SIMPAR (Study In Multidisciplinary PAin Research)
SIMPAR (Study In Multidisciplinary PAin Research)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in chronic pain
Summary 1 Chronic pain and inflammation 2 Curcumin from Turmeric 3 Active principles of turmeric 4 Curcumin and chronic pain 5 Mechanism of action (antinflammatory) 6 Mechanism of action (anti oxidant) 7 Epigenetic activity of curcumin 8 Absortion of curcumine 9 Curcumin and liver 10 Bio-avaliability and solutions 11 Curcumin tolerability 12 Take home messages
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness express high grade of inflammation
Cardiovascular diseases
INFLAMMATION
Cancer
Auto-immune diseases
Neuro degenerative diseases
Type II diabetes
High grade or Low grade
For in
ternal u
se on
ly ndash Q1 2015
Symptoms of Inflammation
For in
ternal u
se on
ly ndash Q1 2015
Acute pathological pain bull Sharp fast pricking bull Occurs very rapidly bull Carried by large diameter myelinated Aδ fibers bull Usually alleviated with the help of professional
Chronic pathological pain bull Burning aching bull Gradually increases bull Carried by small diameter non-myelinated lsquoCrsquo fibers bull Persistent pain that lasts many months to years bull Little apparent cause amp not self limiting bull Pain often increases over time amp is aggravated by many factors
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in chronic pain
Summary 1 Chronic pain and inflammation 2 Curcumin from Turmeric 3 Active principles of turmeric 4 Curcumin and chronic pain 5 Mechanism of action (antinflammatory) 6 Mechanism of action (anti oxidant) 7 Epigenetic activity of curcumin 8 Absortion of curcumine 9 Curcumin and liver 10 Bio-avaliability and solutions 11 Curcumin tolerability 12 Take home messages
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness express high grade of inflammation
Cardiovascular diseases
INFLAMMATION
Cancer
Auto-immune diseases
Neuro degenerative diseases
Type II diabetes
High grade or Low grade
For in
ternal u
se on
ly ndash Q1 2015
Symptoms of Inflammation
For in
ternal u
se on
ly ndash Q1 2015
Acute pathological pain bull Sharp fast pricking bull Occurs very rapidly bull Carried by large diameter myelinated Aδ fibers bull Usually alleviated with the help of professional
Chronic pathological pain bull Burning aching bull Gradually increases bull Carried by small diameter non-myelinated lsquoCrsquo fibers bull Persistent pain that lasts many months to years bull Little apparent cause amp not self limiting bull Pain often increases over time amp is aggravated by many factors
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness express high grade of inflammation
Cardiovascular diseases
INFLAMMATION
Cancer
Auto-immune diseases
Neuro degenerative diseases
Type II diabetes
High grade or Low grade
For in
ternal u
se on
ly ndash Q1 2015
Symptoms of Inflammation
For in
ternal u
se on
ly ndash Q1 2015
Acute pathological pain bull Sharp fast pricking bull Occurs very rapidly bull Carried by large diameter myelinated Aδ fibers bull Usually alleviated with the help of professional
Chronic pathological pain bull Burning aching bull Gradually increases bull Carried by small diameter non-myelinated lsquoCrsquo fibers bull Persistent pain that lasts many months to years bull Little apparent cause amp not self limiting bull Pain often increases over time amp is aggravated by many factors
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Chronic and degenerative illness express high grade of inflammation
Cardiovascular diseases
INFLAMMATION
Cancer
Auto-immune diseases
Neuro degenerative diseases
Type II diabetes
High grade or Low grade
For in
ternal u
se on
ly ndash Q1 2015
Symptoms of Inflammation
For in
ternal u
se on
ly ndash Q1 2015
Acute pathological pain bull Sharp fast pricking bull Occurs very rapidly bull Carried by large diameter myelinated Aδ fibers bull Usually alleviated with the help of professional
Chronic pathological pain bull Burning aching bull Gradually increases bull Carried by small diameter non-myelinated lsquoCrsquo fibers bull Persistent pain that lasts many months to years bull Little apparent cause amp not self limiting bull Pain often increases over time amp is aggravated by many factors
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Symptoms of Inflammation
For in
ternal u
se on
ly ndash Q1 2015
Acute pathological pain bull Sharp fast pricking bull Occurs very rapidly bull Carried by large diameter myelinated Aδ fibers bull Usually alleviated with the help of professional
Chronic pathological pain bull Burning aching bull Gradually increases bull Carried by small diameter non-myelinated lsquoCrsquo fibers bull Persistent pain that lasts many months to years bull Little apparent cause amp not self limiting bull Pain often increases over time amp is aggravated by many factors
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Acute pathological pain bull Sharp fast pricking bull Occurs very rapidly bull Carried by large diameter myelinated Aδ fibers bull Usually alleviated with the help of professional
Chronic pathological pain bull Burning aching bull Gradually increases bull Carried by small diameter non-myelinated lsquoCrsquo fibers bull Persistent pain that lasts many months to years bull Little apparent cause amp not self limiting bull Pain often increases over time amp is aggravated by many factors
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Drugs used for pain management
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Most sold SOP and OTC in Italy 2011
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Precautions in patients gt70 years
GI side effects
Risks of gastic ulcers
Attention in patients treated with anti-coagulatns
Risk of kidney impairment
Side effects and risks of NSAIDrsquos
ldquoGli utilizzatori di FANS hanno ulcere sintomatiche complicate 3-4 volte piugrave frequentemente rispetto ai non utilizzatorirdquo 2
ldquoDisturbi gastrici e intestinali compaiono nel 10 - 30 degli utilizzatori di FANSrdquo 1
1-2Nederlandse Huisartsen Genootschap ndash pijnbestrijding farmacotherapeutische richtlijn
3Cannon 2006 Chan 2006 Helin-Salmivaara 2006 Kearney 2006 McGettigan 2006
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Curcuma longa
Boswellia serrata
bull Omega 3 (EPA and DHA)
Zingiber officinale
Natural substances with anti-inflammatory activity
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
bull Rhizomatous herbaceous perennial plant
bull Family Zingiberaceae bull Lives well with mild temperatures
(20deg-30deg C and high rains bull Reach 1 m in hight bull Nice hermafrodite flowers in 20cm
inflorescence (August) bull Active sustances are concentrated
in rhizomes
Turmeric the plant (Curcuma longa)
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Curcumin THE active principle of turmeric
(17-bis (4-hydroxy-3-methoxy-phenyl) hepta-16-diene-35-dione)
Turmeric powder contains 3-7 of Curcuminoids essential oils and fibers
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
8770 studies shows pleiotroitropic effects of curcumin (Pubmed)
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
23
24
25
26
27
28
29
0
5
10
15
20
0
5
10
15
20
ldquoRandomized single blind study on efficacy and tolerability of curcumin in patients with reumatoid poliarthritis ldquo Chandran et al A Randomized Pilot Study to Assess the Efficacy and Safety of Curcumin in Patients with Active Rheumatoid Arthritis Phytother Res 2012261719ndash1725
deg n = 45 curcumin n =15 diclofenac n = 15 curcumin and diclofenac n = 15 deg 8 weeks 1000 mg curcumin (BCM-95 ) e 100 mg diclofenac
Curcumin vs NSAIDrsquo (diclofenac )
Tass
o d
i sed
imen
tazi
on
e er
itro
cita
ria
(ESR
)
- 11 - 8
Significative reduction of Disease Activity Score (DAS)
Significative reduction of Joints pain
- 83 - 69
- 97 - 89
Significative reduction of Joints swelling
In
cid
enza
In
cid
enza
Curcumin extract Diclofenac 1000 mg 100 mg
p lt 005 p lt 005 p lt 005
Curcumin extract Diclofenac 1000 mg 100 mg
Curcumin extract Diclofenac 1000 mg 100 mg
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
280
290
300
310
320
330
340
350
360
Ibuprofen Curcumina
deg n = 367 curcumin extract n = 182 ibuprofen n = 185 deg After 4 weeks 1500 mg curcumin (2 capsuls 3xdie) e 1200 mg ibuprofen (2 capsuls 3xdie) deg Simple turmeric extract
Mobility Distance covered in 6 minutes
(Time 0 and after 4 weeks)
p = 0010
Met
ers
wal
ked
in 6
min
ute
s
Chronic Pain (Time 0 and after 4
weeks)
0
1
2
3
4
5
6
Ibuprofen Curcuminap = 0018
In
cid
ence
1200 mg 1500 mg
1200 mg 1500 mg )
- 41 + 14 + 11
Curcumin vs NSAIDrsquo (ibuprofen)
ldquoEfficacy and safety of curcumin vs Ibuprofen in patients with knee arthrosis a multicentric studyldquo
Kuptniratsaikul V et al Efficacy and safety of Curcuma domestica extracts compared with ibuprofen in patients with knee osteoarthritis a multicenter study Clin Interv Aging 2014 Mar 209451-8
- 39
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Combination of Curcumin an ginger is effective as well as NSAIDrsquoS (indometacine)
Objective measure effective of a blend of curcumin and ginger on pain in patients with rheumatoid arthritis 3 groups NSAIDrsquoS (Indometacine) vs 200 mg (CG) vs 400 mg (CG) Results
Arthritic score 14d
Arthritic score 28d
Nsaidrsquos (Indometacine)
396 50
200 mg 417 583
400 mg 521 786
0
4
8
12
16
7 giorni 14 giorni 28 giorni
Controllo (dopo trattamento) Indometacina
200 mg curcumina +zenzero 400 mg curcumina+zenzero
- 786 - 52 A
rth
riti
c sc
ore
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
deg n = 15 curcumin 400 mg nimesulide 100mg achetominophen 1g Cicle with 3 subtances with a wash out of 48 hour
Curcumin vs Nimesulide and Acetoaminophene in acute pain
Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Merivareg) nimesulide and acetaminophen Di Pierro F1 Rapacioli G Di Maio EA Appendino G Franceschi F Togni S
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Curcumin studies in neuropatic pain in animal model
Zhao X Xu Y Zhao Q Chen CR Liu AM et al (2012) Curcumin exerts antinociceptive effects in a mouse model of neuropathic pain descending monoamine system and opioid receptors are differentially involved Neuropharmacology 62 843ndash854 14 Ji FT Liang JJ Liu L Cao MH Li F (2013) Curcumin exerts antinociceptive effects by inhibiting the activation of astrocytes in spinal dorsal horn and the intracellular extracellular signal-regulated kinase signaling pathway in rat model of chronic constriction injury Chin Med J (Engl) 126 1125ndash1131
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Curcumin in cancer patients
Objective Efficacy of curcumin in cancer patient ongoing chemio or radiotherapy measured with Karnofsky Scale as developed by Karnofsky Abelmann Craver and Burchenal - 60 days with curcumin or placebo Results
Curcumin 100 mg
A Controlled Study of a Lecithinized Delivery System of Curcumin to alleviate the Adverse Effects of Cancer Treatment
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
Pubmed 18112015 2367 publications
Curcumin anti-oxidant power is 10 time higher than Vit E - Direct activity on ROS - Induction of NRF2 - Induce mitocondrial biogenesis
Curcumin is a powerful anti-oxidant
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Inflammatory targets modulated by curcumin
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
Epigenetic activity of Curcumin
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Epigenetic activity of curcumin
Molecular targets Activity Epigenetic and anti-cancer activity under evaluation
DNA methylation HAT activities Histone acetylation (Acetil transferasehistone deacetilase HDAC isoenzymes (HDAC 13458)
miRNArsquos
miRNArsquos target genes
Decrease Decrease Rebalance Reduce expression Modulation Regulation
Anti-oxidant anti-angiogenic antiproliferative pro-apoptotic
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Bioavailability of Curcumin is very poor
Poor absorption Max conc 180 minutes Liver cleverage Cit P450
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
Curcuma polvere
Curcumina principio attivo (1)
Fitosoma (1)
Oli (1)
Ciclodestrina (1)
Forma micellare o Solubilizzata (2)
X 95 ndash 511
X 577
X 335
X 1350
X 73
Source bull (1) Jaumlger et al Nutrition Journal 2014 1311 comparative absorption of curcumin formulations bull (2) Schiborr et al Mol Nutr Food Res 2014 58 516-527
X 1
Biodisponibilitagrave vs Curcuma
Bioavailability of IMPROVED Curcumins
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
bull Curcuma longa is GRASS in the USA
Turmeric tolerability
bull Curcumin is not carcinogenic bull No concerns of genotoxicity bull ADI of 3 mgkg bwday based on the NOAEL of 250-320
mgkg bwday bull Intake of curcumin from the normal diet amounts to less than
7 of the ADI of 3 mgkg bwday bull Possible weak interference with anti-coagulant that normally
do not require dosage adjustments
ADI = Accettable daily Intake
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Synergistic activity with
bull Nsaidrsquos
bull Steroids
bull Omega 3
bull Boswellia
Inflammation anc chronic pain
bull Omega3
bull Vit B complex
bull Ginko biloba
bull Anti-oxidants
Peripheral
Neuropatic pain and neurodegeneration
bull Glutammine
bull Probiotics
bull Prebiotics
bull Anti-oxidants
Gastrointestinal health
bull Glucosammine
bull Chondroitine
bull MSM
bull Boswellia
Joints Health
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
Ongoing clinical trials with curcumin
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
For in
ternal u
se on
ly ndash Q1 2015
DAI Medicina Interna Medicina Specialistica UO Medicina Interna Universitaria ldquoCesare Frugonirdquo
Centro di Assistenza e Ricerca Sovraziendale per le Malattie Rare
Universitagrave Degli Studi ldquoALDO MOROrdquo di Bari
Direttore Prof Carlo Sabbagrave
REGIONE PUGLIA
AZIENDA OSPEDALIERA OSPEDALE POLICLINICO CONSORZIALE
Piazza Giulio Cesare 11 ndash 70124 BARI
Testing 3 ldquolevelsrdquo of curcumin activity
ANALYSYS OF GENIC EXPRESSION OF MONONUCLEATE BLOOD CELLS OF PATIENTS WITH METABOLIC SYNDROME AND OSTEOARTHROSIS POTENZIAL APPLICATIONS IN DIAGNOSIS MONITORING AND TREATMENT AFTER 3 MONTHS OF SUPPLEMENTATION WITH CURCUDYN (MSCPBMCCURCUMIN15)
First Level GENE EXPRESSION
bull Quantitative analysis of (mRNA) PCR Quantitative Real Time (RT-qPCR) RT-qPCR Primer
bull SNPrsquos Genotyping on DNA
Second Level INFLAMMATIONrsquos MARKERS
bull C Reactive Protein bull TNF alfa bull IL 6 IL 10
Third Level SYMPTOMS AND LIFE QUALITY
bull Chronic pain (Forms) bull Mobility bull Quality of Life (Form ILMA)
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
Take home messages
Curcumin is effective on chronic pain and inflammation as NSAIDrsquos laquoEnhanced raquo curcumins have better bioavailability Curcumin is safe can be used with all patients
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS
Contatti msal37gmailcom +39 3663461468
Social httpswwwlinkedincominmauriziosalamone
httpmauriziosalamoneblogspotit
httpwwwslidesharenetMaurizio37
httpswwwfacebookcommauriziosalamone
Maurizio Salamone biologist Science Manager Metagenics Italia srl DISCLAIMER MSALAMONE IS AN EMPLOYEE OF METAGENICS A GLOBAL COMPANY THAT MARKETS NUTRACEUTICALS