X. Revelo*, A. Kenny, N. Barkley, and M. Waldron Division of Animal Sciences University of Missouri, Columbia Impaired capacity of neutrophils to produce reactive oxygen species, release extracellular traps and express genes encoding for cytokines may contribute to altered immune function in periparturient dairy cows
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X. Revelo*, A. Kenny, N. Barkley, and M. WaldronDivision of Animal Sciences
University of Missouri, Columbia
Impaired capacity of neutrophils to produce reactive oxygen species, release extracellular
traps and express genes encoding for cytokines may contribute to altered immune
function in periparturient dairy cows
Neutrophils are key immune cells
Killing
Chemotaxis
Neutrophil killing mechanisms
ExtracellularIntracellular
Reactive oxygen species (ROS)
Neutrophil extracellular traps (NETs)
Kennedy 2009 Brinkmann 2004
Objective
Investigate changes in gene expression associated with neutrophil dysfunction during the periparturient period in dairy cows- Neutrophil function:- Reactive oxygen species (ROS) production
- Extracellular trap formation (NET)
- Chemotaxis towards interleukin-8
- Phagocytosis of E. coli
-Gene expression:-Tumor necrosis factor-α mRNA
-Interleukin-8 mRNA
-Cytochrome-b mRNA
-Bactericidal/permeability increasing protein (BPI) mRNA
-Transcriptome (mRNA) shotgun sequencing
Research Methods
• Collection of blood Neutrophil function (n = 35)Late pregnancy: -58 ± 1.7 dPre-partum: -7 ± 0 dPostpartum: 6 d