Co-culture of adult human keratinocytes and human · Web viewSupplementary Material Strategies for improving the physiological relevance of human engineered tissuesRosalyn D. Abbott,

Supplementary Material Strategies for improving the physiological relevance of human engineered tissuesRosalyn D. Abbott, David L. KaplanDepartment of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA 02115, USA Corresponding author: Kaplan, D.L. ([email protected])

Supplementary Table 1. Long term culture in microfluidic platforms

2D or 3D? Application Cell Type

Length of culture

Reference

2D

Drug screening for cancer research

T98G human glioblastoma tumor cells

60h [S1]

Human T-cell lymphoma HuT 78 cellsHuman Burkitt s lymphoma ′Ramos cellsHuman prostate adenocarcinoma PC-3 cellsMurine endothelial C166-GFP cells

2d2d2d7d [S2]

A549 nonsmall lung cancer cells 11d [S3]Human carcinoma HeLa cells 16d [S4]Human carcinoma HeLa cells 2d [S5]

Neural network connectivity and/or

neuropharmacological studies

Lund human mesencephalic (LUHMES) cellsNeuroblastoma SH-SY5Y cellsMurine cortical cells

7d13d13d [S6]

Hippocampal neurons 12d [S7]Co-culture of rat primary embryonic cortical cells and thalamic neurons

35d[S8]

Neurons from murine embryos 18d [S9]Primary embryonic rat cortical neurons

35d [S10]

Co-culture of neurons and glia cells

21d [S11]

Primary murine embryonic neuronal cultures

14d [S12]

Embryonic rat dorsal root ganglion neurons and cortical neurons

2d[S13]

Intestine toxicity and Human intestinal Caco-2 cells 16d [S14]

drug screeningLiver toxicity and drug

screeningRat hepatocytes 9d [S15]Human hepatocytes 32d [S16]

Dynamic stimulation

Human carcinoma HeLa cellsHamster ovarian cancer CHO-K1 cellsMurine 3T3 fibroblast

3d3d1d [S17]

Preadipocyte Differentiation Murine 3T3-L1 preadipocytes 14d [S18]

Cardio-myogenic differentiation

Murine embryoid cell bodies 4d [S19]Human umbilical artery vascular smooth muscle cells

14d [S20]

Human induced pluripotent stem cell derived cardiomyocytes

21d [S21]

Skeletal muscle differentiation

Murine C2C12 skeletal muscle cells

14d [S22]

Murine C2C12 skeletal muscle cells

14d [S23]

Vascular hemodynamics Bovine artery endothelial cells 6d [S24]

Artificial skin development

Human neonatal dermal fibroblasts

21d [S25]

Single cell monitoring

Human SW480 epithelial cellsHuman HT29 colon carcinoma cellsMCF-7 human breast cancer cells(Co-culture experiments at the single cell level – homotypic and herterotypic)

5d5d5d

[S26]

Human leukemic monocyte lymphoma U937 cellsHuman Jurkat T-lymphoblast cells(non-adherent cells)

4d

4d [S27]

T-cell lymphoma EG7 cells(non-adherent cells)

12h [S28]

Platform development

Murine C2C12 skeletal muscle cells(controlled O2 microenvironment)

3d[S29]

Murine C2C12 skeletal muscle cells(integration of track-etched membranes)

14d

[S30]

Human embryonic kidney 293 5d [S31]

cells(a stage perfusion incubation system)HepG2/C3A human hepatoma cells (prevent/remove bubbles)

3d [S32]

Amniotic fluid mesenchymal stem cells(differentiation under continuous flow)

21d

[S33]

Bovine pulmonary artery endothelial cellsLung microvessel endothelial cells(portable automated bench-top mammalian cell-based toxicity sensor)

9d112d

[S34]

Monkey COS7 kidney cells(thermomodulated cell culture and harvest without trypsin)

3d[S35]

Human malignant melanoma A375 cells (concentration gradients)

36h[S36]

Murine MC 3T3 E1 cells(bubble-free, on-chip culture of cells)

10d[S37]

Murine fibroblasts(portable cell culture device)

7d [S38]

Pheochromocytoma PC12 cells of the rat adrenal medulla(time lapse imaging device that tracks live cells)

7d

[S39]

Murine embryonic stem cellsMurine C2C12 skeletal muscle cellsHuman foreskin fibroblasts(periodic perfusion for homogenous cell culture)

6d6d50d [S40]

Monkey CV-1 kidney epithelial cells(portable cell culture device)

12d[S41]

Murine pancreatic islets(prevent/remove bubbles)

1d [S42]

Murine 3T3 fibroblastMurine connective tissue L929

4d4d

[S43]

cellsHuman osteosarcoma MG63 cells(establishment of an air–liquid interface)

4d

Metastatic human breast cancer MDA-MB-231 cells(patterning and differentiation of cells)

12h

[S44]

Murine MC3T3-E1 osteoblastic cellsMurine C2C12 skeletal muscle cells(portable cell culture device)

6d16d

[S45]

Human umbilical endothelial cellsRat pheochromocytoma PC12(electrochemical monitoring of cultured cells)

14d10d [S46]

Human mesenchymal stem cells(patterning and differentiation of cells)

21d[S47]

Murine connective tissue L929 cells(combined chemical and mechanical gradient)

44h

[S48]

Human foreskin fibroblastsHuman umbilical vein endothelial cellsCo-culture of human foreskin fibroblasts and human embryonic stem cells(“flow-stop” perfusion system for shear sensitive cells to withstand medium renewal flow)

14d14d24h

[S49]

Murine endothelial RCL-2583 cellsHuman T lymphocyte HuT 78 cellsHuman B lymphocyte RPMI 8226 cells(stable, homogeneous, and low-shear force environment)

4d4d4d

[S50]

Human lung adenocarcinoma CL1-5 cells(integrated heater and a

122h [S51]

concentration gradient generator)MCF-7 human breast cancer cellsHuman mesenchymal stem cells(pulsatile pumping system for cell delivery and culturing functions on a single platform)

7d7d

[S52]

Human melanoma A2058Murine Cath.a-differentiated cellsHuman bladder epithelial T24 cells(closed mini chamber system that supports long term time lapse recordings)

229h28d1 d

[S53]

Murine embryonic neural progenitor cells(on-chip transfection of adherent cells by localized electroporation)

6d

[S54]

3D Toxicity and drug screening

MCF-7 human breast cancer cells 6d [S55]Human LS174T colon carcinoma 4d [S56]Non-tumorigenic human breast MCF10A cellsMCF-10A pBabeRas-transformed MCF-10AT1Tumorigenic MCF-10CA1aTumorigenic MCF-10CA1d

21d

21d21d21d21d

[S57]

HT-29 human colon carcinoma cells

25d [S58]

Non-tumorigenic human breast MCF10A cellsMCF-7 human breast cancer cells

12d

12d[S59]

Human LCC6/Her2 breast tumor cells

6d [S60]

Human colon cancer HCT-116 cells

24h [S61]

Human LCC6/Her-2 breast tumor cells

9d [S62]

Human colon carcinoma HT-29 cellsHuman liver carcinoma Hep-G2 cells

12d10d [S63]

MCF-7 human breast cancer cells 6d [S64]Murine Col1a1GFP MC-3T3 E1 10d [S65]

osteoblastic cells

Vasculature

Co-culture of human umbilical vein endothelial cells, normal human lung fibroblasts, and either human HL-60 promyelocytic leukemia cells, or human U87MG glioblastoma multiforme cells (microvasculature creation through gels)

7d

[S66]

Human dermal microvascular endothelial cells (microvasculature creation through tubes)

32d

[S67]

Mice resistance arteries(ex vivo artery perfusion and superfusion system)

24h[S68]

Primary human umbilical vein endothelial cells(micropatterning of 3D natural ECM of flexible compositions and gelling mechanisms)

21d

[S69]

Neural

Co-culture of 2 rat hippocampus slices with a microchannel axonal connection between them

22d[S70]

Co-culture of rat or murine hippocampus slices with axonal extensions

28d[S71]

Platform development

Primary human periodontal ligament cells(high-resolution bioimaging)

28d[S72]

Cardio-myogenic differentiation Murine embryoid cell bodies 4d [S19]

Liver Human hepatoma cell line C3A 4d [S73]Rat hepatocytes 90d [S74]Co-culture of primary rat hepatocytes and murine swiss 3T3 cells feeder cells

90d[S75]

Rat hepatocytes 14d [S76]Human liver carcinoma HepG2 cells

14d [S77]

Co-culture of human liver carcinoma HepG2 cells and

5d [S78]

murine Balb/c 3T3 fibroblast cells

Skin

Co-culture of human keratinocytes, fibroblasts and endothelial cells

14d[S79]

Co-culture of human foreskin keratinocytes and fibroblasts

3w (21d) [S80]

Supplementary Table 2. Long term culture in static three dimensional cultures

Application Cell Type Length of culture

Reference

Neural

Co-culture of murine spinal cord motor neurons, murine fibroblasts, and murine schwann cells

28d [S81]

Human umbilical cord blood stem cells 73d [S82]Adult murine neural stem cells 5m (150d) [S83]Co-culture of murine D3 embryonic stem cells, murine CGR8 embryonic stem cells, and murine MS5 bone marrow-derived stromal feeder cells

3.5m (105d)

[S84]

Intestine Small intestine explants of GFP C57BL/6 or wild-type C57BL/6J neonatal mice

14d [S85]

Tendon Human tenocytes 28d [S86]Cancer spheroids Human epithelial ovarian carcinoma cells 28d [S87]

Cardiovascular

Co-culture of primary neonatal rat ventricular cardiomyocytes, murine 3T3-J2 and primaryrat heart microvessel endothelial cells

28d [S88]

Murine cardiomyocyte HL1 cell line 38d [S89]Fetal rat cardiocytes 84d [S90]Myocardial tissue from newborn mice 56d [S91]Primary neonatal murine cardiomyocytes 25d [S92]

Skin

Co-culture of adult human epidermal keratinocytes and human dermal fibroblasts

95d [S93]

Co-culture of adult human keratinocytes and human dermal fibroblasts

84d [S94]

Co-culture of murine epidermal keratinocytes and murine dermal fibroblasts

14d [S95]

Co-culture of normal human dermal fibroblasts and normal human epidermal keratinocytes(cultured in human fibroblast-derived matrix)

140d [S96]

Human epidermal keratinocytes(epidermal gene therapy)

7d [S97]

Retinal Murine embryonic stem cells 24d [S98]

Adipose Rat bone marrow stromal cells 28d [S99]Bone and Adipose Human mesenchymal stem cells 50d [S100]

Bone Rabbit bone marrow stromal cells 35d [S101]Murine periosteum mesenchymal stem cells 365d [S102]Co-culture of human umbilical vein endothelial cells and primary human osteoblasts

42d [S103]

Human bone marrow derived mesenchymal stem cells

46d [S104]

Human cord blood mononuclear cells 28d [S105]Osteochondral Human mesenchymal stem cells 45d [S106]

Cartilage

Human bone marrow derived stem cells 84d [S107]Human bone marrow stromal cells and human neonatal and adult chondrocytes(dynamic seeding for 24 hours)

42d [S108]

Human mesenchymal stem cells(non-viral gene therapy)

21d [S109]

Rat primary chondrocytes(lentiviral gene therapy)

56d [S110]

Porcine femoral condylar chondrocytes 42d [S111]Bovine articular chondrocytes 52d [S112]Porcine articular chondrocytes 35d [S113]Bovine chondrocytesBovine mesenchymal stem cells

70d70d

[S114]

Bovine articular chondrocytes 9d [S115]Chondrocytes (porcine and equine) 6m (180d) [S116]Bovine juvenile articular chondrocytes 35d [S117]

Liver

Co-culture of rat hepatocytes and primary rat hepatic stellate cells

54d [S118]

Human fetal hepatocytes 35d [S119]Murine fetal liver cells 35d [S120]Murine fetal liver cells 245d [S121]Human hepatocellular carcinoma-derived FLC-4 24d [S122]Adult rabbit primary biliary epithelial cells 3m (90d) [S123]Co-culture of rat hepatocytes, rat hepatic stellate cells, and bovine pulmonary microvascular endothelial cells

40d [S124]

Human hepatocyte carcinoma (HepG2) 27d [S125]Pancreas Human islets 10d [S126]

Kidney Co-culture of murine glomerular epithelial and mesangial cells

1m (30d) [S127]

Oropharyngeal mucosa

Co-culture of human oropharyngeal keratinocytes and fibroblasts

10d [S128]

General 3D encapsulation methods that

could be applied to multiple

tissues

Murine fibroblasts(cross-linked collagen gels)

42d [S129]

Porcine chondrocytesHuman dermal fibroblasts(collagen gels)

7d7d

[S130]

Murine ATDC5 chondrocytes(mild cell encapsulation method based on self-assembly and microfluidics technology)

21d [S131]

Human trophoblast HTR-8/SVneo cells 45d [S132]

Rat aorta vascular smooth muscle cellsSheep aortic endothelial cellsHuman umbilical vein endothelial cells(modular approach that could be paired with endothelialized, perfusable channels and perfusion in some experiments, see Table 3)

14d14d14d

Murine connective tissue L929 cells and normal human dermal fibroblasts(fibronectin/gelatin multilayered tissues)

28d [S133]

Murine 3T3 fibroblasts(dense fibrillar collagen scaffold)

7d [S134]

Normal human dermal fibroblasts(Silica nanoparticle–collagen bionanocomposites)

21d [S135]

Co-culture of bovine fibroblasts and bovine mesenchymal stem cells(photo-patterned hydrogels)

14d [S136]

Rat primary bladder smooth muscle cells(multilayered printing of 3D cell-laden collagen hydrogel structures)

14d [S137]

Murine embryonic stem D3 cells(polyethylene terephthalate fibrous matrices)

28d [S138]

Supplementary Table 3. Long term culture in large scale 3D bioreactors

Type of Bioreactor Cell Type (application)

Length of

cultureReference

Direct Perfusion

Human primary tonsil cells(packed bed bioreactor studying peripheral lymphoid structure and function)

42d [S139]

Rat primary hepatocytes(packed bed bioreactor for creating a liver tissue model)

14d [S132]

Human mesenchymal stem cells(packed bed of alginate beads for determining the effects of shear stresses on differentiating hMSCs)

28d [S140]

Murine C2C12 skeletal muscle cells(cell cycle analysis to study relevant population markers)

10d [S141]

Murine C2C12 skeletal muscle cellsSkeletal-muscle precursor cells(platform development for a cellularized artificial graft with high density of viable cells and uniform cell distribution)

7d7d

[S142]

Sheep mesenchymal stem cell(platform development for the maintenance of critically sized scaffolds)

28d [S143]

Human mesenchymal stem cells(stem cell tissue engineering)

40d [S144]

Primary rat osteoblastsRat bone marrow stromal cells(bone model for improved viability)

42d42d

[S145]

Human chondrocytes(generation of artificial connective tissue)

40d [S146]

Human adipose derived mesenchymal stem cells(differential pressure laminar flow reactor for osteogenic differentiation towards a tissue-engineered bone substitute)

60d [S147]

Perfusion + mechanical stimulation

Bovine calf articular chondrocytes(cartilage cultivation for functional tissue engineering)

67d [S148]

Mechanical Simulation

Rat bone marrow stromal cells(four point bending of natural or engineered tissues in vitro)

16d [S149]

Porcine annulus fibrosus cells(cyclic compressive platform for enhanced matrix production)

14d [S150]

Human bone marrow derived and adipose derived stem cells(cyclic hydrostatic cultures for cartilage tissue engineering)

21d [S151]

Indirect Perfusion

Human liver carcinoma HepG2 cells(radial flow high cell density liver model for drug testing/regenerative medicine)

17d [S152]

Co-culture of primary porcine hepatocytes and nonparenchymal cells(hollow fiber bioreactor for liver regeneration and tissue formation)

30d [S153]

Primary osteoblasts isolated from embryonic chick calvaria(hollow fiber bioreactor for studying bone formation)

63d [S154]

Primary human hepatocytes(hollow fiber bioreactor for liver metabolism and toxicity studies)

21d [S155]

Rotating wall vessel

Rat neural stem cells and neural progenitors(neural model for cell replacement therapy)

63d [S156]

Human liver carcinoma HepG2 cells(liver tissue model)

70d [S157]

Bovine articular chondrocytes(pseudo-weightlessness in prolonged immobilization, hydrotherapy, and intrauterine development)

210d [S158]

Dura mater stem cells from a rat fetus(bone mineralization model)

28d [S159]

Spinner flask

Co-culture of human endothelial cells and human adipose derived stem cells(adipose silk scaffold model for long term sustainable function)

180d [S160]

Murine embryonic stem D3 cells (ATCC CRL-1934)(fibrous bed for long term expansion of embryoid bodies)

15d [S161]

Orbital shaker

Bovine mesenchymal stem cellsBovine chondrocytes(cartilage model comparing chondrocytes to MSCs for functional repair)

112d112d

[S162]

Rat marrow stromal cells(bone model to investigate dose patterns under dynamic stimulation)

32d [S163]

Primary rat hepatocytes(hollow fiber liver organoid tissue model)

176d [S164]

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