Korean Journal of Microbiology (2019) Vol. 55, No. 4, pp. 432-435 pISSN 0440-2413 DOI https://doi.org/10.7845/kjm.2019.9136 eISSN 2383-9902 Copyright ⓒ 2019, The Microbiological Society of Korea Complete genome sequence of probiotic Lactobacillus johnsonii IDCC9203 isolated from infant feces Tae-Yoon Kim, Byeonghun Lee, Hyuk-Sang Kwon, Jin Seok Moon * , and Sung Ku Choi Research Laboratories, ILDONG pharmaceutical Co., Ltd., Hwaseong 18449, Republic of Korea 유아 분변에서 분리한 프로바이오틱 Lactobacillus johnsonii IDCC9203 의 유전체 염기서열 해독 김태윤 ・ 이병훈 ・ 권혁상 ・ 문진석* ・ 최성구 일동제약 중앙연구소 (Received November 4, 2019; Revised November 13, 2019; Accepted November 13, 2019) *For correspondence. E-mail: [email protected]; Tel.: +82-31-371-2896; Fax: +82-31-371-2900 Lactobacillus johnsonii IDCC9203 (= KCTC 10923BP) is a probiotic with anti-inflammatory properties, both in vitro and in vivo. Here, we report the complete genome sequence of the IDCC9203 strain, which was isolated from infant feces. The genome was determined using PacBio and Illumina sequencing. This strain comprises a circular chromosome of 1,898,461 bp, and its guanine-cytosine (GC) content is 34.70%. These results will provide an understanding of this strain’s functional properties and safety assessments. Keywords: Lactobacillus johnsonii, anti-inflammatory, infant feces, probiotics Lactobacillus johnsonii (Lb. johnsonii) is one of the many microorganisms that resides in the human intestine. Its beneficial properties, which include immune-modulation (Kingma et al., 2011) pathogen inhibition (La Ragione et al., 2004), enhancement of the epithelial barrier function (Liu et al., 2015), and have been extensively reported. Lb. johnsonii IDCC9203 was isolated from infant feces. This strain substantially inhibit the release of inflammatory mediators such as tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), and IL-1β stimulated the treatment of RAW 264.7 macrophages with lipopolysaccharide (LPS) (Moon et al., 2017) and improved the symptoms of dextran sodium sulfate (DSS) - induced colitis in mice (Je et al., 2018). Despite the above biotechnological applications of IDCC9203, the genome sequence of this strain is unknown. Thus, we analyzed the genome sequence of IDCC9203, and investigated the genetic characteristics of this strain. Lb. johnsonii IDCC9203 was grown in Man, Rogosa, and Sharpe (MRS, Difco Laboratory) broth or agar medium at 37°C for 18 h. The culture was maintained in 30% glycerol solution at -70°C until needed. The genomic DNA of IDCC9203 was extracted from cells after cultivation in MRS medium at 37°C using a Wizard Genomic DNA Purification Kit (Promega), following standard protocol recommended by the manufacturer. Sequencing was performed at the Macrogen using the Pacific Biosciences (PacBio) RSII SMRT and Illumina sequencing technologies. First, the PacBio RS II sequencing was used to generate a 20-kb SMRTbell library. Assembly of the library using the HGAP3 pipeline yielded one contig. Subsequently, Illumina sequencing was performed using an Illumina HiSeq sequencer, and the Canu software (version 1.4) (Koren et al., 2017) was used to assemble the Illumina library. After assembly, HiSeq reads were subjected to final polishing to ensure genome
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Complete genome sequence of probiotic Lactobacillus johnsonii432-435]KJM19-136.pdf · 2019-12-26 · ( oud.net) (Yoon et al., 2017). Virulence factors and antibiotic resistance genes
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Korean Journal of Microbiology (2019) Vol. 55, No. 4, pp. 432-435 pISSN 0440-2413DOI https://doi.org/10.7845/kjm.2019.9136 eISSN 2383-9902Copyright ⓒ 2019, The Microbiological Society of Korea
Complete genome sequence of probiotic Lactobacillus johnsonii
IDCC9203 isolated from infant feces
Tae-Yoon Kim, Byeonghun Lee, Hyuk-Sang Kwon, Jin Seok Moon* , and Sung Ku Choi
Research Laboratories, ILDONG pharmaceutical Co., Ltd., Hwaseong 18449, Republic of Korea