Malviya Reeta et al, 2015/ Comparative evaluation of analgesic activity of Oleogumresin International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (5) 01- 09 1 RESEARCH ARTICLE International Research Journal of Pharmaceutical and Biosciences Pri -ISSN: 2394 - 5826 http://www.irjpbs.com e-ISSN: 2394 - 5834 Comparative Evaluation of Analgesic Activity of Oleogumresin and Stem bark extract of Guggulu in Albino rats. Malviya Reeta 1* , Sharma Ravindra 2 , Sharma Tarun, 3 Ramamurthy A, 4 and Kotecha Mita 5 * 1 Dept. of Dravya Guna, Mahatma Jyotiba Fule Ayurvedic college, chomu, Jaipur, Rajasthan. 2 Dept. of P.G. Studies in Dravya Guna, National Institute of Ayurveda, Jaipur, Rajasthan. 3 Dept. of P.G. Studies in Dravya Guna, National Institute of Ayurveda, Jaipur, Rajasthan. 4 Dept. of P.G. Studies in Dravya Guna. National Institute of Ayurveda, Jaipur, Rajasthan. 5 Dept. of P.G. Studies in Dravya Guna. National Institute of Ayurveda, Jaipur, Rajasthan. Article info Abstract Article history: Received 25 AUG 2015 Accepted 02 SEP 2015 *Corresponding author: [email protected]Copyright 2015 irjpbs Background: Guggulu (commiphora wightii Bhandari) is a very important plant widely used for its antihyperlipidaemic, analgesic and anti- inflammatory actions by both Ayurvedic contemprory medicinal practionars. The Oleo-gumresin is a very widely used raw material of this medicinal plant and it is commonly used in Vata roga and Medo roga in Ayurveda. Objective: The current research paper provides a detailed report of comparative study the Analgesic activity of Guggulu resin & its stem bark extract in albino rats. Methods: An artery clip is applied to the root of the tail of mice and the reaction time is noted. Male mice with a weight between 18 and 25 g are used. The Control Group, Standard Group and Test Group consist of 6 mice in each. The test compounds are administered orally to fasted animals. The drug is administered 15, 30 or 60 min prior testing. An artery clip is applied to the root of the tail (approximately 1 cm from the body) to induce pain. The animal quickly responds to these noxious stimuli by biting the clip or the tail near the location of the clip. A stopwatch in 1/10 seconds increments measures the time between stimulation onset and response. A cut – off time will determine by taking the average reaction time plus 3 times the standard deviation of combined latencies of the control mice at all times periods. Any reaction time of the best animals which greater. Then the cut-off time is called a positive response indicative of analgesic activity. The length of time until response indicates the period of greatest activity after dosing. An ED50 value is calculated at the peak time of drug activity. Results: All three test sample provided significant effect as Analgesic agent. Conclusion: It can be concluded that although aqueous extract and methanolic extract of stem bark provided significant effect as analgesic agent they haven’t provided better effect or, same effect as compared crude resin extract. Keywords: Guggulu resin, Guggulu stems bark, analgesic activity, Methanolic extract.
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Malviya Reeta et al, 2015/ Comparative evaluation of analgesic activity of Oleogumresin
International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (5) 01- 09 1
RESEARCH ARTICLE
International Research Journal of Pharmaceutical and Biosciences
Pri -ISSN: 2394 - 5826 http://www.irjpbs.com e-ISSN: 2394 - 5834
Comparative Evaluation of Analgesic Activity of Oleogumresin and Stem bark extract of Guggulu in Albino rats.
*1Dept. of Dravya Guna, Mahatma Jyotiba Fule Ayurvedic college, chomu, Jaipur, Rajasthan. 2Dept. of P.G. Studies in Dravya Guna, National Institute of Ayurveda, Jaipur, Rajasthan. 3Dept. of P.G. Studies in Dravya Guna, National Institute of Ayurveda, Jaipur, Rajasthan. 4Dept. of P.G. Studies in Dravya Guna. National Institute of Ayurveda, Jaipur, Rajasthan. 5Dept. of P.G. Studies in Dravya Guna. National Institute of Ayurveda, Jaipur, Rajasthan.
Background: Guggulu (commiphora wightii Bhandari) is a very important plant widely used for its antihyperlipidaemic, analgesic and anti- inflammatory actions by both Ayurvedic contemprory medicinal practionars. The Oleo-gumresin is a very widely used raw material of this medicinal plant and it is commonly used in Vata roga and Medo roga in Ayurveda. Objective: The current research paper provides a detailed report of comparative study the Analgesic activity of Guggulu resin & its stem bark extract in albino rats. Methods: An artery clip is applied to the root of the tail of mice and the reaction time is noted. Male mice with a weight between 18 and 25 g are used. The Control Group, Standard Group and Test Group consist of 6 mice in each. The test compounds are administered orally to fasted animals. The drug is administered 15, 30 or 60 min prior testing. An artery clip is applied to the root of the tail (approximately 1 cm from the body) to induce pain. The animal quickly responds to these noxious stimuli by biting the clip or the tail near the location of the clip. A stopwatch in 1/10 seconds increments measures the time between stimulation onset and response. A cut – off time will determine by taking the average reaction time plus 3 times the standard deviation of combined latencies of the control mice at all times periods. Any reaction time of the best animals which greater. Then the cut-off time is called a positive response indicative of analgesic activity. The length of time until response indicates the period of greatest activity after dosing. An ED50 value is calculated at the peak time of drug activity. Results: All three test sample provided significant effect as Analgesic agent. Conclusion: It can be concluded that although aqueous extract and methanolic extract of stem bark provided significant effect as analgesic agent they haven’t provided better effect or, same effect as compared crude resin extract.
Malviya Reeta et al, 2015/ Comparative evaluation of analgesic activity of Oleogumresin
International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (5) 01 - 09 2
INTRODUCTION Guggulu (commiphora wightii Bhandari) is a very important plant widely used for its antihyperlipidaemic, analgesic and anti- inflammatory actions by both Ayurvedic contemprory medicinal practionars. The Oleo-gumresin is a very widely used raw material of this medicinal plant and it is commonly used in Vata roga and Medo roga in Ayurveda. Unfortunately the plant Commiphora wightii has become endangered because of its slow
growing nature, poor seed setting, and lack of cultivation, poor seed germination rate and
excessive and unscientific tapping for its gum resin by the pharmaceutical industries and
religious prophets. This plant is incorporated in Data Deficient category of IUCN’s Red Data
list. The plant Commiphora wightii has become endangered because of its slow growing
nature, poor seed setting, and lack of cultivation, poor seed germination rate and excessive
and unscientific tapping for its gum resin by the pharmaceutical industries and religious
purposes. In case of C. wightii, stem cuttings offer several advantages over seeds. They save
time and labor, and produce genetically superior and uniform planting materials from
superior parentstocks. Stem cuttings are also inexpensive and easier to practice than other
vegetative -propagation methods, such as tissue culture. In addition, stem cuttings can
continuously supply planting stocks throughout the year for reforestation activities.
Pain is a subjective symptom and a major health problem throughout the globe now days as
it leaves major negative effects of physical and psychological health of the people. The gap
between the sophisticated knowledge of pain and its treatment and the effective application
of that knowledge is large and widening. Both acute and chronic pain is often poorly
managed for a wide variety of reasons.
In India 30% of people, women and elderly in particular and other adults in general are
affected with chronic pain. About 20-25% of this population suffer pain from
musculoskeletal and joint disorders. The back pain accounts for another 25-30% people.
Headache and migraine accounts for about 5% among the cases of persistent pain. In only 1-
2 % of adults with chronic pain result from cancer. Trigeminal neuralgia, post hepatic
neuralgia and other neuropathic pain syndromes constitute another 5-7% of patients
suffering from chronic pain. Now interventional pain management procedures like trigger
point injections, percutaneous disectomy and nerve blocks etc. however with no good
results. Hence an attempt has been made to find out simple and safe remedies from
Ayurveda.
Mode of Action
Guggulu can counteract upon Shita and reduce pain sansationsa by having direct effect on
Vata. Hence Guggulu have been described as best Vathara drug of Ayurveda. Vagbhat has
mentioned Guggulu as “xqXxqyq esnks·fuykgjk.kke~”A in Agrya Prakarana. And it pacifies
the Vata by its Vatashamak action releiving pain.
Malviya Reeta et al, 2015/ Comparative evaluation of analgesic activity of Oleogumresin
International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (5) 01 - 09 3
A dominant Analgesic activity of Commiphora wightii (guggul) plant, earlier known as
Commiphora mukul (family-Burseraceae) add impetus to this research approach. Oleogum
resin, known as guggul or gum guggul, is obtained from C. mukul found in India, Bangladesh
and Pakistan (Dev, 1987). One of the most revered plant Guggul, Commiphora wightii, has
been used in Ayurvedic medicine to treat a variety of diseases since several thousand years
(Satyavati, 1988). It is used extensively for the treatment of different ailments including