et al. $47,500 p.a.

2000-2005 Canadian Institutes of Health Research. Flow cytometry facility maintenance. Group grant; Drs Ratcliffe (P.I.) et al. $60,000 p.a. plus $59,859 equipment.

2003-2004 Canadian Institutes for Health Research. Center for Cytometry and Scanning Microscopy. Group grant; Dr. J.C. Zuniga-Pflucker (P.I.) et al. $108,749 p.a.

rel

rel

Equipment grants:

Equipment maintenance grants:

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2005-2010 Canadian Institutes of Health Research. Center for Cytometry and Scanning Microscopy. Group grant; Dr. J.C. Zuniga-Pflucker (P.I.) et al. $91,838 p.a.

2002-2009 Canadian Institutes of Health Research. Training grant in Regenerative Medicine. Group grant. Dr. G. Levy (P.I.) et al. $300,000 p.a.

2009-2015 Canadian Institutes of Health Research. Training grant in Regenerative Medicine. Group grant. Dr. G. Levy (P.I.) et al. $325,000 p.a.

and Ivanyi, J. Allelic exclusion of the surface expression of surface IgM allotypes on spleen and bursal B cells in the chicken. 1979. 149-156.

and Ivanyi, J. Allotype suppression in the chicken. I. Generation of chronic suppression in heterozygous but not in homozygous chickens.

1979. 847-852

and Ivanyi, J. Allotype suppression in the chicken. II. Suppression in homozygous chickens with anti-allotype antibody and allotype disparate B cells.

1981. 296-300.

and Ivanyi, J. Allotype suppression in the chicken. III Analysis of the recovery from suppression by neonatally injected or maternally derived antibodies.

1981. 301-306.

and Ivanyi, J. Allotype suppression in the chicken. IV. Deletion of B cells and lack of suppressor cells during chronic suppression. 1981.

306-310.

and Julius, M.H. H-2 restricted T-B cell interactions involved in polyspecific B cell responses mediated by soluble antigen. 1982.

643-641.

and Julius, M.H. Two classes of bystander B cell response: activation requirements reflect those of B cells in general. 1983. 581-586.

Training grants:

Refereed Publications

Immunogenetics.

Eur. J. Immunol.

Eur. J. Immunol.

Eur. J. Immunol.

Eur. J. Immunol.

Eur. J. Immunol.

J. Immunol.

D. Publications

1. Ratcliffe, M.J.H.9:

2. Ratcliffe, M.J.H.

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Allotype suppression in the chicken. V. Abnormal ratios of chronically suppressed IgM and IgG isotypes. 1984. 208-214.

Vainio, O. and Proliferation of chicken peripheral blood leukocytes in response to pokeweed mitogen is macrophage dependent. 1984.

225-243.

and Julius, M.H. T dependent activation of resting B cells mediated by concanavalin A. 1984. 280-283.

, Julius, M.H. and Kim, K.-J. Heterogeneity in the response of T cells to antigen presented by B lymphoma cells. 1984. 49-60.

Orme, I.M., and Collins, F.M. Acquired immunity to heavy infection with BCG and its relationship to the development of non-specific unresponsiveness 1984. 285-296.

and Mitchison, N.A. Function of Ig receptors in B cell triggering. 1984. : 73-79, 105-106.

Owens, T., Czitrom, A.A., Gascoigne, N.R.J., Crispe, I.N., , Lai, P.K. and Mitchison, N.A. The presentation of cell surface alloantigens to T cells. 1984. 189-201.

Pink, J.R.L., and Vainio, O. Immunoglobulin bearing stem cells for clones of B (bursa derived) lymphocytes. 1985. 617-620.

Ontogeny and cloning of B cells in the bursa of Fabricius. 1985. 223-227.

Lack of pre-B cells in hatched chickens. 1985. 349-350.

Vainio, O., and Leanderson, T. Chicken T cell growth factor: use in the generation of long-term cultured T cell lines and biochemical characterisation.

1986. 135-142.

, Lassila, O., Pink, J.R.L. and Vainio, O. Avian B cell precursors: cell surface immunoglobulin expression is an early, possibly bursa independent event.

1986. 129-133.

, Lassila, O., Reynolds, J., Pink, J.R.L. and Vainio, O. A re-evaluation of the function of the bursa of Fabricius. 1987. : 3-14.

Mitchison, N.A. and B cells can be activated in more than one way. in , Levy, J.G. and Nepom, G.T. Eds. CRC Press, 1987. pp. 163-169.

Vainio, O., Veromaa, T., Eerola, E., Toivanen, P. and Antigen-presenting cell-T cell interaction in the chicken is MHC class II restricted.1988. 2864-2868.

Cell. Immunol.

Cell. Immunol.

Eur. J. Immunol.

Cell. Immunol.

mycobacterium bovis in vitro. Cell. Immunol.

Ann. Immunol. (Inst. Pasteur) Paris.

Immunobiol.

Eur. J. Immunol.

Immunol. Today

Immunol. Today

Scand. J. Immunol.

Eur. J. Immunol.

Prog. Clin. Biol. Res.

Paradoxes in Immunology

J. Immunol.

15


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Julius, M.H., Rammensee, H.-G., , Lamers, M.C., Langhorne, J. and Kohler, G. The molecular interactions with helper T cells which limit antigen specific B cell differentiation. 1988. 381-386.

Julius, M.H., Rammensee, H.-G., Lamers, R., Langhorne, J. and Kohler, G. Why cognate Th-B interaction usually limits the activation of resting B cells. in Davis, M.M. and Kappler, J., Eds. Alan R. Liss Inc. 1988. pp. 217-227.

and Klaus, G.G.B. The biology of B cell activation. in Bird, G. and Calvert, J. Eds. Oxford University Press. 1988. pp.

102-129.

and Lamb, J.R. Lymphocyte purification, growth, cloning and functional assays. in

Moorley, J. and Bray, M.A. Eds. Springer-Verlag A.G. 1988. 319-343.

Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius. 1989. 1165-1173.

Development of the avian B lymphocyte lineage. 1989. 207-234.

Huffnagle, G., and Humphries, E.H. Bu-2, a cell surface antigen present on distinct avian hematopoetic lineages, is expressed in germinal centers.

1989. 589-604.

and Tkalec, L. Cross-linking of the surface immunoglobulin of lymphocytes from the bursa of Fabricius results in second messenger generation.

1990. 1073-1078.

and Paramithiotis, E. The end can justify the means. 1990. 217-226.

(Ed.) B Lymphocyte Development. 1990. 165-226.

Veillette, A. and Avian CD4 and CD8 interact with a cellular tyrosine

protein kinase homologous to mammalian p56 1991. 397-401.

Benatar, T., Iacampo, S., Tkalec, L. and Expression of immunoglobulin genes in the avian embryo bone marrow revealed by retroviral transformation. 1991. 2529-2536.

Paramithiotis, E., Tkalec, L. and High levels of CD45 are expressed co-ordinately with CD4 and CD8 on avian thymocytes. 1991. 3710-3717.

Eur. J. Immunol.

The T Cell Receptor,

B Lymphocytes in Human Diseases,

Pharmacology of Lymphocytes. Handbook of Experimental Pharmacology,

J. Exp. Med.

CRC Crit. Rev. Poult. Biol.

Hybridoma

Eur. J. Immunol.

Semin. Immunol.

Semin. Immunol.

lck. Eur. J. Immunol.

Eur. J. Immunol.

J. Immunol.

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, Coggeshall, K.M., Newell, M.K. and Julius, M.H. T cell receptoroligomerisation but not dimerisation, induces increased cytosolic calcium concen-trations and reveals a lack of stable association between CD4 and the T cell receptor complex. 1992. 1643-1651.

Srikumar, R., Cain, A.C., Vachon, V., Richardson, C.R., , Saarinen, L., Käyhty, H., Mäkelä, P.H. and Coulton, J.W. Monoclonal antibodies specific to porin of type b: localization of their cognate epitopes and tests of their biological activities. 1992. 665-676.

Chow, L.M.C., and Veillette, A. is the avian homologue of the mammalian tyrosine protein kinase gene. 1992. 1226-1233.

Benatar, T., Tkalec, L. and Stocastic rearrangement of immunoglobulin variable region genes in chicken B cell development.

1992. 7615-7619.

Reddy, S.K., Silim, A. and Biological roles of the major capsid proteins and relationships between the two existing serotypes of infectious bursal disease virus. 1992. 209-222.

Srikumar, R., Dahan, D., Gras, M.F., , van Alphen, L. and Coulton, J.W. Antigenic sites on porin of type b: mapping with synthetic peptides and evaluation of structure predictions. 1992. : 4007-4016.

Marmor, M.D., Benatar, T. and Retroviral transformation of chicken T cells expressing either or T cell receptors by reticuloendotheliosis virus strain T. 1993. 647-656.

, Benatar, T., Coumidis, A., Iacampo, S. and Marmor, M.D. Retroviral transformation as an approach to analyzing early stages of chicken B lymphocyte development. 1993. 331-336.

Paramithiotis, E. and Bursa dependent subpopulations of peripheral B lymphocytes in chicken blood. 1993. 96-102.

Reddy, S.K., and Silim, A. Flow cytometric analysis of the neutralizing immune response against infectious bursal disease virus using avian retrovirus-transformed lymphoblastoid cell lines. 1993. 167-178.

Benatar, T. and Polymorphism of the functional immunoglobulin variable region genes in the chicken by exchange of sequence with donor pseudogenes. 1993. 2448-2453.

, Boyd, R., Chen, C.H. and Vainio, O. Avian CD Nomenclature workshops, Montreal, June 1991, Budapest, August 1992 and Tours, September 1992. 1993. 375-386.

Paramithiotis, E. and Bursa dependent and independent peripheral B cell populations in the chicken. 1993. 325-330.

J. Immunol.

Haemophilus influenzaeMol. Microbiol.

tkllck Mol. Cell. Biol.

Proc Natl. Acad. Sci. USA.

Arch. Virol.

Haemophilus influenzaeJ. Bacteriol.

in vitro

J. Exp. Med.

Les colloques de l’I.N.R.A.

Eur. J. Immunol.

J. Virol. Mthds.

Eur. J. Immunol.

Vet. Immunol. Immunopathol.

Les colloques de l’I.N.R.A.

αβ γδ

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176:



181:


154

56.Ratcliffe, M.J.H.

2


177



212



Ligation of cell surface immunoglobulin in the chicken bursa of Fabricius. 1993. 450-455.

Paramithiotis, E. and B cell emigration directly from the cortex of lymphoid follicles in the bursa of Fabricius. 1994. 458-463.

Paramithiotis, E. and Survivors of bursal B cell production and emigration. 1994. 991-997.

Moeck, G.S., Bazzaz, B.S.F., Gras, M.F., Ravi, T.S., and Coulton, J.W. Genetic insertion of a reporter epitope in the ferrichrome-iron receptor of

K-12. 1994. 4250-4259.

and Jacobsen, K. Rearrangement and diversification of immunoglobulin genes in chicken B cell development. 1994. 175-184.

Paramithiotis, E. Jacobsen, K. and Loss of cell surface immunoglobulin expression precedes B cell death by apoptosis in the bursa of Fabricius. 1995. 105-113.

Tregaskes, C.A., Kong, F.K., Paramithiotis, E., Chen, C.L., , Davison, F., and Young, J. Identification and analysis of the expression of CD8 and CD8 isoforms in chickens reveals a major TCR CD8 subset of intestinal intraepithelial lymphocytes. 1995. : 4885-4494.

Chan, S.L., Alizadeh-Khiavi, K., Chronopoulos, S., Li, W., Fried, V., Smith, H.T., Butt, T., and Ali-Kahn, Z. Recombinant and amyloid-associated ubiquitin

during amyloidogenesis. 1995. 191-194.

Moeck, G.S., and Coulton, J.W. Topological analysis of the ferrichrome-iron receptor using monoclonal antibodies.

1995. : 6118-6125.

Jacobsen, K.A., Paramithiotis, E., Ewert, D.L. and Apoptotic cell death in the chicken bursa of Fabricius. In

Gupta, S. and Cohen, J.J. (Eds.), Plenum Publishing Corp., 1996. pp 155-166.

Paramithiotis, E. and Evidence for phenotypic heterogeneity among B cells emigrating from the bursa of Fabricius. A reflection of functional diversity?

1996. : 27-34.

, Paramithiotis, E., Coumidis, A., Sayegh, C.E., Demaries, S.L., Martinez, O. and Jacobsen, K.A. The bursa of Fabricius and its role in avian B lymphocyte development. In , Davison, F., Payne, J. and Morris, T.R. (Eds.), Carfax, 1996. pp11-30.

The study of B cell differentiation. In , Lefkovits, I. (Ed), Academic Press, 1997. pp 2200-2211.

Res. Immunol.

Eur. J. Immunol.

Poult Sci.

Escherichia coli J. Bacteriol.

Semin. Immunol.

J. Exp. Med.

J. Immunol.

in vivo Int. J. Clin. Invest.

Escherichia coli J. Bacteriol.

Mechanisms of Lymphocyte Activation and Immune Regulation VI. Cell Cycle and Programmed Cell Death in the Immune System.

Curr. Top. Microbiol. Immunol.

Poultry Immunology

Manual of Immunological Methods, 2nd Edition

αα αβ γδ αα

18






96


18

68. Ratcliffe, M.J.H.72


175


164



1414

1414

Chicken immunoglobulin isotypes and allotypes. In , Weir, D.W., Herzenberg, L.A. and Herzenberg,

L.A. (Eds), Blackwell Scientific Publications, Oxford, 1997. pp 24.1-24.15.

Jeurissen, S.H.M., Vainio, O. and Leukocyte markers in the chicken. In , Pastoret, P.P., Griebel, P. and Govaerts, H. (Eds.), Academic Press Ltd., London. 1998. pp81-85.

Demaries, S.L. and Cell surface and secreted immunoglobulins in chicken B cell development. In , Pastoret, P.P., Griebel, P. and Govaerts, H. (Eds.), Academic Press Ltd., London. 1998. pp89-92.

Sayegh, C.E., Drury, G.L., Demaries, S.L., Iacampo, S. and Colonization of the bursa of Fabricius by sIg+ B cell precursors lacking V(D)J encoded determinants: a clonal analysis. In , Talwar, G.P., Nath, I., Ganguly, N.K. and Rao, K.V.S. (Eds.), Monduzzi Editore, Bologna, Italy. 1998. pp 111-114.

Sayegh, C.E., Demaries, S.L., Iacampo, S. and Development of B cells that lack V(D)J-encoded determinants in the avian embryo bursa of Fabricius.

. 1999. : 10806-10811.

Sayegh, C.E., Drury, G.L. and Efficient antibody diversification by gene conversion in the absence of selection for V(D)J-encoded determinants.

1999. : 6319-6328

Sayegh, C.E., Rao, M.A. and Avian B cell development: lessons from transgenic models. 1999. : 31-37.

Sayegh, C.E., Demaries, S.L., Friedmann, J., Pike, K. and The chicken B cell receptor complex and its role in avian B cell development.2000. : 187-200.

Sayegh, C.E. and Perinatal deletion of B cells expressing surface immunoglobulin molecules that lack V(D)J-encoded determinants in the bursa of Fabricius is not due to intra-follicular competition. 2000. : 5041-5048.

Pike, K.A., Friedmann, J.E., Iacampo, S., Sandhu, R. K. and Replacement of surface immunoglobulin expression by the cytoplasmic domains of the Ig heterodimer in early B cell development. In

Schat, T., Dietert, R., Marsh, J. and Naqi, S. (Eds.), AAAP Press. 2001. 90-97.

and Pike, K.A. Influence of antibody diversification on the mechanism of haplotype exclusion of immunoglobulin gene expression.

2002. : 199-205. Second round comments on the issue of haplotype exclusion. : 233. Final round comments on the issue of haplotype exclusion.

: 243-244. Final final round comments on the issue of haplotype exclusion. : 245.

Handbook of Experimental Immunology, 5th edition

Handbook of Vertebrate Immunology

Handbook of Vertebrate Immunology

10th International Congress of Immunology

Proc. Natl. Acad. Sci. USA

in vivoEMBO J. .


Immunol. Rev.

J. Immunol.

Proceedings of the 6th AIRG Meeting,

Semin. Immunol.

ibidibidibid

α/β

19

73. Ratcliffe, M.J.H. 14


75. Ratcliffe, M.J.H. 197


172


174:


108:




284:

82.Ratcliffe, M.J.H.,

8:





Pike, K.A. and Cell surface immunoglobulin receptors in B cell development. Semin. Immunol. 2002. : 351-358.

B cell development in gut associated lymphoid tissues. 2002. 337-341.

Pike, K.A., Baig, E. and Distinct roles for Ig and Ig in chicken B cell development. 2004. : 10-15.

Pike, K.A., Iacampo, S., Friedmann, J.E. and The cytoplasmic domain of Ig is necessary and sufficient to support efficient early B cell development.

2004. : 2210-2218.

Pike, K.A. and Dual requirement for the Ig immunoreceptor tyrosine-based activation motif (ITAM) and a conserved non-Ig ITAM tyrosine in supporting Ig -mediated B cell development. 2005. 2012-2020.

Aliahmad, P., Pike, K.A. and Cell surface immunoglobulin regulated check-points in chicken B cell development. 2005.

3-9.

Antibodies, immunoglobulin genes and the bursa of Fabricius in chicken B cell development. 2006. 101-118.

Pike, K.A. and Ligand-independent signaling during early avian B cell development. 2006. 103-116.

Imamura, Y., Oda, A., Katahira, T., Bundo, K., Pike, K.A., and Kitamura, D. BLNK binds active H-Ras to promote B cell receptor-mediated capping and ERK activation. 2009. 9804-9813.

Chan, E.T., Quon, G.T., Chua, G., Babak, T., Trochesset, M., Zirngibl, R.A., Aubin. J., Wilde, A., Brudno, M., Morris, Q.D. and Hughes, T.R. Conservation

of core gene expression in vertebrate tissues. 2009. 33-33.17.

Kothlow, S., Schenk-Weibhauser, K., and Kaspers, B. The RCAS retroviral gene transfer technology: an efficient system for functional studies with soluble proteins in the chicken.

Akram, A., Williams, D., Slutsky, A.S. and DosSantos, C.C. Pattern of HLA alele co-expression influences immunodominance of anti-influenza CTL responses.

Agard, E., Dervovic, J., Chou, M.-Y., Lewis, S. and Frequent DH to DJHrearrangement at the chicken immunoglobulin heavy chain locus mediated by efficient spacer sequence dependent RSS12/12 recombination. .

Neschadim, A. and A high frequency of immunoglobulin heavy and light chain combinations isolated from the chicken bursa of Fabricius fail to pair.

.


Immunol. Rev.

J. Immunol.

J. Immunol.


Dev. Comp. Immunol.

Immunol Res.

J. Biol. Chem.

J Biol.

in vivoSubmitted for publication.

Sunbitted for publication.

in preparation

In preparation

α β

α

αα

αβ

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E. Presentations and Special Lectures

National and International Symposia and Workshop Chairmanships:

International Union of Immunology Societies 6th International Immunology Congress,

6th Canadian Society for Immunology Meeting, Mont Gabriel,

3rd

International Veterinary Immunology Symposium,

3rd International Veterinary Immunology Symposium, Budapest,

Avian Immunology Research Group Meeting,

Avian Immunology Research Group Meeting, Tours, France,

7th Canadian Society for Immunology Meeting,

Avian Immunology Symposium,



World Poultry Science Association Meeting,

American Society of Zoologists Meeting

International Symposium on the Challenges of Lower Vertebrates to Immunology


5th International Veterinary Immunology Symposium,

14th Canadian Society for Immunology Meeting

15th Canadian Society for Immunology Meeting

Workshop chairman (B cell subpopulations). Toronto 1986.

Symposium co-chairman (Immunoglobulin genes). March 1992.

Workshop chairman (2nd International Avian CD Nomenclature Workshop). Budapest, August 1992.

Symposium chairman (Developmental and Comparative Immunology). Hungary, August 1992.

Workshop chairman (3rd International Avian CD Nomenclature Workshop). Tours, France, September 1992.

Symposium co-chairman (Ontogeny and lymphoid development). September 1992.

Workshop chairman (Antigen expression in lymphocyte development). Lake Louise, Alberta, March 1993.

Workshop chairman (4th International Avian CD Nomenclature Workshop). East Lansing, July 1993.

Symposium co-chairman (B cell development). Reading, U.K., March 1994.

Workshop chairman (5th International Avian CD Nomenclature Workshop). Compton, U.K., March 1994.

Symposium chairman (T lymphocyte development) Reading, U.K. September 1995.

Symposium co-chairman (Avian Biology: The Immune System and Environmental Stressors) , Washington, U.S.A., December 1995.

Symposium co-chairman (Evolutionary Aspects of the Immune System) , Kyoto,

Japan, November 1997.

Workshop chairman (6th International Avian CD Nomenclature Workshop). Turku, Finland, June 1998.

Symposium Chairman (Avian Immunology) Ludhiana, India, November 1998.

Workshop co-chairman (Lymphocyte development). , Chateau Bromont, Quebec, March 2000.

Symposium Chairman (B cells in secondary lymphoid organs) , Lake Louise, Alberta, April 2001.

21

Symposium Chairman (B cells and Antibodies) Uppsala, Sweden, July 2001.

Symposium co-chair (Bursal Physiology and Oncogenesis) , Seattle, Washington, USA,

October 2002.

Minisymposium Co-chair (Mechanisms of B Cell Diversity) . Montreal, Que. July 2004.

Minisymposium Co-chair (Evolution of the Immune System) . Rio de Janeiro, Brazil, August 2007.

British Society for Immunology Summer Meeting, Bristol, UK, July 1979.

International Conference on Avian Immunology, Philadelphia, USA, July 1986.

4th Canadian Society for Immunology Meeting, Mont-Gabriel, March 1990.

6th Canadian Society for Immunology Meeting, Mont-Gabriel, March 1992.

American Veterinary Medicine Association Annual Meeting, Boston, USA, August 1992.

3rd International Veterinary Immunology Symposium, Budapest, Hungary, August 1992.

Avian Immunology Research Group Meeting, Tours, France, September 1992.

Avian Immunology Symposium, East Lansing, USA, July 1993.

Avian Immunology Research Group Meeting, Reading, U.K., March 1994.

Immunology and Developmental Biology of the Chicken, Basel, Switzerland, December 1994.

World Poultry Science Association Meeting, Reading, U.K., September 1995.

American Society of Zoologists, Washington, U.S.A., December 1995.

6th International Veterinary Immunology Symposium,

International Workshop on Experimental Models from the Bursa of Fabricius



National and International Symposium Presentations:

Allotype suppression in the chicken.

B lymph-ocyte ontogeny in the chicken.

Generation of B cells and Immunoglobulin diversity in the avian bursa of Fabricius.

Chicken Ig genes and concluding remarks.

High frequency retroviral transformation of chicken T cells expressing either ???or ?? T cell receptors.

Early B cell development in the chicken.

Allelic variation of chicken immunoglobulin genes and the sequence of their rearrange-ment.

Bursal and post-bursal B cell development in the chicken.

Life and death in chicken B cell development.

Life and death in the B cell lineage.

Regulation of B lymphocyte development.

Regulation of lymphoid development and activation.

22

6th International Conference on Lymphocyte Activation and Immune Regulation, Newport Beach, U.S.A., February 1996.

International Symposium on the Challenges of Lower Vertebrates to Immunology, Kyoto, Japan, November 1997.

5th Avian Immunology Research group Meeting, Turku, Finland, June 1998.

5th International Veterinary Immunology Symposium, Ludhiana, India, November 1998.

6th Annual Congress of the British Society for Immunology, Harrogate, U.K. December 1998.

15th Canadian Society for Immunology Meeting, Lake Louise, Alberta, April 2001.

6th International Veterinary Immunology Symposium, Uppsala, Sweden, July 2001.

AAVI-ACVM 2001 Symposium, St. Louis, Missouri, USA, November 2001.

International Workshop on Experimental Models from the Bursa of Fabricius, Seattle, Washington, USA. October 2002.

12th International Congress of Immunology, Montreal, Que. July 2004. (Minisymposium presentation).

7th International Veterinary Immunology Symposium, Quebec City, Que. July 2004.

13th International Congress of Immunology, Rio de Janeiro, Brazil, August 2007.

Apoptotic cell death and its regulation in the avian B lymphoid system.

Avian B lymphocyte development and the bursa of Fabricius.

No obligatory ligand for V(D)J encoded determinants of the pre-diversified B cell surface immunoglobulin receptor.

Avian B cell development: lessons from transgenic models.

Transgenic regulation of avian B cell development.

Evolution of lymphopoiesis from a secondary lymphoid organ.

Membrane proximal expression of the cytoplasmic domain of Ig is sufficient to drive B cell development.

The role of the surface immunoglobulin receptor complex in chicken B cell development.

Basal signaling through the cytoplasmic domain of Ig is sufficient to support early B cell development.

Diverse mechanisms of antibody diversity.

Chicken B cell development: a paradigm for the generation of B cell repertoires in the gut.

Evolution of primary B cell lymphopoeisis from a secondary lymphoid organ.

a

a

23

Selected Invited seminars and lectures (1990-present):

Avian B cell development.

How birds do it!


Immunoglobulin genes in avian B cell development.







Normal and transformed models of avian B lymphocyte development.

Normal and Transformed Models of avian B lymphocyte development.

B cell development: how birds do it.

Life and death in theavian bursa of Fabricius.






Normal and transgenic models of chicken B cell development.

Department of Immunology, Rush Presbyterian Hospital, University of Chicago. March 1990.

Center for Host Resistance, McGill University. March 1991.

Department of Anatomy, McGill University. November 1991.

Department of Biochemistry, University of Sherbrooke. February 1992.

Department of Immunology, University of Toronto. February 1992.

Department of Immunology, University of Glasgow, U.K. April 1992.

Department of Immunology, The Medical School, Newcastle upon Tyne, U.K. April 1992.

Institute for Animal Health, Compton, U.K. April 1992.

Department of Pathology, University of Cambridge, U.K. April 1992.

Central Research Division, Pfizer Inc., Groton, CT. May 1992.

Department of Microbiology, State University of New York at Stony Brook. November 1992.

USDA-RPRL, East Lansing, MI. December 1992.

Department of Medical Physiology, University of Calgary. March 1993.

Department of Avian Sciences, Cornell University. November 1993.

Montreal General Hospital Research Institute, McGill University. February 1994.

Basel Institute for Immunology, Basel, Switzerland, December 1994.

Max Plank Institute for Immunobiology, Frieburg, Germany, December 1994.

Institute for Genetics, Cologne, Germany, December 1994.

School of Biological Sciences, University of Manchester, Manchester, U.K., September 1995.

Central Research Division, Pfizer Inc., Groton, CT, U.S.A., December 1995.

24

Department of Immunology, University of Toronto, January 1996.

City of Hope Medical Center, Duarte, California, U.S.A., February 1996.

Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, U.S.A., February 1996.

Department of Anatomy, McGill University, Montreal, February 1996.

Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., June 1996.

Department of Medical Microbiology, University of Calgary, Calgary, Alta., May 1996.

Department of Microbiology and Immunology, University of Western Ontario, London, Ont., November 1996.

Department of Immunology, University of Toronto, Toronto, Ont., February 1997.

Department of Microbiology and Immunology, University of Sherbrooke, Sherbrooke, Que., April 1997.

Department of Immunology, University of Tokyo, Tokyo, Japan, November 1997.

Department of Pathology, CMU, University of Geneva, Geneva, Switzerland, June 1998.

Basel Institute for Immunology, Basel, Switzerland, June 1998.

University Pierre and Marie Curie, Paris, France, July 1998.

Department of Immunology, University College London, London, U.K., July 1998.

Department of Immunology, University of Glasgow, Glasgow, U.K., July 1998.

Montreal Children's Hospital research Institute, Montreal, Que., November 1998.

Institute for Animal Health, Compton, U.K. December 1998.

Department of Immunology, University of Toronto, Toronto, Ont., November 1999.

Department of Immunology, University of Toronto, Toronto, Ont., May 2000.


Avian B lymphocyte development.

Life and death in avian B cell development.

Avian B lymphocyte development.

Life and death in avian B cell development.

Development of avian B lymphocytes.

Development of B lymphocyte lineages.

Development of chicken B lymphocyte lineages.

Development of chicken B lymphocyte lineages.

Regulation of apoptosis in avian B cell development.


Avian B lymphocyte development regulated by retroviral gene transfer.


Avian B cell development regulated by retroviral gene transfer.

Avian B cell development regulated by retroviral gene transfer.

Trans-genic regulation of avian B lymphocyte development.

Cell surface immunoglobulin in chicken B cell development.

The chicken sIg receptor complex and its role in B lineage development.

The role of the surface immunoglobulin receptor complex in B lymphocyte development.

25

Department of Microbiology, Loyola University, Chicago, IL, USA. September 2000.

Department of Anatomy and Cell Biology, University of Calgary, Calgary, Alta. April 2001.

Heinrich-Pette-Institute, Hamburg, Germany, July 2001.

Department of Pathobiology, University of Guelph, Guelph, Ont., November 2001.

CIHR Transplantation Seminar Series, University of Toronto, Toronto, Ont., November 2001.

Department of Microbiology and Immunology, Université de Montréal, Que. January 2002.

Biotechnology Research Center, University of British Columbia, Vancouver, BC, October 2002.

Albert Einstein College of Medicine, New York, NY, USA. September 2003.

Origen Therapeutics, Burlingame, CA, USA. March 2004.

Michigan State University, East Lansing, MI, USA. May 2005.

Immunology Research Group, University of Calgary, June 2005.

CIHR Transplantation Group, Toronto, Ontario, February 2006. .

Center for Women’s Health Symposium on Emergent Paradigms in Women’s Health, Toronto, Ontario, May 2006. .

Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, July 2009. .


The chicken sIg receptor complex and its role in B lineage development.



B lymphocyte development dissected by retroviral gene transfer.

The role of the surface immunoglobulin receptor complex in B lymphocytedevelopment.

The role of the surface immunoglobulin complex in B lymphocyte development.

Role of surface immunoglobulin in B cell development.

Surface immunoglobulin and B cell development.

Regulation of B cell development by the surface immunoglobulin receptor complex.

Regulation of B cell development by the surface immunoglobulin receptor complex.

Immunoglobulin gene expression and B cell development

The immune system

Regulation of B cell development in gut associated lymphoid tissue

26

F. Teaching and design1986-2001, Department of Microbiology and Immunology, McGill University;2001-present, Department of Immunology, University of Toronto

1. Courses taught

2002-present Immunobiology (IMM335Y) Course coordinator and lecturer, 6 hours. Design and implementation of new course for Immunology Specialists.

2001-present Immunobiology (IMM334Y) Course coordinator and lecturer, 12 hours.

2001-present Recent Advances in Immunology (IMM1016) lecturer, 4 hours.

2003-present Pathobiology of Disease (Undergraduate Medical Curriculum, year 2) lecturer, 1 hour.

2003-present Developmental Immunology (IMM429H) lecturer, 2 hours.

2003-present The Immune Response (IMM430H) lecturer, 2 hours.

2009-present Immunology of Emerging Infectious Diseases, Shantou University, China, lecturer, 12 hours.

1988-2001 Advanced Immunology (528-414A) Course coordinator and lecturer, 8 hours. Design and implementation of new course.

1987-2001 Immunology (528-314B) Course coordinator (1994-2001) and lecturer 12 hours.

1991 Selected Topics in Lymphocyte Development (Graduate Reading and Conference course) Course coordinator.

1995 Cell and Molecular Events in Lymphocyte Development (Graduate Reading and Conference course) Course coordinator.

2001 Recent Advances in Lymphocyte development (Graduate Reading and Conference course) Course coordinator.

1992-2001 Academic Advisor, Department of Microbiology and Immunology.

27

2. Undergraduate student research project supervision

Summer students:

Final Year Research Project Students

Sandra Iacampo (1990)Spencer Lister (1991) Mina Marmor (1991)Mina Marmor (1992)Vasiliki Bitzas (1993)Camil Sayegh (1994)Sandra Demaries (1994)Nathalie Earl (1995)Martin Chang (1995)Sandra Demaries (1995)Benjamin Mathew (1995) Kristie Devreitis (1996)Iris Doyle (1996)Peggy Lau (1996)Gillian Drury (1998)Kelly Pike (1999)Jennifer Friedmann (1999)Krista Heinonen (2000)Roop Kaur Sandhu (2000)Ming-Yi Chou (2002)

Sham Baig (2002)Ephraim Tang (2003)Raj Satkunisavam (2003)Christina Grava (2004)Ania Radziszewska (2004)Gladys Wong (2005)Joseph Fiorelino (2005)Pamela Tsao (2005, 2006)Jennifer Li (2006)Jerome Chan (2006)Michael Orthofer (2006) University Of ViennaDianne Wu (2007)David Shih (2007)Harveer Mihal (2007)Linda Zhao (2008)Christopher Yao (2009)Nhat Tran (2009)Yurij Baglaekno (2009)

Nathalie Riebel (1987-88) Microbiology and Immunology Honors Program.David Wilson (1988-89) Microbiology and Immunology Honors Program.Tania Benatar (1989-90) Microbiology and Immunology Honors Program.

Winner of the E.D.G. Murray Prize for top graduating Honors student.Jennifer Wilshire (1993) Microbiology and Immunology Honors Program.Mina Marmor (1992-93) Interdepartmental Honors Immunology Program.Sandra Demaries (1994-95) Microbiology and Immunology Honors program.Benjamin Matthew (1995-96) Microbiology and Immunology Major Program.Cemaine Tsang (1996) Microbiology and Immunology Major Program.Iris Doyle (1996-97) Microbiology and Immunology Honors Program.Peggy Lau (1996-97) Interdepartmental Honors Immunology Program.Derrick Gibbings (1997-98) Microbiology and Immunology Honors Program.Gillian Drury (1998-1999) Microbiology and Immunology Honors Program.Kelly Pike (1999-2000) Microbiology and Immunology Honors Program.Jennifer Friedmann (1999-2000) Microbiology and Immunology Honors

Program.

28

Krista Heinonen (2000-2001) Microbiology and Immunology Honors Program.Roop Kaur Sandhu (2000-2001) Microbiology and Immunology Honors Program.Sham Baig (2002-2003) Immunology IMM450Y.Husam Abdel-Qadir (2002-2003) Immunology IMM450Y.Ephraiam Tang (2003-2004) Immunology IMM450Y.Raj Satkunisavam (2003-2004) Immunology IMM450Y.Christina Grava (2004-2005) Immunology IMM450Y.Ania Radziszewska (2004-2005) Immunology IMM450Y.Gladys Wong (2005-2006) Immunology IMM450Y.Joseph Fiorelino (2005-2006) Immunology IMM450Y.Jennifer Li (2006-2007) Immunology IMM450Y.Jerome Chan (2006-2007) Immunology IMM450Y.Dianne Wu (2007-2008) Immunology IMM450YDavid Shih (2007-2008) Immunology IMM450YLinda Zhao (2008-2009) Immunology IMM450YChristopher Yao (2009-2010) Immunology IMM450YNhat Tran (2009-2010) Immunology IMM450YYurij Baglaekno (2009-2010) Immunology 450Y

Eustache Paramithiotis (Ph.D. 1994) Supported by FCAR and NSERC.Tania Benatar (M.Sc., 1992) Supported by the Cancer Research Society. Winner

of the Wilfred Yaffe Award for top M.Sc. graduate (1992-3).Oswald Martinez (M.Sc., 1996)Sandra Demaries (M.Sc. 1997) Supported by a studentship from FCAR. Winner of

the Wilfred Yaffe Award for top M.Sc. graduate 1997-98)Mira Rao (M.Sc. 1999) Supported by a studentship from NSERC.Karen Jacobsen (post-doctoral fellow, 1994-98) Supported by MRC post-doctoral

fellowship.Camil Sayegh (Ph.D. 2000) Supported by an MRC studentship. Winner of the

Becton-Dickinson prize for best student poster presentation at the 13th

Canadian Society for Immunology Meeting.Kelly Pike (Ph.D. 2005) Supported by FRSQ.Parinaz Aliahmad (M.Sc. 2002) Supported by NSERC.Anton Neschadin (M.Sc. 2005)Sonja Kothlow (post-doctoral fellow, 2005) Supported by DAAD, Germany.Mani Larijani (Ph.D. 2003) co-supervisor with G. Wu.Emily Agard (Ph.D. 2005) co-supervisor with S. Lewis.Joseph Fiorelino (M.Sc. 2008)Dariush Tahamzadeh (Ph.D. in progress) Supported by CIHR.Alex Ling (Ph.D. in progress)

3. Graduate trainees, direct supervision

29

4. Graduate students indirect supervision

A. Duclos (Microbiology and Immunology, 1994-6)J. Lee (Biochemistry, 1995)J. Stewart (Microbiology and Immunology, 1993-96)L. Chow (Biochemistry, 1993)R. Srikumar (Microbiology and Immunology, 1992-96)B. Huang (Experimental Medicine, 1993-6)L. Petropolous (Microbiology and Immunology, 1995-98)X. Shan (Microbiology and Immunology, 1994-98)L. Haughn (Microbiology and Immunology, 1993-98)S. Klein (Experimental Medicine, 1994-97)G. Moeck (Microbiology and Immunology, 1992-96)D. Dahan (Microbiology and Immunology, 1993-97)J. Hu (Experimental Medicine, 1995-99)J. Albenese (Haematology, 1994-98)S. Lesage (Experimental Medicine, 1996-99)F. Phillappoussis (Microbiology and Immunology, 1998-2001)D. St. Dic (Microbiology and Immunology, 1997-2001)I. Doyle (Microbiology and Immunology, 1998-99)C. Moldovan (Microbiology and Immunology, 1998-2001)J.F. Poulin (Microbiology and Immunology, 1998-2002)K. Brassinger (Microbiology and Immunology, 1998-2001)L. Fawaz (Experimental Medicine, 2000-2001)M. Ciofani (Immunology, J.C. Zuniga-Pflucker, 2002-2007)R. de Pooter (Immunology, J.C. Zuniga-Pflucker, 2002-2008)J. Yuan (Immunology, C. Guidos, 2003-2008)C. Hammond (Medical Biophysics, D. Spaner, 2003-2008)C. Koscik (Immunology, G. Levy, 2003-2005) M.Sc 2005.D. Frieder (Immunology, A. Martin, 2003-2009)F. Guerra (Immunology, R. Rottapel, 2004-2009)E. Tang (Immunology, A. Martin, 2004-2007)D. McCarthy (Immunology, J. Gommerman, 2004-present)C. Loh (Immunology, J. Wither, 2004-present)I. Shalev (Immunology, G.Levy, 2005-2009)P. Urbanellis (Immunology, G. Levy, 2007-present)

CURRICULUM VITAE - McGill Universitypublications.mcgill.ca/.../04/CV-Dr.-Michael-Ratcliffe.pdf1 CURRICULUM VITAE Michael John Hayward Ratcliffe Department of Immunology, University

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