Cost action FA1003 - GRAPENET East-West …digital.csic.es/bitstream/10261/116981/1/Poster_ocete_campos.pdf · East-West Collaboration for Grapevine Diversity Exploration and Mobilization

Cost action FA1003 - GRAPENET

East-West Collaboration for Grapevine Diversity Exploration and

Mobilization of Adaptive Traits for Breeding

FULL PROGRAM & ABSTRACT BOOK

Final Conference

PROGRESS IN VITIS VINIFERA

DIVERSITY EVALUATION AND USE 7-8 October 2014

Final Management committee

9 October 2014

INIAV - Instituto Nacional de Investigação Agrária e Veterinária Av. da República, Quinta do Marquês, Oeiras (Lisbon - Portugal)

Edited by Gabriella De Lorenzis, Laura Rustioni and Osvaldo Failla

Ecological characterization of wild grapevine habitats focused on arbuscular mycorrhizal symbiosis

Introduction: Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi is the dioecious relative of cultivated

vines. Due to the absence of human selection, this subespecies can be an important phytogenetic

resource for the viticulture. Arbuscular mycorrhizal (AM) colonise the roots of the above 90% of

land plants, including grapevines (Fig. 3), that are even fully dependent on AM. This symbiosis

increases plant growth and abiotic stresses tolerance. The knowledge about susceptibility and

effectiveness of wild grapevine to AM-association is not documented. The aim of the present work

is to carry out its preliminary study. Material and Methods: Samples of soil (1 kg) were collected in 18 populations (Spain and

France). Rhizosphere soil (50 g) was used for isolation and enrichment of AM fungi associated to

the target plants. AM identification is based on ontogenic, morphology and formation of the spores

and the structure of their wall. Results: The main native mite species on vines are the erineum strain of Colomerus vitis

(Pagenstecher) and Calepitrimerus vitis (Nalepa) (Acari, Eriophyidae). Symptoms caused by downy

and powdery mildews are very frequent. AM community composition presents a marked distribution

influenced by the pH of the soils. Glomus badium is the most generalist species, isolated from 8

locations, Glomus rubiforme in 7, Claroideoglomus claroideum in 5 and Funneliformis mosseae in 3

alkaline soils. The highest diversity was found in Peñaladros (Fig. 1) , with16 species and the lowest

diversity in Agrio river and El Bosque with only one species observed. Population name

and Province

Intervals of longitude and latitude

pH Main Botanical supporters AM species (in black corresponding photograph number on the right)

El Chorreadero (Cádiz)

36º49’26’’N – 36º49’17’’N 5º29’55’’W – 5º29’35’’W

8.2 Fraxinus angustifolia, Nerium oleander,

Salix sp.

Ambispora sp (5). Claroideoglomus claroideum (6) Diversispora sp (10), Glomus badium (24)

Glomus rubiforme (29)Glomus sp. (1,2,34) Pantano de los

Hurones (Cádiz)

36º42’43’’N – 36º42’28’’ N 5º33’47’’W – 5º33’51’’ W

7.9 Fraxinus angustifolia, Nerium oleander,

Populus nigra

Non described species (12) Funneliformis mosseae (14), Funneliformis sp. 2 (16)

Glomus intraradices (24) Glomus rubiforme (29) Glomus sp. (5,6)

El Bosque (Cádiz)

36º44’38’’N – 36º44’36’’ N 5º30’13’’W – 5º30’14’’W

7.9 Alnus glutinosa, Fraxinus angustifolia, Nerium oleander

Glomus sp?. 8 irregular type

Saratxo (Álava)

43º 1' 30,4’’N 3º 0´ 33,2’’W

7.9 Corylus avellana¸ Populus tremula,

Salix triandra

Claroideoglomus claroideum (6), Claroideoglomus etunicatum (7), Glomus badium (24), Glomus

macrocarpum (26) Glomus magnicaule, type (27) Glomus sp. 12

Septoglomus constrictum (32) Peña Angulo

(Burgos)

43º2'28,4’’N 3º11'26,1’’ W

9.4 Corylus avellana¸Populus tremula, Crataegus monogyna

Glomus badium (24) Etunicatum type

Cadagua river (Vizcaya)

43º 14´25’’N 2º59´50’’W

8.7 Acer campestre,Corylus avellana, Tilia cordata

Acaulospora sp. 2 (2), Ambispora sp. Glomus sp. 5 Claroideum type constrictum 1 type

La Concha (Vizcaya)

43º 13´30’’N 3º21´39’’W

9.1 Acer campestre, Acer monspessulanus, Corylus avellana

Glomus microagregatum (28), Glomus sp. 7 Claroideum type

Casalarreina (La Rioja)

42º 32’ 28.2’’N 2º 54’ 52.5’’W

7.6 Corylus avellana¸ Crataegus monogyna, Populus nigra

Claroideoglomus etunicatum (7) Glomus sp. 13, ocultum type

Peñaladros (Burgos)

43º3'45,1’’N 3º8'59,5’’W

8.2 Corylus avellana, Crataegus monogyna,

Tilia cordata

Acaulospora sp. 2 (2), Claroideoglomus claroideum (6) Claroideoglomus sp.(1,2) (8) (9)

Glomoide (1,2) (19) (20), Glomus rubiforme (29) Glomus sp. (9,13,15,16,18), Paraglomus ocultum (33)

Scutellospora sp .2, intraradices type

Saint Jean de Luz (Lapurdi)

43º 24´34''N 1º37´23’’W

7.6 Corylus avellana, Fraxinus latifolia,

Glomoide (4,5) (22) (23) Glomus intraradices (25)

Sobrón (Álava)

42º45´36’’N 3º05´38’’W

8.0 Arbutus unedo, Corylus avellana,

Quercus ilex,

Entrophospora infrecuents (11), Funneliformis sp. 1 (15), Glomus badium (24)

Glomus magnicaule type (27), Septoglomus sp

Valle de Mena (Burgos)

43º9'33,3’’N 3º13'38,7’’W

7.6 Corylus avellana¸ Crataegus monogyna,

Populus nigra

Claroideoglomus claroideum (6) Glomoide 3 (21)

Glomus badium (24) La Minilla (Sevilla)

37º39’34’’ N – 37º40’7’’N 6º9’25’’ W - 6º10’9’’W

7.3 Alnus glutinosa, Fraxinus angustifolia,

Nerium oleander

Acaulospora sp. 4 (4) Funneliformis sp. 3 (17) Glomus rubiforme (29)

Río Agrio (Sevilla)

37º 30’ 47.5’’N 6º 13’ 24.6’’W

7.2 Fraxinus angustifolia, Ficus carica, Populus nigra

Glomus sp. 11

Casas del Monte M1 (Cáceres)

40º 13’15.58’’N 5º 58’42.6’’W

6.2 Fraxinus angustifolia, Quercus suber, Populus nigra,

Claroideum type Intraradices type

Casas del Monte M2 (Cáceres)

40º13’15.58’’N 5º58’42.6’’W

5.6 Fraxinus angustifolia, Populus nigra

Acaulospora sp.1 (1), Gigaspora margarita (18) Glomus rubiforme (29), Scutellospora sp.1

Intraradices type, Tricispora nevadensis (31) Zarza-Granadilla

(Cáceres)

40º12’31.12’’N 6º0’10.53’’W

5.5 Fraxinus angustifolia, Ficus carica, Populus nigra

Funneliformis coronatus (13), Funneliformis mossae (14) Glomus rubiforme (29)

El Acebrón (Huelva)

37º8’32’’N 6º32’46’’W

5.4 Ficus carica, Pistacia lentiscus, Quercus suber

Glomus sp.10 Tricispora nevadensis (31)

Table 1 Locations, main ecological characteristics and AM species determined of the different places

Ocete, R.1), Armendáriz, I.2), Cantos, M.3), Álvarez, D4) and Azcón, R4) 1) Laboratorio de Entomología Aplicada. Facultad de Biología. Universidad de Sevilla. Spain.2)Universidad Fuerzas Armadas-

ESPE. Sangolquí, Ecuador. 3)Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS). CSIC. Spain. 4)Estación

Experimental del Zaidín (EEZ). Granada. CSIC. Spain.

Figure 1. Population of

PEÑALADROS

Figure 2. Typical structures of

Mycorrhizae on wild vine roots

obtained in field.

31 32 33

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POSTERS

SESSION 1 - Germplasm identification and characterization

1. Grapevine germplasm from Central Asia: evaluation of genetic diversity and relationships using 22 SSR markers, by Annarita MARRANO, Lukasz GRZESKOWIAK, Paula MORENO-SANZ, Silvia LORENZI, Maria Lucia PRAZZOLI, Arkadiy ARZUMANOV, Mahfurat AMANOVA, Osvaldo FAILLA, David MAGHRADZE, Maria Stella GRANDO

2. Genetic investigations on a large Italian grape collection by using SNP markers, by Giorgio TUMINO, Caterina MORCIA, Stefano RAIMONDI, Massimo GARDIMAN, Anna SCHNEIDER, Valeria TERZI

3. Ampelographic characteristics of Azerbaijani local grape varieties, by Vugar SALIMOV, Mirza MUSAYEV, Rauf ASADULLAYEV

4. Study of some old Albanian grapevine cultivars by ampelography and microsatellite markers, by Frida CARKA, Erika MAUL, Rajmonda SEVO

5. Cultivars identity and origin analyzed within extended Western Balkan grapevine germplasm, by Lidija TOMIC, Natasa STAJNER, Jose VOUILLAMOZ, Branka JAVORNIK

6. Grapevine collection of the Zurich University of Applied Sciences, by Johannes FAHRENTRAPP, Peter SCHUMACHER

7. The Italian Vitis Database: a multidisciplinary approach for a better knowledge and valorization of Italian grape

genotypes, by Serena IMAZIO, Maria Gabriella BARBAGALLO, Cristina BIGNAMI, Manna CRESPAN, Laura DE PALMA, Claudio D’ONOFRIO, Massimo MUGANU, Vitale NUZZO, Anna SCHNEIDER, Francesco SUNSERI, Vittorino NOVELLO

8. Genetic characterization of grape varieties in Armenia, by Marina DALLAKYAN, Eva ZYPRIAN, Erika MAUL, Alexander YESAYAN, Nelli HOVHANNISYAN

9. Microsatellite analysis of traditional eastern grapevine varieties and wild accessions from Geisenheim collection

in Germany, by Lidija TOMIC; Lalla Hasna ZINELABIDINE; Ernst RUEHL, Oliver BITZ

10. The results of ampeloghraphic study according of modern approaches of Georgian varieties of grapevine (Vitis

vinifera L.) in the Skra collection (Shida Kartli, Georgia) under frame of COST action FA1003, by Irma MDINARADZE David MAGHRADZE, Ekaterine ABASHIDZE, Ramaz CHIPASHVILI, Larisa VASHAKIDZE

11. Ampelo-genetic screening of promising table and wine grape varieties and putative clones, by Leonid TROSHIN, Alexander MILOVANOV, Andrew ZVIAGIN

12. Ampelographic and genetic characterization of the Israeli grapevine germplasm collection, by Elyashiv DRORI, Yaakov HENIG, Oshrit RAHIMI, Silvia LORENZI, Annarita MARRANO, Hodaya BRAUNER, Osvaldo FAILLA, Maria Stella GRANDO

13. Characterization of the main grapevine cultivars of Albania and Kosovo based on ampelographic and molecular

data, by Ariola Bacu, Ylber Bajraktari, Stela Papa, Fadil Thomaj, Sofia Michailidou, Anagnostis Argyriou.

14. Identification and classification of grapevine cultivars (Vitis vinifera L.) from the Balkan subgroup

(subconvarietas balcanica Negr.) by means of phyllometric descriptors, by Klime BELESKI, Dushko NEDELKOVSKI

SESSION 2 - Genotyping

15. Analysis of VvMybA1 and VvMybA2 genes in grape bud sports, by Adrienn KEREKES, Gabriella DE LORENZIS, Antal SZŐKE, Erzsébet KISS, Osvaldo FAILLA

16. Analysis of grapevine berry colour variants at the DNA level, by Adrienn KEREKES, Antal SZŐKE, Anikó VERES, Kitti TÓTH-LENCSÉS, Pál KOZMA, László KOCSIS, Erzsébet KISS

17. Parentage analysis in Hungarian grapevine cultivars of Seibel/Seyve-Villard origin, by Kitti TÓTH-LENCSÉS, Adrienn KEREKES, Antal SZŐKE, Anikó VERES, Pál KOZMA, Erzsébet KISS

18. Study of VvmybA1 and Vvmyba2 polymorphism in color mutation bud sports of Portuguese cultivars, by Isaura CASTRO, Vanessa FERREIRA, David CARRASCO, Rosa ARROYO-GARCÍA, Virgílio FALCO, Olinda PINTO-CARNIDE

19. Genetic analysis of Myb-related genes in Armenian grapevines, by Kristine MARGARYAN, Gabriella DE LORENZIS, Rouben AROUTIOUNIAN, Osvaldo FAILLA

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20. Preliminary results of marker assisted selection for berry colour in a Nektár x Jacquez progeny, by Kitti TÓTH-LENCSÉS, Adrienn KEREKES, Antal SZŐKE, Bernadett FARKAS LAJTERNÉ, Tamás LÖNHARD, Erzsébet KISS, László KOCSIS

21. VvMybA1 and VvMybA2 genes investigation in Aglianico biotypes, by Gabriella DE LORENZIS, David CARRASCO, Rosa ARROYO-GARCIA, Mara ROSSONI, Giovambattista SIMONE DI LORENZO, Osvaldo FAILLA

SESSION 3 – Phenotyping and Cultivar evaluation

22. Advances in grape phenotyping: pigment characterization by reflectance and theoretical chemistry, by Laura RUSTIONI, Letizia Rocchi, Florent DI MEO, Osvaldo FAILLA, Patrick TROUILLAS

23. VitAdapt: an experimental program to study the adaptation of a large range of Vitis vinifera varieties for

Bordeaux vineyards, by Agnès DESTRAC IRVINE, Diego VERGARA, Lise CAHUZAC, Marie-Agläé FOUGERE, Louise DURAND, Didier TEXIER, Nabil ZIRARI, Serge DELROT, Cornelis VAN LEEUWEN

24. Different susceptibility of Georgian grapevine cultivars for downy mildew, by Nana BITSADZE, Mariam AZNARASHVILI, Ramaz CHIPASHVILI, Annamaria VERCESI, Osvaldo FAILLA, David MAGHRADZE

25. Evaluation of eno-carpological traits in Georgian grapevine varieties from Skra germplasm repository, by Ekaterine ABASHIDZE, Irma MDINARADZE, Ramaz CHIPASHVILI, Larisa VASHAKIDZE, David MAGHRADZE, Laura RUSTIONI, Osvaldo FAILLA

26. Phenotyping results of the PRT051 germplasm collection according the protocols proposed, by the COST action FA1003 by João BRAZÃO, Jorge CUNHA, José EIRAS-DIAS

27. An overview on origin and characterisation of Montenegrin grapevine varieties, by Vesna MARAŠ, Tatjana POPOVIĆ, Anita GAZIVODA, Jovana RAIČEVIĆ, Vesna KODŽULOVIĆ, Milena MUGOŠA, Sanja ŠUĆUR

28. Characterization and adaptation of some Pinot Noir clones to the environmental conditions of Serbian grape

growing regions, by Zorica RANKOVIĆ-VASIĆ, Dragan NIKOLIĆ, Zoran ATANACKOVIĆ, Branislava SIVČEV, Mirjana RUML

29. Characterization of nine most appreciated old autochthonous Vitis vinifera L. cultivars for Romania, by Carmen F. POPESCU, Liviu C. DEJEU, Carmen BEJAN

30. Determination of polyphenols in Armenian grapevines, by Kristine MARGARYAN, Rouben AROUTIOUNIAN, Gagik MELYAN, Osvaldo FAILLA

31. Cluster architecture of old neglected Croatian grapevine cultivars (Vitis vinifera L.), by Goran ZDUNIĆ, Ana MUCALO, Irena BUDIĆ-LETO, Iva TOMIĆ-POTREBUJEŠ, Ivan PEJIĆ, Edi MALETIĆ

32. Adaptive traits of Ukrainian grapevine varieties that used in breeding programs, by Vladimir VOLYNKIN, Alla POLULYAH, Viktor KLIMENKO, Vladimir LIHOVSKOI, Nikolai OLEINIKOV, Svetlana LEVCHENKO, Irina PAVLOVA, Valerii ZLENKO, Zinaida KOTOLOVETS, Inna PYTEL, Natalia ROSHKA (presented by Svitlana GORYSLAVETS)

33. Phenotypic characterization of some Armenian grape varieties, by Anna NEBISH, Gagik MELYAN, Rouben AROUTIOUNIAN

34. Content of resveratrol forms in clone candidate and population of red wine Vranac, by Bojan RADOVIĆ, Vele TEŠEVIĆ, Vesna KODŽULOVIĆ, Vesna MARAŠ

35. The usefulness of OIV descriptors for identification of Vitis sp. seedling populations, by Laszlo KOCSIS, Erzsébet KISS, Kitti TÓTH-LENCSÉS, Adrienn KEREKES, Antal SZŐKE, Radojko PELENGIĆ, Stanko VRSIC

36. Molecular, metabolic and phenotypic characterization and relatedness of four main Albanian grapevine

cultivars, by Endrit KULLAJ, Ariola BACU, Fadil THOMAJ, Frida CARKA, Hipokrat FIKU, Anagnostis ARGYRIOU

37. A case of origin an homonym name for grapevine cultivar in Georgia: the cultivar Moldova was named as

Aladasturi, by David MAGHRADZE, Londa MAMASAKHLISASHVILI, Erika MAUL

38. Genotypisation of grapevine varieties denominated ‘Refošk’/’Refosco’ and ‘Teran’/’Terrano’ (Vitis vinifera L.)

cultivated in Istrian Peninsula and Kras, by Denis RUSJAN, Marijan BUBOLA, Danijela PETRUŠIĆ, Zoran UŽILA, Sanja RADEKA, Danijela POLJUHA, Branka JAVORNIK, Nataša ŠTAJNER

8

39. Israeli germplasm: genotyping and phenotyping of native grapevine, by Denis RUSJAN, Radojko PELENGIĆ, Vladimir MEGLIČ, Barbara PIPAN, Etti OR, Branka JAVORNIK, Nataša ŠTAJNER

40. Differences in seeds phenolic composition and antioxidant properties between three wild grapevines and

between Vitis coignatiae and Vitis vinifera, by Stanislaw WEIDNER, Anna POWAŁKA , Magdalena KARAMAĆ, Anna RYBARCZYK, Angelika KRÓL, Agnieszka MOSTEK, Joanna GRĘBOSZ, Ryszard AMAROWICZ

41. Phenolic compounds, anti-oxidant and anti-inflammatory activity of Georgian, West and Central European

wines, by Premysl LANDA, Zsofia KUTIL, Jan TAUCHEN, David MAGHRADZE, Marie KVASNICOVA, Veronika TEMML, Daniela SCHUSTER, Tomas VANEK

42. Analysis of "SV 12 375" X "Kishmish Moldavskii" progeny to powdery and downy mildew resistance, by Dragoslav IVANIŠEVIĆ, Gabriele DI GASPERO, Nada KORAĆ, Serena FORIA, Goran BARAĆ, Petar CINDRIĆ

SESSION 4 – Wild grapevines & Wild-cultivated relationships and domestication

43. Wild grape (Vitis vinifera L. ssp. sylvestris Gmel.) of the South Armenia, by Gagik MELYAN

44. Characterization and potential use of relic wild grapevines In Extremadura (Spain), by Rafael OCETE, Antonio GALLARDO, María Teresa GUERRA, María del Carmen VIDAL-ARAGÓN, Diego RIVERA, Concepción OBÓN, Maria Ángeles LÓPEZ

45. Ecological characterization of wild grapevine habitats focused on arbuscular mycorrhizal symbiosis, by Rafael OCETE, Ignacio ARMENDÁRIZ, Manuel CANTOS, Domingo ÁLVAREZ, Rosario AZCÓN

46. Sanitary status of the wild grapevine in the South Caucasian region, by David MAGHRADZE, Vugar SALIMOV, Gagik MELYAN, Mirza MUSAYEV, Carlos A. OCETE, Ramaz CHIPASHVILI, Osvaldo FAILLA, Rafael OCETE

47. Identification and characterization of V. vinifera subsp. sylvestris populations, by Anna SCHNEIDER, Paolo BOCCACCI, Paola RUFFA, Daniela TORELLO MARINONI, Luca CAVALLO, Gianfranco ROTTI, Stefano RAIMONDI

48. Allelic variation in the VvMYBA1 and VvMYBA2 domestication genes in natural grapevine populations (Vitis

vinifera ssp sylvestris), by David CARRASCO, Gabriella DE LORENZIS, David MAGHRADZE, Eugenio REVILLA, Alberto BELLIDO, Osvaldo FAILLA, Rosa ARROYO-GARCIA

49. Genotypisation of Tuscan Vitis vinifera subsp sylvestris and relation with local subsp. sativa varieties, by Claudio D’ONOFRIO, Giancarlo SCALABRELLI

50. Origin of grapevine varieties in Crimea and Vitis silvestris classification, by Vladimir VOLYNKIN, Alla POLULYAH (presented by Svitlana GORYSLAVETS)

SESSION 5 – Sustainable conservation

51. Preservation and evaluation of Albanian vine genetic resources, by Frida CARKA, Rajmonda SEVO, Hekuran VRAPI

52. Thermal analysis of grapevine explants during cryopreservation by vitrification method, by Miloš FALTUS, Alois BILAVČÍK, Jiří ZÁMEČNÍK

53. Micropropagation of grapevine (Vitis vinifera L.) seedless cultivar Parvana through lateral bud development, by Gayane MELYAN, Aghvan SAHAKYAN, Avag HARUTYUNYAN

54. Micropropagation and in vitro germplasm conservation of Georgian wild grapevines, by David MAGHRADZE, Rafael OCETE, José Luis GARCÍA, Manuel CANTOS

55. Sustainable management of genetic resources: the characterization of a local grapevine variety (cv Grechetto

rosso) and its own landscape, by Rita BIASI, Elena BRUNORI, Pasquale CIRIGLIANO

92

ECOLOGICAL CHARACTERIZATION OF WILD GRAPEVINE HABITATS FOCUSED ON ARBUSCULAR MYCORRHIZAL SYMBIOSIS

Rafael OCETE1, Ignacio ARMENDÁRIZ2, Manuel CANTOS3, Domingo ÁLVAREZ4, Rosario AZCÓN4

1 Laboratorio de Entomología Aplicada. Facultad de Biología. Universidad de Sevilla. Spain 2 Universidad Fuerzas Armadas- ESPE. Sangolquí, Ecuador 3 Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS). CSIC. Spain 4 Estación Experimental del Zaidín (EEZ). Granada. CSIC. Spain INTRODUCTION – The Eurasian wild grapevine, Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi, is cited as an autochthonous and dioecious relative of cultivated vines. Their main habitats are gallery forests situated along rivers and creeks growing on fluvisols. Fungal diseases and, in minor damage, Phylloxera caused a heavy impact on vineyards and also on wild populations. In relation to wild grapevines, this negative situation has been multiplied by human activities. Due to the absence of human selection, this subespecies can play an important role as phytogenetic resource for the viticulture. There is a lack on the knowledge about the susceptibility of wild grapevine to arbuscular mycorrhizal (AM) association.

AIMS AND SCOPES – The aim of the present work is to confirm the presence of arbuscular mycorrhizal fungi (AM) in the wild grapevine rhizosphere spread in different kinds of soils from 18 wild populations from Spain and France. On the other hand, the accompanying flora, the edaphic characteristics and the presence of parasitic organisms on vines were also determined. MATERIALS AND METHODS – The main supporters of the vines from the accompanying vegetation were determined using botanical keys. Samples of soil of 1kg were collected in 18 populations of wild grapevine situated in river bank forests located in Spain and France. Each sample contained edaphic and root materials from the surface up to a maximum of 40 cm of depth. Rhizosphere soil was used for isolation and multiplication (enrichment) of AM fungi associated to the target plants. MA identification is based on ontogenic and the morphology of the spores, spore formation and the structure of their wall. Size, shape, pigmentation, ornamentation, and supporting hyphae form of occlusion are the main criteria in order to group the spores for the identification of the different species and the use of molecular biology as a complementary tool. RESULTS AND DISCUSSIONS –The main supporters are typical plant species from river-bank forests. The main parasitic species are very homogenous. The main native mite species causing infestation on vines are the erineum strain of Colomerus vitis (Pagenstecher) and Calepitrimerus vitis (Nalepa) (Acari, Eriophyidae). The presence of symptoms caused by imported downy and powdery mildews is very frequent. The total number of identified taxa of AM corresponding to 56, 14 of them were determined up to species level and 32 up to genus. AM community composition presents a marked distribution influenced by the pH of the soils. This study only shows the most generalist species which had sporulated at the same time. In the case of the less cited species is very important to note that some taxa such as Tricispora nevadensis, Gigaspora margarita, Glomus, similar to those from the magnicaule type, highlighting the undescribed species belonging to the Pantano de los Hurones and a variety of taxa identified only up to genus level. Regarding the distribution of species is to highlight that Glomus badium is the most generalist species, isolated from 8 locations from a total number of 18 and Glomus rubiforme represented in 7 locations. Claroideoglomus claroideum was present in 5 locations and Funneliformis mosseae in 3 locations. The most diverse populations were Peñaladros, with16 species and the lowest diversity was found in Agrio river and El Bosque with only one species observed.

CONCLUSIONS AND POSSIBLE APPLICATIONS – According to results obtained in this study, it is necessary to remark the wide representation of the main AM genus. It would be considered an index of the high quality of the ecological values of the considered wild grapevine populations.