COSMOSIL PYE (pyrenylethyl group bonded) and COSMOSIL NPE (nitrophenylethyl group bonded) column show unique retention characteristics based on multiple separation modes such as hydrophobic, charge transfer and - interactions. These columns are recommended for the separation of structural isomers. COSMOSIL COSMOGEL Specialty for Structual Isomers COSMOSIL PYE / COSMOSIL NPE Separation of PAHs Due to the planar pyrene ring structure and strong - interactions, COSMOSIL PYE achieves excellent separation of aromatic isomers. Column size Mobile phase Flow rate Temperature Detection 4.6mmI.D. x 150mm Methanol:Water=90:10 1.0ml/min 30 o C UV 254nm Material characteristics Packing material Silica gel Average particle size Average pore size Specific surface area Stationary phase Main interaction End-capping treatment Carbon content COSMOSIL 5PYE 2-(1-pyrenyl)ethyl group Hydrohobic interaction - interaction Charge transfer interaction Stereo selectivity approx. 18% Sample 1. Benz [a] anthracene 2. Chrysene COSMOSIL 5NPE nitrophenylethyl group Hydrohobic interaction, - interaction Charge transfer interaction Dipole-dipole interaction approx. 9% high purity spherical porous silica 5m approx. 120 approx. 300m 2 /g near-perfect treatment Si H 3 C CH 3 Si H 3 C CH 3 NO 2 PYE NPE Column size Mobile phase Flow rate Temperature Detection Sample 4.6mmI.D. x 150mm A: Water, B: Methanol B 80% B 100% 20min. Linear gradient 1.0ml/min 30 o C UV 254nm 1. Naphthalene 2. Acenaphthylene 3. Axenaphthene 4. Flourene 5. Phenathrene 6. Anthracene 7. Fluoranthene 8. Pyrene 9. 2, 3-Benzofluorene 10. Benz [a] anthracene 11. Chrysene 12. Triphenylene 13. Naphthacene 14. Benzo[a] pyrene 15. Perylene 16. Dibenz [a,c] anthracene 17. Dibenz [a,h] anthracene 1. Benz [a] anthracene 2. Chrysene COSMOSIL 5PYE COSMOSIL 5C18-MS-II COSMOSIL 5PYE Separation of ACHs (Adrenal Cortical Hormones) Column Size Flow rate Temperature Detection COSMOSIL 5PYE 4.6mmI.D. x 150mm 1.0ml/min 30 o C UV 295nm Mobile phase: 80% Methanol Mobile phase: 40% Methanol O HO O CH 2 OH OH O HO O CH 2 OH OH 2 3 Sample 1. Phenol 2. Prednisolone 3. Hydrocortisone COSMOSIL COSMOGEL
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COSMOSIL PYE and NPE - Hichrom · In COSMOSIL NPE, on the other hand, isomers with a strong dipole moment are retained preferentially. Both COSMOSIL PYE and NPE offer special resolutions
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COSMOSIL PYE (pyrenylethyl group bonded) and COSMOSIL NPE (nitrophenylethyl group bonded) column show unique retention
characteristics based on multiple separation modes such as hydrophobic, charge transfer and - interactions. These columns
are recommended for the separation of structural isomers.
COSMOSILCOSMOGEL
Specialty for Structual Isomers
COSMOSIL PYE / COSMOSIL NPE
Separation of PAHsDue to the planar pyrene ring structure and strong - interactions, COSMOSIL PYE achieves excellent separation of
aromatic isomers.
Column size
Mobile phase
Flow rate
Temperature
Detection
4.6mmI.D. x 150mm
Methanol:Water=90:10
1.0ml/min
30oC
UV 254nm
Material characteristicsPacking material
Silica gel
Average particle size
Average pore size
Specific surface area
Stationary phase
Main interaction
End-capping treatment
Carbon content
COSMOSIL 5PYE
2-(1-pyrenyl)ethyl group
Hydrohobic interaction
- interaction
Charge transfer interaction
Stereo selectivity
approx. 18%
Sample 1. Benz [a] anthracene
2. Chrysene
COSMOSIL 5NPE
nitrophenylethyl group
Hydrohobic interaction,
- interaction
Charge transfer interaction
Dipole-dipole interaction
approx. 9%
high purity spherical porous silica
5 m
approx. 120
approx. 300m2/g
near-perfect treatment
SiH3C CH3SiH3C CH3
NO2
PYE NPE
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample
4.6mmI.D. x 150mm
A: Water, B: Methanol
B 80% B 100%
20min. Linear gradient
1.0ml/min
30oC
UV 254nm
1. Naphthalene
2. Acenaphthylene
3. Axenaphthene
4. Flourene
5. Phenathrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. 2, 3-Benzofluorene
10. Benz [a] anthracene
11. Chrysene
12. Triphenylene
13. Naphthacene
14. Benzo[a] pyrene
15. Perylene
16. Dibenz [a,c] anthracene
17. Dibenz [a,h] anthracene
1. Benz [a] anthracene 2. Chrysene
COSMOSIL 5PYE COSMOSIL 5C18-MS-II COSMOSIL 5PYE
Separation of ACHs (Adrenal Cortical Hormones)
Column
Size
Flow rate
Temperature
Detection
COSMOSIL 5PYE
4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 295nm
Mobile phase: 80% Methanol Mobile phase: 40% Methanol
O
HO
OCH2OH
OH
O
HO
OCH2OH
OH
2 3
Sample 1. Phenol
2. Prednisolone
3. Hydrocortisone
COSMOSIL
COSMOGEL
Effect of - interaction
COSMOSIL 5NPE COSMOSIL 5PYE
The ODS column cannot sharply separate the positional isomers like phthalonitrile. In contrast, COSMOSIL PYE and NPE
can do it very well by - interaction. Furthermore, COSMOSIL NPE strongly retains ortho compound which has a big
dipole moment.
o- p-m-
Analysis of disubstituted naphtalenes
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample
4.6mmI.D. x 150mm
PYE: Methanol:Water=90:10
NPE: Methanol:Water=70:30
ODS: Methanol:Water=80:20
1.0ml/min
30oC
COSMOSIL 5PYE
COSMOSIL PYE and NPE columns show very unique retention characteristics for compounds containing electron donors
and acceptors. These substances elute according to their hydrophobicity in ODS columns. In COSMOSIL PYE and NPE,
on the other hand, dinitronaphthalenes are retained more strongly. That means charge transfer interaction contributes to
the retention of dinitronaphthalenes. Furthermore, COSMOSIL PYE retains 1,5-dinitronaphthalene (Sample 2) more
strongly than the 1,8- form (Sample 1), due to the charge transfer interaction. In the COSMOSIL NPE, on the other hand,
Sample 1 is retained more strongly. In addition to - interaction, dipole-dipole interaction is utilized for its retention.
Column size
Mobile phase
Flow rate
The chromatograms below are examples for analysis of the electron donor anisole and the electron acceptors nitroben-
zene and benzene. COSMOSIL PYE, conventional Ph and ODS show different elution orders and reteintion times.
Effect of dipole-dipole interaction
4.6mmI.D. x 150mm
NPE: Methanol:Water=40:60
PYE: Methanol:Water=80:20
ODS: Methanol:Water=30:70
1.0ml/min
Temperature
Detection
Sample
30oC
UV 254nm
1. o-Phthalonitrile
2. m-Phthalonitrile
3. p-Phthalonitrile
(0.30 g)
(3.00 g)
(0.15 g)
CN
CN
CN
CN
CN
CN
Conventional ODS
COSMOSIL 5PYE Conventional Ph
1 32
Column size
Mobile phase
Flow rate
4.6mmI.D. x 150mm
PYE: Methanol:Water=50:50
Ph: Methanol:Water=40:60
ODS: Methanol:Water=50:50
1.0ml/min
Temperature
Detection
Sample
30oC
UV 254nm
1. Nitrobenzene
2. Anisole
3. Benzene
(0.125 g)
(1.50 g)
(4.00 g)
Conventional ODS
NO2 OCH3
(0.21 g)
(0.11 g)
(0.25 g)
(0.35 g)
(0.42 g)
1. 1,8-Dinitronaphtalene
2. 1,5-Dinitronaphtalene
3. Naphtalene
4. 1-Methylnaphtalene
5. 1,5-Dimethylnaphtalene
NO2 NO2
NO2
NO2 CH3CH3
CH3
COSMOSIL 5NPE Conventional ODS
Separation of Tocopherols
COSMOSIL 5PYE COSMOSIL 5NPE Cnventional ODS
Column size
Mobile phase
Flow rate
Temperature
Detection
4.6mmI.D. x 150mm
PYE: 90% methanol
ODS: 95% methanol
1.0ml/min
30oC
UV 295nm
Sample 1. -Tocopherol
2. -Tocopherol
3. -Tocopherol
4. -Tocopherol
Conventional Ph
(min)
Conventional ODS
3
2
1
3 21
3
2
1
T
m-
p-
o-
B
Advantage of dipole-dipole interactions
The chromatogram below illustratews the separation of o-, m-, p-tolunitrile. ODS column cannot achieve complete separa-
tion of them, while COSMOSIL NPE separates them more greatly.
Comparing hydrophobicity, tolunitrile which contains a CN- group is lower in its hydrophobicity than benzene and toluene.
Since hydrophobic interaction is dominant in the separation by a ODS column, tolunitrile elutes first. In COSMOSIL NPE,
tolunitrile elutes later. This suggests that COSMOSIL NPE utilizes the interaction between -electron of the nitrophenyl
group and CN- for retention in addition to hydrophobic interaction.
COSMOSIL 5NPE Conventional ODS
Column size
Mobile phase
Flow rate
Temperature
Detection
4.6mmI.D. x 150mm
Methanol:Water=50:50
1.0ml/min
30oC
UV 295nm
CH3
CN
CH3
Tolunitrile
(o-, m-, p-)
Benzene
(B)
Toluene
(T)
Separation of Dioxines
Polychlorodibenzo-1,4-dioxins (pCDDs) have been in the focus of environmental separations due to their extreme toxicity.
The pCDDs are usually present as a mixture of isomers. The mixture must be separated in order to evaluate the biological
efects of each isomer. The pCDD isomers can be separated by utilizing hydrophobic, charge transfer and dipole-dipole
interactions. COSMOSIL PYE retains symmetrical isomers more strongly. In COSMOSIL NPE, on the other hand, isomers
with a strong dipole moment are retained preferentially. Both COSMOSIL PYE and NPE offer special resolutions that are
beyond the possibilities of conventional ODS columns as shown below.