9/7/2016 1 Microbial metabolism Chapter 5 BIO 220 Metabolism • Sum of all the chemical reactions occurring in an organism • Metabolism = Catabolism + Anabolism Fig. 5.1 Collision Theory • In order for chemical reactions to take place, atoms/ions/molecules must collide with each other • The energy transferred during these collisions allows for the formation or the break down of chemical bonds Enzymes • Enzymes increase reaction rate by increasing the probability that substrates will interact in an orientation necessary for product formation • They lower the Energy of Activation of the reaction (decrease the randomness of substrate interactions)
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Ch 5 - Microbial metabolism · Microbial metabolism Chapter 5 BIO 220 Metabolism • Sum of all the chemical reactions occurring in an organism • Metabolism = Catabolism + Anabolism
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9/7/2016
1
Microbial metabolism
Chapter 5
BIO 220
Metabolism
• Sum of all the chemical reactions occurring in
an organism
• Metabolism = Catabolism + Anabolism
Fig. 5.1
Collision Theory
• In order for chemical reactions to take place,
atoms/ions/molecules must collide with each
other
• The energy transferred during these collisions
allows for the formation or the break down of
chemical bonds
Enzymes
• Enzymes increase reaction rate by increasing
the probability that substrates will interact in
an orientation necessary for product
formation
• They lower the Energy of Activation of the
reaction (decrease the randomness of
substrate interactions)
9/7/2016
2
Activation energy
Fig. 5.2
Enzyme action
Fig. 5.3
Enzyme Characteristics
1. Biological catalysts
– Can process substrates very efficiently
– Turnover number
2. Induced fit vs. lock and key
3. Usually proteins
4. Substrate smaller than enzyme
5. Specificity (affinity)
6. Naming
– End in -ase
– Based on type of chemical reactions they catalyze