Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris cv. Blauhilde Brandon Lew March 24, 2007.

Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris

cv. Blauhilde

Brandon Lew

March 24, 2007

Lab Work - Hamline University

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Goal: To identify pigments that cause the purple coloration of pod coats in Phaseolus vulgaris cv. Blauhilde

Hypothesis: The purple color is caused by anthocyanins

Phaseolus vulgaris

• Kidney, black, navy, and snap beans

• Anthocyanins found in:– Seed coat of kidney beans

Choung et al. 2003

– Seed coat of black beansTakeoka et al. 2005

• Neither pod coats, not cultivar Blauhilde studied

Cultivar Blauhilde

• Pod coats purple• Purple disappears when cooked

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Anthocyanins

• Pigments

• Anti-carcinogenicKatsube et al. 2003

• Anti-oxidativeTamura and Yamagami, 1994

Colorful,Good for health

Methods

High-Performance Liquid Chromatography (HPLC)

Small substances

Large substances

HPLC of Extract

Isoabsorbance of Extract

HPLC after Purification

Isoabsorbance after Purification

Mass Spectroscopy

Detector

FragmentsMoleculeMagnets

e-

Mass Spectrum of Extract

Identified Molecule

OOH

OHOH

OH O

OH

OH

OHOH

O+

OH

O

O

OCH3

OH

OCH3

Malvidin 3,5-diglucoside

Conclusion

• Identified anthocyanin as malvidin 3,5-diglucoside

• 86% of total anthocyanin content

Future Work

• Genetically engineer different anthocyanins– Keeps health benefits– Different colors

Acknowledgements

• Dr. John Matachek• Hamline University Chemistry Dept.• Ms. Fruen• Ms. Richter• Team Research

Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris

cv. Blauhilde

Brandon Lew

March 24, 2007