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K THENNARASI
KMC
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WHAT IS HUMAN CLONE ?HUMAN EMBRYO CLONE means
a human embryo that is a geneticcopy of another living or deadhuman but does not include a
human embryo created by thefertilisation of a human egg byhuman sperm.
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WHY HUMAN CLONING? To extract Embryonic Stem Cells
To cure diseases To develop Assisted
Reproductive techniques
To research stemcell ,
embryo development
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NORMAL FERTILISATION vs
CLONING
Method 1: Usual reproduction [in utero or in vitro]
Method 2: Somatic Cell Nuclear Transfer [SCNT]
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TYPES OF CLONINGEMBRYO CLONING
REPRODUCTIVE CLONING
THERAPEUTIC CLONING
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EMBRYO CLONING Produce monozygotic twins or triplets
From fertilised embryo
Remove one or more cells
Encourage to develop into one embryo
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Newly formed embryo containing DNA from somatic cell
cell division
implant
(Diploid )
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Removal of nucleus from adult cell
(Irving Weissman,2002)
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Transfer of nucleus to enucleatedoocyte
(Irving Weissman,2002)
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Nuclear transfer
Nuclear transfer embryo about to be activated
(Roslin Institute http://www.roslin.ac.uk/library/)
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REPRODUCTIVE CLONINGProduce a duplicate of existing
animal
Used to clone mammals
May produce genetic defects
Medical ethicist- immoral procedureand unsafe
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THERAPEUTIC CLONING DNA extracted from a human cell
Inserted into a womans ovum
Develop and produce stem cells Stem cells are removed from pre-embryo
Grown into specific organ
Transplanted into the patient
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THERAPEUTIC CLONING -
TARGETED DISEASES Diabetes mellitus (juvenile
form)
Spinal cord injury Parkinsons disease
Stroke
Amyotropic lateral sclerosis
Myocardial infarction
Congestive cardiac failure
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PROBLEMS WITH CLONINGUsually fails[ require many oocytes]
Faulty genetic reprogramming of cells
[Born clones have obvious or subtleabnormalities]
Probable issue of mitochondrial DNA (not
a perfect match some mitochondrial DNAcomes from mother or oocyte )
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STEM CELLSUnspecialised cells with 2 special
properties
*Ability to differentiate into othercell type
*Ability to self regeneration
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STEM CELLS- contdserves as
*Building blocks of various organs and
tissues in the body*Sort of repair system for the body
*Breakthrough in the REGENERATIVE
MEDICINE
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STEM CELL HISTORY1998 - Researchers first extract stem cells from humanembryos
1999 - First Successful human transplant of insulin-makingcells from cadavers
2001 - President Bush restricts federal funding for embryonicstem-cell research
2002 - Juvenile Diabetes Research Foundation Internationalcreates $20 million fund-raising effort to support stem-cell research
2003 - California okay stem cell research2004 - Harvard researchers grow stem cells from embryos
using private funding2004 - Ballot measure for $3 Billion bond for stem cells
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STEM CELLS
TOTIPOTENTCELLS
PLURIPOTENTCELLS
MULTIPOTENTCELLS
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TOTIPOTENT STEM CELLS UNLIMITED CAPABILITY
HUMAN CELLS DURING 1st FEW CELLDIVISIONS ALONE HAVE THIS POTENTIAL
FORM-EXTRA EMBRYONIC MEMBRANES
-TISSUES
-THE EMBRYO ITSELF-ALL POST EMBRYONIC TISSUES
-ORGANS
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PLURIPOTENT STEM CELLS
CAPABLE TO GIVE RISE TO MOST BUT NOT
ALL TISSUES OF AN ORGANISM
Eg. Inner cell mass
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Blastocyst Diagram
Princeton University
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Selection of embryonic stem cells
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Stem Cell Cultivation
University of Wisconsin-Madison
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STEM CELLS- SOURCES
ADULT
*Bone marrow
*MAPC (multipotent
adult progenitor cells)
*Hematopoietic cells
* CD34,CD133
*Umbilical cord, placenta
*menstrual blood
*Neural cells
*skin,liver
EMBRYO
*Primodial germ cells
* 5-9 wk oldembryo/fetus
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ADVANTAGES OF ADULT STEM
CELLSAUTOLOGOUS
NO TISSUE REJECTION
NO ETHICAL CONCERNS NO TERATOMAS
EASY TO OBTAIN [bonemarrow aspirate]
WIDELY AVAILABLE
GIVES RISE TO CELLS OF ALL GERM LAYERS
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DISADVANTAGES OF ADULT STEM
CELLS
Not as PLURIPOTENT as
EMBRYONIC STEM CELLS
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PROBLEMS WITH EMBRYONIC
STEM CELLS
REJECTION FORM TERATOMAS
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CORD BLOOD STEM CELLSContain all elements of blood- RBC, WBC, PLATELET, PLASMA
Increasingly used alternative to BM
Used to restore functional deficiencies
of several genetic and metabolicdiseases
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STEM CELL BANKINGSources cord blood ,- 60 to 80ml ,
umbilical cord 12cm
menstrual blood
Transported to CRYOCELL
Storage -196 degrees celcius for 21 yearsin liquid nitrogen
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MENSTRUAL BLOOD STEM CELLS High regenerative potential[6-7mm/ month] Rich source of self renewing cells Harvesting noninvasive, painless ,safe
MESENCHYMAL STEM CELLS under clinicaltrials
Therapeutic applications-copd-cardiac arrythmias-cns disorders-critical limb injuries-graft vs host disease
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TAKE HOME POINTS THERAPEUTIC CLONING RESEARCH gives
*better understanding of human developmentand stem cell research
* develop new treatment of presently incurablediseases in particular genetic ,serious injury
*enables perfectly match transplant tissue
*practical application not expected for atleast 10years
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THANK YOU