Announcements • UV/VIS printer issues during Chapter 1 – TFs should have emailed a PDF of UV/VIS data • Chapter 2 labs begin Wednesday (9/20) • Chapter 2 pre-lab write-ups are due at the beginning and end of lab • Chapter 1 post-lab write-ups are also due at the beginning of lab • Quiz at the end of discussion today
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Announcements• UV/VIS printer issues during Chapter 1 – TFs should
have emailed a PDF of UV/VIS data
• Chapter 2 labs begin Wednesday (9/20)
• Chapter 2 pre-lab write-ups are due at the beginning and end of lab
• Chapter 1 post-lab write-ups are also due at the beginning of lab
• Quiz at the end of discussion today
Example of a proper figure legend
Absorbance Spectrum
Figure 3. Absorbance spectra of different macromolecules. Absorbance spectra for gelatin (red), BSA (blue), and yeast RNA (green) are ploted between 230 and 360 nm. Between this wavelength range, BSA displays an absorbance peak at 277 nm…
Chapter 2: Buffers and Titrations
Procedures• Calibrate pH meter and measure pH of different solutions• Titrate histidine solution and deionized water to obtain a
corrected histidine titration curve• Perform a pH stat experiment (hydrolyze BSA with
trypsin) to calculate the number of Lys and Arg residues in BSA
pH Meter● Measures the difference in electric
potential between a pH electrode and a reference electrode
● Difference in electric potential related to acidity (pH) of the solution.
● Glass‐electrode sensitive to hydrogen ions
● Electrode somewhat sensitive to other alkali metals (Na+ vs K+)
● Complete system contains:● Electrometer – 5● Reference electrode – 6● Solution to be measured – 1,4● pH glass electrode – 2,3