Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.

Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy

protein productionBy Xin Li

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• To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant.

Purposes

• To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant.

• To produce cheaper soydiesel, more soy protein, and less expensive ethanol from the soybean plants.

Purposes

Modeling Soybean

Arabidopsis(Arabidopsis thaliana)

Soybean(Glycine max)

Modeling Soybean

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Arabidopsis(Arabidopsis thaliana)

Soybean(Glycine max)

Modeling Soybean

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Arabidopsis(Arabidopsis thaliana)

Soybean(Glycine max)

Modeling Soybean

•Both are oilseed plants

•Arabidopsis has a mapped genome

•Insertion-induced Arabidopsis mutants are av

•ailable commercially

Modeling Soybean

•Both are oilseed plants

•Arabidopsis has a mapped genome

•Insertion-induced Arabidopsis mutants are av

•ailable commercially

Modeling Soybean

•Both are oilseed plants

•Arabidopsis has a mapped genome

•Insertion-induced Arabidopsis mutants are av

•ailable commercially

•Insertion-induced Arabidopsis mutants are available commercially

Insertion-Induced Mutation

T-DNA

Gene

Insertion-Induced Mutation

Insertion-Induced Mutation

Insertion-Induced Mutation

Insertion-Induced Mutation

Insertion-Induced Mutation

Wild-type

Hypersensitive mutant

Insensitive mutant

Methods

• Grew 300 seedlings of each of 58 different mutants and wild-type in:

Methods

• Grew 300 seedlings of each of 58 different mutants and wild-type in:

• 6% glucose

Methods

• Grew 300 seedlings of each of 58 different mutants and wild-type in:

• 6% glucose

• 6% sucrose

Methods

• Grew 300 seedlings of each of 58 different mutants and wild-type in:

• 6% glucose

• 6% sucrose

• Measured root length of seedlings grown in glucose (Gibson, 2005)

Methods

• Grew 300 seedlings of each of 58 different mutants and wild-type in:

• 6% glucose

• 6% sucrose

• Measured root length of seedlings grown in glucose (Gibson, 2005)

• Use spectrophotometry to determine anthocyanin levels of seedlings grown in sucrose (Nacry,1998)

Figure 1. Root lengths after 11 days: Results for the mutants shown in Figures 1 and 2 except for

SALK_113292 are representative of all other 57 Arabidopsis mutants tested

0.000.200.400.600.801.001.201.401.601.802.00

SALK_143055SALK_083114SALK_113292SALK_080380SALK_070394SALK_008072

Col-O

Root lengths

Results

Figure 1. Root lengths after 11 days: Results for the mutants shown in Figures 1 and 2 except for

SALK_113292 are representative of all other 57 Arabidopsis mutants tested

0.000.200.400.600.801.001.201.401.601.802.00

SALK_143055SALK_083114SALK_113292SALK_080380SALK_070394SALK_008072

Col-O

Root lengths

Results

Results

Results

ResultsNeff and Chory (1998)

Results

Conclusion

• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.

Conclusion

• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.

• The mutant’s genotype is SALK_113292.

Conclusion

• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.

• The mutant’s genotype is SALK_113292.

• The disabled gene in mutant SALK_113292 is at1g06230.

Conclusion

• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.

• The mutant’s genotype is SALK_113292.

• The disabled gene in mutant SALK_113292 is at1g06230.

• The gene contains a bromodomain protein

Future Work

• Grow SALK_113292 in 1% glucose and 1% sucrose

Future Work

• Grow SALK_113292 in 1% glucose and 1% sucrose

• Grow SALK_113292 in 1% sorbitol and 6% sorbitol

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Image from: htto://www.food-info.net

Acknowledgement

• Dr. Sue Gibson

• Dr. Chunyao Li

• Ms. Lois Fruen

• Ms. Chelen Johnson

• Dr. Jacob Miller

• Ms. Virginia Amundson

• Breck Advanced Team Research

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http://www.cbs.umn.edu/plantbio/faculty/GibsonSue/

Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy

protein productionBy Xin Li

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