Continuous processing and evaluation of experimental data is highly needed 2.1. Organization of work, type of data 2. Helpful hints in the processing of experimental data - Avoid the approach „This year I am experimenting and next year is for the evaluation of what I would obtain“ - Spectrum of software must be managed Type od data: - Numerical data - raw data or data converted to the numerical form, may be obtained with certain tool (ruler) or equipment (spectrophotometer) - Graphical data - very important set of results, their quantification is highly needed - Structural data - analytical data, outcomes from sequencing machines, etc.. B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
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Continuous processing and evaluation of experimental data is highly needed
2. Helpful hints in the processing of experimental data. 2.1. Organization of work, type o f data. Continuous processing and evaluation of experimental data is highly needed. - Avoid the approach „This year I am experimenting and next year is for the evaluation of what I w ould obtain“. - PowerPoint PPT Presentation
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Continuous processing and evaluation of experimental data is highly needed
2.1. Organization of work, type of data
2. Helpful hints in the processing of experimental data
- Avoid the approach „This year I am experimenting and next year is for the evaluation of what I would obtain“
- Spectrum of software must be managed
Type od data:
- Numerical data - raw data or data converted to the numerical form, may be obtained with certain tool (ruler) or equipment (spectrophotometer)
- Graphical data - very important set of results, their quantification is highly needed
- Structural data - analytical data, outcomes from sequencing machines, etc..
B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
Software used in modern laboratory:
- Software controlling machines - sometimes very complex (PCR cycler), the most important is the form of the output (table, text)
- Specialized software - very important in the field of image analysis and processing of sequence data
- General software - text and spreadsheet editors, graphical software, presentation software, mainly products of Microsoft
- Open source software - very good tools, often problems with the compatibility
- online software – mainly in bioinformatics
2. Helpful hints in the processing of experimental data
2.1. Organization of work, type of data
B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
Windows Explorer or similar software (Windows Commander)
Protocols named according to date - the folder in format YYMMDD (081030). Must correspond to date in the laboratory book.
Never modify folder with raw data - copying to the folder with other files accompanying the experiment.
Back up data - external HDD or DVD or CD, online hard drives
Do not delay backuping!!!
Experiment must be always finished with complete documentation, do not start others without finishing the previous.
2. Helpful hints in the processing of experimental data
2.1. Organization of work, type of data
B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
Windows Explorer
2. Helpful hints in the processing of experimental data
2.2. Data explorers, correct data handling and saving
B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
2. Helpful hints in the processing of experimental data
2.2. Data explorers, correct data handling and saving
B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
Windows Commander
2. Helpful hints in the processing of experimental data
2.2. Data explorers, correct data handling and saving
B130P16E: Practical basics of scientific work Department Experimental Plant Biology FSCU http:/lhr.ueb.cas.cz/petrasek
Bulk data processing, their editing and graphical output
Microsoft Excel – suitable for all basic jobs including statistics
Specialized statistical software - in case of need of more parameters, software Statgraphics, NCSS
2. Helpful hints in the processing of experimental data