Competition between budding and tubular formation in vesicles enclosing aqueous polymer solutions Yonggang Liu 1 Theory & Bio-systems, Max Planck Institute of Colloids and Interfaces, Germany 2 State Key Laboratory of Polymer Physics and Ch emistry, Changchun Institute of Applied Chemist ry, Chinese Academy of Sciences (CAS)
18
Embed
Competition between budding and tubular formation in vesicles enclosing aqueous polymer solutions Yonggang Liu 1 Theory & Bio-systems, Max Planck Institute.
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Competition between budding and tubular formation in vesicles
enclosing aqueous polymer solutions
Yonggang Liu
1 Theory & Bio-systems, Max Planck Institute of Colloids and Interfaces, Germany
2 State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy
Macromolecular Crowding within Cell.The cytosol of Escherichia coli contains about 0.3-0.4 g/mL macromolecules.[Zimmerman SB, Trach SO J. Mol. Biol. 222(3), 599–620 (1991).]
4
Background & Motivation
www.wikipedia.org
Cell synthesize a large number of macromolecules (for example protein), which were sorted and transported with the help of vesicles.
Membranes of endoplasmic reticulum and Golgi apparatus have a rather complex architecture consisting of both sheet-like and tube-like membrane structures.
5
Vesicles Enclosing ATPS as Model Cell System
PEG 8k, Rg=4nm Dextran 500k, Rg=21nm
6
Membrane transformations
R. Dimova, R. Lipowsky, Soft Matter 2012, in press.
Budding versus tubular formation.properties of membrane and membrane-polymer interaction (bending rigidity, spontaneous curvature, wetting, tension)
7
Multi-Component Vesicles
S. L. Veatch, S. L. Keller, Biophys. J., 2003, 85, 3074–3083.
R. Lipowsky, et al. Europhys. Lett., 1998, 43, 219-225.
17
Conclusions1. The competition between budding and tubular formation, is resulting from the competition of the bending energy and the interfacial tension.
2. For vesicles enclosing aqueous polymer solutions, both PEG and dextran were depleted from the membrane, inducing a negative spontaneous curvature.
18
Acknowledgement
Prof. Reinhard LipowskyDr. Rumiana DimovaDr. Halim Kusumaatmaja