Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package for use with…

Copyright 2006 by BioTX Automation, Inc.

WellAware™ Forensic WellAware™ Forensic PackagePackage

for use with…


Settings MenuSettings MenuUsed to allow customization for any lab.Used to allow customization for any lab.


WellAware™ Forensic Package Flow WellAware™ Forensic Package Flow DiagramDiagram

WellAware™WellAware™CompleteComplete

ABI 310/3100ABI 310/3100SequencerSequencer

WellAware™WellAware™CompactCompact

ABI 7000ABI 7000RT-PCRRT-PCR


WellAware™WellAware™Forensic PackageForensic Package

1. Samples that have been extracted and are ready for quantification with an AB 7000 Real Time PCR instrument, are entered into the plate form and then printed.

2. The next step is to export a file for the quantification setup in WellAware format. This file is opened in WellAware which then guides the scientist to load the extracted samples into the AB 7000 Real Time PCR plate. WellAware voice and light cues direct the addition of master mix to the wells.

3. In the Forensic Interface, the scientist exports plate format information for running the AB 7000.

4. Once the AB 7000 real time PCR instrument has completed the quantification of samples, the results from the AB 7000 Prism Instrument software are imported back into the Forensic Interface.

WellAware™ Forensic Package How it WellAware™ Forensic Package How it WorksWorks

WellAware™WellAware™CompleteComplete

ABI 7000ABI 7000RT-PCRRT-PCR

WellAware™WellAware™Forensic PackageForensic Package

Plate Form for Plate Form for ABI 7000ABI 7000

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WellAware™WellAware™Forensic Forensic PackagePackage

5. The DNA processing screen allows the scientist to add the samples that have now been quantitated to an AB 310 or AB 3100 plate for electrophoresis and detection. This set up is flexible, and the scientist can run a sample multiple times, and can even override the dilution amount if they choose. Additional sample descriptions, for example case number, can be added under the sample header.

6. Once the scientist has the samples in the order they have specified, they can just click on either the AB 310 or AB 3100 tab, depending upon which platform they would like to set up. After printing the sample set-up sheet, a WellAware file can be created by clicking on the Export Data tab and designating whether to pipette by rows or columns.

7. The exported file is opened in the WellAware software and the scientist can now set up their dilution plate for amplification. WellAware calls out how much dilutant (TE or H2O) to add to each well of the dilution plate. It will then ask for the barcoded extracts to be scanned. Once scanned, WellAware calls out the volume of DNA to add to each well of the dilution plate, and lights up the destination well. Once the sample has been pipetted the footswitch is used to proceed to the next sample.

8. The final step in using the Forensic Interface is in the exporting of the AB 310 or AB 3100 file. The information put into the processing screen for the dilution, step 6, is used to generate the file for the AB 310 or AB 3100 Genetic Analyzers.

WellAware™ Forensic Package How it WellAware™ Forensic Package How it WorksWorks

ABI 310/3100ABI 310/3100SequencerSequencer

WellAware™WellAware™CompactCompact

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Processing ScreenProcessing ScreenNorm & 310/3100 dataNorm & 310/3100 data


WellAware™ Forensic Package WellAware™ Forensic Package Main ScreenMain Screen

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Step 8

Step 4

Step 3


Quant WorksheetQuant Worksheet

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WellAware™ Quant RT-PCR WellAware™ Quant RT-PCR Pipetting Data Pipetting Data (Page 1)(Page 1)

Step 2


WellAware™ Quant RT-PCR WellAware™ Quant RT-PCR Pipetting Data Pipetting Data (Page 2)(Page 2)


WellAware™ Quant PipettingWellAware™ Quant Pipetting

ab

c

Step 3


ABI 7000 real-time PCR Export ABI 7000 real-time PCR Export DataData

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ABI 7000 RT-PCR system ABI 7000 RT-PCR system Normalization DataNormalization Data

Raw data from ABI

7000

Calculated normalization

values

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Normalization ProcessNormalization Process

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Pull down menu

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WellAware™ Normalization WellAware™ Normalization Pipetting DataPipetting Data

Step 7


ABI 310/3100 (Genetic Analyzer) ABI 310/3100 (Genetic Analyzer) WorksheetWorksheet

Step 7

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Contract Engineering Contract Engineering and Softwareand Software

• Utilizes company’s core technology strengths

• Software and engineering

• Customers: MWG, PicoScript, Valco, Sigma-Genosys

Blood card punching robot

Peptide & DNA Synthesizers

Petri dish plating robotCustom PAGE gel rigs


BioTX’s RADS™ Robot BioTX’s RADS™ Robot ToolsTools


BioTX ContactsBioTX Contacts

James Frost [email protected]

Mary [email protected]

16753 Donwick Drive, Suite A6

Conroe, TX 77385936-273-2633

Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package for use with…

Documents

biotx automation

data slide

wellaware file

menu step

final step

wellaware software

rtpcr slide

wellaware format