Chymotrypsin • Chymotrypsin is one of the serine proteases. • Chymotrypsin is selective for peptide bonds with aromatic or large hydrophobic side chains, such as Tyr, Trp, Phe and Met, which are on the carboxyl side of this bond. It can also catalyze the hydrolysis of easter bond. • The main catalytic driving force for Chymotrypsin is the set of three amino acid known as catalytic triad. This catalytic pocket is found in the whole serine protease family.
Chymotrypsin Chymotrypsin is one of the serine proteases. Chymotrypsin is selective for peptide bonds with aromatic or large hydrophobic side chains, such.
Dec 19, 2015
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Chymotrypsin
• Chymotrypsin is one of the serine proteases.
• Chymotrypsin is selective for peptide bonds with aromatic or large hydrophobic side chains, such as Tyr, Trp, Phe and Met, which are on the carboxyl side of this bond. It can also catalyze the hydrolysis of easter bond.
• The main catalytic driving force for Chymotrypsin is the set of three amino acid known as catalytic triad. This catalytic pocket is found in the whole serine protease family.
Properties of an Active Site
• A shape that fits a specific substrate or substrates only
• Side chains that attract the enzyme particular substrate
• Side chains specifically positioned to speed the reaction
The Catalytic Triad
Chymotrypsin Protein HydrolysisStage #1
Chymotrypsin Protein HydrolysisStage #2
Chymotrypsin Protein HydrolysisStage #3
Chymotrypsin Protein HydrolysisStage #4
Chymotrypsin Protein HydrolysisStage #5
Chymotrypsin Protein HydrolysisStage #6
Transition State Stabilization
Chymotrypsin Kinetics
The initial "burst" in chymotrypsin-catalysed hydrolysis of the p-nitrophenyl acetate
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