CECT 516 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Other culture collections: ATCC 8739; CCRC 11634; CCTM La 2194; CCUG 10979; CIP 53.126; DSM 1576; Gunsalus strain Crookes; IFO 3972; IMET 11121; LMG 8063; NCFB 904; NCIMB 8545; PCM 2561; VTT E-76039; WDCM 00012 History: CECT, 1982 < NCIMB, 1953 < ATCC < I. C. Gunsalus < G. C. Crooks Isolation source: Human faeces Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214 Risk group: 2 Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h CHARACTERIZATION Miniaturized biochemical test API 20E Growth on selective/differential culture media CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results. ENDO AGAR Red colonies, red medium, golden sheen TERGITOL AGAR Yellow colonies with yellow zone. Sometimes with rust coloured centre + - + - - - - - + * - + + - + + - + * + - 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 ONPG ADH LDC ODC CIT H 2 S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX 5 0 4 4/5 5 5 2/3 Identification: 94.7-99.9% ID Escherichia coli C E R T I F I E D CERTIFICADA 20121024 TBX (Tryptone Bile X- glucuronide) AGAR Blue-green colonies Page 1 of 2 *This result may vary