cbit.ac.in - . | CHAITANYA BHARATHI INSTITUTE OF ...cbit.ac.in/files/B.TECH BIOTECH II YR II SEM.doc · Web viewAdvanced engineering Mathematics (Fifth edition) Wiley Eastern, Erwin

BIOTECHNOLOGY SYLLABUS

SCHEME OF INSTRUCTION AND EXAMINATION

B.Tech. (Biotech) - II YEAR -II Semester

SUBJ.CODESUBJECTS PERIODS /

WEEK

The /Lab

MARKSDURATION OF EXAM.

Hrs

SessionalExams Hours

BTT.2.201 Mathematics – 114 30 70 3

BTT.2.202 Microbiology4 30 70 3

BTT.2.203 Genetics and Molecular Biology

4 30 70 3

BTT.2.204 Electrical and Electronics Engineering

4 30 70 3

BTT.2.205 Instrumental Methods in Biotechnology

4 30 70 3

BTT.2.206Industrial

Biotechnology 4 30 70 3

BTP.2.207 Microbiology Lab4 25 50 4

BTP.2.208 Electrical & Electronics Engineering Lab.

4 25 50 4

BTP.2.209 Instrumentation Lab.4 25 50 4

355 570

MATHEMATICS - II

Subject code : BTT.2.201 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT - I.

Complex numbers, curves and regions in complex plane. Function of a complex variable. Limit, continuity, Derivative of function of complex variable. Analytic functions. Harmonic Function, Cauchy - Reimann equations.

UNIT - II.

Mapping by elementary functions, conformal mapping, Mapping of the functions

W = Zz, W = ez, W = 1/z, W = Z +l/z, W = Sin Z; and W = Cos z. Bilinear transformation.

UNIT - III.

Line integral in the complex plane. Basic properties of the complex line integral. Cauchy's theorem, Cauchy's integral formulae. Evaluation of line integral.

UNIT - IV.

Taylor's theorem. Taylor's series of elementary functions. Laurent's theorem, Laurent's expansion of functions. Analyticity at infinity, zeroes and singularities.

UNIT - V.

Revidues - calculation of residues. Integration by the method of residues.

Contou integration (J2^ / ( Cos 0, Sin 6 ) d 0 ; J / (x)dx) O -00

EXAMINATION : One question from each unit with internal choice.

TEXT BOOKS:

1. Higher Engineering Mathematics, by B.S. GAREWAL, Khanna Publishers ( (1998)

2. Advanced engineering Mathematics (Fifth edition) Wiley Eastern, Erwin Kreysizig,

MICROBIOLOGY




UNIT- I: HISTORY AND INTRODUCTION TO MICROBIOLOGY

Historical Developments in Microbial; Biochemical tests-Sugar fermentations, IMVIC tests, Catalase production etc.Contributions of Antony Van Leiwenhoek, Louis Pasteur, Robert Koch, Iwanowskii, Edward Jenner; Outline of historical development in microbial biotechnology applications (Food, Pharma, Environmental and industrial applications) Identification of Microorganisms: Staining techniques; Principle dyes, Fixation, simple staining, differential staining, spore staining and flagella staining.

UNIT- II MAJOR GROUPS OF MICROORGANISMS

General and Colony characters of major groups of Microorganisms - Algae, Fungi, Protozoa, Bacteria; Identification of Microorganisms by Major Taxonomical Characteristics (Morphological, Physiological, Ecological, Cultural, Metabolic/Biochemical, Immunological and Genetic). Classification of Microorganisms - Concept of Classification; Taxonomic Groups; Haeckel's three kingdom concept, Whittaker's five kingdom concept, Three domain concept of Carl Woose.

UNIT- III: VIRUSES AND THEIR CHARACTERS

Genera! Characters of Viruses and Life cycles (Lytic and Lysogenic); Structure and Replication of Bacteriophage (T2) and Lamda; Tobacco Mosaic Virus (TMV), Retrovirus, HIV and Prions; Classification of Viruses - Major Characteristics for Classification, Characteristics of each group; Methods of Cultivation of Viruses; importance of Viruses in Biotechnology.

UNIT- IV: MICROBIOLOGICAL TECHNIQUES

Methods of Culturing of Microorganisms in Lab and Industry - Culture media, (Liquid, Semi-solid and Solid media, Synthetic media and Complex media), Types of media (simple, routine lab, selective, differential, enrichment and enriched), Isolation of pure cultures (Streak, Spread and pour plate methods). Concept of Sterilization - Methods and Their Application- Physical Methods (Heat, Filtration and Radiation), Chemical methods (Phenolics, Alcohols, Halogens, Heavy metals, Dyes, Quaternary Ammonium compounds,, Aldehydes, Gaseous agents); Methods of Preservation of Microorganisms and their importance; Maintenance and preservation of bacterial cultures.

UNIT-V: MICROBIAL PHYSIOLOGY AND GROWTH

Nutrition in Microorganisms and Assimilation of Major Nutrients; Nutritional groups of microbes and their importance in fermentation Industry; Microbiological Media and Their Application Growth of Microorganisms - Growth factors, Growth curve, Mathematical expression of growth, Measurement of microbial growth (Cell numbers and Cell mass), Importance of Growth Phases of Microorganisms; Balanced and unbalanced growth, factors affecting bacterial growth (Solutes, Water activity, pH, temperature, oxygen concentration, osmotic pressure, radiation).

EXAMINATION : One question from each unit with internal choice

TEXT BOOKS:

1. Microbiology, 5th edition, Pelczar, Michael J, Krieg, Noel R.; Jr.; Chan, E. C, McGrawHill. (1993).

2. Textbook Of Microbiology by Dubey Rc, Maheshwari Dk S Chand & Company Ltd. (2006).

3. Industrial Microbiology- Prescott and Dunn.4th edition, CBS Publ.& Distributors.

REFERENCE BOOKS:

1. Prescott L.M. Harley J.P Klein D.A Microbiology,6 th ed, (2004), McGraHillBrooks G.F, Butel J.S., Morse S.A., Medical microbiology, 23rd ed McGraHill (2004).

2. Johnson A.G., Ziegler. R.J Microbiology and Immunology,4 th ed , ((2002)), Lippincott Williams & Wilkins publishers, Baltimore.

3. Microbiology, 5th edition, Pelczar, Michael J, Krieg, Noel R.; Jr.; Chan, E. C, McGraw-Hill.

4. Principles of Microbiology, Atlas, RM, 2nd edition, W.M.C. Brown Publishers, Dubuque,(1996)

5. Microbiology: An Introduction by Gerard J. Tortora, Berdell R. Funke, Christine L. Case. Pub: Benjamin Cummings; 10th edition (2009).

6. Alcamo's Fundamentals of Microbiology, Effrey C. Pommerville 8th Edition Jones & Bartlett Publishers; 8th edition (2006).

7. Benson's Microbiological Applications: Laboratory Manual in General Microbiology, Short Version by Alfred Brown McGraw-Hill Science/Engineering/Math; 11th edition (2008)

8. Microbiology by Prescott/Harley/Klein's Joanne Willey, Linda Sherwood , Chris Woolverton, McGraw-Hill Science/Engineering/Math; 7th edition, (2007).

GENETICS AND MOLECULAR BIOLOGY




UNIT-I: PHYSICAL BASIS OF HEREDITY

Baisc laws of inheritance, mono, di, tri, hybrid ratios, Mendel’s laws, Linkage and crossing over: cytological basis, Identification of genetic materials: classical experiments by Griffith, Avery, Mc Cleod, Hershy Chase etc. RNA as genetic material, pseudo genes, overlapping and split genes.

UNIT-II: GENETIC RECOMBINATION, MAPPING & MUTATIONS

Transformation, conjugation, F-mediated sexduction, generalized, specialized transduction, mechanism of recombination & recombination frequencies; Mapping of bacterial chromosomes and construction of genetic map of E.coli Mutations: Spontaneous, induced, lethal mutagens, their types & actions, classification of mutations, characters of mutations and applications. Maryleon's hypothesis, sex linked disorders in human beings.

UNIT-III: DNA STRUCTURE, REPLICATION & REPAIR

Structure of DNA-Watson & Crick's model; Types of DNA- A-DNA, B-DNA, Z-DNA; Denaturation and renaturation of DNA. Replication of DNA- Semi conservative replication, enzymology of replication, continuous and discontinuous DNA synthesis, complex replication apparatus, unidirectional replication, bi-directional replication, rolling circle replication; DNA damage and repair: Types of DNA damages- deamination, alkylation, pyrimidine dimmers; Repair mechanisms-Excision repair and mismatch repair, SOS repair, rec gene and its role in DNA repair.

UNIT-IV: MECHANISM OF TRANSCRIPTION

Structure of Promoters- RNA Polymerases of Prokaryotic and Eukaryotic Organism; Transcription- Initiation, Elongation and Termination; Post Transcriptional Processes of Eukaryotic RNA; Structural features of RNA- rRNA, mRNA, tRNA and functions of RNA. Transcription apparatus and proteins involved in transcription. Prokaryotic & Eukaryotic

transcription. Processing of t-RNA, r-RNA, m-RNA splicing; Concept of Ribozyme, Inhibitors of Transcription.

UNIT-V: MECHANISM OF TRANSLATION

Ribosome- Structural features of prokaryotic and eukaryotic ribosome; Genetic code-Triplet code, cracking of genetic code, features of genetic code, wobble hypothesis; Protein synthesis: Translation in prokaryotes and eukaryotes- initiation of translation, elongation of polypeptide chain, termination of translation. Post translation modification, protein transport, Inhibitors of Protein Synthesis.

EXAMINATION : One question from each unit with internal choice

TEXT BOOKS:

1. Principles of Genetics. Gardner, E. J., Simmons, M. J., Snustad, D. P. and Snustad, (1985). John Wiley and Sons, Inc.

2. Genetics -BD Singh 3rd edition. Kalyani Publications 3. Cell and Molecular Biology: Concepts and Experiments by Gerald Karp. Wiley; 4th

Edition (2004).4. Molecular Biology -Friefelder, 2nd edition Narasa Publ, (2007).

REFERENCE BOOKS:

1. Principles of Genetics-Robert H.Tamarin, Tata McGraw Hill 6th edition (1998).2. Molecular Biology of the Gene, Fifth Edition by James D. Watson , Tania A. Baker,

Stephen P. Bell, Alexander Gann , Michael Levine , Richard Losick ,. Benjamin Cummings; 5th edition (2003).

3. Molecular cell biology – Lodish H., Berk A, Matsudaira P., Kaiser CA., Krieger M., Scott MP., Zipurski SL., and Darnell J5th ed. WH Freeman & Co. (2003).

4. Genetics and Molecular Biology by Dr. Robert F. Schleif. The Johns Hopkins University Press; 2nd edition, (1993).

5. Molecular Biology of the Cell by Bruce Alberts , Alexander Johnson , Julian Lewis , Martin Raff , Keith Roberts, Peter Walter. Garland Science; 5th edition

6. Genetics and Molecular Biology by Dr. Robert F. Schley, The Johns Hopkins University Press; 2nd edition, (1993).

7. Schaum's Outline of Genetics by Susan Elrod, McGraw-Hill; 4 Editions, (2001).8. Genetics: Analysis of Genes and Genomes by Daniel L. Hartl, Elizabeth W. Jones. Jones

& Bartlett Publishers; 6th Edition, (2004).9. Real-Time PCR: Current Technology and Applications. Julie Logan, Kirstin Edwards and

Nick Saunders Academic Press. (2009).10. Molecular Biology of the Cell By Maurice Wilkins Publisher: Garland Science (2002).

ELECTRICAL AND ELECTRONICS ENGINEERING




UNIT - I.

D.C. Circuits and network theorems: Kirchoff s laws - mesh current and node voltage analysis - superposition theorem - thervenin's theorem - Norton's theorem - maximum power transfer theorem.

UNIT - II.

ElectromagneticInduction: Electromagnetic Induction - faraday's laws of electromagnetic induction -Direction of Induced e m f and current - Induced e m f self inductance - Mathal inductance - coupling co-efficient Rise of current in an Inductive circuit - decay of current in an inductive circuit - energy stored in a magnetic field - Hysteresis and eddy current losses. D.C Generators generator principle action of commentator construction of D.C. Generator - types of armature winding E.M.F. equation - types of generators - losses and efficiency - armature reaction -characteristics of different types of generators

UNIT- III.

D.C Motors: working principle - Back E M F voltage and power equation - Types of D.C. Motors torque and speed of a D.C motor - losses and efficiency characteristics of different types of D.C. Motors - speed control of D.C. shunt and series motors shunt motor starter.

A.C. Circuits: Generation of alternating voltages and currents - equation of alternating voltage and current average and r m s value of sinusoidal quantities - form and peak factor - pharos representation of sinusoidal quantities - addition and subtraction of alternating quantities - A.C. circuit containing resistance only Inductance only - capacitance only -R-LR-C,R-L~C, series circuits - parallel A.C circuits.

UNIT -IV.

Single phase transformer: Constructional details - working principle of an Ideal transformer - E M F equation - practical transformer on no load and on load - equivalent circuit - voltage regulation - losses and efficiency - condition for minimum efficiency - open circuit and short

circuit test. Three phase Induction Motors: advantages and Disadvantages - Construction - Principle of production of rotating magnetic field - working principle of Induction motor - slip rotor current frequency effects of slip on the rotor circuit - rotor current starting torque condition for minimum starting torque - induction motor under load - torque under running conditions - Full load Torque - Torque - slip characteristics -Losses and Efficiency.

UNIT -V.

Electronic Devices & Circuits:- P- n Junction - Semiconductor diode - characteristics of diode – Diode as rectifier - Half wave - Full wave rectifier - Bridge rectifier - Filter circuits. Transistor - Construction - Transistor action Transistor symbols - Transistor as an amplifier – common base connection of a transistor - characteristics of common base connection - common emitter connection of a transistor - characteristics of common emitter connection transistor as an amplifier in C E arrangement.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Principles of Electrical engineering and Electronics by V.K. Mehta. S.Chand & Co. (1996) Edn.

2. Basic Electrical And Electronics Engineering (2007) by R.k. Rajput Publisher: Laxmi Publications

REFERENCE BOOKS:

1. Electrical Technology, B.L. Theraja & A.K. Theraja, S Chand & Co.2. Teach Yourself Electricity and Electronics, Fourth Edition by Stan Gibilisco McGraw-

Hill/TAB Electronics; 4 edition, (2006).3. Electronic and Electrical Engineering: Principles and Practice by L.A.A. Warnes.

Palgrave Macmillan; 3Rev Ed edition, (2002).4. Bird, J.O. Electrical and Electronic Engineering Principles. Longman 19875. Boylestad, Robert L. Introductory Circuit Analysis. Prentice-Hall (2003) 10th Edition.6. Digital Electronics: Principles, Devices and Applications.Anil Kumar Maini, Wily

Eastern. (2007).7. Industrial Electricity and Motor Controls by Rex Miller, Mark Miller, McGraw-Hill

Professional; 1st Edition (2007).

INSTRUMENTAL METHODS IN BIOTECHNOLOGY




UNIT-I: ANALYTICAL METHODS

Types of Analytical Methods - Instruments for Analysis - Uncertainties in Instrumental measurements - Sensitivity and detection limit for instruments.

UNIT-II: INSTRUMENTS FOR ISOLATION TECHNIQUES

Cell disruption by French press, Sonification, freeze thaw technique; use of liquid N2 and chemical approaches involved in cell disruption; Isolation of Biomolecules and cell organelles: centrifugation; basic principles of sedimentation, sedimentation coefficient, Svedberg Unit; various types of centrifuges, their uses, rotors, fixed angle, vertical, swing out, zonal rotors; preparative centrifugation, differential density gradient centrifugation, analytical ultra centrifugation; Materials used in preparation of density gradient- sucrose & cesium chloride; Isolation of sub cellular organelles and Biomolecules. Determination of molecular weight and purity of Biomolecules by analytical ultra centrifugation.

UNIT-III: SEPARATION TECHNIQUES

Partition coefficient, partition chromatography, counter current distribution, adsorption chromatography, Paper, TLC& GLC, adsorption media, solvent, continuous and gradient elution, fraction collection and detection of pure molecules. Methods based on size: Gel permeation chromatography, principle application- Molecular weight determination. Dialysis and its significance. Affinity chromatography, application & technique for purification of proteins and nucleic acids.

UNIT-IV: CHARGE BASED SEPARATION TECHNIQUES

Principle and application of Ion exchange chromatography, use of ion exchange- cation & anion exchangers, pH and salt gradients for elution of proteins, amino acids and nucleotides. Electrophoresis: Migration of charged molecules in electric field-moving boundary, paper, cellulose acetate, starch gel electrophoresis, SDS PAGE, Determination molecular weight, iso-electric focusing and its significance. Identification of specific proteins by western blotting. Agarose gel electrophoresis- separation of DNA & RNA, by agarose gel electrophoresis,

recovery of DNA fragments from agarose gels, southern & northern blot techniques and their significance, pulse field gel electrophoresis.

UNIT- V: SPECTROMETRIC IDENTIFICATION TECHNIQUES

Basic concepts of spectroscopy, Visible & UV spectroscopy & Explain Beer lamberts law; Principles and application of Colorimetry & Flame photometry, Nephlometry; Principles and applications of Atomic absorption Spectrophotometry; Principles & applications of IR, ESR NMR & Mass spectroscopy; Explains the laws of photometry.

Examination: One question from each unit with internal choice.


TEXT BOOKS:

1. Fundamentals of Analytical Chemistry- 8th edi. Skoog DA, West DM and others Thomson Brooks/Cole Eastern Press Bangalore, (2005).

2. Instrumental Methods of Chemical Analysis- GW Ewing 4th edition Mc Graw Hill. (1985).

3. Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson , John Walker. 6th edition Cambridge University Press. (2005).

REFERENCE BOOKS:

1. Readings in Scientific American, W.H.Freeman, (1985)-(1993).2. Instrumental Methods for Quality Assurance in Foods (Food science & technology) by

Daniel Y.C. Fung (Editor), Richard F. Matthews CRC Press; 1st

Edition 1991.

3. Basic Laboratory Methods for Biotechnology, 2/E Lisa A. Seidman, Madison Cynthia J. Moore, Publisher: Benjamin Cummings, (2009).

4. Separation processes in the food and biotechnology industries By A. S. Grandison, Michael John Published by Woodhead Publishing, (1996)

5. Near-infrared applications in biotechnology By Ramesh Raghavachari , CRC Press, (2001).

6. Natural products isolation By Richard J. P. Cannell , Published by Humana Press, (1998).7. Biotechnology and Fermentation Process. By Mr. John I. D'souza, Mr. S. G. Killedar

Published by Pragati Books Pvt. Ltd., (2008)8. Analytical biotechnology By Thomas G. M. Schalkhammer, Published by Birkhäuser,

(2002).

9. Chromatography: concepts and contrasts By James M. Miller Edition: 2, Published by John Wiley and Sons, (2005).

10. Thin layer chromatography in chiral separations and analysis By Teresa Kowalska, Joseph Sherma, CRC Press, (2007)

11. Microscopic techniques in biotechnology By Frank Mayer, Michael Hoppert Published by Wiley-VCH, (2003).

12. Agricultural biotechnology By A. Altman, Rita R. Colwell Published by CRC Press, 1997

13. Animal cell biotechnology: methods and protocols. By Ralf Pörtner Edition: 2, illustrated, Published by Humana Press, (2007).

14. Food analysis by HPLC By Leo M. L. Nollet Edition: 2, Published by CRC Press, (2000).15. Modern instrumental analysis By Satinder Ahuja, Neil D. Jespersen Published by

Elsevier, (2006).16. Fundamentals of contemporary mass spectrometry, By Chhabil Dass Published by Wiley-

Inter Science, (2007).

INDUSTRIAL BIOTECHNOLOGY




UNIT-I: INTRODUCTION TO BASICS OF BIOTECHNOLOGY

A historical overview on scope and development of biotechnology and products; Biotechnology as an interdisciplinary enterprise; A brief survey of organisms, processes, products and market economics relating to modern industrial biotechnology; Concepts of tools and techniques used in biotechnology; Areas of application of biotechnology.

UNIT-II: INTRODUCTION TO INDUSTRIAL BIOPROCESSES

Role of a bioprocess engineer in the Biotechnology Industry; Introduction, Development and Maintenance and characterization of Industrial Microorganisms; Primary and Secondary Screening of Innoculum, starter and industrial cultures, Analysis of microbial fermentation processes; Batch and Continuous fermentations, process controls, oxygen supply and demand, single and multiple bubble aeration, sparger aeration, foam control equipment, scale-up of Fermentors; An overview of aerobic and anaerobic fermentation processes.

UNIT III: PRODUCTION OF PRIMARY AND SECONDARY METABOLITES

A brief outline of processes for the production of some commercially important Organic acids (e.g. Citric acid and Lactic acid); Amino acids (Glutamic acid and Lysine); Alcohols (Ethanol, and n-Butanol). Study of Production processes for various classes of low molecular weight secondary metabolites-" Antibiotics, Beta-lactams (Penicillins), Amino glycosides (Streptomycin), Macrolides (Erythromycin), Quinones, Aromatics and Vitamins (B12).

UNIT-IV: PRODUCT DEVELOPMENT

Production of beverages (Beer,wine), Production of commercially important industrial enzymes, Production of recombinant proteins : insulin interleukins, Tumor necrosis factor.

UNIT- V: SPECIALITY BIOPRODUCTS FOR AGRICULTURAL, FOOD AND PHARMACEUTICAL INDUSTRIES

Bio-pesticides; Bio-fertilizers and Plant growth factors; Natural Biopreservatives (Nisin); Biopolymers (Xanthan gum and PHB); Single cell protein; High fructose corn syrup; Bioconversion of vegetable oils; Production of semi-synthetic penicillins and cephalosporins; Racemically pure drug intermediates; Steroid bioconversions.


TEXT BOOKS:

1. Biotechnology: Text Book of Industrial microbiology - CruegerW & CruegerA. 2nd edition, Panima Pub, (2005).

2. Industrial Microbiology, LE Casida. 1st edition, New Age Int, (2006).3. Industrial Microbiology, AH Patel. Mc Millan India ltd., (2007).4. Industrial Biotechnology Author: R. Singh, S.k. Ghosh Publishing: (2004).

Pub: Global Vision.

REFERENCE BOOKS:

1. Industrial Microbiology- Prescott and Dunn.4th edition, CBS Publ.& Distributors.2. Microbial Biotechnology: Fundamentals of Applied Microbiology -Glazer AN and Nikaido. 2nd edition, Cambridge University Press.3. Principles of Fermentation Technology- Stanbury PF, A Whitaker &. SJ Hall 2nd edition Elesvier Science Ltd.4. Organic Chemistry of Natural Products-OP Agarwal Vol I & II Goel Publishing House.5. Industrial Biotechnology P.R. Yadav, (2005) Pub. Discovery Publishing House6. Industrial Biotechnology Varun Shastri : (2006) Publisher: Isha Books7. Industrial Enzymes and Their Applications by Helmut Uhlig, Wiley-Interscience (1998).8. Industrial Biotechnology by Shara L. Aranoff, Robert A. Rogowsky, Karen Laney-Cummings, Deanna Tanner Okun, Daniel R. Pearson, Nova Science Publishers, Incorporated, Pub. (2009).9. Industrial Biotechnology Shara L Aranoff; Daniel R Pearson; Deanna Tanner Okun; Irving A Williamson; Dean A Pinkert; Robert A Rogowsky; Karen Laney-Cummings,Pub: Nova Science Publishers, Inc. (2009).10. Industrial Pharmaceutical Biotechnology Klefenz, Heinrich, Wiley-VCH ((2002).

MICROBIOLOGY LABORATORY

Subject code : BTP.2.207 Sessional : 25


Nature of Examination : Practicals Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

(Minimum of 8 Experiments in the list are to be performed)

1. Microscopic Observation, Staining and Identification of Bateria, Fungi, Algae' and Protozoa -

Identification of microorganism:

(a) Simple & Differential staining techniques (b) Biochemical testing

2. Calibration of Microscope & Measurement of Microorganisms- Microtomey.

3. Sterilization techniques (Autoclaving, Hot Air Oven, Radiation and Filtration)

4. Preparation of culture media (a) broth type of media (b) Agar

5. Culturing of microorganism (a) broth (b) pure culture techniques- Streak, Pour plate, flask,

Isolation and preservation of bacterial culture.

6. Antibiotic tests- Disc diffusion method, minimum inhibitory concentration.

7. Biochemical tests- IMIVC test, Catalase, Coagulase test, Gelatinase test, Oxidase.

8. Factors affecting the bacterial growth and study of growth curve

9. Measurement of Microbial Growth (Viable Count and Turbidometry)

10. Hanging drop technique

11. Colony counters- Enumeration of Bacterial numbers by serial dilution

12. Identification of animal and plant bacterial cells

13. Bacteriological Analysis of Foods

14. Yeast and Mould count in foods

BOOKS:

1. 1. Laboratory Experiments in Microbiology by M Gopal Reddy et al Himalaya Publishing House PVT Ltd.

2. Laboratory manual in Microbiology-P Gunasekaran, New Age International Publ., New Delhi.

3. Laboratory manual in General Microbiology NKannan ., Panima Publ. Corp., New Delhi.

ELECTRICAL & ELECTRONICS ENGINEERING LAB.




LIST OF EXPERIMENTS

(Minimum of 8 Experiments in the list is to be performed)

1. Verification of Theremins & Norton’s theorems

2. Verification of Maximum Power Transfer theorem & superposition theorem

3. Power factor measurement of and R - L series circuit

4. Calibration of single phase Energy meter

5. Magnetization Characteristics of separately excited generator

6. Load characteristics of shunt generator

7. Swin burnes Test on D.C shunt Motor

8. Seed Control of D.C Shunt Motor

9. Brake Test on D.C Shunt Motor

10. Brake Test on Induction motor

11. Open-circuit & short circuit Tests on single phase transformer

12. Static Characteristics of junction diode

13. Static characteristics of a common base & common emitter transistor circuit.

14. Brake test on D.C Series motor

15. Three - phase power measurement by two watt meter method

16. Regulation of single phase alternator

INSTRUMENTAL METHODS OF ANALYSIS LABORATORY




LIST OF EXPERIMENTS

(Minimum of 8 Experiments in the list are to be performed)

1. The calibration of pH meter and measurement of pH for different solutions.

2. Estimation of Ascorbic acid by colorimetric assay.

3. Estimation of unknown samples by using conductivity meter.

4. Estimation of different macromolecules by visible spectrophotometer.

5. Verification of Lambert - Beers law by UV -VIS spectrophotometer.

6. Estimation of proteins & nucleic acids by U.V method.

7. Estimation of turbidity using Nephlometer.

8. The separation of different macromolecules by Paper, Thin layer chromatography.

9. The separation of different macromolecules by Paper, PAGE, SDS-PAGE.

10. Estimation of minerals by Flame photometry.

11. Estimation of Thiamine and Riboflavin by Fluorimetry.

12. Preparation of Standard curve using UV-VIS & Flame Photometry

13. Fractionation of Plasma Proteins by Electrophoresis.

14. Biosensors.

Books:

1. Modern Experimental Biochemistry 3rd Edition - Boyer R, Pearson Education, (2000).

2. Instrumental Methods of Chemical Analysis (8th Edition) - Sharma B.K, Gel Publishing House, ((1999)).

cbit.ac.in - . | CHAITANYA BHARATHI INSTITUTE OF ...cbit.ac.in/files/B.TECH BIOTECH II YR II SEM.doc · Web viewAdvanced engineering Mathematics (Fifth edition) Wiley Eastern, Erwin

Documents