Chanleakhena Phoeung, Aung Naing Myo, Stéphane Bayen, Boon Huan Tan, Nicolas Steenkeste, Khov Meas, Puy Lim, Monidarin Chou, Barry Kelly Symposium on Biodiversity.

DETECTION OF PESTICIDES IN FISH FROM TONLE SAP LAKE

CAMBODIAChanleakhena Phoeung, Aung Naing Myo, Stéphane Bayen, Boon Huan Tan, Nicolas

Steenkeste, Khov Meas, Puy Lim, Monidarin Chou, Barry Kelly

Symposium on Biodiversity & Health Symposium17-18th November 2014

Outline• Introduction

• Exposure to Pesticides

• Objective and Method of study

• Results of study

• Conclusion

• Recommendations

Source : Nile River Awareness Kit "Physical and Chemical Characteristics of Water Quality."

Introduction

Problems bioaccumulation and Biomagnification

Detection the presence of frequently used pesticides in

fish collected from Tonle Sap lake in Cambodia

Target compounds : 65 POPs– Organochlorines (OCPs)– Polychlorinate biphenyls (PCBs)– Bis-(p-chlorophenyl) sulfone (BCPS)– ……

Objective of Study

• Sampling sites : Siem Reap, Pursat, K. Thom, K. Chhnang

• 76 fishes collected

• 36 samples analyzed

-12 (12 species)from Siem Reap

-15 (12 species)from Pursat

- 4 ( 4 species) from K. Thom

- 5 (5 species) from K. Chhnang

Study sites and sample collection

TS1

TS2

TS3

TS4

Chong Kneas

Phat SandayKamPong Luong

Chnok Trou

Gel permeation chromatography (GPC) with organic solvents followed by Florisil Clean-up

Samples processing

8

Injector : splitless mode

Gaz : He

Column : DB-5MS

60 m X 0.25 mm X 0,10 µm

T˚ column : 80˚C

Inj. Vol : 3 ml/min, T˚ inj : 250˚C

Flow rate : 1,2 ml/min

Presure : 155,7 kPa1min

80˚C

160˚C

20˚C/min

200 ˚C

4 ˚C/min

250 ˚C

2 ˚C/min

20 ˚C/min 10min

Gradient

300˚C

GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) : Condition

Samples Analysis

RESULTS

10

Samples characteristic

ត� កែ�ស ច�កែ�ង

ប�មា ��� ឆលា� ង�ន�ងប�ង ឆកោឆលា� � �ញចះ��� ឆកោ�

ឆ�មា�

��យ សនតា�យ �� ��ឡ

ង� សលា� តអាចម!���

Total

TS1 4 6 3 1 3 3 3 3 1 2 1 1 0 0 0 0 31

TS2 0 1 0 1 0 0 0 3 1 0 0 0 10 3 3 0 22

TS3 0 0 1 1 3 0 0 0 1 0 0 0 0 1 0 10 17

TS4 1 1 1 1 0 1 0 0 1 0 0 0 0 0 0 0 6

Total 5 8 5 4 6 4 3 6 4 2 1 1 10 4 3 10 76

Main POPs detectedCompound name Concentration Permissible limit

p,p’- DDT 0.00 to 3.70 ng/g 5ppm

PCBs 0.00 to 9.99 ng/g 2ppm

BCPS 0.00 to 0.86 ng/g 10ppm

FAO J Toxicol Environ Health A. 1999 Jun 11;57(3):185-98.

194199

200202

203204

205209

212215

216217

244247

249251

252255

256257

260264

274303

306314

315316

317330

333336

338349

0

0.5

1

1.5

2

2.5

3

3.5

43.7

2.23

0.21

Result of DDT

Samples

Concentration (ng/g)

194199

200202

203204

205209

212215

216217

244247

249251

252255

256257

260264

274303

306314

315316

317330

333336

338349

0

0.5

1

1.5

2

2.5

3

3.5

4

0.3 0.29

1.06

0.37

3.69

Result of PCBs

Samples

Mean of Concentration(ng/g)

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

0.67

0.86

0.75

0.03

0.85

0.71

Result of BCPSConcentration(ng/g)

Samples

Siem Reap Pursat K. Thom K. Chhnang0

2

4

6

8

10

12

3.7

00.86

9.99Chart Title

p,p'- DDTPCBsBCPS

Max Con-centration

ng/g

POPs detected in different sites

Study sites

Conclusion• Trace of dangerous compounds are detected in Tonle Sap fish

• Predominant compounds are p,p’-DDT, PCBs and BCPS.

• Concentration of each compound is variable from one site to another

• These compounds are bioaccumulated and biomagnified it may affect

to the people.

• Limit use of dangerous pesticides by educating users about its hazard

• Monitor residue frequently of pesticides in ecology system

Acknowledgements

THANK YOU