PH0500004 PHILIPPINE NUCLEAR RESEARCH INSTITUTE - SClENTiFiC LIBRARY AND DOCUMENTATION CENTER POLYTECHNIC UNIVERSITY OF TEE PHILIPPINES Sta. Mesa, Manila CC MPARATIVE STUDY ON THE PEROXIDASE ACTIVITY FROM TH^JLOATS OF Caulerpa lentillifera (GRAPES SEAWEEDS), ROOTS OF Tamarindus indica (TAMARIND), Eichhornia crassipes (WATER HYACINTH) AND Dracaena surculosa (SPOTTED DRACAENA) An UNDERGRADUATE THESIS Presented to the Faculty of the Department of Natural Sciences College of Science In Partial Fulfillment of the Requirements for the Degree of BACHELOR OF SCIENCE IN CHEMISTRY By Maan Dyann N. Ber-6sil Cherrie Joy C. Magtibay February 2005
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CC MPARATIVE STUDY ON THE PEROXIDASE ACTIVITY …Determination of peroxidase at 510 nm of the four extracts indicated that, spotted dracaena gave the highest peroxidase activity with
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PH0500004
PHILIPPINE NUCLEAR RESEARCH INSTITUTE -SClENTiFiC LIBRARY AND DOCUMENTATION CENTER
POLYTECHNIC UNIVERSITY OF TEE PHILIPPINESSta. Mesa, Manila
CC MPARATIVE STUDY ON THE PEROXIDASE ACTIVITY FROMTH^JLOATS OF Caulerpa lentillifera (GRAPES SEAWEEDS),
ROOTS OF Tamarindus indica (TAMARIND), Eichhorniacrassipes (WATER HYACINTH) AND Dracaena
surculosa (SPOTTED DRACAENA)
An UNDERGRADUATE THESISPresented to the Faculty of theDepartment of Natural Sciences
College of Science
In Partial Fulfillment of theRequirements for the Degree of
BACHELOR OF SCIENCE IN CHEMISTRY
ByMaan Dyann N. Ber-6silCherrie Joy C. Magtibay
February 2005
POLYTECHNIC UNIVERSITY OF THE PHILIPPINESSta. Mesa, Manila
APPROVAL SHEET
This is to certify that this thesis entitled "COMPARATIVE STUDY ON THEPEROODASE ACTIVITY FROM THE FLOATS OF Caiderpa lentillifera (GRAPESSEAWEEDS), ROOTS OF Tamarindus indica (TAMARIND), Eichhornia crassipes(WATER HYACINTH) AND Dracaena surculosa (SPOTTED DRACAENA)" preparedand si bmitted by Maan Dyann N. Berosil and Cherrie Joy C. Magtibay to fulfill therequirements for the degree of Bachelor of Science in Chemistry has been examined bythe Committee on Oral Examination, successfully defended and approved with a grade ofPASSKD on February 21, 2005.
ai>i ?Abigail P. Cid
Adviser
Lorna T.(yEnerva, Ed. D.Examiner
IUJoy]$. Htofilefia, Ph. D. Erigr. Liduvina C.
Examiner Examiner
The Department of Natural Sciences endorses acceptance of this thesis as a partialfulfill snent of the requirements for the degree of Bachelor of Science in Chemistry.
laida R. parmiento, Ed. D.Chaij", Department of Natural Sciences
College of Science
This thesis is hereby officially accepted as partial fulfillment of the requirementsfor .he degree of Bachelor of Science in Chemistry.
Normita B: Geirospe, Ed. D.Dean, College of Science
l i
ACKNOWLEDGEMENT
We would like to give credit to Christian Jay Cambiador and Ms.
Maria Celestina Honrado that have a big part in our thesis. Christian Jay for
sharing his God-given talent with us from the very beginning our thesis and for
jeing a.k.a "student thesis adviser. Ma'am Cel for being appioachable that made
us feel comfortable to ask whenever we had problems during our laboratory
experiment, for guiding us throughout our thesis even though she was just
assigned to supervised us and for staying with us even it is already an overtime.
We would also like to thanks the PNR1 employees and staff, for
imdoubtfully letting use their facilities and resources.
To Ms. Elvira Z. Sombrito for giving her recommendations for us to
avail a laboratory room.
To Ms. Merrian Tangonan or Ma'am Yen for assisting us at times
Ma'am Cel is not around.
To Ms. Xena for sparing us some of the reagents we needed.
To Sir Chito for trusting us to used almost everyday the UV-Vis
spectrophotometer.
To Biboy for providing and lending us the apparatus we needed.
To Kuya Glenn for cutting some parts of the roots of tamarind tree.
And to all the chemists and biologists in the ARD building for helping us
iround.
To our frrnds namely Arvie, Jheng, Nico, Bamba, Marco, and Mamu
for providing us some of the reagents we needed.
Ill
To the bangkero, who helped us get our water hyacimh in the Pasig river.
To Ms. Abigail P. Cid, our thesis adviser, for her unfailing help, support
and guidance throughout our thesis. And for her time and patience in correcting
our mistakes in our manuscript in order to finish and have a satisfying results.
To our panelist namely Engr. Liduvina Nery, Dr. Lonia Enerva, and
Dr. Joy Hofileiia, for their time even though they are very busy people amd for
iheir questions and advise which made us improve our thesis.
To our parents, family and friends for their love and support.
To my Mom for accompanying in Navotas port to get our seaweeds
sample and for willingly get the burden we have just to lighten ours (Cj).
To Mhark and Jet for being our inspiration. For their encouraging words
and their smiles that cheered up us to continue our thesis.
To all those people we have forgotten to mention, for their love, support
and prayers.
And most especially to our Almighty God for His blessings and guidance
vvhenever we cross the very dangerous Commonwealth Ave., just to able to reach
ihe PNRI and for giving us the people we have right now that helped and inspired
Table 4.2 shows the optimum pH of the 4 plant samples. Tamarind and water
hyacinth has an optimum pH of 7.5, spotted dracaena has 5.5 and grapes seaweed has 7.0.
23
Table 4.2 Optimum pH of the Different Plant SamplesSamples(n = 3)
TamarindWater Hyacinth
Spotted DracaenaGrapes Seaweeds
Optimum pH
7.57.55.57.0
The diluted standard HRP which ranged from 0.05 to 0.25 units ml"1 gave a
different shades of pink when it was added in the mixture of solution A and B. Graph 4.1
shows the HRP calibration curve which gave a regression equal to 0.9899, a slope (m) of
0.0644 and a y intercept (b) of 0.0418. This data was used to get the peroxidase activity
of the 4 different crude extract of the plants.
Graph J.1 Calibration Curve of Standard Horseradish Peroxidase
HRP Standard Calibration Curve y = o.O644x + O.O418
r2 = C.9801
10 12 14 16 18
ACTIVITY (U/ml)
20 22 24 26 23
Addition of the crude extract sample tissue from the mixture of solution A and B,
showed a color change. For the tamarind and water hyacinth it turned pink, spotted
24
Addition of the crude extract sample tissue from the mixture of solution A and B,
showed a color change. For the tamarind and water hyacinth it turned pink, spotted
dracaena turned red and grapes seaweed turned light pink. Spotted dracacna showed the
highest reroxidase activity due to its roots were younger compared to the others;
followed by the tamarind; then the water hyacinth samples gathered from Pasig River
was polluted which might have interfere during the analysis; and lastly the grapes
seaweed which is under algae family,. Three trials were made for every sample. Table
4.3 shows the following absorbance of four tissue samples at 510 nm and their respective
peroxiense activities.
Tablc 4.3 Peroxidase Activity and Absorbance of Different Varieties of PlantsSamples! n = 3)
TamarindWater Hyacinth
Spotted DracaenaGrapes Seaweeds
Absorbancc at 510 nm
0.2870.1681.5920.058
Peroxidasc Activity (li ml"1)
57.1129.39
361.053.77
According to Saunder [2], based on the color change of the sample mixtures; the
tamarind, water hyacinth and grapes seaweed can be classified under compound II while
spotted dracaena can be classified under compound III of peroxidasc-hydrogen peroxide.
For the protein determination, Bicinchohinic acid protein assay was adopted. The
standard working reagenis was green in color. Diluted standard BSA ranging from 5 to
250 (.ig m."1 gave different shades of purple after 30 minutes of incubation. Graph 4.2
shows the calibration curve of standard Bovine Serum Albumin.which gave a regression
equal to 0 9926, a slope (m) of 0.002184 and a y intercept (b) of 0.0418. This data was
used to get the total protein concentration and specific peroxidase activity of the 4
different crude extract of the plants.
25
Graph 4.2 Calibration Curve of Standard Bovine Serum Albumin
BSA Calibration Curve for Protein Determinationy=0.0022x+0.1715
r2 = 0.9853
£c
CO
oc
ow
100 200 300 400 500 600 700 800 900
Concentration (micro g/m!)
1000 1100 1200
Upon addition of the crude extract of the four samples in the standard working
reagent; lamarind and spotted dracaena immediately change their color to violet. The
sample mixtures were incubated at 60°C for 30 minutes. After the incubation, the mixture
gave different shades of violet. The sample mixtures were cooled at room temperature
and absorbance was read at 562nm. Spotted dracaena gave the highest absorbance,
followed by the water hyacinth, then the tamarind and lastly was the grapes seaweeds.
The lighter color was first read. Table 4.4 shows the absorbance at 562 nm, the total
protein concentration and the specific peroxidase activities of the samples. Low specific
peroxicla-e activity was obtained from the four crude extracts.
Table 4.4 Total Protein Concentration and Specific Activity of the Four Plant Samples
San pics(n = 3)
Tan.arindWater I lyacinth
Spotted DracaenaGrapes Seaweed
Absorbanccat 562 nm
1.0910.4852.6180.413
Total ProteinConcentration
(mg ml*1)421.02143.541119.96110.57
Specific PcroxidascActivity(U rag1)
• 0.13560.20480.32240.0341
P roxidase activities of the tissues that were kept at ultra low personal freezer for
four days were tested in the same manner, 'fable 4.5 shows the percent degradation of
zero old . JOI tissue to four day old root tissue samples. Analysis showed thai almost all
the pcrox dase in spoiled dracaena was degraded, while there was only slight degradation
on grape:- seaweeds.
Table 4.5 Percent Degradation of Pcroxidase Activity of the Four Plant Samples
Samples(n=3)
TamarindWale.- hvacinth
Spoiled dracaenaGrapes seaweed
Absorbance @ 510 nm0 days0.2870.1681.5920.058
4 days0.2340.1040.0790.055
% Degradation
38.H)%"95.04%5.17%
27
CHAPTER V
CONCLUSION AND RECOMMENDATION
From the analysis of the crude extracts of tamarind, spotted dracaena, grapes
sca'vecd and water hyacinth were stable at the optimum pH of 7.5, 5.5, 7.0, and 7.5
respectively
The peroxidase content of the plant varies with its location. Land plants such as
spoUed dracaena and tamarind gave high peroxidase activities than those of the water
plants. There were also variations in the extent of growth and high temperature of the
peroxidase in plants.
However, only four samples were analyzed. It is recommended that other plant
varieties should be studied to be able to determine which plant could be the best source of
peroxidape.
It is also recommended that the crude extract of the four samples that were
analyzed be subjected to isolation and purification to yield better results.
28
REFERENCES
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2. SUJNDERS, BC, HOLMES, AG AND STARK, BP. 1964. Peroxidase: TheProperties and Uses of Versatile Enzymes and of Some Related Catalyst, GreatBritain:Edlard & Son Limited, 240p.
3. EPONOMOU, A, SYROS, T, AND YUPSANIS, T. 2001. Factors Affecting theDetermination of Peroxidase Activity of Ebenus Cretica L. Cutting- APreliminary Survey. Propagation of Ornament Plants 1:50-53.
4. POKORA, A AND JOHNSON, M. 1993. US Patent #5, 178:762.
5. Revieved from the World Wide Web http://www.biotech.iastate.edu/publications/news_releases/march_9_00.html
6. YL. J AND TAYLOR, K. 1994. Phenol Conversion and Dimeric Intermediates inHorseradish Peroxidase- Catalyzed Phenol Removal from Water. EnvironSciTech 28: 2154.
7. Ret ieved from the World Wide Web http://www.purdue.edu/UNS/hcml/4ever/9605.Vierling.html
8. SOLOMON, S. 1987. Introduction to General, Organic and Biological Chemistry.New York: McGraw-Hill Co., Inc. p473,468.
9. SUP.ROTO. MA. 1994. Use of Plant Materials and Plant Organ Culture in WastcwaterTreatment. Annual Reports to ICBiotech 17: 335-336
10. WITITSUWANNAKUL, R. 1995. Pcroxidase and Lecithin from Rubber PlantationWaste. Annual Reports to ICBiotech 18: 289-290.
11. ROSSOMANDO, EP. 1990. Measurement of Enzyme Activity. In: Abclson, JN andSimon, MI editors. Methods in Enzymology: Guide to Protein Purification. SanDiego CA: Academic Press, Inc.,p38-67.
13. Grolicr: 1995. Encyclopedia of Knowledge 7: 76-79.
29
14. ATKINS, PW AND SHRIVER, DF. 1999. Inorganic Chemistry, 3r(1 ed. New York:Oxford University Press. p645-671.
15. MRTZ, CR. 1989. Schaum's Outline; Physical Chemistry, 2nd ed. New York:McGraw-Hill Co., Inc. pl47 - 148. '
\b. STOLL. VS and BLANCI1ARD, JS.1990. Buffer: Principles and Practice. In:\belson, JN and Simon, MI editors. Methods in Enzymology: Guide to ProteinPurification. San Diego CA: Academic Press, Inc., p24-31.
17. HOLLER, FJ, SKOOG, DA AND WEST, DM, 1996. Fundamentals of AnalyticalChemistry,7th ed. USA: Saunders College Publishing, pi42 - 1'44.
18 IIARGIS, L.I. 1988. Analytical Chemistry: Principles and Techniques. New Jersey:Pivnlice-Hall, pi3 17.
19. Retrieved from the World Wide Web htlp://www.oceanlink.island.net/oinfo''seaweeds/seaweeds.html
20. [RISR| Research Information Series on Ecosystem: 1992. Sampalok 2, 56p.
21. Retrieved from the World Wide Web http://www.hort.purd.ue.edu/newcrop/morton/tamarind.html#Description -description of tamarind
22. Retrieved from the World Wide Web http://www. Geocitics.com/CollegePark•'Lab/9619/wahyl. html
23. Retrieved from the World Wide Web http://www.ecy.wa.gov/programs/wq/plants'wceds/aquaO 10.html
24. Ret. ieved from the World Wide Web http://www.anamed.net/EngIish_Home_
Page/Water_Hyacintli/water_hyacinth.html
25. Ret. icved from the World Wide Web http://www.briansgarden.com/index.html
26. Retrieved from the World Wide Web http://www.freakinfucus.co.uk/primcrs/orim_basic.html
27. 1995 Grolier Encyclopedia of Knowledge'16:376-377.
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29. CAREY, FA. 1992. Organic Chemistry. New York: McGraw-Hill, Inc. p347.
30
30. J.^MERLARIN, E. 1995. Partial Purification of an Ionic Peroxidasc from PhilippineRadish (Raphanus sativus L.) [Undergraduate Thesis] Diliman, Quezon Cily,Philippines: UP Diliman. 49p. (Available at the UP Diliman-College of ScienceLibrary).
31. C'EGARRA, AC. 2001. Overproduction of Fungal Peroxidascs in FilamaentousFungi. Spanje: Geborente Cartagena, p. 12-13.
32. Retrieved from the World Wide Web http://www.unige.ch/LABPV/books/per-
appl/abstl .html.
33. Retrieved from the World Wide Web http://www.paracelsus-baclhall.at/arbcit.pdf.
34. UACI1, J1-' OAKL-NRJLL, D AND SMITH, MD. 1979. Increased Thermal Stabilityof Proteins'in the °rescnce of Sugar and Polyols. 29p.
35. I;SCANDF.I.OR. Cl'-. 1994. Isolation of Peroxidasc from Philippine Radish(Raphanus sativus L.) [Undergraduate Thesis] Dilim'an, Quezon City, Philippines:UP Diliman. 45p. (Available at the UP Diliman-College of Science Library).
36. f.OCOM, MY. 1995. Partial Characterization of Philippine Radish (Raphanus sativusL.) [Undergraduate Thesis] Diliman, Quezon City, Philippines: UP Diliman. 30p.(Available at ll'ic UP Diliman-College of Science Library).
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38. WORTHINGTON, M.1978. Enzyme and Related Biochemicals. Belford.MA:Milliporc Corporation. pl45-146.
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APPENDICES
APPENDIX A:
CALCULATIONS
1. pH Range Used in Detetmining the Optimum pH of the Different Varieties of Plant
Read at 510nm
1. Sample A (TAMARIND) .
pH
5.5A,
•J. Iz. /
6.0 | 0.1606.57.07.5
0.1140.2130.244
8.0 | 0.317
Trial 1Af
0.4060.3800.3500.5000.5310.587
AA0.2790.2200.2360.2820.2870.270
Trial 2A,
0.1310.1490.1150.1970.2620.310
Ar
0.4080.3590.3470.4540.5210.582
AA0.2770.2100.2320.2570.2590.272
TrialA,
0.1100.1270.1320.1850.2610.314
Ar
0.3970.3490.3600.4790.5760.554
AA0.2870.2200.2280.2940.3150.240
Ave.AA
0.286.02200.232
L0.2780.2870.271
2. Sample B (WATER HAYCINTH)
pH
5.56.06.57.07.58.0
A,0.0870.0970.0820.11 10.2190.284
Trial 1Af
0.2540.2320.2100.2330.3870.434
AA0.1670.1350.1280.122'0.1680.150
Trial 2A,
0.0900.0880.0890.1030.2470.238
Af
0.2510.2220.2240.2060.4220.384
AA0.1610.1340.1350.1030.1750.146
TrialA,
0.0870.092
. 0.0860.1550.2500.258
Af
0.2370.2270.220
.0.2880.4110.410
AA0.1500.1350.1340.1330.1630.152
Ave.AA
0.1590.1350.1320.1190.1680.149
3. Sample C (SPOTTED DRACAENA)
pH
5.56.06.57.07.58.0
Ai0.2450.3250.2180.3220.3200.369
Trial 1
121
Ar
015.880
1.525111
418.301.460
i
111101
4A770555307096981091
A,0.3410.2350.2310.2520.3990.355
Trial 2
1
ITlIil
Ar
801745.488506.394609
i
I11101
\A460510257254995254
A,0.2430,2490.2480.3100.3490.382
Trial
111111
Af
789659.617.488579594
111111
AA.546.410.369.178.230.212
Ave.AA1.5921.4921.3111.1761.0691.187
4. Sample D (GRAPES SEAWEEDS)
p j j
5.56.06.57.07.58.0
Trial 1Ai
0.001NANA
0.0600.1830.237
Af
0.0220.001NA
0.1090.2240.283
AA0.0210.003ND
0.0490.0410.046
Trial 2Ai
0.015NANA
0.1000.1500.249
Af
0.045NANA
0.1650.2130.302
AA0.031NDND
0.0650.0630.053
A;„ NA" NA
NA0.0750.1890.221
TrialAf
NANANA
0.1250.2350.287
AANDNDND
0.0500.0460.066
Ave.AANDNDND
0.0580.0500.055
NA- no activityND- not determined
II. HRP STANDARD CURVE
Activity(U.rar1)
0.05
0.10
0.15
0.20
0.25
Absorbance at 510 nm
Trial 1A,
0.142
0.135
0.129
0.146
0.150
Af
0.193
0.193
0.175
0.207
•0.212
AA0.051
0.058
0.046
0.061
0.062
Trial 2A,
0.111
0.122
0.131
0.133
0.099
Af
0.152
0.166
0.181
0.188
0.143
AA0.041
0.044
0.050
0.055
0.044
Trial 3Ai
0.100
0.133
0.166
0.144
0.141
Af
0.142
0.179
0.222
0.191
0.210
AA0.042
0:046
0.056
0.047
0.069
Ave.AA
.0.0447
0.0493
0.0507
0.0543
0.0583
Aj= initial absorbance Af= final absorbance AA= change in absorbance
III. BSA STANDARD CURVE FOR PROTEIN DETRMINATIONConcentration (iig.ml"1)
5102550100250
Absorbance at 562 nm0.1490.1820.2320.3060.4200.701
IV. PROTEIN DETERMINATION OF THE SAMPLESSample
Tamarind
Water HyacinthSpotted Dracaena
Seaweeds
Absorbance at 562 nmTrial 11.068
0.4882.2670.425 i
Trial 21.1140.4832.9680.401
Average Abs.1.0910
- 0.48552.61750.413
APPENDIX B:
PICTURES
I. SAMPLING SITES
TAMARIND
Vicinity of Philippine Nuclear Research Institute
WATER HYACINTH
Along the shore of Pasig River in Mandaluyong City
y *^^*.^r.^SajHippffiP'fffJArfflL''I-L"•^•f«'i"j^^BSKWsfWnS^^fiS^^^^^^^^^^^^v^^w^^^'^^^?^^
SPOTTED DRACAENA
At a residence backyard
H
SEAWEED
At Navotas Port
II. APPARATUS USED
A;™
UV-Vis Spectrophotometer
pH meterwith magnetic stirrer
Optimum pH Set - up
MicrocentrifugeMachine
Ultra LowPersonal Freezer
Plant Root Tissues Crude Enzyme Extract
III. PEROXIDASE ACTIVITY
Differerit Concentrations of Standard HRP Color Reaction of the MixtureUpon Addition of Crude
Extracts
IV. BICINCHONINIC ACID PROTEIN ASSAY
BEFORE(0 hours)
AFTER(2 hours)
if
Different Concentration of Standard BSA
Color Reaction of the SWRUpon Addition of Crude Extract
APPENDIX C:
CERTIFICATIONS
CERTIFICATION
Republic of the PhilippinesOffice of the President
NATIONAL MUSEUM'P. Burgos Street, Manila 1000
To whom i I may concern:
This is to certify that the specimens from'-KftaK \)vicx.w\ jfrJ._- A o ^ C - VAQA\v\QtUA Of Pclyl^hnio l/ni wrtf/h) •'Of <lw Phi'lippW Wereidentified :is Allows:
Scientific Name Local Name, Family Name
l ; w . ^yc\oV-t\<w\ iJcGUHWio^A^/t^ilOylAhlK I
This certification is issued for any scientific purpose it may serve.it
Determined by:
DR. ^ILEREDO^1. VENDIVILSenior Researcher..F.S.: Medicinal PlantsTaxonomy of Flowering PfatitsTerrestial and Mangrove Ecosystem
The lenn AUTHENTICATION is only applicable for antique items -As pwl ot'the National Musemn and Dr. W.F. Vaidivil public services for the promotion
HEAJD OFFICE of science, this certification is FREE OF CHANGE -Tel. Noj.:
Republic of the PhilippinesOffice of the President
NATIONAL MUSEUM
P. Burgos Street, Manila 1000 ,
To whom it may concern:
This is to certify that the specimens from Mqoo Pyp.on ^- IVC'-ef.- - T- Joy c lyiqnhboy of polytechnic Unnw-^y "cf H"ve Ptv^
identified as f611ows: / 'were
Scientific Name Local Name Family Name
^y
This certification is issued for any scientific purpose it may serve.
Determined, by:
^ 7 0 7 / /DR.VWILFREDO*F. VENDIV1LSenior ResearcherF.S.: Medicinal PlantsTaxonomy of Flowering PlantsTerrestial and Mangrove Ecosystem
HEAD OFFICETel. Nos.:
527-1215527-1242527-1209
Fax No. :527-0306
e-mail : nmuMMm>(i<)i-nexl.tiet
The lerm AIJTIIKNT1CATION is only applicable for antique items -As part of the national Museum mid Dr. W.R Vendivil public services for the promotiono l scie,,ce, this certification is FREE OF CHARGE -
624 BARRIO SAN JOSE,MANDALUYONG CITYTELEPHONE #578-8341MOBILE #09216035502
MAAN DYANN NUGUID BEROSIL
EDUCATIONAL BACKGROUND
2001 - Present
1997-2001
1991 -1997
Bachelor of Science in ChemistryPciytechnic University of the PhilippinesMabini Campus, Sta Mesa, Manila
CITY OF MANDALUYONG SCIENCE HIGH SCHOOLE. Pantaleon Street, Hulo, Mandaluyong City
FILEMON P. JAVIER ELEMENTARY SCHOOLBarrio San Jose, Mandaluyong City
PERSONAL BACKGROUND
AGEDATE OF BIRTHPLACE OF BiRTHCIVIL STATUSHEIGHTWEIGHTCITIZENSHIPFATHER'S NAMEOCCUPATIONMOTHER'S NAMEOCCUPATIONRELIGIONLANGUAGE SPOKENSKILL
FilipinoGerardo L. BerosilTaxi DriverShirley N. BerosilHousewifeRoman CatholicFilipino and EnglishComputer Literate
879 E. PANTALEON STREET,MANDALUYONG CITYTELEPHONE # 579-8729
CHERRIE JOY CALALAY IWAGTIBAY
EDUCATIONAL BACKGROUND
2001 - Present
1997-2001
1991 -1997
Bachelor of Science in ChemistryPolytechnic University of the PhilippinesMabini Campus, Sta Mesa, Manila
CITY OF MANDALUYONG SCIENCE HIGH SCHOOLE. Pantaleon Street, Hulo, Mandaluyong Ciiy
St. John's AcademySan Juan, Metro Manila
PERSONAL BACKGROUND
AGEDATE OF BIRTHPLACE OF BIRTHCIVIL STATUSHEIGHTWEIGHTCITIZENSHIPFATHER'S NAMEOCCUPATIONMOTHER'S NAMEOCCUPATIONRELIGIONLANGUAGE SPOKENSKILL
19 yearsOctober 27, 1984MandaluyongSingle5'46.82 kgs.FilipinoLeovino S. MagtibayDentistLucila C. MagtibayNurseRoman CathodeFilipino and EnglishComputer Literate