Creation of conditions for production of strawberry planting material using tissue culture Doc.dr. Semina Hadžiabulić A G R O M E D I T E R A N S K I F A K U L T E T - M O S T A R UNIVERZITET ”DŽEMAL BIJEDIĆ”- MOSTAR AGROMEDITERANSKI FAKULTET Adresa:USRC Midhad Hujdur-Hujka, 88 004 Mostar Studentska služba: Tel/Fax: 036 571 388 E-mail: [email protected]
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Creation of conditions forproduction of strawberry planting
material using tissue culture
Doc.dr. Semina Hadžiabulić
AG
RO
MED
IT
ERANSKI FAKULTET-MO
STAR
UNIVERZITET ”DŽEMAL BIJEDIĆ”- MOSTARAGROMEDITERANSKI FAKULTET
Adresa:USRC Midhad Hujdur-Hujka, 88 004 MostarStudentska služba: Tel/Fax: 036 571 388
E-mail: [email protected]
• Though we had a lot of theoretical knowledge about production of plants using of tissue culture method, we started to deal with practical problems when we began the implementation of this project.
The first and the most important condition was to provide sterile conditions
Trebamo obezbjediti1. sterile conditions for cultivation mother
plants (glasshouse)2. sterile conditions prostorija laboratorije • Laminar (explant handling)• Autoclave
Planning of the laboratory space
Laboratory equipment:• The space provided for making of
nutrient medium• The space for sterile handling with
plant meterial• The storage space for cultures storing
1. Sterile conditions for cultivation
Desinfection of plant material
• I case:• No need for desinfections
• II case :• 70% ethanol (30 sec.);• Soak in distilled water (1 min.)
• III case:• 70 % ethanol (30 sec.)• 0,5 % NaHCl+ 1-2 drops Citowett/ 0,5L
NaHCl (10 min.)• Multiple washing in distilled water (3,
10, 20 min.)
Equipment for sterile handling with plant material
Laminar
Sterilization of nutrient medium
Clime chamber
Related Documents
