Conference Manual & Proceedings

Proceedings

The Joint Meeting of Conference and Congress

of Asian Society of Veterinary Pathology (ASVP) 2011

amp ) ~

The 10th Scientific Symposium of Indonesian E Society of Veterinary Pathology (lSVP) 2011

The Role of Veterinary Pathology Animal Health for Improving Eco-Health

IPB International Convention Centre Bogar Indonesia lJovember 2011

Hosted by Faculty of Veterinary Medicine Bogar Agricultural University 2011

Faculty of Veterinary Medicine Bogor Agricultural University 20 I I

All right reserved No part of this book may be reproduced in any form without pennission in writing from the publisher except by reviewer who wishes to quote brief passages in review written for inclusion in magazine or newspaper

Perpustakaan Nasional Indonesia Cataloguing -in-Publication Data The Joint Meeting of the 5th Conference and Congress of Asian Society of Vetrinary Pathology (ASVP) and the 10th Scientific n Symposium of Indonesian Society of Veterinary Pathology (ISVP) 2011 The Proceedings of the Joint Meeting of the 5th Conference and Congress of ASVP and the 10th Scientific n Symposium of ISVP November 22-242011 IPB International Convention Centre Bogor Indonesia ISBN 978-979-493-371-8

Typest and Printed by Penerbit IPB Press

Contact Address ASVP 2011 Organizing Committee Division of Veterinary Pathology Department of Veterinary Clinic Reproduction amp Pathology Faculty of Veterinary Medicine Bogor Agricultural University Wing 6th amp 7th 1st Fl II Agatis Campus IPB of Darmaga Bogor 16680 INDONESIA PhoneFax +62-251-8421807 E-mail asvpbogor2011gmailcom Website httpasvpbogor201Ieventipbacid

Invited Speaker Page OPS 1

OPS2

OPS 3

OP54

OPS 5

OPS 6

OPS 7

POI

P 02

I 03

P 04

I 05

I 06

TGF-beta Niche Regulation for Better Therapy jin-Kyu Park EUI1-lv[i Lee l Ah-Young Kin Sang- Young You Seon-Yolll1~ HOI Elin-Joo Le c and Klll-Shik Jeongl

Pathological findings and Molecular charcteristics ofPRRSV isolated from infected in North of Vietnam Nglyen Thi Lanl Nglllen HUll Na11L Nglyen Ba Hierl Le Huynh Thanh PlIIongL Ngllyen Thi Hoa~ Ryoji YOll1agllchishyRat Tumor Models and their Applications Pariicularly for Renal and Hepatic Fibrosis Jyoji Yalllate Vascular Pathology Associated with Experimental Pastellrela IIlItltocida serotype B2 Infection in Calves

S J(lSni ElY1 AlIIl1(l At Z(JIIri-Sa(d Z Zakario SS Arslwd A R Olwr and T I Allli Challcnges in safety evaluation ofbiomaterials biomedical devices and engineered tissue constructs TV Anikllmar R Deepa (ll1d Al Jaseer Diagnostic Classification and Detection of Minimal Residual Disease (MRD) in Canine Lymphoma ~Blll1gsipipot N 4wwc(i J Clwyapollg S Wangl1uifholl ond S TechollgulIIsl(i The Development of Porcine Reproductive and Respiratory Syndrome Virus Vaccine

Min- Yuan Chid Hui- Ting Chan Yi- Yin Do Pllng-Ling Huang Victor Fei Pang Chian-Ren

Participant Pathology and Veterinary Pathology Challenge-Future Pcrspective

11 A SlIlfatl Ultra structural Characteristic of Blood Cells in Giants Freshwatcr Stingray (Hil(lIfllnt clwopiruya) ASailaslto Y IatlIJra N CI(I1SII(

Analysis of 153 and PCNA Expression in the Genital and Extra genital CTVT Microarray S Jonalltsa K T(lIIgfrogoon P Klifha S Bolflwisollf S iVallgnaitIIII P TOlgkOllOlfana A Rlngsipl)(It S To ngkOlIatto110

Comparing Expression ofHERR-2 in Malignant Feline Mammary Tumors Tissue Microarray tV RlIngrass(lIee P TmlOrnpanich W Jilltowes S Bulfhaisong S Wal1gnaifhlll1l P Tangkmraffmw A Rllngsipipai S TClllgkallaffona Investigation of the Death of Java Rhinoceros (Rhinoceros sondaicus) in Ujung Kulon National Park Adhi Racl1llI(Jf Slidraiat Har~lOdi Halldayani Agls Pr~lOlIIhlldi Ridlmn Seti(JlIII Pathogenicity of a Pathogenic Filed Isolate NDVBali- 1107 in Commercial Chicken Strain ISA brown Anak Agllng Ani vliwh Adi iHade Kardel1a ~rollall Hanfik Asfmi(J Yoshihiro Hayashi YaslIl70hll AlotSlllloto

3

5

8

II

13

15

20

Page 25

26

28

30

35

P07 Immunohistochemical Characterization of Macrophages and 37 Myofibroblasts in Biliary Cirrhosis due to Liver Fluke (Fasciola spp) Infestation in Cattle HM Golbar V Junianfifo C Ichikawa M Tanaka T [ma

Af KlIlIamlira OJ lamale P 08 Expression of Metallothionein Protein in Malignant Feline 40

Mammary Tumors by Using Tissue Microarray P Siffhicharoenchai P Alarlow P Prufthifhmrorn S Sirivaidyapong S Wangnaithllll1 S Tangkallutfana A RUl1gsipiput

P 09 Genetic Characterization and Phy logenetic Analysis of Thai 43 Canine Distemper Virus (CDV) Isolates S TechangamslIln N Charoensiml A Radranakatikanol7 A RlIl1gslJI)(It

PIO In Vitro Antimicrobial Activity of Saccharomyces cerevisae 46 Against Streprococclis agalactiae Isolated from Nile Tilapia K Pinpill1ai P Kaywlsalllrlwj C Rodkhwl1 A POl1pornpisit LV pirarat

PI Necrotizing Granulomatous Lesions of Uterus in a Dog by 48 Nematode Larvae in the family ofOnchocercidae JK Park A Y Killl El Lee lJ Lee SY YOII SY Han DAf KHmiddotak TH Kim KS Jeong

PI2 Phylogenetic and RFLP analysis of Canine Distemper Virus 51 Fusion (F) Gene from Domestic Dogs in Thailand A Ractanakatikanon N Clwroensivu J Kemrchoroen K Oraveeraklil K Sliwolnokaril r Poovorowo1 S Techongwls 11(1

P 13 Immunohistochemical Detection of Macrophages and 54 Myolibroblasts in rat Model of Bleomycin-Induced Scleroderma Vetlliah JllliOlto Cliisa [chikm( Takeshi 1lt7m lv[itslirII Kill ( 11 (Ill Jroji l(II11ate

PI4 High Pathogenic Avian Influenza Infection in Wild Birds of 56 Korea Olin-KlOng 10011 Woo-Hee Park KYlll1g-f~IIn Lee In-Soon Rooh Moo1- Yo ling Rhyoo Hle-Ryoung Kim YOII Chan Bae O-Soo Lee

P 15 Ganglioneuritis of Indonesian Abalone 57 Dewi Ratill AglIllgprimno MmaISllballgkit Agus SlIl1alto

P 16 Canine Multiple Epithelioid Hemangioma in Labial Mucosa 59 Elv Lee A Y Kil1l EJ Lee BS Joo SY YOII SY Han YH YOOI1 KS Jeong

PI7 Histopathological Changes and Apoptosis Detection in Canine 62 Myxomatous Mitral Valve Disease Using Tissue Microarray Technique T Jiranantasak A Rungsipipat S Disatian

PI8 Case Report Infectious Canine Hepatitis and Colibacilosis in 65 Malayan Sun Bear (Hearcos Afalayanlls) Agustin [ndrmafi

P 19 Generation of Hepatocellular Carcinoma animal Model in 67 Sparoglle Dmrfey Rats Aglls SetilOl1o [ndriayzmi Prahastllti Elpita Br Tarigall fJkterono Dlti BlI(Zvati Andi [llama

P 20 Case Report Ovarian Lipoma in a Dog 70 JH Park A Y Kim EJI Lee sr Yo II SY Han EJ Lee BS 100 AI Mlln Ill Yoon KS Jeong

P 21 Decreased Myostatin Moves Up Fetal Myogenesis in Somatic 72 Cell Nuclear Transferred Dog EJ Lee JK Park A Y Kim EAt Lee Sr ron sr Hall YH Yoon BS 100 JH Park AI AlII KS Jeong

P 22 Ceruminous Adenocarcinoma with Psammoma Bodies in 75 External Auditory Canal of a Dog A Y Kim Ev1 Lee SY YOIl SY Han YR Yoon KS Jeong

P 23 Production and Characterization of Plyclonal Anti Domain-I 78 Alpha-Fetoprotein Antibody AglIs Setirolo Elpita BI Tarigan IlldriaYlini Pralwsfufi Akferono Dwl Blldmfi Andi Ufallla

P 24 Pesticide Poisoning in Korean Wild Birds from 2008 to 20 II 81 OIlI1-Klolllg Moon Moon- rOllng Rhyoo Kyung-HllIl1 Lee InshySoon Roh Woo-Hee Park Dong-GYII Kim Jin- YOllllg Shin HYIlI1-Jeong Kl1lil Clwe-III Lim O-Soo Lee

P 25 The Effect of Blackseed (Nigel([ satim) Oil Extracts on Mice 82 (Mus musculus) Testicle Histopathology A Rahllli DR AglI1gpr(I0I10 S Estllnlllgsiit

P 26 Splenic Angiomyxoma in a Dog The First Reported Case 85 BS Jo A Y Kim Elf Lee SY YOll SY Hcm EJ Lee JR Park AL MIIII YR YOOII KS Jeong

P27 A Case of Hemangiosarcoma in a Jindo Dog H7 AL MIII A Y Kim EM Lee SY Yo II St Han fJ Lee BS JO() JR Pork KS Jeong

P 28 Foot and Mouth Disease Virus Infection in Young Korean Black Goat (Capra itirclIs) in Korea KIIIIg-Hmn Lee In-Sool1 Roil Woo-Hee Park MoolI- YOllllg RtlOo YOlIlIg-JOOII Ko JOllg-Hleoll Pork OIlIl-KnJllng Mool O-Soo Lee

P 29 Acute Severe Melena Caused by Salmonella Paratyphii C 90 Infection in Javan Slow Loris Nycticebus Javanicus (A Case Report) [fall Citronillgplltri S(rlllilli JP Katee S rVendi P

P 30 A Case of Bacterial Septicaemia in a Malayan Tapir (Tapirlls 92 indiclls) Sr(lIlo S S(illldill vI ZWllri-Saad

P 31 Effect of Garlic (Allilllll solmill L) to the Immunity of Carp 94 Fish (ClprillllS (lII1)io) Infected by EdHordsiela tonfa Kllmi(sih Syaitiudin Toto The Application of Immunohistochemistry to Detect Prion 96 Protein (Prp) of B(mlIe Spongitiml El7cepjaiopafhy (BS E) in Feedlot Cattle to Anticipate BSE Emergence in Indonesia Rilli )unwyanti

P 33 Mammary Adenocarcinoma with Pan-Cytokeratin and Alpha 98 Estrogen Recepto Immunohistochemical Detection in Captive African Hedgehog (AtelerJ~( ahitellfis) K(tr~a ClwnkOlI KOlllkiell Pinpimai Poison Tielltwi Nopadul1 Pirarof

P 34 The Suspect Atlatoxicosis in Toxicity Case Caused by 100 Aspergillus Fungi WohYllni Hadi PUrlWIIO ~f

P 35 Gross Pathology Report of a Wild-Born Captive Sumatran 102 Rhino (Dicerorhinlls Sli 17 W frellS is ) Torgamba DR Agul7gpr~lOlIo AI Suhangkil S EStlll1illgsih D Condra 4ndricll1syah

P 36 Pulmonary Lesions of an Aged Sumatran Rhinoceros 105 Wilrin Wil7osih Vetniah Junianto DeHi Rati Agungpn)Ono Dacwl D SlIbrata

P 37 Widespread Fibrosis Due to Uremia in an Aged Sumatran 107 Rhinoceros (Dicerorhinlls slIl1Iatrensis) Vetniah JlIl1ianfo Aglis SelilOno BalJlbong Pontjo Priosoeranto Al1drial1s~h

PANCREATIC BETA CELLS EVALUATION AFTER TREATED BY Plwleria macrocarpa (Mahkota Dewa) FRUIT EXTRACT IN DIABETIC MONKEY

E Sulistiawati I H Suparto lbull2

M Bintang1 1 Indraswaril SA Prabandari l

Iprimate Research Center Bogor Agricultural University Department of Chemistry and 3Department of Biochemistry Faculty of Mathematics and Natural Science

Bogor Agricultural University Keyword Phaleria macrocarpa Diebetes Mdlitus acacaascicularis

Introduction Phaleria macrocarpa is a medicinal plants

originated from Papua Empirically it is capable to control arious health problems including diabetes mellitus There is growing evidence that excess generation of highly reactive free radicals largely due to oxidative stress (hyperglycemia) causing increase blood level This further exacerbates the development and progression of diabetes and its complications Based on previous studies Phaleria macrocarpa contained antioxidant of phenolic glycoside (Oshimi et al 2008) and lignans pinoresinol lariciresinol and matairesinol (Saufi et aI 2008) The aim of this study was to evaluate the numhcr of heta cell pancreas and to detect antigenshyantihody reaction of ~ cells after treated Phaleria macrocarpa fruit extract in diabetic monkey (DM) induccd with streptozotocin (STZ)

Materials and Methods Pancreatic tissues from fifteen diabetic adults

male Macaca fascicularis were collected at necropsy and preserved in 4 paraformaldehyde as fixative solution Each pancreatic tissues were trimmcd at three difTercnt areas caput corpus and cauda processed for immunohistochemical staining then evaluated and calculated under microscope Monkeys werc induced by single intravenous injection of STZ (55 mgkg BW) to be DM All DM were divided randomly into threc groups (n = 5 anilllals) First group DM treated only with distilled water as control second and third groups were treated with Phaleria macrocarpa fruit extract of 1000 and 500 mgkg BW respcctively All experimental procedure on theses animals were conducted in compliance with the guideline estahlished hy the Institutional Animal Care and Use Committee

Results Pancreatic l3 cells of the Langerhans Islets

distributed randomly in three different areas caput corpus and cauda pancreas The mean of number l3 cells on the caput pancreas of the control DM was higher than both of treated DM group While the mean of the number l3 cells on the corpus and cauda

pancreas of DM which had received extract of 500 mgkgBW was higher than the control and animals with 1000 mgkgI3W (Figure 1)

Immunohistochemical staining method showed various color intensity which depends on the concentration of antigen-antibody binding tissue preparation and other factors In this study the hrown color intensity indicated the amount of insulin that sccreted by pancreatic l3 cells Factors afTccting the color intensity of the antibody-antigen binding specificity closely related to the concentration of primary antibody used in the process Optimization of anti-insulin concentration was performed and revealed that the concentration of I 1500 as the optimal concentration with unstained background supported by Gobel (20 II)

I3ascd on the data of the color intensity of pancreatic ~ cell evaluation the caput pancreas of the control DM had the lowest of color intensity compared to both treated groups Corpus and cauda areas of animals treated with 500 mgkg BW showed highcr intensity compared to the other groups (Figure 2)

Disscussion Detection of pancreatic l3 cells in Langerhans

Islet hy immunohistochemistry indicate the presence of ~ cell damage in diabetes mellitus Pancreatic tissue of untreated animals had lowest pancreatic l3 Ct~11 compared to treated animals This may he caused hy adanced ~ cell damaged due to cytotoxic cffect of STZ Glucose uptake through glucose transporters (GLUT-2) caused the STZ into ~ cdls resulting in DNA alkylation DNA was fiagmented so that activating poly (ADP-ribose) synthetase the enzyme that polymerizes to form the ADP-rihose poly (ADP-ribose) and activation uf the ATP and NAD+ reduction Decreased production of ATP and NAD+ led to the opening of K+ channels and the plasma membrane hyperpolarization Furthermore closure of the gate voltage reduced Ca2+ concentration and insulin secretion resulting in l3 cell death (Elsner et al 2000)

1000 500

Group

bull Caput bull Corpus Cauda

Control

Figure I Mean of the number of p cells on three different areas of pancreas by immuno histochemical staining evaluation

Fruit extract improved total number of p cell modestly higher in the lowest dosage (500 mglkg BW) This was supported by Supa-rlo CI 01 (20 I0) that Phaleria macrocarpa tjuit etract increased insulin secretion on diabetic cynomolgus monkeys This fruit extract was already available in the market with dosage of 500 mgcapsule It revealed that dose of 500 mgkgBW was more appropriate to improve hyperglycemic condition or to reduce blood glucose leve

Pancreatic p cells distribution in three different areas of untreated animals showed that the caput and the corpus pancreas had higher p cells than the cauda pancreas In the highest dosage the corpus area showed higher numbers of Pcells than in the caput and caudal pancreas Total number of p cells for the lowest dosage showed higher number in the corpus and the cauda compared to caput pancreas This result was supported by Oahmiarti (2000) that the distribution of diabetic pancreas p cells located at the periphery of thc islets of Langerhans However according to Sundler and Harkanson (1988) P cell distribution diflcred from each species and the composition of the islets of Langerhans dif1ered from each area of the pancreas The Langerhans islets located in the cauda pancreas seemed II) has tendency to be more numerous

The color intensity of pancreatic p cells were highest in treated animal with 500 mgkg BW This result was also indicated by increasing in the number of pancreatic P cells J dark brown color on the Langcrhans Islets was resulted from the aflinity of anti-insulin with high insulin and vice versa Affinity of antibody was the strength of the bond of a side of antibody binding (paratop) with antigenic detenninants (epitopes) (Vara 2005) So it can be stated that the number of pancreatic ~ cells vas directly proportional to the color intensity obtained from the reaction of antigen and antibody binding by immunohistochemical staining methods

Conclusion Phaleria marcocarpa fruit extract with dosage

of 500 mglkgBW orallY was the best dose to improve pancreatic beta cells damage in DM

3

-5 a 2

bull Caput 5 5

bull corpus10 IliiCaudau shy

0 r~--~r~-~ -Control 1000 500

Group Figure 2 Mean ofthe color intensity of Pcells

on three different area of pancreas by immuno histochemical staining evaluation

Acknowledgement This study was partly supported by the Ministry

of Research and Technology fund (RT2009-1388) and partly by internal research funds of Primate Research Center of Bogor Agricultural University

References

Elsner M Guldbakke B Tledge M Munday R Lenaen S 2000 Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin Diabctologia43 1528-1533

Gobel S 20 II Optimasi anti-insulin pada sel ~ pancreas Macaw fascicularis secara imunohistokimia [Laporan] Bogor Program Diploma Institut Pertanian Bogor

Harmanto N 2003 Afahkola Dcwa Dbat Pusaka Para Dewa Jakarta Agromedia Pusaka

Oshimi S Zaima K Matsuno Y Hirasawa Y Iizuka r Studiawan H Indrayanto G Zaini NC Morita H 2008 Studies on the constituents from the fruits of Phaleria macrocOlpa Nat Med(Tokyo) 62(2) pp 207-210

Saufi A von Heimendah cB Alfermann AW Fuss E 2008 Stereochemistry of lignans in Phaleria macrocarpa (Schell) Boer Z Nalurforsch 63(1-2) Pl 13-16

Suparto IH et at 2009 Phaaia macrocarpa fruit extract as insulinotropic agent in streptozotocinshyinduced diabetic cynomolgus monkeys (Macaca fascicuaris) Oi dalam Proceeding of International Conference on Medicinal Plants Surabaya 21-22 Juli 20 I O

Sundler J Hakanson R 1988 Handbook of Chemical Neuroanatomy Vol 6 The peripheral Nervous System Elsevier Science Publisher BV

Vara JAR 2005 Technical Aspects of Immuohistochemistry [Terhubung Berkala] httpvetsagepupcomcontentl424405 [03 Mei 20 II J