CONVENTIONAL METHODS CONVENTIONAL METHODS FOR FOR BACTERIAL IDENTIFICATION BACTERIAL IDENTIFICATION Dr Mostafa Mahmoud Ahmed, Ph D Dr Mostafa Mahmoud Ahmed, Ph D Consultant Microbiologist, GDHA, Consultant Microbiologist, GDHA, Riyadh, KSA. Riyadh, KSA. Associate Prof. of Microbiology & Associate Prof. of Microbiology & Immunology. Immunology. Faculty of Medicine – Ain Shams Faculty of Medicine – Ain Shams University University
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CONVENTIONAL METHODS CONVENTIONAL METHODS FOR FOR
BACTERIAL IDENTIFICATIONBACTERIAL IDENTIFICATION
Dr Mostafa Mahmoud Ahmed, Ph DDr Mostafa Mahmoud Ahmed, Ph DConsultant Microbiologist, GDHA, Riyadh, KSA.Consultant Microbiologist, GDHA, Riyadh, KSA.Associate Prof. of Microbiology & Immunology.Associate Prof. of Microbiology & Immunology.
Faculty of Medicine – Ain Shams UniversityFaculty of Medicine – Ain Shams University
Methods for IdentificationMethods for IdentificationA- Phenotypic Characteristics:
3- Immunophenotyping (serotyping): presence of specific antigens (O, H, K,….)
B- Genotyping and Molecular identification
Macroscopic AppearanceMacroscopic Appearance
Growth on Growth on Culture MediaCulture Media
Growth in Fluid MediaGrowth in Fluid Media
Uniform
FlocculentSediment
Colonial MorphologyColonial Morphology
Colonial MorphologyColonial Morphology• Size• Shape• Surface• Pigment production People• Consistency Come• Edge (margin)• Elevation• Opacity Other• Effect on Blood agar are Badly• Lactose fermentation Late
Some
Early
SIZESIZE
• Pinpoint• Small• Medium• Large
The shapes of colonies describes the entire colony, while the margin describes the edges.
Colonies may be circular or regular, irregular and punctiform.
A punctiform colony is too small to describe as either regular or irregular.