Conformational changes of Gag HIV-1 on a tethered bilayer provide insights into viral assembly Hirsh Nanda, Susan Krueger NIST Center for Neutron Research, Gaithersburg, MD, USA Frank Heinrich, Mathias Loesche Carnegie Mellon, Pittsburgh, PA, USA Siddhartha .A.K. Datta, Alan Rein National Cancer Institute, Frederick, MD, USA
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Conformational changes of Gag HIV-1 on a tethered bilayer provide insights into viral assembly Hirsh Nanda, Susan Krueger NIST Center for Neutron Research,
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Conformational changes of Gag HIV-1 on a tethered bilayer provide insights into viral assembly
Hirsh Nanda, Susan Krueger
NIST Center for Neutron Research, Gaithersburg, MD, USA
Frank Heinrich, Mathias Loesche
Carnegie Mellon, Pittsburgh, PA, USA
Siddhartha .A.K. Datta, Alan Rein
National Cancer Institute, Frederick, MD, USA
Viral Life Cycle
• HIV rapidly develops drug resistance
• Patients are given multi-drug treatments, targeting several stages of the viral life cycle
• No anti-virals target assemblyGag protein mediates viral formation
• Binds other viral components• Self-assembles on the cellular
membrane
Matrix Domain of Gag Binds the Membrane
- - - - -+ + + Matrix (MA): Targets the
membrane - basic residues interact favorably with charged lipids - myristate group inserts in bilayer
Capsid (CA): Lateral organization of Gag proteins on the membrane surface