Columnsand Consumables for GCand GC/MS - … · TR-SimDist 100% Dimethyl Polysiloxane 400°C for ﬁlms

Analyze • Detect • Measure • Control™

A trace of gold for your GC or GC/MS

TRACE™ Capillary GC ColumnsThe new line of TRACE capillary GC columns from Thermo covers awide polarity range for all application areas, ideal for all GC andGC/MS users. High usable temperatures and low bleed are standard,increasing analytical sensitivity, reliability and column lifetime.

SyringesAccurate and robust, the Thermo syringes suit not only Thermo GCinstruments and autosamplers, but other leading manufacturers’instruments as well. They incorporate design features that makethem easy-to-use, enhancing the repeatability of injection and thereliability of results.

GC Injection Port LinersThermo injection port liners are precision manufactured and highlydeactivated, available for all leading GC instrument manufacturers.The innovative FocusLiner™ will improve reproducibility and extendyour GC column lifetime.

Septa and FerrulesThermo offers a full range of septa and ferrules to improve the per-formance of your GC instrument. Septa are available in a variety ofmaterials and diameters to suit all applications. High quality graphiteand Vespel® ferrules, as well as SilTite™ metal ferrules optimized foreasy use with GC/MS interfaces, are supplied in all dimensions.

Columns and Consumablesfor GC and GC/MS

c h r o m a t o g r a p h y

TRACE Capillary GC Columns

PHASE PHASE TYPE MAXIMUM TEMP USP CODE

TR-1 100% Dimethyl Polysiloxane 340°C/360°C for films 1.0 µm; 300°C/320°C for 3.0 µm films; G1, G2, G38280°C/300°C for films >3.0 µm

TR-1MS 100% Dimethyl Polysiloxane 370°C/380°C G1, G2, G38TR-5 5% Phenyl Methylpolysiloxane 340°C/350°C for films 1.5 µm; 280°C/300°C for films >1.5 µm G27, G36, G41TR-5MS 5% Phenyl Polysilphenylene-siloxane 360°C/370°C for films less than 1.5 µm; 350°C/360°C for films > 1.5 µm G27, G36, G41TR-35MS 35% Phenyl Polysilphenylene-siloxane 360°C/370°C G42TR-1701 14% Cyanopropylphenyl Polysiloxane 280/300°C G46TR-50MS 50% Phenyl Polysilphenylene-siloxane 360°C/370°C G3, G17TR-WAX Polyethylene glycol (PEG) 260°C/280°C for films <1.0 µm; 240°C/260°C for 1.0 µm films G14, G15, G16, G20, G39TR-WaxMS Polyethylene Glycol 280/300°C G14, G15, G16, G20, G39TR-SimDist 100% Dimethyl Polysiloxane 400°C for films <1.0 µm; 370°C for 2.65 µm films N/ATR-FAME 70% Cyanopropyl Polysilphenylene-siloxane 250°C/260°C N/ATR-V1 Cyanopropylphenyl Polysiloxane 280°C/300°C G43

TRACE capillary GC columns from Thermo Electron Corporation areavailable in a wide range of polarities and dimensions from the

general purpose non-polar and polarcolumns to specialist phases optimized forspecific analyses. All the columns are char-acterized by stability at high temperatures,exhibit low bleed and long lifetimes. Most ofthe columns are available in MS-compatibleformats with extremely low bleed levels toreduce source contamination. TRACE GCcolumns offer high quality and performance,with guaranteed reproducibility from column-to-column and year-to-year.

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Recommended Velocities & Flow Ratesfor Capillary Column

CARRIER GAS 0.25 mm ID 0.32 mm ID 0.53 mm IDmL/min cm/min mL/min cm/min mL/min cm/min

He 1 35 1.7 35 6 35H2 1.6 50 2.6 50 7.5 50N2 0.4 14 0.5 11 0.9 7

Recommended Detector Gas Flow Rates

DETECTOR AIR (mL/min) H2 (mL/min) MAKE UP (mL/min)

ECD – – 35-40FID 350 35 30NPD 60 2.5 15

Unretained Compounds

DETECTOR ANALYTE

FID MethaneECD Methylene ChlorideNPD AcetonitrileTCD,MS Methane, ButanePID, ELCD Vinyl Chloride

TRACE TR-1

The TRACE TR-1 from Thermo ElectronCorporation is a general purpose non-polarcolumn suitable for a wide range of com-pounds including hydrocarbons, esters, pesticides, drugs and amines. An industrystandard non-polar column suitable for a verywide range of applications, the TR-1 is anextremely low bleed phase with a maximumoperating temperature of 360°C. The TR-1 issuitable for a range of detectors includingMS. The column is solvent washable.

Similar to:DB-1, DB-Petro, BP1, HP-1, HP-1MS, Rtx-1,Ultra-1, SPB-1, SPB-1 Sulfur, Petrocol DH,CP-Sil 5CB, RSL-150, RSL-160, ZB-1, CB-1,OV-1, PE-1, 007-1(MS), SP-2100, SE-30, RH-1, CC-1, CP-Sil 5CB MS, VF-1ms

ApplicationsThe selected sample application demonstratesthe universal application of the TR-1 column.The application shows excellent resolutionof a group of phthalate plasticizers. With anmaximum temperature limit of 360°C, it iseasy to bake out and recondition the columnin the event of column contamination.

Figure 1: Analysis of phthalate esters on TRACE TR-1 column

Column: TR-1, 15 m x 0.25 mm x 0.25 µmPart No.: 260A130PPhase: TR-1, 0.25 µm filmInitial Temp: 200°C, 0 minRate: 20°C/minFinal Temp: 280°C, 2 minDetector: FIDSensitivity: 128 x 10-12 AFSInjection Mode: Split

Components1. Dimethyl Phthalate2. Diethyl Phthalate3. Di-iso-Propyl Phthalat4. Di-n-Propyl Phthalate5. Di-iso-Butyl Phthalate6. Di-n-Butyl Phthalate7. Di-n-Pentyl Phthalate8. Dihexyl Phthalate9. Diheptyl Phthalate10. Dioctyl Phthalate

Figure 2: Pyrolysis of high density polyethylene on TRACE TR-1 column

Column: TR-1, 30 m x 0.32 mm x 0.5 µm

Part No.: 260A224PPhase: TR-1, 0.5 µmInitial Temp: 50°CRate: 10°C/minFinal Temp: 320°C, 10 minDetector: FIDPyrolysis Temp: 800°CCarrier Gas: H2, 5 psi

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TRACE Capillary G

C Columns

TRACE TR-1MSAn industry standard non-polar column suitable for a wide range of applications.An extremely low bleed phase with a maximum operating temperature of 380°C, theTR-1MS from Thermo Electron Corporationis ideal for all GC/MS applications. The column is particularly suited for applicationswhich require a high temperature phasethat is also inert.

Similar to:Suitable Upgrade from DB-1, DB-Petro, BP1,HP-1, HP-1MS, Rtx-1, Ultra-1, SPB-1, SPB-1Sulfur, Petrocol DH, CP-Sil 5CB, RSL-150,RSL-160, ZB-1, CB-1, OV-1, PE-1, 007-1(MS),SP-2100, SE-30, RH-1, CC-1, CP-Sil 5CB MS,VF-1ms

ApplicationsThe TRACE TR-1MS is ideal for environmentalapplications such as the analysis of chlori-nated and nitroaromatic compounds. Theinertness and high temperature capability ofthe column are features which are well suitedto LC/MS environmental analyses.

Figure 3: Analysis of chlorine, phosphorus and nitrogen-based pesticides

Column: TR-1MS, 30 m x 0.25 mm x 0.25 µmPart No.: 260B142P Initial Temp: 50°C,1 minRate1: 4°C/minFinal Temp: 280°C, 1.5 minDetector: Mass SpecCarrier Gas: Helium, 33 cm/secInjection Mode: Splitless, 0.5 min

Figure 4: Analysis of chlorinated and nitroaromatic compounds on a TRACE TR-1MS column

Components1. Phenol2. o-Chlorophenol3. p-Dichlorobenzene4. Nitrobenzene5. o-Nitrophenol6. 2,4-Xylenol7. 2,4-Dichlorophenol8. 4-Chloro-3-methylphenol9. 2,4,6-Trichlorophenol

10. 2,6-Dinitrotoluene11. 2,4-Dinitrophenol12. 2,4-Dinitrotoluene13. 4-Nitrophenol14. 4,6-Dinitro-o-cresol15. 4-Chlorophenyl phenyl ether16. 4-Bromophenyl phenyl ether17. Hexachlorobenzene18. Pentachlorophenol

Column: TR-1MS, 30 m x 0.25 mm x 0.25 µmPart Number: 260B142PSample: 200 ppm in dichloromethaneInitial Temp: 40°C, 1 min.Rate 1: 10°C/min to 300°C,Final Temp: 300°C, 2 min.Detector Type: MSDCarrier Gas: He, 25.7 psiCarrier Gas Flow: 1.8 mL/min.Constant Flow: OnAverage Linear Velocity: 35 cm/sec at 40°CInjection Mode: SplitSplit Ratio: 100:1Injection Volume: 0.5 µLInjection Temperature: 250°CLiner Type: 4 mm ID Single Taper LinerLiner Part Number: 453A1345Full Scan/SIM: Full scan 45-450

Components1. DDVP2. Etridiazole3. Chloroneb4. Molinate5. BPMC6. Benfluralin7. Pencycurone8. Simazin9. Diazinon-ox

10. Propyzamide11. Diazinon12. TPN13. IBP14. ECP15. MBPMC16. Bromobutide17. MEP-ox18. Tolclophos-Methyl

19. Simetryn20. MEP21. Esprocarb22. Malathion23. Probenazole24. Chloropyrifos25. Thiobancarb26. Fthalide27. Pendimetaline28. Isofenfos

29. Methyl-Dymron30. Captan31. Butamifos32. Napropamide33. Flutolurin34. Prethirachlor35. Isoxathon-ox36. Isoprothiolate37. Tricyclazole38. Buprofezine

39. Isoxathion40. Mepron:141. Chloronitrofen42. EDDP43. EPN-ox44. Pyridaphenthion45. Iprodione46. EPN47. Bifenox48. Mefenacet

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TRACE TR-5The TRACE TR-5 5% phenyl column is anon-polar, low bleed column with a slightincrease in polarity compared with the100% methyl columns. The TR-5 column is a universal column used in a wide range ofapplications. This phase is extremely lowbleed with a maximum operating tempera-ture of 350°C.

Similar to:DB-5, BP5, Rtx-5, HP-5, Ultra-2, PTE-5,SPB5, MDN-5, CP-Sil 8CB, SPB-5, AT-5,MDN-5, ZB-5, 007-2(MPS-5), SE-52, SE-54

ApplicationsThe TRACE TR-5 is a general purpose column,ideal for a wide variety of applicationsincluding alcohols, free fatty acids, aromatics,flavors and low polarity pesticides. The universal use of the TR-5 column is illustratedby the separation of substituted aromatics.The chromatogram shows excellent peakshape of all components and separation isbased on boiling points.

Figure 5: Analysis of substituted aromatics

Column: TR-5, 30 m x 0.25 mmx 0.25 µm

Part No.: 260E142PInitial Temp: 40°C, 4 minRate 1: 2°C/minTemp 2: 60°CRate 2: 5°C/minFinal Temp: 80°C, 6 minDetector: FIDInjection Mode: Split

Figure 6: Analysis of chlorinated hydrocarbons on a TRACE TR-5 column

Column: TR-5, 15 m x 0.53 mm x 1.0 µmPart No. 260E286PInitial Temp: 85°C, 0 minRate: 10°C/minFinal Temp: 195°C, 0 minDetector: FIDInjection Mode: Split

1. Chlorobenzene2. 1,2-Dichlorobenzene3. 1,3-Dichlorobenzene4. Hexachloroethane5. Hexachlorobutadiene6. Hexachlorocyclopentadiene7. 2-Chloronaphthalene8. Hexachlorobenzene

Components1. Benzene2. Toluene3. Chlorobenzene4. Ethylbenzene5. m,p-Xylene6. o-Xylene7. Styrene8. iso-Propylbenzene9. n-Propylbenzene10. 1,3,5-Trimethyl-benzene11. 1,2,4-Trimethyl-benzene12. m-Dichlorobenzene13. sec-Butylbenzene,

p-Dichlorobenzene14. o-Dichlorobenzene15. Butyl-benzene16. 1,2,4-Trichloro-benzene17. Napthalene18. Hexachlorobutadiene

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TRACE Capillary G

C Columns

TRACE TR-5MSThe TRACE TR-5MS column from ThermoElectron Corporation is a silphenylene-basedphase ideal for all GC/MS applications. A low bleed column is especially importantfor MS as contamination of source compo-nents will quickly lead to a degradation ofresults and costly instrument downtime. TheTR-5MS column is a low bleed column witha slight increase in polarity compared withthe 100% methyl TR-1 columns. If theGC/MS is exposed to many varied methodsthen this is the preferred column of choicefor the instrument. This phase is extremelylow bleed with a maximum operatingtemperature of 370°C.

Similar to:DB-5, DB-5ms, DB-5.625 XTI-5, BPX5, Rtx-5ms, Rtx- 5, SilMS, AT-5, AT-5MS, 007-5MS, SPB-5, CP-Sil 8CB, Ultra-2, HP-5,HP-5MS, HP5-TA, SPB-5, MDN-5S, VF-5ms,RSL-200, CB-5, OV-5, PE-5, 007-2(MP-5), SE-52, SE-54, PTE-5, CC-5, RH-5ms, ZB-5

Figure 8: Organochlorine pesticides

Column: TR-5MS, 30 m x 0.25 mm x 0.5 µmPart No.: 260F223PInitial Temp: 40°C, 1 minRate 1: 30°C/minTemp 2: 190°C, hold 3 minRate 2: 10°C/minFinal Temp: 300°C, hold 5 minDetector: FIDCarrier Gas: HeliumInjection Mode: Splitless

Components1. 2,4,5,6-tetrachloro-meta-xylene2. α-BHC3. β-BHC4. γ-BHC5. δ-BHC6. Heptachlor7. Aldrin8. Heptachlorepoxy9. gamma-chlordane10. alpha-chlordane11. Endosulfan A12. p,p’-DDE13. Dieldrin14. Endrin15. p,p’-DDD16. Endosulfan B17. Endrin Aldehyde18. p,p’-DDT19. Endosulfan Sulfate20. Methoxychlor21. Endrin Ketone22. Decachlorobiphenyl

Figure 7: Basic drug screen on a TR-5MS (10-20 ng/component)

Column: TR-5MS, 30 m x 0.53 mm x 1.0 µm

Part No.: 260F298P10-20ng/component

Initial Temp: 120°CRate: 10°C/minFinal Temp: 310°CDetector: FIDInjector: Split, 240°CCarrier Gas: Hydrogen, 2 psi

Notes: The low bleed nature of the TR-5MS allows trace analysis to be performed

Components1. Methamphetamine2. Phendimetrazine3. Phencyclidine4. Mepivocaine5. Methaqualone

6. Amitriptyline7. Codeine8. Diazepam9. Fentanyl

ApplicationsThe TR-5MS column is a universal columnused in a wide range of applications fromenvironmental semi-volatile methodsthrough to food and fragrance applications.Organochlorine pesticides analysis isextremely demanding and requires a columnwhich is inert and low bleed. The TR-5MScolumn is ideal for this analysis as shown.

The column is very inert (no active sites)thus preventing breakdown of labile compounds such as DDT and Endrin. Theinertness of the column also results in minimal peak tailing. This together withhigh efficiency results in narrow peaks andexcellent separation based on boiling points.

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TRACE TR-35MSThe TRACE TR-35MS is a silphenylene-basedphase ideal for all GC/MS applications. Thecolumn is particularly suited for applicationswhich require a high temperature phase butmore polarity than a 5% phenyl column. Alow bleed column is especially important for GC/MS as contamination of source components will quickly lead to a degradationof results and costly instrument downtime.This phase is extremely low bleed with amaximum operating temperature of 370°C.

Similar to:DB-35, DB-35ms, HP-35, HP-35MS, MDN-35,RTX-35, SPB-35, BPX35

ApplicationsThe TR-35MS column is a universal columnused in a wide range of applications fromenvironmental semivolatile methods to foodand fragrance applications. The analysis ofamines is extremely demanding and requiresa column which is inert and low bleed. TheTR-35MS column from Thermo is ideal forthis analysis and this application demon-strates the excellent features of the column.The inertness of the column also results inminimal peak tailing. This together withhigh efficiency results in narrow peaks andexcellent separation.

Figure 9: Analysis of aromatic amines from diazo dyes

Part No.: 260C142PAzo Dyes standard: 10 ppm solution in DCMInitial Temp: 50°C, 2 min.Rate 1: 15°C to 240°CRate 2: 10°C to 280°CFinal Temp: 280°C, 25 min.Detector Type: Mass SpecCarrier Gas: Helium, 7.1 psiCarrier Gas Flow: 1.0 mL/min.Constant Flow: OnAverage Linear Velocity: 36 cm/sec at 50°CInjection Mode: SplitlessSplitless Time: 1.0 min.Splitflow: 60 mL/min.Injection Volume: 1 µLInjection Temperature: 250°CAutosampler: NoLiner Type: 4 mm ID Double Taper Liner

Components1. Indolin2. o-Toluidine3. 2,4-Diaminoanisole4. p-Chloroaniline5. p-Cresidine6. 2,4,6-Trimethylaniline7. 4-Chlorotoluidine8. Unknown9. 2-Naphthylamine10. Unknown

Figure 10: Analysis of a phenol mixture on TR-35MS

Column: TR-35MS, 30 m x 0.25 mm x 0.25 µm

Part Number: 260C142PSample: 200 ppm in methanolInitial Temp: 80°C, 1 min.Rate 1: 10°C/min to 300°C,Final Temp: 300°C, 5 min.Detector Type: Mass SpectrometerCarrier Gas: Helium, 29.2 psiCarrier Gas Flow: 1.7 mL/minAverage Linear Velocity: 35 cm/sec at 80°CInjection Mode: SplitSplit Ratio: 100:1Injection Volume: 1 µLInjection Temperature: 250°CLiner Type: 4 mm ID Single Taper LinerLiner Part Number: 453A1345Full Scan / SIM: Full scan 45-450

Components1. Phenol2. 2-Chlorophenol3. o-Cresol4. p-Cresol5. m-Cresol6. 2,5-Dimethylphenol7. 2,4-Dimethylphenol8. 2-Nitrophenol9. p-Ethylphenol

10. 3,5-Dimethylphenol11. 2,4-Dichlorophenol12. 2,3-Dimethylphenol13. Isopropylphenol14. 4-Chloro-3-methylphenol15. 2,4,6-Trichlorophenol16. 4-Nitrophenol17. 4,6-Dinitrocresol

11. 4-Aminodiphenyl12. 4,4'-Ox ydianiline13. 4,4'-Diaminodiphenylmethane14. Benzidine15. 3,3'-Dimethyl-4,4’-diaminodiphenylm ethane16. 3,3'-dimethylbenzidine17. 4,4'-Thiodianiline18. 3,3'-Dichlorobenzidine19. 4,4'-Methylene-bis(2-chloroaniline)20. 3,3'-Dimethoxybenzidine

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TRACE TR-1701The TRACE TR-1701 column is a popularmid-polarity column that sits between theTR-50MS and the TR-35MS in polarity. It ischaracterized by low bleed and high temper-ature stability compared with other similarcolumns. The cyano and phenyl groupsincrease polarity of the column while offeringalternative selectivity. This makes the TR-1701column especially suited for confirmationalanalysis when used in conjunction with acolumn of a different polarity, such as the

TRACE TR-5MS. The TR-1701 is ideal for pesticide and other environmental applications.

Similar to:DB-1701, Rtx-1701, HP-1701, BP10, OV-1701,007-1701, CP-Sil 19 CB

ApplicationsIdeal for many applications, the TRACE TR-1701 is particularly useful for the analysisof pesticides, PCBs, PAHs, organic acids,

drugs and steroids. The different selectivityof the TR-1701 column is particularly usefulfor the analysis of pesticides and otherapplications where a different elution order isrequired for confirmation purposes as shownin Figure 11. The analysis of organochlorinepesticides on the TR-1701 compared to theTR-5MS highlights the different retentioncharacteristics that can be achieved. Thecomparison of the different retention timeson the two columns greatly improves thereliability of analyte identification.

TRACE Capillary G

C Columns

Figure 11. Analysis and confirmation of organochlorine pesticides

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Injector Temp: 240°CInjection Volume: 1 µLInjection Type: Splitless, 0.5 minPurge On (Split) Vent: 60 mL/minLiner Type: Single taperCarrier Gas: HeliumConstant Flow: OnPressure: 14.1 psi

Column Flow: 1.3 mL/minLinear Velocity: 30 cm/sec at 40°CInitial Temp: 40°C, 1 minRate 1: 30°C/minFinal Temp 1: 190°C, 3 minRate 2: 10°C/minFinal Temp 2: 300°C, 5 minDetector: FID, 310°C

1. 2,4,5,6-Tetrachloro-m-xylene (IS)2. α-BHC3. β-BHC4. γ-BHC5. δ-BHC6. Heptachlor7. Aldrin8. Heptachlorepoxide9. γ-Chlordane10. α-Chlordane11. Endosulfan A12. p,p’-DDE13. Dieldrin14. Endrin15. p,p’-DDD16. Endosulfan B17. Endrin Aldehyde18. p,p’-DDT19. Endosulfan Sulphate20. Methoxychlor21. Endrin Ketone22. Decachlorobiphenyl (IS)

TRACE TR-50MSThe TR-50MS from Thermo ElectronCorporation is a Silphenylene-based phaseideal for all GC/MS applications. A lowbleed column is especially important forGC/MS as contamination of source compo-nents will quickly lead to a degradation ofresults and costly instrument downtime.This phase is extremely low bleed with amaximum operating temperature of 370°C.

Similar to:OV-17, SP-2250, DB-17, DB-17ms, DB-17ht,BPX50, Rtx-50, SPB-50, HP-50+, HP-17,AT50, RSL-300, PE-17, CC-17, 007-17(MPS-50),SPB-17, ZB-50

ApplicationsThe TR-50MS column is particularly suitedfor applications which require a high temperature phase but more polarity than a 5% phenyl column. The TR-50MS can beused in combination with a 5% phenyl column as a confirmation column. The sampleapplication of a mixture of pesticidesdemonstrates the excellent separation of amedium polarity compounds on a mid-polaritycolumn. The inertness of the column alsoresults in minimal peak tailing. This togetherwith high efficiency results in narrow peaksand excellent separation.

Figure 13. Analysis of chlorine, phosphorus and nitrogen-based pesticides

Figure 12. Antidepressant and anticonvulsant drugs on a TRACE TR-50MS column

Column: TR-50MS, 30 m x 0.25 mm x 0.25 µmPart No.: 260R142PInitial Temp.: 50°C, 1 minRate 1: 4°C/minFinal Temp.: 280°C, 1.5 min

Detector: Mass SpecInjector Mode: Splitless, 0.50 minCarrier Gas: Helium, 33 cm/secInjection Volume: 0.5 µL

Components1. DDVP2. Etridiazole3. Chloroneb4. Molinate5. BPMC6. Benfluralin7. Pencycurone8. Simazin9. Diazinon-ox10. Propyzamide11. Diazinon12. TPN13. IBP14. ECP15. MBPMC16. Bromobutide17. MEP-ox18. Tolclophos-Methyl19. Simetryn20. MEP21. Esprocarb22. Malathion23. Probenazole24. Chloropyrifos

25. Thiobancarb26. Fthalide27. Pendimetaline28. Isofenfos29. Methyl-Dymron30. Captan31. Butamifos32. Napropamide33. Flutolurin34. Prethirachlor35. Isoxathon-ox36. Isoprothiolate37. Tricyclazole38. Buprofezine39. Isoxathion40. Mepron:141. Chloronitrofen42. EDDP43. EPN-ox44. Pyridaphenthion45. Iprodione46. EPN47. Bifenox48. Mefenacet

Column: TR-50MS, 30 m x 0.25 mm x 0.25 µmPart Number: 260R142PSample: 5-10 ppm in methanolInitial Temp: 150°C, 0.5 min.Rate 1: 10°C/min to 180°CRate 2: 1.5°C/min to 220°CRate 2: 30°C/min to 260°CFinal Temp: 260°C, 5 minDetector Type: FIDDetector Temp.: 320°CCarrier Gas: He, 25.7 psiCarrier Gas Flow: 1.8 mL/minConstant Flow: On

Average Linear Velocity: 35 cm/sec at 40°CInjection Mode: SplitlessSplitless Time: 0.5 minSplit Flow: 60 mL/minInjection Volume: 1 µLInjection Temperature: 250°CLiner Type: 4 mm ID Single Taper LinerLiner Part Number: 453A1345Full Scan / SIM: Full scan 45-450

Components1. Benzothiazole2. Selegeline3. Methsuximide4. Ketamine5. Unknown6. Brompheniramine7. Imipramine8. Mianserin9. Diothiepin10. Carbamazepine11. Prazepam12. Diltiazem13. Pholcodine

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TRACE TR-WAXThe TR-WAX from Thermo ElectronCorporation is a polyethylene glycol basedphase ideal for the analysis of all polarcompounds. The TR-WAX is a highly cross-linked and fully deactivated column resultingin excellent peak shape.

Similar to:DB-Wax, BP20, Rtx-Wax, Stabilwax,HP20M, HP-Wax, HP-INNOWax,Supelcowax-10, AT-Wax, Nukol, CPWax52CB, SUPEROX II, CB-WAX, Stabilwax,PE-CW, CCWAX, 007-CW, Carbowax, HP-Innowax, PE-WAX, ZBWAX

ApplicationThe column is particularly suited to esters,alcohols, ketones and glycols. The column isalso suitable for the separation of aromaticisomers such as the xylene isomers. Theanalysis of alcohols, especially short chainalcohols, usually results in some tailing on anon-polar column, but this is not observedon the TR-WAX.

TRACE Capillary G

C Columns

Figure 14: Analysis of 18 alcohols

Column: TR-WAX, 30 m x 0.25 mm x 0.25 µmPart No.: 260W142PInitial Temp: 45°C, 2 minRate: 3°C/minFinal Temp: 80°C, 0 minDetector: FIDInjection Mode: Split

Components1. Methanol2. Propan-2-ol3. Ethanol4. sec-Butan-1-ol5. n-Propanol+2-Methyl-3-Buten-2-ol6. d,l-3-Methyl-2-Butan-1-ol7. Pentan-3-ol8. d,l-2-Pentan-1-ol9. n-Butanol10. 2,4-Dimethyl, Pentan-3-ol11. Hexan-3-ol12. 2-Methyl Prop-2-en-1-ol13. Crotyl Alcohol (2-Buten-1-ol)14. Hexan-2-ol15. Pentan-1-ol16. 2-Methyl, Pentan-1-ol17. 2-Ethyl Butan-1-ol18. Hexan-1-ol

Figure 15: Blood alcohol analysis on a TR-WAX column

Column: TR-WAX, 30 m x 0.32 mm x 1.0 µmPart No.: 260W297PInitial Temp: Isothermal, 60°CDetector: FIDInjection Mode: Split

Notes: The TR-WAX column allows the use of aqueous solutions.

Components1. Acetone2. Ethyl Acetate3. Methanol4. iso-Propanol5. Ethanol6. n-Propanol

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TRACE TR-WaxMSThe TRACE TR-WaxMS capillary GC columnfrom Thermo Electron is a low bleed, highlystable polar GC column. Generally, as polarityincreases, the column bleed also increases.This typically means that high polaritycolumns have lower temperature limits thanlower polarity equivalents. This not only limits the range of compounds that can beanalyzed by polar columns including waxcolumns, but also results in significantlyhigher phase bleed and more instrumentcleaning, especially if using mass spectrom-etry. The TRACE TR-WaxMS column hasbeen designed to improve on both of theselimitations by using a proprietary bonding

technique that produces a column with significantly lower bleed levels than otherhigh polarity columns available with anextended Maximum Allowable OperatingTemperature of 280°C. This stability alsogives it a strong resistance to water, makingthe TR-WaxMS one of the most versatile,sensitive, stable, and long lifetime waxphase columns on the market. This columnis ideal for solvent, flavor, fragrance, alcohol, ester and aldehyde analysis.

Similar to:DB-Wax, Rtx-Wax, Stabilwax, HP20M, BP20,HP-Wax, HP-INNOWax, SUPELCOWAX 10,AT-Wax, Nukol, CP Wax 52CB, ZB-Wax

ApplicationsThe TRACE TR-WaxMS is ideally suited tothe analysis of aromatic hydrocarbons, foodadditives, essential oils, alcohols, esters,aldehydes and keytones. The low bleedcharacteristics of this column make it especially suitable for use with a massspectrometric detector, giving higher sensitivity,better library matches and less instrumentcleaning. Figure 16 shows the analysis ofylang ylang oil analyzed using a TR-WaxMScolumn. Even at the relatively high tempera-ture of 260°C, the bleed level still allowsexcellent detection and quantitation, evenusing a mass spectrometer.

Figure 16: Analysis of ylang ylang oil

1. p-Methyl anisole2. α-Copaene3. Linalool L4. β-Caryophyllene5. Methyl benzoate6. α-Humulene7. α-Amorphene8. Germacrene9. Benzyl acetate10. δ-Cadinene11. α-Farnesene12. Geranyl acetate13. trans-Cinamyl acetate14. Farnesyl acetate15. Farnesol16. Benzyl benzoate17. Benzyl salicylate

Column: TR-WaxMS, 30 m x 0.25 mm x 0.25 µmPart No. 260X142PSample: Ylang Ylang oil (neat)Injection Temp: 250°CInjection Mode: SplitSplit Ratio: 120:1Injection Volume: 0.1 µLInitial Temp: 40°C, 2 min.Rate 1: 3°C/min to 260°C,Final Temp: 260°C, 10 min.Carrier Gas: helium, 25.7 psiCarrier Gas Flow: 1.8 mL/min.Constant Flow: OnAverage LinearVelocity: 35 cm/sec at 40°CDetector Type: FID

11

TRACE TR-SimDistThe TR-SimDist column has been specificallydesigned for simulated distillation analysis.This analysis places a heavy demand on thecolumn due to the high oven temperaturesrequired to elute the higher molecularweight hydrocarbons. With a temperaturelimit of 400°C, the TR-SimDist meets theserequirements. The phase is strongly cross-linked and the stable bleed profile alsoallows for consistent background subtractionwhich is common practice.

Similar to:DB-HT Sim Dis, DB-2887, BPX1, Rtx-2887,HP-1, Petrocol 2887, Petrocol EX2887

ApplicationsThe example application shows the analysisof a hydrocarbon standard and demonstratesthe low bleed of this simulated distillationcolumn.

TRACE Capillary G

C Columns

Figure 17: Analysis of standard mix for ASTM D2887

Column: TR-SimDist, 10 m x 0.53 mm x 2.65 µmPart No.: 260S348PInitial Temp.: 40°CRate: 15°C/minFinal Temp.: 350°C, 10 minDetector Temp: 400°CCarrier Gas: Helium, 20 mL/minInitial Temp.: 80°CRate: 15°C/minFinal Temp.: 350°C, 10 min

Figure 18: Retention time hydrocarbon standard on TR-SimDist

Column: TR-SimDist, 10 m x 0.53 mm x 0.9 µmColumn Part No.: 260S250PInitial Temp: 40°CRate: 10°C/minFinal Temp: 390°C, 10 minDetector Temp: 400°CCarrier Gas: Helium, 20 mL/min

Initial Temp.: 80°CRate: 10°C/minFinal Temp.: 390°C,10 min

Note: No background subtraction was performed

12

TRACE TR-FAMEThe TR-FAME from Thermo ElectronCorporation is a cyanopropylphenyl basedphase specifically designed for the separa-tion of Fatty Acid Methy Esters (FAMEs).The unique selectivity of the TR-FAME column provides excellent separation ofcis/trans isomers and FAMEs with variousdegrees of unsaturation. The maximumallowable operating temperature of 260°Calso provides versatility for the analysis ofhigh molecular weight FAMEs

Similar to:DB-23, BPX70, Rtx-2330, SP-2330, CP-Sil 88, SP-2380, HP-23, VF-23ms, 007-23, AT-Silar,PE-23

ApplicationsThe sample chromatogram shows the reten-tion and excellent separation of many FAMEcompounds. Many FAMEs have very similarmass spectra and therefore identification ofunknowns can be difficult. It is important tonote also that the elution order of FAMEs isvery dependent on gas chromatographicconditions and even a small oven temperatureramp change can result in peak elutionorder swapping.

Figure 20: Analysis of 24 FAMEs

Column: TR-FAME, 30 m x 0.25 mm x 0.25 µmPart No.: 260M142PSample: 100 ppm in HexaneInitial Temp: 150°C, 0.5 min.Rate 1: 10°C/min to 180°C,Rate 2: 1.5°C/min to 220°CRate 3: 30°C/min to 260°CFinal Temp: 260°C, 5 min.Detector Type: FIDDetector Temp: 280°C

Carrier Gas: Helium, 20.7 psiCarrier Gas Flow: 1.3 mL/min.Average Linear Velocity: 35 cm/sec at 150°CInjection Mode: SplitSplit Ratio: 15:1Injection Volume: 1 µLInjection Temperature: 250°C

Components1. C12:02. C14:03. C14:1W54. C16:05. C16:1W76. C18:07. C18:1W98. C18:2W69. C18:3W610. C18:3W311. C20:012. C20:1W913. C20:2W6

14. C20:3W915. C20:3W616. C20:4W617. C22:018. C22:1W919. C20:5W320. Unknown21. C22:4W622. C24:023. C24:1W924. C22:5W325. C22:6W3

Figure 19: Analysis of 13 sugar component alditol acetate mixture on TR-FAME

Column: TR-FAME, 30 m x 0.25 mm x 0.25 µmPart No.: 260M142PInitial Temp.: 190°C, 1 minProgram Rate: 3°C/minFinal Temp: 260°C, 10 minCarrier Gas: He, 50kPaDetector: MS (Electron Impact

Ionization, Ionization Potential 70eV) Scan 100m/z to 350m/z in 0.3s.

Injection Mode: Split 50:1

Components1. Erythritol2. 2-Deoxy-ribitol3. Rhamnitol4. Fucitol5. Ribitol6. Arabinitol7. Xylitol8. 2-Deoxy-glucitol9. Allitol10. Mannitol11. Galacitol12. Glucitol13. Myo-inositol

13

TRACE TR-V1The TR-V1 from Thermo Electron Corporationis a cyanopropylphenyl based phase idealfor the analysis of volatile compounds. Theselectivity and film thickness ensures goodretention and separation of volatile com-pounds at low oven temperatures. The column does have a high maximum operatingtemperature of 300°C which provides versatility in the event of samples containingless volatile compounds. The column can beheated quickly to remove contaminants thusreducing cycle times and increasing samplethroughput.

Similar to:DB-624, BPX Volatiles, Rtx Volatiles, VOCOL56, OV-624, AT-624, HP-VOC, CP-Select624CB, 007-624, ZM-624

ApplicationsThe TR-V1 has been customized for the bestresolution from both the front and backends of chromatograms.

TRACE Capillary G

C Columns

Figure 21: Analysis of US EPA 502.2 mix

Components1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. 1,1-Dichloroethene 8. Dichloromethane 9. trans-1,2-Dichloroethene 10. 1,1-Dichloroethane 11. 2,2-Dichloropropane 12. cis-1,2-Dichloroethene 13. Bromochloromethane14. Chloroform 15. 1,1,1-Trichloroethane 16. 1,1-Dichloropropene 17. Carbon tetrachloride 18. Benzene 19. 1,2-Dichloroethane 20. Trichloroethene 21. 1,2-Dichloropropane 22. Dibromomethane 23. Bromodichloromethane 24. cis-1,3-Dichloropropene 25. Toluene 26. trans-1,3-Dichloropropene 27. 1,1,2-Trichloroethane 28. Tetrachloroethene 29. 1,3-Dichloropropane 30. Dibromochloromethane 31. 1,2-Dibromoethane32. Chlorobenzene 33. Ethylbenzene 34. 1,1,1,2-Tetrachloroethane 35. m-Xylene 36. p-Xylene 37. o-Xylene 38. Styrene 39. Bromoform40. Isopropylbenzene 41. Bromobenzene42. 1,1,2,2-Tetrachloroethane43. 1,2,3-Trichloropropane44. n-Propyl benzene45. 2-Chlorotoluene46. 1,3,5-Trimethylbenzene47. 4-Chlorotoluene48. tert-Butylbenzene49. 1,2,4-Trimethylbenzene50. sec-Butylbenzene51. 1,3-Dichlorobenzene52. p-Isopropyltoluene53. 1,2-Dichlorobenzene54. n-Butylbenzene55. 1,4-Dichlorobenzene56. 1,2-Dibromo-3-chloropropan57. 1,2,4-Trichlorobenzene58. Hexachlorobutadiene59. Naphthalene60. 1,2,3-Trichlorobenzene

Column: TR-V1, 30 m x 0.25 mm x 1.4 µmPart No.: 260V332PUSEPA 502.2 mix: 200 ppm in Methanol Injection Mode: SplitSplit Ratio: 50:1Injection Volume: 1 µLInitial Temp: 40°C, 1 min. Injection Temp: 250°CRate 1: 6°C/min to 210°C Rate 2: 15°/min to 240°C

Liner Type: 4 mm ID Single Taper LinerFinal Temp: 240°C, 5 min.Carrier Gas: Helium, 22.8 psi Carrier Gas Flow: 1.3 mL/min. Average Linear Velocity: 35 cm/sec at 40°CDetector Type: Mass Spectrometer Full scan 45-450

14

TRACE TR-1 An industry standard general purpose non-polar column suitablefor a very wide range of applications including hydrocarbons,esters, pesticides, drugs and amines.

ID (mm) FILM THICKNESS (µm) LENGTH (m) PART NO.

0.10 0.10 10 260A020P0.25 0.25 15 260A130P0.25 0.25 30 260A142P0.25 0.25 60 260A154P0.25 0.50 30 260A223P0.25 1.00 30 260A296P0.25 1.00 60 260A308P0.32 0.25 15 260A131P0.32 0.25 30 260A143P0.32 0.25 60 260A155P0.32 0.50 30 260A224P0.32 1.00 30 260A297P0.32 1.00 60 260A309P0.32 3.00 30 260A395P0.53 0.50 15 260A213P0.53 0.50 30 260A225P0.53 3.00 30 260A396P0.53 5.00 30 260A470P

TRACE TR-1MSAn extremely low bleed, inert phase particularly suited for GC/MSand any applications that require high temperatures.


0.25 0.25 30 260B142P0.25 0.25 60 260B154P0.32 0.25 30 260B143P0.32 0.25 60 260B155P

TRACE TR-5A universal column used in a wide range of applications such as alco-hols, free fatty acids, aromatics, flavors and low polarity pesticides.


0.32 0.25 7 260E113P0.25 0.25 15 260E130P0.25 0.25 30 260E142P0.25 0.25 60 260E154P0.25 0.50 30 260E223P0.25 1.00 30 260E296P0.25 1.00 60 260E308P0.32 0.25 15 260E131P0.32 0.25 30 260E143P0.32 0.25 60 260E155P0.32 0.50 30 260E224P0.32 1.00 30 260E297P0.32 1.00 60 260E309P0.53 0.50 30 260E225P0.53 1.00 15 260E286P0.53 1.00 30 260E298P0.53 1.50 15 260E334P0.53 1.50 30 260E336P0.53 5.00 30 260E470P

TRACE TR-5MSIdeal for all GC/MS applications, particularly if the column isrequired for many different methods.


0.10 0.10 10 260F020P0.15 0.25 25 260F134P0.25 0.25 15 260F130P0.25 0.25 30 260F142P0.25 0.25 60 260F154P0.25 0.50 30 260F223P0.25 1.00 15 260F284P0.25 1.00 30 260F296P0.25 1.00 60 260F308P0.32 0.25 15 260F131P0.32 0.25 30 260F143P0.32 0.25 60 260F155P0.32 0.50 30 260F224P0.32 1.00 15 260F285P0.32 1.00 30 260F297P0.32 1.00 60 260F309P0.53 0.50 15 260F213P0.53 0.50 30 260F225P0.53 1.00 30 260F298P0.53 1.50 30 260F336P0.53 3.00 15 260F384P0.53 3.00 30 260F396P

TRACE TR-35MSA mid-polarity column used in a wide range of applications from environmental semi-volatile methods through to food and fragrance applications.


0.22 0.25 15 260C129P0.25 0.25 15 260C130P0.25 0.25 30 260C142P0.32 0.25 30 260C143P0.32 0.25 60 260C155P0.25 0.5 30 260C223P0.32 0.5 30 260C224P0.53 0.5 30 260C225P0.53 1 15 260C286P0.53 1 30 260C298P

TR-1701A mid-polarity phase heavily used in environmental analysis as aconfirmational column


0.25 0.25 15 260Q130P0.25 0.25 30 260Q142P0.25 0.25 60 260Q154P0.32 0.25 15 260Q131P0.32 0.25 30 260Q143P0.32 0.25 60 260Q155P0.32 0.5 30 260Q224P

15

TRACE TR-50MSAn ideal selection for GC/MS applications, often used in combinationwith a 5% phenyl column as a confirmation column


0.10 0.10 10 260R020P0.25 0.25 15 260R130P0.25 0.25 30 260R142P0.25 0.25 60 260R154P0.32 0.25 15 260R131P0.32 0.25 30 260R143P0.32 0.25 60 260R155P0.53 0.50 15 260R213P0.53 0.50 30 260R225P

TRACE TR-WAXA highly cross-linked and deactivated column ideal for polar compound analysis, particularly suited to esters, alcohols,ketones, glycols and aromatic isomers.


0.10 0.10 10 260W020P0.25 0.25 15 260W130P0.25 0.25 30 260W142P0.25 0.25 60 260W154P0.25 0.50 30 260W223P0.25 0.50 60 260W235P0.25 1.00 30 260W296P0.32 0.25 15 260W131P0.32 0.25 30 260W143P0.32 0.25 60 260W155P0.32 0.50 30 260W224P0.32 0.50 60 260W236P0.32 1.00 30 260W297P0.32 1.00 60 260W309P0.53 0.50 30 260W225P0.53 1.00 15 260W286P0.53 1.00 30 260W298P0.53 1.00 60 260W310P

TR-WaxMSA highly stable high polarity column suitable for all PEG analyses,with low bleed to enhance sensitivity


0.25 0.25 30 260X142P0.25 0.25 60 260X154P0.25 1.00 30 260X296P0.32 0.25 30 260X143P0.32 0.25 60 260X155P0.32 0.5 30 260X224P0.53 1.00 30 260X298P

TRACE TR-SimDistStrongly cross-linked, high temperature column, specificallydesigned for simulated distillation analysis.


0.1 0.10 10 260S020P0.53 0.10 10 260S025P0.53 0.90 10 260S250P0.53 2.65 6 260S347S0.53 2.65 10 260S348P

TRACE TR-FAMEThe unique selectivity provides excellent separation of cis/transisomers of FAMEs with various degrees of unsaturation


0.10 0.20 10 260M096P0.22 0.25 25 260M135P0.22 0.25 30 260M141P0.22 0.25 50 260M147P0.22 0.25 60 260M153P0.25 0.25 30 260M142P0.25 0.25 60 260M154P0.25 0.25 120 260M166L0.32 0.25 25 260M137P0.32 0.25 30 260M143P0.32 0.25 50 260M149P0.32 0.25 60 260M155P

TRACE TR-V1Ideal for the analysis of volatile compounds including EPA Method624 with good retention and separation at low oven temperatures


0.18 1.00 20 260V495P0.18 1.00 40 260V496P0.25 1.40 30 260V332P0.25 1.40 60 260V333P0.32 1.80 30 260V339P0.32 1.80 60 260V341P0.53 3.00 30 260V396P0.53 3.00 60 260V408P

TRACE Capillary G

C Columns / Syringes

Retention Gap KitsDESCRIPTION PART NO.

Retention Gap Kit (ID 0.1-0.32 mm) 2 m 260RG497(includes connectors)

Retention Gap Kit (ID 0.53 mm) 2 m 260RG499(includes connectors)

Deactivated Silica Tubing, ID 0.32 mm, 2 m 260G498PDeactivated Silica Tubing, ID 0.53 mm, 2 m 260G499P

16

Thermo Syringes

Autosampler Syringes for Thermo Instruments

NEEDLE THERMO INSTRUMENTVOLUME NEEDLE TYPE LENGTH GAUGE TIP STYLE PART NO. TRIPLUS AS3000 AS2000 AS200/AS800 NOTES

0.5 µL Plunger-in-Needle 50 mm 23 Cone 36504045 X X Split/PTV0.5 µL Plunger-in-Needle 75 mm 31 Cone 36500500 Manual OC0.5 µL Plunger-in-Needle 80 mm 26 Cone 36504046 X X5.0 µL Fixed Needle 50 mm 26 Cone 36504047 X Split/PTV5.0 µL Fixed Needle 50 mm 23 Cone 365C3701 X X X5.0 µL Fixed Needle 50 mm 26 Cone 36500505 X X Split/PTV5.0 µl Removable Needle 75 mm 31 Cone 36500510 Manual OC10 µL Removable Needle 42 mm 23 Cone 365D1611 X10 µL Fixed Needle 50 mm 26 Cone 365D1856 X X X X10 µL Fixed Needle 50 mm 25 Cone 36500525 X X X X Split/PTV10 µL Fixed Needle 50 mm 23 Cone 36520060 X X X X BEST PTV/SSL Split10 µL Removable Needle 50 mm 26 Cone 365D1841 X X X10 µL Removable Needle 50 mm 23 Cone 365D3731 X X X X10 µL Fixed Needle 80 mm 23 Cone 36520061 X X Merlin Valve SSL Splitless10 µL Fixed Needle 50 mm 23 – 26 Cone 36500580 X X OC in PTV Merlin Valve10 µL Fixed Needle, Gas Tight 50 mm 23 Cone 365D3741 X X10 µL Fixed Needle 80 mm 26 Cone 36502019 X X OC & Splitless10 µL Removable Needle, Gas Tight 75 mm 31 Cone 36500520 Manual OC50 µL Removable Needle, Gas Tight 50 mm 25 Bevel 365G2161 X50 µL Removable Needle 50 mm 23 Cone 36503015 X LV Splitless100 µL Removable Needle 50 mm 25 Bevel 365H2301 X100 µL Removable Needle, Gas Tight 50 mm 23 Side Hole 36520050 X X100 µL Removable Needle, Gas Tight 50 mm 25 Bevel 365H2381 X100 µL Removable Needle, Gas Tight 50 mm 23 Cone 36500495 X X X LV Splitless250 µL Removable Needle, Gas Tight 50 mm 25 Bevel 365I2561 X250 µL Removable Needle, Gas Tight 50 mm 25 Bevel 365I2611 X250 µL Removable Needle, Gas Tight 80 mm 26 Cone 36500490 X X LVOC250 µL Removable Needle, Gas Tight 50 mm 23 Side Hole 36520051 X X X See note 11.0 mL Removable Needle, Gas Tight 50 mm 23 Bevel 365K3041 X

1. Specific syringe to be used when performing PTV/LVI injections with dedicated liner for thermo labile compounds (p/n 45302060). Compatible with Merlin Microseal™ deviceinstalled on BEST PTV (kit p/n 19050205) and with AS2000 and TriPlus autosamplers for liquids.

The result of any GC analysis is dependent upon the accuracy andprecision of the syringe used to transfer the sample into the GC.Thermo’s syringes are designed, manufactured and inspected toensure the expected volume is transferred every time. Our syringesare made by chromatographers who are familiar with the require-ments of the entire GC system, not just syringe manufacturing.These syringes include fixed and removable needles as well as standard and gas-tight plungers for your convenience.

Thermo Autosampler Syringes

• One source for all GC models• Thermo TriPlus™, AS3000, AS2000, AS200, and AS800• Agilent 7673, 7683 and 6850• PerkinElmer Autosystem and Clarus® 500• Shimadzu AOC 14, 17, 20 and 20i• Varian 8035, 8100, 8200 and 8400• Teflon® Tipped plungers

Prevents plunger binding with dirty samples• Cone point needle tip

Optimized for septum piercing • Field proven designs

Syringe Specifications

Temperature Limits: 5 – 115°C for removable needle syringes; 5 – 70°C for fixed needle syringes

Volume Specifications: Accuracy ±1% (±2% for plunger-in-needle syringes)Reproducibility (displaced volume) ±1% (±2% for plunger-in-needle syringes)

Maximum Safe Working Pressure: 5 µL to 100 µL – 1000 psi250 µL and 500 µL – 500 psi1 mL to 5 mL – 400 psi10 mL – 200 psi25 mL, 50 mL and 100 mL – 100 psi

Maximum Functional Pressure: 100 psi

Materials Used:Gas tight plunger tips – PTFEMetal plungers – stainless steelGlass – borosilicateMetal flanges – stainless steel– nickel plated brass

Needles – stainless steel

17

Syringes Autosampler Syringes for Other Instruments

INSTRUMENT MANUFACTURER VOLUME NEEDLE TYPE NEEDLE LENGTH GAUGE TIP STYLE QTY PART NO.

Agilent 0.5 µL Removable Needle 42 mm 26 Cone 1 365A04110.5 µL Removable Needle 42 mm 23 Cone 1 365A04210.5 µL Removable Needle 42 mm 23 – 26 Cone 1 365A04315 µL Fixed Needle 42 mm 26 Cone 1 365C09215 µL Fixed Needle 42 mm 26 Cone 6 365C09265 µL Fixed Needle 42 mm 23 Cone 1 365C09515 µL Fixed Needle 42 mm 23 Cone 6 365C09565 µL Fixed Needle 42 mm 23 – 26 Cone 1 365C09715 µL Fixed Needle 42 mm 23 – 26 Cone 6 365C097610 µL Fixed Needle 42 mm 26 Cone 1 365D152110 µL Fixed Needle 42 mm 26 Cone 6 365D152610 µL Fixed Needle, Gas Tight 42 mm 23 – 26 Cone 1 365D156110 µL Fixed Needle 42 mm 23 Cone 1 365D157110 µL Fixed Needle 42 mm 23 Cone 6 365D157610 µL Removable Needle, Gas Tight 42 mm 23 Cone 1 365D161110 µL Fixed Needle 42 mm 23 – 26 Cone 1 365D162110 µL Fixed Needle 42 mm 23 – 26 Cone 6 365D1636

PerkinElmer 0.5 µL Removable Needle 70 mm 26 Cone 1 365A04710.5 µL Removable Needle 70 mm 23 Cone 1 365A04815 µL Fixed Needle 70 mm 26 Cone 1 365C10115 µL Fixed Needle 70 mm 23 Cone 1 365C10215 µL Fixed Needle, Gas Tight 70 mm 26 Cone 1 365C10315 µL Fixed Needle, Gas Tight 70 mm 23 Cone 1 365C1041

Shimazdu 10 µL Removable Needle 42 mm 26 Cone 1 365D177110 µL Removable Needle 42 mm 23 Cone 1 365D178110 µL Removable Needle, Gas Tight 42 mm 23 Cone 1 365D1791

Varian 10 µL Removable Needle 53 mm 25 Side Hole 1 365D181110 µL Fixed Needle 50 mm 26 Bevel 1 365D1831

18

Other Syringes

Borosilicate Glass Inert to GC solvents and organics

Stainless SteelInert and non-reactive

Industry Standard SizeConsistent syringe fit

Metal FlangeProtect glass barrel from breakage

Permanent Scale MarkingsSolvents won’t wash off the markings

0.5 µL to 1 mL VolumesWide range for ideal sample take up and delivery

Plunger Protection Prevents plunger bending incidences

White Scale Backing Easy-to-read improves reproducibility

Syringe Specifications

Temperature Limits: 5 – 115°C for removable needle syringes5 – 70°C for fixed needle syringes

Volume Specifications: Accuracy ±1% (±2% for plunger-in-needle syringes)Reproducibility (displaced volume) ±1% (±2% for plunger-in-needle syringes)

Maximum Safe Working Pressure: 5 µL to 100 µL – 1000 psi250 µL and 500 µL – 500 psi1 mL to 5 mL – 400 psi • 10 mL – 200 psi25 mL, 50 mL and 100 mL – 100 psi

Maximum Functional Pressure: 100 psi

Materials Used:Gas tight plunger tips – PTFEMetal plungers – stainless steelGlass – borosilicateMetal flanges – stainless steel – nickel plated brassNeedles – stainless steel

Syringe Selection:Zero dead volume syringes – 0.5 µL and 1 µLStandard plungers – 5 µL to 500 µLGastight plungers – 10 µL to 1 mLFixed needles – 5 µL to 1 mLRemovable needles – 5 µL to 1 mL

Manual GC Syringes

VOLUME NEEDLE TYPE NEEDLE LENGTH GAUGE TIP STYLE QTY PART NO.

0.5 µL Plunger-in-Needle 50 mm 23 Cone 1 365A03311.0 µL Plunger-in-Needle 50 mm 23 Cone 1 365B05111.0 µL Plunger-in-Needle 70 mm 23 Cone 1 365B05315.0 µL Fixed Needle 50 mm 26 Bevel 1 365C07415.0 µL Removable Needle 50 mm 26 Bevel 1 365C076110 µL Fixed Needle 50 mm 26 Bevel 1 365D109110 µL Fixed Needle 50 mm 26 Bevel 6 365D109610 µL Removable Needle 50 mm 26 Bevel 1 365D117125 µL Fixed Needle 50 mm 25 Bevel 1 365F189125 µL Removable Needle 50 mm 25 Bevel 1 365F190125 µL Fixed Needle, Gas Tight 50 mm 25 Bevel 1 365F192125 µL Removable Needle, Gas Tight 70 mm 25 Bevel 1 365F193150 µL Fixed Needle 50 mm 25 Bevel 1 365G208150 µL Removable Needle 50 mm 25 Bevel 1 365G209150 µL Fixed Needle, Gas Tight 50 mm 25 Bevel 1 365G211150 µL Removable Needle, Gas Tight 50 mm 25 Bevel 1 365G2161100 µL Fixed Needle 50 mm 25 Bevel 1 365H2291100 µL Removable Needle 50 mm 25 Bevel 1 365H2301100 µL Fixed Needle, Gas Tight 50 mm 25 Bevel 1 365H2321100 µL Removable Needle, Gas Tight 50 mm 25 Bevel 1 365H2381250 µL Fixed Needle 50 mm 25 Bevel 1 365I2541250 µL Fixed Needle, Gas Tight 50 mm 25 Bevel 1 365I2561250 µL Removable Needle, Gas Tight 50 mm 25 Bevel 1 365I2611500 µL Fixed Needle, Gas Tight 50 mm 25 Bevel 1 365J2831500 µL Removable Needle, Gas Tight 50 mm 25 Bevel 1 365J28811.0 mL Removable Needle, Gas Tight 50 mm 23 Bevel 1 365K30411.0 mL Fixed Needle, Gas Tight 50 mm 22 Bevel 1 365K3051

19

Syringes / Injection Liners

Replacement Needles

PART NO. FOR SYRINGE PART NO. DESCRIPTION

365RN252 365D1611 Pack of 2365RN362 365D1841 Pack of 2365RN272 365D1771 Pack of 2365RN372 365D3731 Pack of 2365RN225 365H2301, 365F1931, 365G2161, Pack of 5

365H2381, 365I2611, 365F1901, 365G2091,365J2881

365RN235 365K3041 Pack of 536566485 36500495, 365D1781, 365D1791, Pack of 2

3650301536566480 36500490 Pack of 136550040 36520050, 36520051 Pack of 1365RN351 365D1811 Pack of 1365RN555 365C0761 Pack of 5365RN215 365D1171 Pack of 536500700 36500510, 36500520 Pack of 2

Replacement Plungers


365RP421 365F1921, 365F1931 Pack of 1365RP451 365G2111 & 365G2161 Pack of 1365RP461 365H2321 & 365H2381 Pack of 1365RP481 365I2561 & 365I2611 Pack of 1365RP491 365J2831 & 365J2881 Pack of 1365RP511 365K3041 & 365K3051 Pack of 1365RP392 365D1561 Pack of 2365RP382 365C1041, 365C1031 Pack of 2365RP522 365D1791 Pack of 2365RP532 365D3741 Pack of 2365RP412 365D1611 Pack of 2365RP471 365H3771 Pack of 1

Repair Kits


365RK541 365A0411 Pack of 1365RK241 365A0421 Pack of 1365RK261 365A0431 Pack of 1365RK291 365A0471 Pack of 1365RK331 365A0481 Pack of 1365RK321 365A0331 Pack of 1365RK311 365B0511 Pack of 1365RK341 365B0531 Pack of 1

Syringe Selection

INJECTOR NEEDLE LENGTHS

Split 51 mmSplitless 70 mmOn Column 75 mm

20

The injection port liner is an essential part of the GC instrument andmust be optimized to ensure sensitive and reproducible analysis.The injection liners from Thermo are highly deactivated, and producedto exacting tolerances to ensure the high level of reproducibilityrequired in today’s separation laboratory. A full range of liners isavailable for Thermo and other manufacturers’ instruments.

Thermo FocusLiner™ GC Injection LinerThe Thermo FocusLiner contains a quartz wool filter that improvesthe rate of vaporization, reducing the discrimination of high boilinganalytes in the sample. It also prevents non-volatile componentsfrom entering the column, improving sensitivity and extending columnlifetimes. The FocusLiner secures the quartz wool between tworestrictions preventing movement during syringe needle injection.

This allows the quartz wool to consistently wipe the dispensed liquid away from the end of the syringe needle during the injectionprocess. This results in lower carryover and greatly improved precision,and Relative Standard Deviation (RSD) of less than 1% can be achieved.

GC Injection Liners

Note: Liners not drawn to scale.

21

Liners for Thermo Range of InstrumentsINSTRUMENT I.D. X O.D. X LENGTH (mm) INJECTION TYPE PACK PART NO.

TRACE & FOCUS SSL 5 x 8 x 105 Splitless 5 453T2895Use 70 mm Needle

TRACE & FOCUS SSL 5 x 8 x 105 Split 5 453T1905Use 50 mm Needle

TRACE & FOCUS SSL 3 x 8 x 105 Split 5 45350031

TRACE & FOCUS SSL 5 x 8 x 105 Split 5 45350030

TRACE & FOCUS SSL 3 x 8 x 105 Splitless 5 45350032

TRACE & FOCUS SSL 5 x 8 x 105 Splitless 5 45350033

Thermo TRACE & Focus PTV 1 x 2.75 x 120 PTV 5 45352054




Mega 4000, 5000, 6000 3 x 5 x 79.5 Split 5 45300400

Mega 4000, 5000, 6000 5 x 5 x 79.5 Split/Splitless 5 453T2955

Mega 4000, 5000, 6000 2 x 5 x 79.5 Split 5 45300300


22

Injection Liners

Other LinersINSTRUMENT I.D. X O.D. X LENGTH (mm) PACK OF 5 PACK OF 25

Agilent 4.0 x 6.3 x 78.5 453A1255 453A1252

Agilent 2.3 x 6.3 x 78.5 453A1285 453A2282

Agilent 2.3 x 6.3 x 78.5 453A2375 453A1372

Agilent 4.0 x 6.3 x 78.5 453A1925

Agilent 4.0 x 6.3 x 78.5 453A2265 453A1262

Agilent 4.0 x 6.3 x 78.5 453A1345 453A2342

Agilent 4.0 x 6.3 x 78.5 453A1305 453A2302

Agilent 4.0 x 6.3 x 78.5 453A1355 453A2352

Agilent 4.0 x 6.3 x 78.5 453A1315 453A1312

Agilent 4.0 x 6.3 x 78.5 453A1295 453A2292

Agilent 1.2 x 6.3 x 78.5 453A1335 453A2332

Agilent 2.0 x 6.1 x 78.5 453A2275

Agilent 2.0 x 6.3 x 78.5 453A2325

Varian 1078/1079 0.75 x 5 x 54 453V2995

Varian 1078/1079 2 x 5 x 54 453V2545

Varian 1078/1079 3.4 x 5 x 54 453V1535

Varian 1078/1079 0.5 x 5 x 54 453V2885

Varian 1078/1079 3.4 x 5 x 54 453V1515

Varian 1078/1079 3.4 x 5 x 54 453V2525

Shimadzu 2010 3.4 x 5 x 95 453S2985

Shimadzu 2010 3.4 x 5 x 95 453S2615

Shimadzu 2010 2.6 x 5 x 95 453S1645

Shimadzu 17A 3.4 x 5 x 95 453S1605

Shimadzu 17A 3.4 x 5 x 95 453S1625

Shimadzu 17A 3.4 x 5 x 95 453S2595

Shimadzu 17A 3.4 x 5 x 95 453S2615

Other LinersINSTRUMENT I.D. X O.D. X LENGTH (mm) PACK OF 5

Shimadzu 17A 3.4 x 5 x 95 453S2635

Shimadzu 17A 2.6 x 5 x 95 453S1645

Shimadzu 17A 0.75 x 5 x 95 453S1665

PE Clarus 2 x 4 x 86.2 453P1795

PE Clarus 4 x 6.2 x 92 453P1755

PE Clarus 4 x 6.2 x 92 453P2765

PE Clarus 4 x 6.2 x 92 453P2785

PE Clarus 2 x 6.2 x 92 453P2805

PE Clarus 2 x 4 x 86.2 453P1775

PE Clarus 3 x 6 x 112 453P1815

Liner Sealing Rings

INSTRUMENT LINER MATERIAL PACK SIZE PART NO.

Thermo 8 mm O.D. Liners Graphite Ring 10 29033406 Agilent 453A1335, 453A1355, Graphite Ring 10 290GA242

453A2332, 453A2352 Agilent 453A2265, 453A1255, 453A1315, 453A1285, 453A1295, 453A2282, 453A1305, Graphite Ring 10 290GA243

453A1312,453A2325, 453A1345, 453A2375, 453A1372, 453A1252, 453A1262, 453A2292, 453A2302, 453A2342

Agilent 453A2275 Graphite Ring 10 290GA244 Shimadzu 2010 453S2985 Viton® O-Ring 10 290OS246 Shimadzu 17A All Liners Graphite Ring 10 290GS247Varian 1078/1079 All Liners Graphite Ring 10 290GV248

23


FerrulesFerrules from Thermo are available in 3 different materials, in variousdimensions to match the instrument and capillary column ID. Thechoice of material is dependent upon the use; some guidelines aregiven below:

MATERIAL TYPE SUITABLE FOR GC/MS TEMP LIMIT (°C) RE-USABLE

Graphite No 450 YesGraphite / Vespel Yes 350 NoStainless steel (SilTite) Yes 500 No

100% Graphite Ferrules• Easy-to-use• Provides a stable seal• Soft material – can be squashed• Porous to O2 (not for GC/MS)• Temperature Limit 450°C• Forms ‘soft’ grip with column• Reusable

INSTRUMENT COLUMN SIZE (ID) PACK SIZE PART NO.

TRACE/FOCUS 0.1 – 0.25 mm 10 29013488TRACE/FOCUS 0.32 mm 10 29013487TRACE/FOCUS 0.53 mm 10 29013486Agilent (Not MSD) 0.10 – 0.32 mm ID 10 290GA139Agilent (Not MSD) 0.45 – 0.53 mm ID 10 290GA140Varian (Not GC/MS) 0.10 – 0.32 mm ID 10 290GP132Varian (Not GC/MS) 0.45 – 0.53 mm ID 10 290GP131

15% Graphite / 85% Vespel Ferrules• Mechanically robust• Hard material, long lifetime• Temperature limit 350°C• Must re-tighten after initial

temperature cycles• Forms strong grip with column• Cannot be reused with another

capillary column• For GC/MS interface


TRACE On Column 0.1 – 0.25 mm 10 29033461TRACE On Column 0.32 mm 10 29033460TRACE On Column 0.53 mm 10 29033471TRACE SSL 0.1 – 0.25 mm 10* 290VT186TRACE SSL 0.32 mm 10* 290VT187TRACE SSL 0.53 mm 10* 290VT188Agilent (Not MSD) 0.10 – 0.25 mm 10 290VA191Agilent (Not MSD) 0.32 mm 10 290VA192Agilent (Not MSD) 0.45 – 0.53 mm 10 290VA193Varian (Not GC/MS) 0.10 – 0.25 mm 10 290VP156Varian (Not GC/MS) 0.32 mm 10 290VP141Varian (Not GC/MS) 0.45 – 0.53 mm 10 290VP144

* Use with Nut 290BT239

Septa and FerrulesAlthough they are small consumables for GC, the quality of ferrulesand septa are essential for chromatographic performance. Theseconsumables are in the flow path of the GC and therefore need to beinert, free from contaminants, and have tight dimensional requirements.

For this reason the septa and ferrules are supplied in contaminant-free individual blister packs, and one item can be removed at a timewithout risk of contamination to the other supplied items.

24

Septa and Ferrules

SilTite™ Metal Ferrules• Forms a strong, permanent, airtight

seal around the capillary column,eliminating leaks

• Ferrule and nut are made from thesame material and have the samethermal expansion coefficient

• Eliminates the need to re-tighten the ferrule after installation• Base of the ferrule is flat and forms a perfect seal with the

MS interface• Temperature tolerance well above the limit of the injector,

MS interface or GC oven• Available for most major GC/MS instrument interfaces

SilTite Starter Kits


TRACE/FOCUS 0.1 – 0.25 mm 10 290MT229TRACE/FOCUS 0.32 mm 10 290MT230TRACE/FOCUS 0.53 mm 10 290MT231Agilent MSD 0.10 mm – 0.25 mm ID 1 290MA215Agilent MSD 0.32 mm ID 1 290MA216Agilent MSD 0.45 mm – 0.53 mm ID 1 290MA217Varian GC/MS 0.10 – 0.25 mm ID 1 290MV218Varian GC/MS 0.32 mm ID 1 290MV219Varian GC/MS 0.45 – 0.53 mm ID 1 290MV220

(Kit contains 2 Nuts & 10 Ferrules)

Replacement SilTite FerrulesINSTRUMENT COLUMN SIZE (ID) PACK SIZE PART NO.

TRACE/FOCUS 0.1 – 0.25 mm 10 290MT221TRACE/FOCUS 0.32 mm 10 290MT222TRACE/FOCUS 0.53 mm 10 290MT223Agilent MSD 0.10 mm – 0.25 mm ID 10 290MA201Agilent MSD 0.32 mm ID 10 290MA202Agilent MSD 0.45 mm – 0.53 mm ID 10 290MA203Varian GC/MS 0.10 – 0.25 mm ID 10 290MA201Varian GC/MS 0.32 mm ID 10 290MA202Varian GC/MS 0.45 – 0.53 mm ID 10 290MA203

SeptaSepta are an important part of the GCinstrument and require good durability,resealability and low bleed to optimizethe performance of your instrument.Septa from Thermo are available inseveral types of materials described below to fit in Thermo andother manufacturers’ instruments.

SEPTA TYPE DESCRIPTION TEMPERATURE LIMIT (°C)

TCS Triple layer with Teflon 280CS Silicone with Teflon 200Auto-Sep™ High Grade Silicone 320Auto-Sep T™ High Grade Silicone with Teflon 350BTO® Low Bleed Silicone 400

Septa for use with Thermo TRACE and FOCUS InjectorsINJECTOR SEPTA MATERIAL DIAMETER PACK SIZE PART NO.

Split/Splitless BTO 17 mm 50 31303211Packed BTO 11.5 mm 50 31303230Large Volume SL BTO 9 mm 50 31303240PTV BTO 12.7 mm 10 31313225

Other Compatible Septa(also for use with other instruments)

SEPTA MATERIAL DIAMETER PACK SIZE PART NO.

CS 11 mm 50 313C110MCS 12.5 mm 50 313C125ATCS 11 mm 50 313T110MTCS 12.5 mm 50 313T125ATCS 17 mm 50 313T170TAuto-Sep 9.5 mm 25 313A950MAuto-Sep 11 mm 25 313A110VAuto-SepT 9.5 mm 25 313S950MAuto-SepT 11 mm 25 313S110MAuto-SepT 11 mm 100 313S119MEnduro Shimadzu plug 50 313B201S

25

Thermo provide vials tested on the our range of GC instruments autosamplers;however they are compatible with many other manufacturers instrumentssuch as Agilent, PerkinElmer and Varian. Made from highly pure borosilicateglass, the vials meet the high standards required by such bodies as the US &European Pharmacopoeia, and ASTM for hydrolytic extraction.

DIMENSIONSDESCRIPTION mm x mm PART NO.

300 µL micro-volume sampling vials, set of 100 (require screw top cap 12 x 32 60180-507 Xwith septa p/n 60180-516)Crimp top vials 1 mL, 8 mm finish (Set of 100), requires 8 mm aluminum 8 x 40 60180-500 X XCaps and seals (60180-525) and plastic reducer (60180-524)Crimp top vials 1.2 mL, 8 mm finish (Set of 100), requires 8 mm aluminum 8 x 40 60180-501 X XCaps and seals (60180-525) and plastic reducer (60180-524)2 mL screw top vials, set of 100 (require screw top cap with septa 12 x 32 60180-509 X Xp/n 60180-516)Screw top vials, 2 mL, 8 mm finish, (set of 100), require 8 mm plastic screw 12 x 32 60180-508 X Xcaps 60180-514 (set of 100), Teflon faced seals, plastic screw caps 60180-514 (set of 100), Teflon faced seals, 60180-515 (set of 100) and plastic spacers, 60180-523 (set of 100)Crimp top vials 2 mL, 11 mm finish (set of 100), require 11 mm aluminum 12 x 32 60180-502 X Xcaps and seals (60180-526) and spacers 60180-5234 mL solvent vials, set of 100 (require screw top cap with septa 15 x 46 60180-510 Xp/n 60180-516)Crimp top vials, 10 mL, 20 mm finish (set of 125), require 20 mm aluminum 22 x 45 60180-504 X X X X Xcaps and seals (60180-513 or 60180-511)Crimp top amber vials, 10 mL, 20 mm finish (set of 125), require 20 mm 22 x 45 60180-505 X X X X Xaluminum caps and seals (60180-513 or 60180-511)Crimp top vials, 20 mL, 20 mm finish (set of 125), require 20 mm aluminum 22 x 75 60180-506 X X Xcaps and seals (60180-513 or 60180-511)Note: Although suitable for many autosamplers, these vials are tested for Thermo autosampler compatibility.

HEADSPACE VIALS

Crimp top vials, 10 mL, 20 mm finish (set of 125), require 20 mm aluminum caps 60180-504 X X X X Xand seals (60180-513 or 60180-511)Crimp top amber vials, 10 mL, 20 mm finish (set of 125), 60180-505 X X X X Xrequire 20 mm aluminum caps and seals (60180-513 or 60180-511)Crimp top vials, 20 mL, 20 mm finish (set of 125), require 20 mm aluminum caps 60180-506 X X Xand seals (60180-513 or 60180-511)Aluminum crimp cap with chlorobutyl/PTFE seal 60180-513 X X X X XAluminum crimp cap with silicone/PTFE seal 60180-511 X X X X X

Vials

AI/A

S300

0 AS

800/

AS20

00HS

800

HS85

0/HS

2000

TRIP

LUS

HSTR

IPLU

S AS

26

Vials / Caps and Septa

DESCRIPTION PART NO.

Screw top caps for 2 mL, 4 mL and 300 µL vials complete with septa, set of 100 60180-516Aluminum crimp cap with chlorobutyl/PTFE seal 60180-513Aluminum crimp cap with silicone/PTFE seal 60180-511Plastic screw caps, 8 mm (set of 100) 60180-5148 mm, Teflon faced seals (set of 100) 60180-515Plastic spacers for 2 mL screw top vils (set of 100) 60180-523Plastic reducer for 1 and 1.2 mL vials pack of 10 60180-524Aluminum caps 8 mm with Teflon faced Septa 60180-525Aluminium crimp cap, 11 mm with Teflon faced Septa 60180-526Plastic reducer for 2 mL screw top vials pack of 100 60180-523

Caps and Septa

DESCRIPTION PART NO.

Straight tipped forceps 60180-770Angle tipped forceps 60180-771Pin vice for drilling holes in ferrules 60180-772Magnifying glass 60180-773Probes 60180-774Inspection mirror 60180-775Needle file 60180-776Pick-up tool 60180-777Injector cleaning brush 60180-778Column cutter 60201-318Complete boxed kit 60180-779

General GC Tools

Crimping

DESCRIPTION USA UK EUR JAPAN

Electronic Crimper 11 mm 60180-527 60180-528 60180-529 60180-530Electronic Crimper 20 mm 60180-531 60180-532 60180-533 60180-534

Decapping

DESCRIPTION USA UK EUR JAPAN

Electronic Decapper 11 mm 60180-535 60180-536 60180-537 60180-538Electronic Decapper 20 mm 60180-539 60180-540 60180-541 60180-542

27

©2005 Thermo Electron Corporation. All rights reserved. Microseal is a trademark of Merlin Instruments. Viton is a registered trademark of DuPont Dow ElastomersL.L.C..Vespel and Teflon are registered trademarks of E.I. DuPont de Nemours and Company. FocusLiner, SilTite, Auto-Sep, and Auto-Sep T are trademarks of SGEInternational Pty. Ltd.. Clarus is a registered trademark of PerkinElmer Instruments LLC. BTO is a registered trademark of Chromatography Research Supplies. All other trademarks are the property of Thermo Electron Corporation and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in allcountries. Please consult your local sales representative for details.

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Switzerland +41 61 48784 00UK +44 1442 233555USA +1 800 532 4752

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