Page 1
The Cell
Observed by Light and Electron Microscopy
Page 2
Overview of the Cellular Organelles
Page 3
Light Microscopy – Hematoxylin & Eosin (H&E) Stain
Basophilic (blue) Nuclei
Eosinophilic (red)
cytoplasm
Page 4
Colorized low mag TEM of a cell
Red = Mitos
Blue = Ribos
Green = RER
Page 5
Colorized TEM of a cell nucleus
Arrow = Nucleolus
Red = Mitos
Green = RER
Page 6
Nuclear Structures
Page 7
Low mag TEM of a nuclei
Page 8
Nuclear pores (arrows) Nuclear pores (en face)
Page 9
Colorized TEM of nuclear pores (blue) en face
Page 10
High mag TEM of a nucleolus
Pars fibrosa/granulosa (dense black) and
fibrillar center (arrow)
Page 11
TEM of interphase nuclei and a mitotic cell with condensed chromosomes (arrow)
Page 12
Cytoplasmic Structures
Ribosomes, RER and Glogi
Page 13
Ribosomes by light microscopy = basophilic cytoplasm
Page 14
TEMs of free ribosomes – 25 nm granules – in clusters called polysomes
Page 15
Colorized TEM of polysomes (yellow highlighted)
Page 16
Low mag TEM of rough endoplasmic reticulum (RER)
Page 17
Colorized higher mag TEM of RER (green)
Page 18
TEM of RER – arrows indicate cisternae
Page 19
Low mag TEM of RER and Golgi (Go)
Page 20
Colorized TEM of a Golgi system
Page 21
TEM of a Golgi system
Page 22
TEM of lysosomes (arrows) & a residual body
Residual Body
Page 23
Cytoplasmic inclusions by Light Microscopy
Basophilic granules (top picture)
Eosinophilic granules (bottom picture)
Page 25
Colorized TEM of Mitochrondria (red)
Page 26
TEMs of Mitochrondrial shapes
Page 27
TEM of a Mitochrondrion
Arrows = cristae
Page 29
Light microscopy of mitotic spindles = Microtubules
Page 30
Colorized TEM of Cilia in cross section showing microtubules
Page 31
Immunofluorescence of Microtubules in cultured cells (green)
Page 32
TEM of Microfilaments (arrows)
Page 33
Immunofluorescence of microfilament stress fibers in cultured cells
Page 35
High mag TEM of two cell membranes (unit membrane)
Page 36
Colorized TEM of two cell membranes (unit membrane)
Page 37
TEMs of membrane specializations
Top 4 = microvilli
Middle = Stereo cilia
Bottom = Cilia
Page 38
TEM of Cilia (C) with microtubular cores forming from basal
bodies (BB)
Page 39
High mag TEM of Cilia with
microtubular cores (Fil) with the basal body (FiC) at top of
image