CRC FISH data Summary teleconference 7/11/07 TJ Kipps (UCSD) Laura Rassenti (UCSD): data analysis Andrew Greaves (UCSD): data retrieval Data input: Dan Van Dyke (Mayo) Nyla Heerema (OSU) Paola Dal Cin (DF) Marie Dell’Aquila (UCSD) Prasad Koduru (LIJ) Bill Wierda (MDA) Donna Neuberg (DF-Stats) David Zahrieh (DF-Stats)
CRC FISH data Summary teleconference 7/11/07
Jan 30, 2016
CRC FISH data Summary teleconference 7/11/07. TJ Kipps (UCSD) Laura Rassenti (UCSD): data analysis Andrew Greaves (UCSD): data retrieval Data input: Dan Van Dyke (Mayo) Nyla Heerema (OSU) Paola Dal Cin (DF) Marie Dell’Aquila (UCSD) Prasad Koduru (LIJ) Bill Wierda (MDA) - PowerPoint PPT Presentation
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CRC FISH data Summaryteleconference 7/11/07
TJ Kipps (UCSD)
Laura Rassenti (UCSD): data analysis
Andrew Greaves (UCSD): data retrieval
Data input:
Dan Van Dyke (Mayo)
Nyla Heerema (OSU)
Paola Dal Cin (DF)
Marie Dell’Aquila (UCSD)
Prasad Koduru (LIJ)
Bill Wierda (MDA)
Donna Neuberg (DF-Stats)
David Zahrieh (DF-Stats)
CRC Tissue Core Cytogenetics datacompleteness report (7/1/07)
Site (# CLL pts) Cyto Data (% complete)
UCSD (N=660) 41%
MDA (N=1269) 74%
OSU (N=585) 67%
DF (N=468) 69%
LIJ (N=598) 12%
Mayo (N=371) 51%
CRC FISH Data Summary(as of 7/1/07)
Normal cut off (Mayo)
Chr11q del 5.0%
Chr12+ 1.5%
Chr13q del 7.0%
Chr17p del 8.5% & 20%
Correlations with %ZAP & %VH
% cells with Chr 11 abnormal vs % ZAP-70
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100
% ZAP-70
% cells with Chr 11 abnormal
% cells with Chr 11 abnormal vs % VH
0
10
20
30
40
50
60
70
80
90
100
60 62 64 66 68 70 72 74 76 78 80 82 84 86 88 90 92 94 96 98 100
% VH homology
% cells with Chr 11 abnormal
0
10
20
30
40
50
60
70
80
90
100
N=477Mean=6.3SD=20.5
N=381Mean=10.8SD=25.6
ZAP % Chr11 abnormal
p=0.0040*
0
10
20
30
40
50
60
70
80
90
100
Mean=1.8SD=11.1N=405
Mean=14.1SD=28.6N=453
VH % Chr11 abnormal
p<0.0001*
Chr 11 % abnormal cells vs ZAP+/- & VH+/-
0
10
20
30
40
50
60
70
80
90
100
N=738Mean=25.0SD=26.8
N=120Mean=28.6SD=21.8
% ZAP Chr11 abnormal(5% cut off)
p=0.1618 ns
60
70
80
90
100
Mean=96.2SD=4.3N=738
Mean=99.2SD=1.7N=120
% VH Chr11 abnormal(5% cut off)
p<0.0001*
Chr 11 % abnormal cells vs ZAP & VH(5% cut off)
% cells with Chr 12 abnormal vs % ZAP-70
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100
% ZAP-70
% cells with Chr 12 abnormal
% cells with Chr 12 abnormal vs % VH
0
10
20
30
40
50
60
70
80
90
100
60 62 64 66 68 70 72 74 76 78 80 82 84 86 88 90 92 94 96 98 100
% VH homology
% cells with Chr 12 abnormal
Chr 12 % abnormal cells vs ZAP+/- & VH+/-
0
10
20
30
40
50
60
70
80
90
100
N=512Mean=3.7SD=14.0
N=394Mean=11.0SD=24.0
ZAP % Chr12 abnormal
p<0.0001*
0
10
20
30
40
50
60
70
80
90
100
VH % Chr12 abnormal
Mean=3.6SD=14.2N=433
Mean=9.8SD=23.5N=473
p<0.0001*
Chr 12 % abnormal cells vs ZAP & VH(1.5% cut off)
0
10
20
30
40
50
60
70
80
90
100
N=783Mean=23.1SD=24.7
N=123Mean=36.1SD=28.7
% ZAP Chr12 abnormal(1.5% cut off)
p<0.0001*
60
70
80
90
100
% VH Chr12 abnormal(1.5% cut off)
Mean=96.4SD=4.2N=783
Mean=97.6SD=3.7N=123
p=0.0024*
% cells with Chr 13 abnormal vs ZAP-70
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100
% ZAP
% cells with Chr 13 abnormal
Percent_Abnormal_13
% cells with Chr 13 abnormal vs % VH
0
10
20
30
40
50
60
70
80
90
100
58 62 66 70 74 78 82 86 90 94 98
% VH
% cells with Chr 13 abnormal
Chr 13 % abnormal cells vs ZAP+/- & VH+/-
0
10
20
30
40
50
60
70
80
90
100
N=516Mean=34.23SD=34.0
N=398Mean=26.6SD=34.9
ZAP % Chr13 abnormal
p=0.001*
0
10
20
30
40
50
60
70
80
90
100
Mean=34.7SD=33.6N=437
Mean=27.4SD=35.4N=477
VH % Chr13 abnormal
p=0.0014*
Chr 13 % abnormal cells vs ZAP & VH(7.0% cut off)
0
10
20
30
40
50
60
70
80
90
100
N=417Mean=29.2SD=26.8
N=497Mean=21.6SD=24.4
% ZAP Chr13 abnormal(7% cut off)
p<0.0001*
60
70
80
90
100
Mean=97.2SD=3.7N=417
Mean=96.0SD=4.4N=496
% VH Chr13 abnormal(7% cut off)
p<0.0001*
% cells with Chr17 abnormal vs ZAP-70
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100
% ZAP-70
% cells with Chr17 abnormal
% Cells with Chr17abnormal vs % VH
0
10
20
30
40
50
60
70
80
90
100
50 54 58 62 66 70 74 78 82 86 90 94 98
% VH homology
% cells with Chr17 abnormal
Chr 17 % abnormal cells vs ZAP+/- & VH+/-
0
10
20
30
40
50
60
70
80
90
100
ZAP % cells Chr17 abnormal
Mean=3.3SD=13.2N=502
Mean=7.2SD=21N=389
p<0.0001*
0102030405060708090
100
%
VH % cells with Chr17 abnormal
p<0.0001*
Mean=1.6SD=8.6N=427
Mean=8.1SD=22N=468
Chr 17 % abnormal cells vs ZAP & VH(8.5% cut off)
0102030405060708090
100
%
% ZAP Chr17 abnormal(8.5% cut off)
p=0.0035*
Mean=24.2SD=25.6N=796
Mean=32.3SD=27.5N=95
60
70
80
90
100
% VH Chr17 abnormal(8.5% cut off)
Mean=96.4SD=4.2N=796
Mean=98.2SD=3.0N=95
p<0.0001
Chr 17 % abnormal cells vs ZAP & VH(20% cut off)
0
10
20
30
40
50
60
70
80
90
100
% ZAP Chr17 abnormal(20% cut off)
Mean=24.1SD=25.4N=830
Mean=38.1SD=27.5N=61
p<0.0001*
60
70
80
90
100
% VH Chr17 abnormal(20% cut off)
Mean=96.4SD=4.2N=830
Mean=98.9SD=2.6N=61
p<0.0001*
%Cells with abnormal Chr17
0 5 10 15 20 25 30 35 400
10
20
30
40
50
60
70
80
90
100
0-20 % abn17(3.7 yrs)
20-100%abn17(2.7 yrs)
Time(yrs)
0 2 4 6 8 10 12 14 16 18 200
10
20
30
40
50
60
70
80
90
100 0-10 % abn17(3.7 yrs)
10-20%abn17
20-30%abn17(4.0 yrs)
30-40%abn17(6.0 yrs)
40-70%abn17(2.7 yrs)
70-100%abn17(1.6 yrs)
Time from Dx to Tx% cells with abnoram Chr 17
(4.9 yrs)
Time(yrs)
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