cell disruption protein extraction enzyme release continuous processing DNA manipulation single cell isolation tissue disruption selective breakage Constant Systems Research Models Cell Disruption Solutions Cell Disruption it’s in our Genes Taking your disruption out of Cell Disruption ISO 9001:2000 Registered FS67550 www. constantsystems.com Rev 0
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cell disruption
protein extraction
enzyme release
continuous processing
DNA manipulation
single cell isolation
tissue disruption
selective breakage
Constant Systems Research Models
Cell Disruption Solutions
Cell Disruption it’s in our Genes
Taking your disruption out of Cell Disruption
ISO 9001:2000 RegisteredFS67550
www.constantsystems.com
Rev 0
Principles of Operationof
Constant Cell Disruption Systems are based on the use of high pressure to force a sample through a small fixed orifice at high speed under controlled, contained and repeatable conditions.
As the high pressure piston descends, sample is introduced into the high pressure cylinder.
1.
The high pressure piston then accelerates the sample through the fixed orifice at high velocity. The controlled acceleration causes the cells to rupture.
2.
When the jet containing disrupted cells hits the target, it is spread radially across the cooled heat exchange surface. Product then flows from the disruption chamber for collection. The hydraulics are recharged and the cycle continues.
3.
Reproducible ResultsConstant Cell Disruption Systems give reproducible and superior results because control of the disruption pressure is achieved by the hydraulic control system.
The disruption pressure cycle is shown adjacent. This has been created by analysing and recording the hydraulic pressure in the operating cylinder, which is related to the disruption pressure by the ratio of the piston areas.
Contained ProcessThe disruption head, high pressure cylinder and cooling system have been designed with containment and safety in mind so that the volume of high pressure product is kept to minimum at all times. The integrity of the containment system has been tested by the Biosafety Testing Station at the Centre for Applied Microbiology Research, UK. “ ...a high level of bio-containment.” “No evidence of emission from the equipment in any test.”
2
Graph showing a typical controlled pressure cycle in the cell disrupter
Disruptionstroke
Returnstroke
Recharging period for hydraulics
30
20
10
40
30
20
10Pre
ssur
e (b
ar x
100
) Pressure (psi x 1000)
2 4 6 8 10 12 14Time (second)
Cell Disruption Solutions
Specifications shown over leaf
Specifications shown overleaf
The One Shot model is the ideal research tool for small processing. It can process solid samples, cell paste and suspended samples quickly and efficiently. Work and results can be scaled up throughout the range.
Temperature Control: Disruption head is surrounded by a unique cooling jacket . Bath circulator or chiller is recommended.
Cell Disruption Solutions
The B & C Series are the ultimate industry cell disrupters, with the power and processing ability to consistently disrupt the materials that you require.
Validation: Industry standard validations available on request
690
785
*B Series Bench Top model shown
above and B series Cabinet model
shown on the reverse
Constant Cell Disrupter Systems are extremely versatile and have been used for the following range of application:
Cell Disruption, Protein & DNA Extraction, Enzyme Release, DNA Manipulation, Selective Breakage, Single Cell Isolation, Tissue Disruption
Individual Organisms and samples processed by Constant Cell Disrupter Systems are too numerous to mention them all, however the following lists examples:
Algae Disruption of anabaena variableAnimal Cells Selective breakage of chicken spermBacteria Good enzyme activity from recombinant e.coli. High protein recovery from mycobacterium smegmatis
Fungi High specific activity of enzyme from aspergillus nidulans. 99% protein release(inc Yeasts) from saccharomyces cerevisiae. 99% Recombinant protein release from pichia
pastoris
Mammalian Disruption of cellular and intracellular plasma membranes from horse liver IsolationCells and Tissue of viable single cells from calf spleen and lymph node
Parasites Breakage of cytomegalovirus in nucleus of human cells
Plant Cells Pectin extraction from banana fruit. Strawberry leaf DNA extraction& Tissue
Viruses Isolation of membrane antigens from toxoplasma gondi. Separation of nucleus and membrane in amniotic cells
Environmental Extraction of DNA from soil bacteriaSamples
E.coli Disruption & Guidance List
Constant Systems Cell Disrupters experience with E.coli is vast and breakage depends on the cell culture conditions, length of growth cycle an dpressure utilised.We state the following as a guide to users for pressure compared to breakage with E.coli:
Release of DNA – Below 15,000 psi (1000 Bar)
Cytoplasmic Protein – 15,000 psi – 20,000 psi (1350 Bar)