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NETZWERK ALGEN 2018, TULLN 1 Jiří MASOJÍDEK Institute of Microbiology of the CAS, Centre ALGATECH, T řeboň University of South Bohemia, Faculty of Science, České Budějovice Cultivation of Microalgae Photobioreactors and Contamination
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Cultivation of Microalgae Photobioreactors and Contamination

Feb 19, 2022

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Page 1: Cultivation of Microalgae Photobioreactors and Contamination

NETZWERK ALGEN 2018, TULLN 1

Jiří MASOJÍDEKInstitute of Microbiology of the CAS, Centre ALGATECH, TřeboňUniversity of South Bohemia, Faculty of Science, České Budějovice

Cultivation of Microalgae

Photobioreactors and Contamination

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Outline

o ALGATECH Centre - Microalgae Biotechnology in Třeboň

o Cultivation systems

o Contamination - Anti-contamination strategies

PROJECTS

o EU H2020 SABANA project (2017-2020)o Interreg project ALGENETICS

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About ALGATECH Centre – since 2011

Topics in Microalgae ResearchPhysiology / Photosynthesis / Molecular Biology / Biophysics /

Biochemistry / Cell Biology / Biotechnology

Laboratories:Photosynthesis / Cell Cycles / Photosynthetic Bacteria

Algal Biotechnology

NETZWERK ALGEN 2018, TULLN

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Cultivation Systems

TWO TYPES

Open systemsPonds, raceways

cascades

Closed or semi-closedsystems = PBRs

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Open Production Systems for Microalgae Cultivation

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Outdoor PBRs for Microalgae Cultivation

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Greenhouse PBRs or Microalgae Cultivation

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Maintenance, cleaning, contamination

OPEN PONDS RACEWAYSCASCADES

CLOSED OR SEMI-CLOSED

PBRs

o Cheap construction and

maintenance

o Cleaning easy

o Low control of culture conditions

o Evaporation – cooling !!!

o Contamination !!!

o Higher costs for construction and

maintenance

o Cleaning !!!

o Good control of culture conditions

o Cooling !!!

o Low Contamination

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Strain selection

OPEN PONDS RACEWAYSCASCADES

CLOSED OR SEMI-CLOSED

SYSTEMS=PBRs

o Species specific

o Fast-growing, robust strains –Chlorella, Scedesmus, etc. (Chlorophyta)

o Specific conditions - Spirulina (bicarbonate , high pH), Dunaliella(high salinity), marine Nannochloropsis

o Spectrum of specieso Production strains –

Haematococcus, Chlorellamixotrophic

o Strains for high-requirementso Aquaculture strains – Isochrysis,

Tetraselmis, Phaeodactylum, Skeletoma

o Cultivation of GMO

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Contamination / Infection in Microalgae Cultures

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Anti-contamination Strategies

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Open Outdoor Systems – Contamination

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Contamination in PBRs - Synechocystis vs. Poterioochromonas

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Case Studies – Microalgae in Agriculture

Sustainable Integrated Algae Biorefineryfor the Production of BioactiveCompounds for Agriculture aNdAguacultureEU H2020 Research and InnovationProgramme (2016-2020)Coordinator: University of Almería,Spain (prof. Gabriel Acién)

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Block Diagram of SABANA Project

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Task of ALGATECH team

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Laboratory cultivation of Synechocystis PCC6803

10-L PBR

25-L fully controlledFlat Panel PBR 100-L PBR

with internal illumination

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ecoduna

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o Karolína Ranglová, Soňa Pekařová, Gergély Lakatos, Tomáš Grivalský, Richard Lhotský, Centre Algatech Institute of Microbiology, Academy of Sciences, Třeboň

o Giuseppe Torzillo, Margarita Da Silva, Istituto per lo Studio degli Ecosistemi CNR, Sesto Fiorentino, Italy

o Gabriel Acién, Cintia Gómez, Francesca Suarez (joint experiments),University of Alméria, Spain

o Felix Figueroa, University of Málaga, Spaino Vince Ördög, Széchenyi István University in Mosonmagyaróvár, Hungary

(strains)o Juliane Richter, Richard Gundolf, FH OÖ Forschungs & Entwicklungs

GmbH in Wels

Fundingo EU H2020 project SABANAo Interreg At-Czo Algatech Plus - Ministry of Education, Youth and Sports, CR

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8TH EUROPEAN ALGAE SUMMIT, VIENNA 22

Thank you for attention

[email protected]

Questions, remarks?

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From Collection to Small Scale Culture

1st – collection of strains on agar

2nd step – 400 mL

5th step – 2.000 L