Citric Acid Cycle What is it? Series of rxns that oxidize acetyl CoA to 2CO 2 in a manner that conserves the liberated free energy for ATP production Breakdown products of glc, fats and amino acids are all oxidized via the cycle Intermediates also a source for many biosynthetic pathways - “amphibolic” which means serves in both catabolic and anabolic processes Also called “TCA cycle” - tricarboxylic acid cycle or “Krebs cycle”
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Citric Acid Cycle - Creighton University - Chemistrychemistry.creighton.edu/~jksoukup/lec18TCASTUD.pdf · Citric Acid Cycle Regulation Rate of cycle is determined by: 1. Availability
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Citric Acid Cycle
What is it?
Series of rxns that oxidize acetyl CoA to 2CO2 in a manner thatconserves the liberated free energy for ATP production
Breakdown products of glc, fats and amino acids are all oxidized viathe cycle
Intermediates also a source for many biosynthetic pathways -“amphibolic” which means serves in both catabolic and anabolicprocesses
Also called “TCA cycle” - tricarboxylic acid cycle or “Krebs cycle”
Citric Acid Cycle
Cellular respiration
Citric Acid CyclePyruvate dehydrogenase complex
3 mitochondrial euk enzymes5 coenzymes
Citric Acid CyclePyruvate dehydrogenase complex5 coenzymes - Coenzyme A (pantothenate)Reactive thiol is an acyl carrier
Citric Acid CyclePyruvate dehydrogenase complex5 coenzymes - NAD+ (niacin)Involved in redox, electron carrier
Citric Acid CyclePyruvate dehydrogenase complex5 coenzymes - FAD (riboflavin, vitamin B2)Involved in redox, electron carrier
Citric Acid CyclePyruvate dehydrogenase complex5 coenzymes - TPP (thiamine, vitamin B1)Helps in cleavage of bonds next to carbonyl
Citric Acid CyclePyruvate dehydrogenase complex5 coenzymes - lipoateReversible oxidation at thiols, serves as electron carrier and acylcarrier
RegulationPyruvate: substrate for complexADP: indicates low energy charge NADH, acetyl CoA: endproducts of complexCa2+: certain hormones (insulin) raise [Ca2+]
Citric Acid CycleEvery turn of cycle:1. Joining an acetyl group to oxaloacetate2. Oxidizing 2 carbons off as CO2, leaving succinate3. Converting succinate back to oxaloacetate4. Producing 1 GTP and reduced cofactors
Citric Acid Cycle
Net reaction:AcetylCoA + 3NAD+ + FAD + GDP + Pi + 2H2O → 2CO2 + CoA + 3NADH + FADH2 +GTP + 2H+
Citric Acid CycleGoal:capture energy from acetate → 2CO21. One GTP made: equivalent to 1 ATP2. Lots of reduced cofactors, reducing power can be converted to ATP
Citric Acid CycleRegulationRate of cycle is determined by:1. Availability of substrates (acetyl CoA, NAD+, FAD)2. Inhibition by accumulating products3. Allosteric feedback inhibition of enzymes
NAD+ and FAD are regeneratedby oxidative phosphorylation,which needs O2
Citric Acid CycleReaction 5 - Succinyl-CoA synthetaseHydrolysis of thioester bond energizes formation of GTPSynthetase indicates participation of nucleoside triphosphateReversible
Citric Acid CycleReaction 6 - Succinate dehydrogenaseOxidation of succinate to fumarateEnzyme bound to inner mito membrane, only membrane-bound enzReversible
Malonate (analog of succinate) is a competitive inhibitor of enz,blocks activity of cycle
Citric Acid CycleReaction 7 - FumaraseHydration of fumarate to L-malateReversible
Substrate
Product
Citric Acid CycleReaction 8 - Malate dehydrogenaseOxidation of malate to oxaloacetateNAD+ linked to enzymeHigh +ΔG˚, pulled to products by continual removal of oxaloacetate