nsiderations for Protein Crystallograp (BT Chapter 18) owing crystals ly require 0.5mm in shortest dimension, except if u hrotron radiation; Can be “twinned” (two or more cr ing into each other) Why X-rays? e wavelength of radiation comparable with the length interatomic distances.
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Considerations for Protein Crystallography (BT Chapter 18) 1.Growing crystals Usually require 0.5mm in shortest dimension, except if using Synchrotron.
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Considerations for Protein Crystallography (BT Chapter 18)
1. Growing crystals
Usually require 0.5mm in shortest dimension, except if using Synchrotron radiation; Can be “twinned” (two or more crystals growing into each other)
Why X-rays?
The wavelength of radiationis comparable with the lengthof interatomic distances.
Considerations for Protein Crystallography
2. Collecting Diffraction Data
Synchrotron radiation has become routine for structuraldeterminations. The high intensity beam reduces boththe time to collect data and size of crystal needed.
Defining the unit cell of the crystal
Figures 18.6, 18.7 of BT discuss the calculation of the unit cell.
Bragg’s Law 2d(sin) =
is known, and is readily calculated in experimental set-up
Properties of diffracted beam
Each diffracted beam is defined by amplitude, wavelength, and phase.
Amplitude – measured by intensity of recorded spot
Wavelength – set by x-ray source
Phase – major problem in solving crystal structures
Solving the phase
Max Perutz and John Kendrew pioneered molecular isomorphicreplacement (MIR), which utilizes heavy atom derivatives of proteincrystals to introduce new diffraction patterns. Typically use metalssuch as mercury or platinum.
Multiwavelength Anomalous Diffraction (MAD) is extremelypopular but requires synchrotron radiation in addition to a heavymetal derivative. The intensity differences obtained in thediffraction pattern using x-rays of different wavelengths can be usedin a way similar to MIR. The sensitivity permitted by synchrotronradiation allows the use of lighter elements. The most tractable anduseful method has proven to be incorporation of selenomethionineinto expressed proteins.
Structural Genomic Consortia and HTS structure determination
How can I tell if a crystal structure is insightful?
Crystal structures are reported at a variety of resolutions5 Angstroms – can make out secondary structures, but
not individual groups of atoms3 Angstroms – can trace alpha carbon backbone, but
not sidechains1.5 Angstroms – Good resolution
R Factor – Each crystal structure will report this value, which corresponds to error associated with the model; In general,0.2 or lower indicates a well-determined protein structure.
B Factor – Temperature factor, should be 20 or less for good structures. Surface loops or terminal regions often havehigh B values due to flexibility, leading to disorder
Structure from Nuclear Magnetic Resonance
Sample is placed in a strong magnetic field and exposedto radiofrequency radiation.
Energy absorption is characteristic of the nuclei (H1 or C13),and its chemical environment.
Allows structure determination under solution conditions
Some limitation in size, but larger magnets helping