September 25, 2009 Organotypic Tissue Culture Organotypic Tissue Culture for Substance Evaluation for Substance Evaluation Potsdam, September 2009
September 25, 2009
Organotypic Tissue Culture for Organotypic Tissue Culture for Substance EvaluationSubstance Evaluation
Potsdam, September 2009
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WHY WE ARE HERE (PART I)
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WHY WE ARE HERE (PART II)
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Single Organs Multiple Organs• Skin
• Liver
• Lymph Node
• Pancreas
• Heart/Muscle
• Trachea/Lung
• Gut/Intestine
• Tumor
• Single neurons
• Liver/GI
• Kidney/GI
• Neural cortex/bone marrow/stem cells/circulation
BEYOND CELLS TISSUES & ORGANS
DISCOVERIES
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• The meaning of life? Reaction and communication
• “Single cells are destined to die”
• Life is different at the micro- and nahnoscale
• Dynamics create challenges as well as opportunitieso Flow creates evaporation and removal as well as circulation and nutrition
• Skin is a pioneering exampleo Monolayer Full Thickness (FT)
o Short-term culture One year in culture!
o R&D Commercial with multiple vendors
FRONTIERS (SCIENTIFIC)
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• Use the environment more• Cross the species barrier • Go nano
o Use nanomaterials to deliver co-factors like Ca+2 o Use nanoparticles to enhance transport
• Seek to scaleo Scale down: micro- and nanogeometries may be differento Scale up: 1+1 = ½?
• Co-culture is crucial even in single-organ systems (pancreas – 4% of cells may be the key)• Adding cell types, circulation, neural connections, adipocytes – not easy but necessary
o Adipocytes to skino Stroma to pancreas; stroma to lymph nodeo Endothelial cells to lymph node
FRONTIERS (COMMERCIAL)
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• Not just normal but also disease models• Think not only “in vitro” but “in silico”• Think “manufacturability”• Think “modular”• Think “multiple price points”• Find immediate needs to satisfy • Seed it with stem cells & iPS cells
A PATH FORWARD
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Intel chip ca. 1971 Intel chip 2010
ChemistryBiologyTechnology
September, 2009
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