Korean Journal of Microbiology (2019) Vol. 55, No. 3, pp. 309-312 pISSN 0440-2413 DOI https://doi.org/10.7845/kjm.2019.9099 eISSN 2383-9902 Copyright ⓒ 2019, The Microbiological Society of Korea Complete genome sequence of Comamonas sp. NLF-7-7 isolated from biofilter of wastewater treatment plant Dong-Hyun Kim 1 , Kook-Il Han 2 , Hae Jun Kwon 1 , Mi Gyeong Kim 1 , Young Guk Kim 1 , Doo Ho Choi 1 , Keun Chul Lee 2 , Min Kuk Suh 2 , Han Sol Kim 2 , Jung-Sook Lee 2,3 * , and Jong-Guk Kim 1 * 1 School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic of Korea 2 Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea 3 University of Science and Technology (UST), Daejeon 34113, Republic of Korea 폐수처리장의 바이오 필터로부터 분리된 Comamonas sp. NLF-7-7 균주의 유전체 염기서열 해독 김동현 1 ・ 한국일 2 ・ 권해준 1 ・ 김미경 1 ・ 김영국 1 ・ 최두호 1 ・ 이근철 2 ・ 서민국 2 ・ 김한솔 2 ・ 이정숙 2,3 * ・ 김종국 1 * 1 경북대학교, 2 한국생명공학연구원 생물자원센터, 3 과학기술연학대학원대학교 (Received August 27, 2019; Revised September 17, 2019; Accepted September 17, 2019) *For correspondence. (J.G. Kim) E-mail: [email protected]; Tel.: +82-53-950-5379; Fax: +82-53-950-5379 / (J.S. Lee) E-mail: [email protected]; Tel.: +82-63-570-5618; Fax: +82-63-570-5609 Comamonas sp. NLF-7-7 was isolated from biofilter of waste- water treatment plant. The whole-genome sequence of Comamonas sp. NLF-7-7 was analyzed using the PacBio RS II and Illumina HiSeqXten platform. The genome comprises a 3,333,437 bp chromosome with a G + C content of 68.04%, 3,197 total genes, 9 rRNA genes, and 49 tRNA genes. This genome contained pollutants degradation and floc forming genes such as sulfur oxidization pathway (SoxY, SoxZ, SoxA, and SoxB) and floc forming pathway (EpsG, EpsE, EpsF, EpsG, EpsL, and gly- cosyltransferase), respectively. The Comamonas sp. NLF-7-7 can be used to the purification of wastewater. Keywords: Comamonas sp. NLF-7-7, Illumina HiSeqXten, PacBio RS II, wastewater Large scale of farm cause large scale of wastewater. This wastewater need to pass through wastewater treatment plants and purified. But the water purification process causes a huge amount of odor pollutants. Biofilters are responsible for removing these odor pollutants by specific microorganisms which can degrades pollutants (Wani et al., 1997). A sulfur oxidizing bacteria investigation performed that presence of a SoxB gene indicate presence of the Sox pathway and this means presence of sulfur oxidization ability in organism (Petri et al., 2001; Anandham et al., 2008). During the study of analyzing kinds of cultivable bacteria in Biofilter of the wastewater treatment plants, a novel bacterial strain NLF-7-7 was isolated. Based on phylogenetic, phenotypic and chemotaxonomic data, strains NLF-7-7 (= KCTC 62943) was identified as a novel species as a member of the genus Comamonas within the family Comamonadaceae of Betaproteobacteria. The genus Comamonas, proposed by De Vos et al. (1985), is Gram-negative, aerobic, motile, rod shaped bacteria. And the Comamonas sp. NLF-7-7 is Gram-negative, aerobic, non-motile, rod shaped, and floc forming bacteria. Here we describe the complete genome
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Complete genome sequence of Comamonas sp. NLF-7-7 isolated … · 310 ∙ Kim et al. 미생물학회지 제55권 제3호 Table 1. General features of Comamonas sp. NLF-7-7 Property
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Korean Journal of Microbiology (2019) Vol. 55, No. 3, pp. 309-312 pISSN 0440-2413DOI https://doi.org/10.7845/kjm.2019.9099 eISSN 2383-9902Copyright ⓒ 2019, The Microbiological Society of Korea
Complete genome sequence of Comamonas sp. NLF-7-7 isolated from
biofilter of wastewater treatment plant
Dong-Hyun Kim1, Kook-Il Han
2, Hae Jun Kwon
1, Mi Gyeong Kim
1, Young Guk Kim
1, Doo Ho Choi
1, Keun Chul Lee
2,
Min Kuk Suh2, Han Sol Kim
2, Jung-Sook Lee
2,3* , and Jong-Guk Kim1*
1School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic
of Korea2Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic
of Korea3University of Science and Technology (UST), Daejeon 34113, Republic of Korea
폐수처리장의 바이오 필터로부터 분리된 Comamonas sp. NLF-7-7 균주의
유전체 염기서열 해독
김동현1 ・ 한국일
2 ・ 권해준
1 ・ 김미경
1 ・ 김영국
1 ・ 최두호
1 ・ 이근철
2 ・ 서민국
2 ・ 김한솔
2 ・ 이정숙
2,3* ・ 김종국1*
1경북대학교,
2한국생명공학연구원 생물자원센터,
3과학기술연학대학원대학교
(Received August 27, 2019; Revised September 17, 2019; Accepted September 17, 2019)