Sanitization Sanitization Common Sanitization Practices and Common Sanitization Practices and Procedures in University Laboratory Animal Resources Animal Resources Justin Lahmers Justin Lahmers August 25, 2010 ULAR Continuing Education & Professional Development Class
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SanitizationSanitization
Common Sanitization Practices andCommon Sanitization Practices and Procedures in University Laboratory
Animal ResourcesAnimal Resources
Justin LahmersJustin LahmersAugust 25, 2010ULAR Continuing Education & Professional Development Class
• “Keeping oneself and the work• Keeping oneself and the work area neat and orderly is an
ti l t f litessential part of a quality husbandry program. It conveys an attitude of professionalism and enhances sanitation and hygiene programs.”
– Page 72; ALAT Training Manual (1998 edition)
What Are We Going To CoverCover
• Definitions• Definitions
• Products• Products
• Application• Application
• Monitoring• Monitoring
DefinitionsDefinitions
• CleaningCleaning– The complete removal of all visible soil
from the surfacefrom the surface
DefinitionsDefinitions
• SanitizationSanitization– The process by which the number of
bacteria and other organisms living onbacteria and other organisms living on inanimate objects is reduced to an acceptable standard. Sanitization does not necessarily totally eliminate all microorganisms, but is aimed at
d i t t l b f ireducing total numbers of organisms
DefinitionsDefinitions
• SanitizationSanitization– The process involves the removal of
dirt hair saliva blood feces and urinedirt, hair, saliva, blood, feces and urine
– This is followed by washing with– This is followed by washing with detergents and rinsing with a chemical sanitizer or water at 180ºF (82.2ºC)( )
DefinitionsDefinitions
• DisinfectionDisinfection– This process is a more intense form of
sanitation and is designed to reduce tosanitation, and is designed to reduce to a harmless level the number of a specific type of organism, specifically y g ypathogenic organisms (not necessarily spores), on an object
Definitions
• Sterilization
Definitions
Sterilization– In this context
refers to therefers to the process of rendering an gobject totally free of all living
iorganisms
What Do We Sanitize?
• Cages • Sinks
What Do We Sanitize?
Cages• Feeders• Wire bar lids
Sinks• Runs (kennels)• Water bowlsWire bar lids
• Water bottles• Racks
Water bowls• Recoil hoses• Feed bins• Racks
• Carts• Floors walls
• Feed bins• Hoods• Enrichments• Floors, walls,
ceilings (rooms)• Water lines
• Enrichments devices/toys
• Supply and• Water lines• Cage lids
• Supply and exhaust hoses
What Products Do We Use?What Products Do We Use?• STERIS Process NPD• Chlorine Bleach• STERIS Cage-Klenz - (100 150 200)• STERIS Cage-Klenz - (100, 150, 200)• STERIS Foam 140 and 240
STERIS S Kl• STERIS Spor-Klenz• STERIS ALCARE (foam hand rub)• STERIS Kindest Kare (hand and bath
soaps)
What Products Do We Use?What Products Do We Use?
No more Joy in ULAR!
What Products Do We Use?
• STERIS GP Cleaner
What Products Do We Use?
• STERIS GP Cleaner– General cleaning
detergentdetergent
– Ideal for general gcleaning prior to use of a sanitizing
l tisolution
– Multiple dilution rates– Multiple dilution rates depending on use
Considerations Regarding P d t S l tiProduct Selection
• Label claimsLabel claims • Spectrum of activity
Eff ti i h d t• Effectiveness in hard water • Stability of the pH • Use dilution • Contact timeContact time • Temperature
Application MethodsApplication Methods
• Mops (sponge and cotton) andMops (sponge and cotton) and squeegees
– Cloth, sponge, mop or course sprayContact Time• Contact Time – Allow treated surfaces to remain wet for
ten minutesten minutes
Process NPD®
Environmental DisinfectantEnvironmental Disinfectant• Very ImportantVery Important
– Surfaces must be thoroughly rinsed with water after ten minutes of contact timewater after ten minutes of contact time or dry before animals come into contact with treated surface
– Should not be used for feeders and water bowls
Chlorine to Sanitize BowlsChlorine to Sanitize Bowls
• Sanitize feed and water bowls withSanitize feed and water bowls with chlorine bleach when a cage washer is not availableis not available
P dil ti• Proper dilution– Measure 12 ounces or 1 and ½ cups
bl h ll f tbleach per gallon of water
Chlorine BleachChlorine Bleach
• Used in auto water systems to• Used in auto water systems, to hyper chlorinate water lines on
k d t iti f d dracks and to sanitize feed and water bowls when a cage washer is not available
• Does not contain dirt loosening d t tdetergents
Hyper ChlorinationHyper Chlorination
Hyper ChlorinationHyper Chlorination
Water System ChlorinationWater System Chlorination
• To reduce pathogenicTo reduce pathogenic microorganisms in the water supply it can be treated with chlorinecan be treated with chlorine
STERIS Foam ProductsSTERIS Foam Products
• When used with suitable foamingWhen used with suitable foaming spray equipment, these products develop clinging foam to increasedevelop clinging foam to increase cleaning contact time and reduce overall laboroverall labor
against hard water scale d i lorganic soils and urine scale
Cage WashingCage Washing
• Uses a combination of water andUses a combination of water and cage cleaning chemistries to sanitize cages racks and accessoriescages, racks and accessories
Cage WashingCage Washing
• Programmable cycles consisting ofProgrammable cycles consisting of prewash, wash, rinse and final 180ºF (82 2ºC) rinse(82.2 C) rinse
• A liquid phosphate-free detergentA liquid phosphate free detergent system for use in high pressure spray animal cage washing equipmentanimal cage washing equipment
• Removes urine scale, organic soils, and metal oxidesand metal oxides
• An acid cleaner for removal of a wideAn acid cleaner for removal of a wide spectrum of soils. It contains wetting and penetrating agentsand penetrating agents
• Removes hard water scale, urine scale and metal oxidescale and metal oxide
• Red Label is Acid
Moist Heat SterilizationMoist Heat Sterilization
AutoclaveAutoclave• Definition of autoclave from
Dictionary comDictionary.com– (Noun) an apparatus for sterilizing
objects or for cooking by means ofobjects or for cooking by means of steam under pressure
Moist Heat SterilizationMoist Heat Sterilization• Steam and high pressure combine to g p
create superheated steam in excess of 212ºF (100ºC)( )
• Minimum times for a cycle are usually• Minimum times for a cycle are usually 15 minutes at 121ºC (250ºF) at 15 psi
• Flash cycles of 5 minutes at 132.2ºC (270ºF)
Moist Heat SterilizationMoist Heat Sterilization
• Liquid cycles take longer for theLiquid cycles take longer for the water to heat to the appropriate temperature and they slow exhaust totemperature and they slow exhaust to minimize evaporation loss