Collection and Collection and Curation Curation of of Fleshy Fungi Fleshy Fungi Clark L. Clark L. Ovrebo Ovrebo Department of Biology Department of Biology University of Central Oklahoma University of Central Oklahoma Edmond, OK 73034 Edmond, OK 73034
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Collection and Collection and CurationCuration of of Fleshy FungiFleshy Fungi
Clark L. Clark L. OvreboOvreboDepartment of BiologyDepartment of Biology
University of Central OklahomaUniversity of Central OklahomaEdmond, OK 73034Edmond, OK 73034
Few collections of fleshy fungi preserved Few collections of fleshy fungi preserved in herbaria of TX and OKin herbaria of TX and OKLack of Mycologists who study taxonomy Lack of Mycologists who study taxonomy of fleshy fungiof fleshy fungiLack of knowledge of how to collect and Lack of knowledge of how to collect and preserve collections preserve collections
larger cup fungi (various orders) larger cup fungi (various orders)
Excluded from this presentation:Excluded from this presentation:plant pathogens, water molds, fungus culturesplant pathogens, water molds, fungus cultures
Wrap specimens in wax paper or Al foilWrap specimens in wax paper or Al foilkeep cool in hot weatherkeep cool in hot weather
When collecting . . .When collecting . . .
Site location Site location –– state, county, locality, GPSstate, county, locality, GPSGrowth habit Growth habit –– solitary, gregarious, solitary, gregarious, caespitosecaespitoseSubstrate Substrate –– soil, lawn, wood (logs and soil, lawn, wood (logs and sticks, mulch), living tree, dungsticks, mulch), living tree, dungForest type, tree associates Forest type, tree associates
Preserve by drying, but appearance of Preserve by drying, but appearance of sporocarpssporocarpschanges substantially upon dryingchanges substantially upon drying
Macroscopic features must be recorded when freshMacroscopic features must be recorded when fresh
changes during maturationchanges during maturationLamellae size, attachment, color, Lamellae size, attachment, color,
discolorationsdiscolorationsStipeStipe size, shape, color, discolorationssize, shape, color, discolorationsPresence/absence of partial or universal Presence/absence of partial or universal
veilveilOdor and tasteOdor and tasteChemical spot testsChemical spot tests
Make a spore printMake a spore printuse white paperuse white paper
Preserve by dryingPreserve by drying
Storage with labelsStorage with labels
Dried tissue suitable for DNA extractionDried tissue suitable for DNA extractionPreserve a small piece of Preserve a small piece of pileuspileus/lamellae in silica /lamellae in silica gelgel
LiteratureLiterature
Field guides Technical literature/monographs
Mushroom Clubs/ForaysMushroom Clubs/ForaysNorth American Mycological Association North American Mycological Association Texas Mycological SocietyTexas Mycological SocietyNew Mexico Mycological SocietyNew Mexico Mycological SocietyGulf States Mycological SocietyGulf States Mycological Society
Summer ForaySummer ForayWinter Foray, DecemberWinter Foray, December
ConclusionsConclusionsProgress is slow in documenting macroProgress is slow in documenting macro--fungi diversityfungi diversityEphemeral nature, dependence on Ephemeral nature, dependence on moisture moisture Many areas poorly documentedMany areas poorly documentedYou do not have to be a Mycologist to You do not have to be a Mycologist to collect and preserve fungi, e.g., collect and preserve fungi, e.g., contribution of field biologists, amateurscontribution of field biologists, amateurs