Clinical use of spermat Clinical use of spermat id id ค ค Introduction Introduction ค ค Spermiogenesis block Spermiogenesis block ค ค Prognostic factors of spermatid Prognostic factors of spermatid conception conception ค ค Potential health hazards Potential health hazards ค ค Practical recommendation Practical recommendation
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Clinical use of spermatid ค Introduction ค Spermiogenesis block ค Prognostic factors of spermatid conception ค Potential health hazards ค Practical recommendation.
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Clinical use of spermatidClinical use of spermatid
คค IntroductionIntroduction
คค Spermiogenesis blockSpermiogenesis block
คค Prognostic factors of spermatid conceptionPrognostic factors of spermatid conception
คค Potential health hazardsPotential health hazards
คค 3 babies from ROSI3 babies from ROSIคค 9 babies from ELSI9 babies from ELSI
Spermatid conceptionsSpermatid conceptions
คค Highly variable and unpredictable Highly variable and unpredictable outcomes of spermatid conceptionoutcomes of spermatid conceptionคค All nuclear and cytoplasmic All nuclear and cytoplasmic modification may not fully modification may not fully development development คค Possible health hazards Possible health hazards
Spermatid classificationSpermatid classification
คค The round S. (Sa, Sb1)The round S. (Sa, Sb1)
คค The elongating S. (Sb2)The elongating S. (Sb2)
คค The elongated S. (Sc,Sd1)The elongated S. (Sc,Sd1)
คค The late elongated S.(Sd2) or The late elongated S.(Sd2) or
คค Ejaculated spermatids from Ejaculated spermatids from spermiogenesis arrest patientsspermiogenesis arrest patientsคค- Not related to serum FSH- Not related to serum FSH
- Abnormal relationship - Abnormal relationship between spermatids and between spermatids and Sertoli cellsSertoli cells Priming Sertoli cells with FSHPriming Sertoli cells with FSH
คค Round spermatids in ejaculate and Round spermatids in ejaculate and testicular biopsy from patients testicular biopsy from patients undergoing a spermatid conception undergoing a spermatid conception attempt. attempt.
- 1/2 - Round spermatid- 1/2 - Round spermatid- 1/2 - Elongated spermatid or no - 1/2 - Elongated spermatid or no
spematidspematid
EjaculateEjaculate
++++--
BiopsyBiopsy
++--++
No (%) of patientsNo (%) of patients
30(45)30(45)27(41)27(41)9(14)9(14)
(Tesarik J., et al. Human Reproduction 1998 ;13 (Tesarik J., et al. Human Reproduction 1998 ;13 Suppl 3 : 89-107)Suppl 3 : 89-107)
66 patients with complete spermiogenesis failure66 patients with complete spermiogenesis failure
- A - A few foci produced ROSfew foci produced ROS- ROS production has a cyclic - ROS production has a cyclic character character - Prematurely released - Prematurely released spermatocytes develop into spermatocytes develop into spermatids spermatids - Spermatids from biopsy have a - Spermatids from biopsy have a higher biologic quality, fertilizing higher biologic quality, fertilizing abilityability
Prognostic factors of spermatid Prognostic factors of spermatid conceptionconception
คค Complete and incomplete spermiogenesis Complete and incomplete spermiogenesis
คค 2 Ongoing clinical pregnancies - from 3 2 Ongoing clinical pregnancies - from 3
ELSIELSI
คค 4 Biochemical pregnancies - from ROSI4 Biochemical pregnancies - from ROSI
คค Incomplete spermiogenesis failure is Incomplete spermiogenesis failure is
better better
คค Fertilization and cleavage rates in Fertilization and cleavage rates in complete spermiogenesis failurecomplete spermiogenesis failureคค Factors at implantation and Factors at implantation and post-implantation period.post-implantation period.
Genetic anomalies.Genetic anomalies.คค Human spermatogenic disorderHuman spermatogenic disorder
คค Mature sperm cell : 1 centriole Mature sperm cell : 1 centriole associated with MTOCassociated with MTOCคค Distal centriole of spermatidDistal centriole of spermatid
- May associate with MTOC- May associate with MTOC- Multipolar mitotic spindle- Multipolar mitotic spindle- Chromosomal abnormalities- Chromosomal abnormalities
Oocyte activating factorOocyte activating factor
คค Sperm-induced activation of human Sperm-induced activation of human
oocytes. oocytes.
- Ca- Ca2+2+ spiking or oscillations spiking or oscillations
- Dependent on the oocyte-activating - Dependent on the oocyte-activating
factor in cytoplasm of sperm factor in cytoplasm of sperm
- Ca2+ oscillation occurs in ROSI but - Ca2+ oscillation occurs in ROSI but not after the injection of 1not after the injection of 1คคor 2or 2คคspermatocyte or PMN spermatocyte or PMN
- Spermatids from men who produced - Spermatids from men who produced normal mature sperm normal mature sperm
Oocyte activating factor (Cont.)Oocyte activating factor (Cont.)คค Tesarik J., et al. (Hum Repecd 1998; B Tesarik J., et al. (Hum Repecd 1998; B
supple 3:89-107)supple 3:89-107)
- Spermatids from men with complete - Spermatids from men with complete
spermiogenesis failurespermiogenesis failure
- 6 in 36 oocytes (17%) showed Ca- 6 in 36 oocytes (17%) showed Ca2+2+ oscillation at 30-60 min oscillation at 30-60 min
- 2 in 36 oocytes (6%) showed - 2 in 36 oocytes (6%) showed
CaCa2+2+oscillation at 2-3hr.oscillation at 2-3hr.