Comparison of Two Different Sprint Interval Work to Rest Ratios on Acute Metabolic and Inflammatory Responses Harnish CR, RT Sabo, DM Morries, EO Acevedo.

Comparison of Two Different Sprint Comparison of Two Different Sprint Interval Work to Rest Ratios on Acute Interval Work to Rest Ratios on Acute

Metabolic and Inflammatory Metabolic and Inflammatory ResponsesResponses

Harnish CR, RT Sabo, DM Morries, EO Acevedo

This research was made possible through the support of Award Number UL1RR031990 from the National Center for Research Resources

IL-6

IL-6

AMPK↓Glycogen

↑[Cai2+]

Motor UnitRecruitment

IL-10

↑Glucose Uptake

↑Lypolysis AMPK Insulin

Cell

InactiveGLUT-4

IL-6

IL-6

IL-6

IL-6

IL-6

↑↑SSii

oror

GlucoseGlucoseUptakeUptake

TNF-α

Stimulation

Inhibition

Translocation

FunctionalGLUT-4

Adapted from Febbraio and Pedersen 2002; Pedersen and Fischer 2007

Initiation of muscle-derived IL-6 release and the subsequent cascade effect

From Cytokines to Myokines

RECOVERY

Sprint Interval TrainingSprint Interval Training

2 weeks SIT enhanced Si and metabolic functionBabraj, 2009; Gibala 2011; Little 2012

6 weeks 30 sec Wingate sprints elicited similar adaptations to endurance

Weekly volume 225 vs 2250 kJ/wkIncreased enzymes and mitochondria

Burgomaster, 2008; Gibala, 2006, 2008, 2009

Inconclusive single session data on Si or CytokinesBrestoff et al.2010 showed no acute impact of 5 sprints, raising questions about mechanism for chronic improvementWork to rest ratio believed to impact

exercise-stress responseNote: Only 8 of 12 subjects could be analyzed for cytokine response.

Is there a relationship between inflammation and Si?

Will shorter rests influence either inflammation or Si?

QuestionsQuestions

MethodsMethods13 Men & 2 women

23.8 (0.9) yrs, 78.5 (1.6) kg, 1.8 (0.27) m16.9 (1.7) % fat237 (14.6) 2 min Max Watts

Testing completed over 3 – 4 wk; 0700-1100 hrs12 hr fast

Each subject completed both sprint sessionsTabata: 10X 20 sec sprint with 10 sec recovery – 5%

Wingate: 5X 30 sec sprint with 4 min rest – 7%

Study Design

PREBLOOD

PREBLOOD

POST BLOODPOST

BLOOD1 hr POST

BLOOD1 hr POST

BLOOD24 hr POST

BLOOD24 hr POST

BLOOD

RESULTSRESULTS

TABATA WINGATE% Max Power 95.6 (4.7) 196.8 (4.7)

kJ 64.7 (3.5) 68.0 (3.5)

BLC (mM) 12.8 (0.7) 12.6 (0.7)

Total Time 04:50 18:30

IL-10 (pg/ml)Post

1 hr Post

341.5 (37.7)534.5 (37.7)322.2 (37.7)

341.5 (37.7)528.7 (37.7)371.7 (37.7)

TNF-α (pg/ml)Post

1 hr Post

625.6 (76.5)947.4 (76.5)507.6 (76.5)

625.6 (76.5)946.6 (76.5)551.5 (76.5)

Significant p < 0.05; 2X4 ANOVA Significant p < 0.05; 2X4 ANOVA

Inflammatory response to Tabata and Wingate

**

Insulin Sensitivity before and after SIT

RESULTSRESULTS

PREPREPREPRE TABATATABATATABATATABATA WINGATEWINGATEWINGATEWINGATE

Cederholm Index for peripheral insulin sensitivity, 1990

p < 0.03

p < 0.67

Mg

*I2*m

M-1*m

U-1*m

in-1

ConclusionsConclusions

Single SIT session does not improve Insulin Sensitivity 24 hrs post exercise

SIT Pro-inflammatory?Increased IL-10 ineffective at improving Si

W:R does not affect SIT inflammatory responseData indicate IL-10 and TNF myokines

Future Work: When does Si begin to improve after SIT? Between 1 day & 14 days?

Special ThanksSpecial Thanks

Shirley HelmShirley Helm

VCU CCTR and CRSU StaffVCU CCTR and CRSU Staff

Experiment.comExperiment.com

Dept of Health & Human PerformanceDept of Health & Human Performance

IL-1

0

Pearson Correlation: TNF-α vs IL-10

TNF-α

R= 0.79R= 0.79

RESULTSRESULTS

p < 0.0001p < 0.0001