cbit.ac.incbit.ac.in/files/B.TECH BIOTECH IV YR I SEM_0.doc · Web viewPrinciples and Practice of Management –TN Chhabra & Dhanpat Rai and sons 6th edition New Delhi. (2000). 4.

BIOTECHNOLOGY SYLLABUS

SCHEME OF INSTRUCTION AND EXAMINATION

B.Tech. (Biotech) - IV YEAR - I Semester

SUBJECT CODE

SUBJECTS

PERIODS

/WEEK

MARKS

DURATION OF EXAM

Th /Pr

Sessionals

Exams.

Hrs

BTT.4.101

Tissue Engineering

4

30

70

3

BTT.4.102

Bioprocess Dynamics and Control

4

30

70

3

BTT.4.103

Environmental Biotechnology

4

30

70

3

BTT.4.104

Pharmaceutical Biotechnology

4

30

70

3

BTT.4.105

Principles and Practice of Management

4

30

70

3

BTT.4.106

Elective - II

4

30

70

3

BTP.4.107

Tissue Culture Lab

4

25

50

4

BTP.4.108

Bioprocess Lab

4

25

50

4

230

520

TISSUE ENGINEERING

Subject code : BTT.4.101

Sessional : 30

Periods / Week : 4

Examination : 70

Nature of Examination : Theory

Exam Duration : 3 Hrs

UNIT-I PLANT TISSUE CULTURE & PRODUCTIVITY PERFORMANCE

Plant Tissue Culture: Initiation and maintenance of callus and cell suspension culture, organogenesis. Biotic Stress resistance: Herbicide, Insect, Disease, virus etc., Abiotic stress tolerance, Drought, Temperature, Salt.

UNIT-II AGROBACTERIUM AND PLANT GENETIC ENGINEERING

Agro bacterium mediated gene transfer and cloning; Types of plant vectors and their use in genetic manipulation; and their application. Direct gene transfer methods; chemical methods, electroporation, microinjection, particle bombardment. Development of GM plants.

UNIT III MOLECULAR FARMING & INDUSTRIAL PRODUCTS

Application of Plant biotechnology for the production of quality oil, Industrial enzymes, Antigens (edible vaccine) and plant antibodies. Production of secondary metabolites from plant cell cultures; Process for production of secondary metabolites technology of plant cell culture for production of Chemicals. Bioreactor systems and models for mass cultivation of plant cells. Plant Vaccines.

UNIT IV: MAMMALIAN CELL CULTURE I

Basic techniques of mammalian cell culture in vitro; desegregation of tissue and primary culture, maintenance of cell culture; cell separation. Cell synchronization; Cell cloning and micromanipulation; Cell transformation; Application of animal cell culture; Scaling-up of animal cell culture.

UNIT V: MAMMALIAN CELL CULTURE APPLICATIONS

Stem cell cultures, embryonic stem cells and their applications; Cell culture based vaccines, Somatic cell genetics. Organ and histolytic cultures; Measurement of cell death; Apoptosis; Three dimensional culture and tissue engineering.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Plant Tissue Culture Theory and Practice: Bhojwani SS and Razdan Elsevier Science (2004).

2. Culture of Animal Cells by R. Ian Freshney, 3rd. edition). ... Mary G. Freshney, Eds, Culture of Immortalized Cells, Wiley-Liss, Inc.

3. Plant Tissue Culture By Vctor M. Loyola-Vargas2, C. De-la-Pea3, R. M. Galaz-valos2, F. R. Quiroz-Figueroa4, Springer, (2008).

4. Introduction to Plant Biotechnology by HS Chawla 4th edition Oxford and IBH publishers (2002).

5. Elements Of Biotechnology (11th Reprint ) by P.k. Gupta, Rastogi Publications.

REFERENCE BOOKS:

1. Plants, Genes and Crop biotechnology: Crispeels, MJ and Sadava DE Jones and Bartlett Publishers 2nd edition (2003).

2. Animal Cell Culture Methods - Vol 57: by Jennie P. Mather, David Barnes Academic Press; 1st edition (1998).

3. Biotechnology: Secondary Metabolites - Plants and Microbes by K.G. Ramawat, K.G. Ramawat; J.M. Merillon, Science Publishers,U.S. 2Rev Ed edition ( (2007).

4. Biotechnology and Genomics (2nd Reprint) by P.K. Gupta Rastogi Publications

Animal Biotechnology (Paperback - (2005) by A.K. Srivastava, R.K. Singh, M.P. Yadav, Oxford & IBH Publishing Co. Pvt. Ltd. (2005).

5. BIOPROCESS DYNAMICS AND CONTROL


Sessional : 30

Periods / Week : 4

Examination : 70



Unit I: Process Dynamics

Process variables, Load variables, Dynamics of simple processes Flow, level, Temperature, Pressure and Concentration; Transfer function Properties, response of simple processes for Step, Impulse and Sinusoidal Forcing functions.

Concept of Time Constant, Linearization, Response of first order systems in series - Non-interacting and Interacting systems.

Unit II: Control Actions and Controllers

Design of Control system measuring and final control elements, Open and Closed loop control, Negative and Positive feedback control, Servo and Regulatory problems.

Basic control actions Proportional, Integral and derivative actions P+I. P+D and P+I+D controls.

Reduction of physical system to block diagram, overall transfer functions for response of single capacity, two and three capacity systems, Problems encountered with pneumatic, hydraulic and electronic controllers to realize various control actions.

Unit III: Optimum Controller Settings

Controller Tuning Evaluation criteria with l/4th decay ratio, IAE. ISE, ITAE.

Tuning - process reaction curve method, Continuous oscillation method, damped oscillation method. Determination of optimum settings for mathematically described processes using time response and frequency response

Unit IV: Final Control Element

I/P Converter pneumatic, electric and hydraulic actuators. Control valves Construction, valve sizing, valve characteristics, valve positioner. Control of Globe, Butterfly and Diaphragm valves

Unit V: AdvAnced Control Strategies

Feed forward control, Ratio control, Cascade control, Split range multivariable control

Dynamics and Control of pH process and Biochemical reactor.


TEXT BOOKS:

1. Process control, Pollard A. Heinemann, Educational Books, London, 1971.

2. Process control, Harriott P., Tata McGraw-Hill publishing Co., New Delhi, Reprint 1991.

REFERENCE BOOKS:

1. Process Systems Analysis and Control, Donald R.Coughanowr, 2nd ed., McGraw Hill Inc., 1991.

2. Modern Control Engineering, Katsuhiko Ogata, 4th ed., Prentice-Hall of India Pvt. Ltd., New Delhi, (2003).

3. Process control, modeling, design and simulation, Wayne Bequette. B, Prentice-Hall of India Pvt. Ltd., New Delhi, (2004).

4. Principles of Process Control by Patranabis D, 2nd ed., Tata McGraw-Hill publishing Co., New Delhi, Reprint 1997.

5. Automatic process control, Eckman D.P., Wiley Eastern Ltd., New Delhi, (1993).

ENVIRONMENTAL BIOTECHNOLOGY


Sessional : 30

Periods / Week : 4

Examination : 70



UNIT I: BIOREMEDIATION

Introduction; Constraints and priorities of Bioremediation, Biostimulation of naturally occurring microbial activities; Bioaugmentation; Instiu, Exsitu, Intrinsic and Engineered Bioremediation; Solid phase bioremediation- Land farming, Prepared beds, Soil pipes, Phytoremediation, Bioventing and Biosparging; Liquid phase bioremediation.

UNIT II: METAL BIOTECHNOLOGY AND BIOFUELS

Mining and metal biotechnology; Microbial transformation; Accumulation and concentration of metals; Metal leaching; Extraction and future prospects.

Microorganisms and energy requirements of mankind; Role of carbon credits in Industries, present scenario around the world. Production of non-conventional fuels- Methane (Biogas); Hydrogen Alcohols and Algal Hydrocarbons. Use of Microorganism in augmentation of petroleum recovery.

UNIT III: BIOLOGICAL WASTE WATER TREATMENT

Biological processes for domestic and industrial waste water treatment; usage of algae and bacteria for waste water treatment. Aerobic systems Activated sludge process, trickling filters, Biological filters, Rotating biological contractors (RBC), Fluidized bed reactor (FBR), Expanded bed reactor, Inverse fluidized bed biofilm reactor (IFBBR), Packed bed reactors, Air sparged reactors; Anaerobic biological treatment-contact digesters, Packed column reactors, UASB.

UNIT- IV: DEGRADATION OF XENOBIOTIC COMPOUNDS

Introduction- Xenobiotic compounds; Recalcitrants- hazardous wastes- Biodegradation of Xenobiotics present in Environment- Decay behaviour and degradative plasmids; Hydrocarbons,Substituted Hydrocarbons, Oil Pollution and Bioremediation of Contaminated soils. Biological detoxification- Cyanide detoxification; Detoxification of oxalate, Urea, etc.; Toxic organics- Phenols.

UNIT- V: HAZARDOUS WASTE MANAGEMENT

Biotechnological applications to hazardous waste management; Examples of biotechnological applications to hazardous waste management; Global Environmental problems and Biotechnological approaches for management.


TEXTS BOOKS

1. General Microbiology Stanir RY, Ingraham JL, Wheelis ML & Painter RR, McMillan publications, 1989.

2. Environmental Biotechnology Foster CF, John Ware DA, Ellis Horwood Ltd. 1987.

REFERENCE BOOKS

1. Biotechnology and Biodegradation, Advances in Applied Biotechnology series Vol-4 Karnely D, Chakrabarthy, Omen GS, Gulf publications co., London, 1989.

2. Bioremediation Engineering: Design and application John T, Cookson Jr., McGraw Hill, Inc., (1985).

3. Environmental Biotechnology (2005) By Priv.-Doz. Dr.Hans-Joachim Jrdening, Prof. Dr. Josef Winter, Wiley-VCH Verlag GmbH & Co. KGaA.

4. Environmental Biotechnology: Principles and Applications by Bruce E.

5. Rittmann and Perry L. McCarty ( (2001)) McGraw-Hill 1st edition (2000).

6. Environmental Biotechnology: Theory and Application Gareth M. Evans, Judith C. Furlong ((2002)), Wiley .

7. Environmental Biotechnology ((2002)), by Geetha Bali Aph Publishing.

8. Environmental Biotechnology (2005)by A. H. Scragg (Author), Alan Scragg,

Oxford University Press.

PHARMACEUTICAL BIOTECHNOLOGY


Sessional : 30

Periods / Week : 4

Examination : 70



UNIT- I: FUNDAMENTALS OF PHARMACY PRACTICE

Pharmaceutical Biotechnology: An introduction, Origin & definition, Scope & Importance of Biotechnology; their applications, Microbes in Pharmaceutical industry, Methods of Gene transfer, Biotechnology production of Secondary Metabolites; Drug Interactions; Surgical supplies .

UNIT- II: DRUG METABOLISM AND PHARMACOKINETICS

ADME properties- Mechanism of Drug Absorption, Distribution of drugs, Drug metabolism (Biotransformation of drugs), Excretion of drugs, Pharmacokinetics: Basic considerations, Controlled Release Medication: Design of Controlled drug delivery systems, Drug release patterns, Oral, Parenteral, Transdermal, Ophthalmic, Intravaginal and Intrauterine Drug Delivery systems.

UNIT- III: PHARMACEUTICAL DOSAGE FORMULATIONS

Materials & Formulations, Manufacture of Tablets, Capsules, Sustained Release dosage forms, Parenteral solutions, Oral liquids, Emulsions, Ointments, Suppositories, Aerosols; Topical applications;

UNIT-IV: BLOOD AND PLASMA PRODUCTS

Collection, processing and storage of whole human blood, concentrated human RBC, dried human plasma, Human plasma protein fraction, Dried human serum, Human fibrinogen, Human thrombin, Human normal Immunoglobulin, Plasma substitutes- Ideal requirements, PVP, Dextran 40, control of Blood products, Transfusion products.

UNIT-V: PHARMACEUTICAL PRODUCTS

Fundamentals of Therapeutic categories such as Analgesics, Anesthetics, Antipyretic, Anti-inflammatory drugs, Antacids, Alkaloids, Glycosides, Hormone & Hormone antagonists; Anti-neoclassic and Immuno active drugs, Biologicals (Immunizing agents and allergenic extracts).


Text Books:

1. Biopharmaceutics & Pharmacokinetics A Treatise, by Brahmankar Dm, Sunil B Jaiswal, Vallabh Publications / Prakashan.(2006).

2. Pharmaceutical Biotechnology/S.S. Purohit, H.N. Kakrani and A.K. Saluja. Jodhpur, Agrobios, (2003), Student Edition.

3. Pharmaceutics Cooper and Guns, CBS publishers.1989.

Reference BOOKS:

1. The Theory and Practice of Industrial Pharmacy by Leon Lachman, Libermann (1996).

2. Remingtons The Science and practice of Pharmacy by Gennaro, A.R., Lippincot, Williams & Wilkins Publications, Philadelphia, (2002).

3. Pharmaceutics- The science of doses form design by Aulton, M.E., Churchill Livingstone publications London, (1996).

4. Text Book of Professional Pharmacy by NK Jain, CBS Publishers & Distributors. New Delhi. (2000).

5. Pharmaceutical Biotechnology- Daan J.A. Crommelin & Robert D. Sindelar & Bernd Meibohm, Informa Healthcare, (2007).

PRINCIPLES AND PRACTICE OF MANAGEMENT


Sessional : 30

Periods / Week : 4

Examination : 70



UNIT- I: FORMS OF BUSINESS ORGANIZATION AND MARKETING

MANAGEMENT

Salient features of Sole Proprietorship, Partnership, Joint Stock Company; Private Limited and Public Limited companies; Cooperative and Government owned companies; Merits and Demerits of above types. Functions of Marketing; Concepts of Selling and Marketing-Difference; Market Research; Product pricing; Distribution channels; Marketing mix (4 Ps); Advertising and sales promotion; Product life cycle.

UNIT-II: PRODUCTION, MATERIALS AND FINANCIAL MANAGEMENT -I

Forecasting-Moving average and exponential smoothing models; Functions of Production planning and control; Production systems-Types.

Inventory control-Relevant costs, Deterministic single item model with static demand, .j VABC, VED and FSN analysis; Introduction to MRP.

UNIT- III: PRODUCTION, MATERIALS AND FINANCIAL MANAGEMENT-II

Concept of time value of money; Interest formulae; Present and Future worth amounts for different cash flow patterns; Evaluation of alternative investment proposals (Capital r budgeting); Types of Capital-Fixed and Working capital; Working capital management-Factors and Principles; Depreciation- Straight line depreciation, Declining balance and Sum of Years digits methods.

UNIT- IV: ENTERPRENEUR DEVELOPMENT- I

Introduction, Entrepreneural characteristics, Functions of an Entrepreneur; Factors affecting entrepreneurship; Design of organization structure and business policy; Role of communication in entrepreneurship. Entrepreneurial development-Objectives, Need of Training for enterprises; Finance for the enterprises. Product, Process and Plant Design-Product analysis and Product Design process. Steps in process design and Plant Design.

UNIT- V: ENTERPRENEUR DEVELOPMENT- II

Project Report and Techno economic feasibility assessment; Project identification for palnning, project formulation; Project appraisal- Financial and environmental. Small scale industries- Role of SSI, Growth and Performance of SSIs in India. Reasons for sickness in SSIs; Steps to be taken for minimizing sickness in SSIs; Scope for self employment of Engineers.


SUGGESTED BOOKS:

1. Managerial economics and financial analysis by Arya sri 2nd Edition, Tata McGraw-Hill.

2. Marketing Management-Phillip Kotler.11th Edition. Prentice Hall.

3. Principles and Practice of Management TN Chhabra & Dhanpat Rai and sons 6th edition New Delhi. (2000).

4. Principles and Practice of Management- LM Prasad Chand Publications 6th Edition, New Delhi. (2001).

ELECTIVE - II

BIOPROCESS VALIDATIONS & CURRENT GOOD MANUFACTURING PRACTICES


Sessional : 30

Periods / Week : 4

Examination : 70



UNIT- I: BIOPROCESS VALIDATIONS

Validations- Prerequisites, Process Design & testing process characterization, Process Optimization, Validation Options, Prospective process validation, Retrospective validations, Concurrent validations, Revalidation, Organizing validation studies, Analytical methods validation, cleaning validation, pre-validation verification, Documentation, Control of cleaning materials & ancillary tools, frequency of cleaning, Development of validation protocol, Method of Evaluation.

UNIT- II: GOOD LABORATORY PRATICES (GLP)

Introduction to Good Laboratory Practices, Responsibilities in GLP, Quality assurance and facilities for GLP, Computational processes in GLP;

UNIT III: STANDARD OPERATING PROCEDURES (SOP)

Standard Operating Procedures (SOP) and Guidelines and regulations of PDA and ICH for GLP and GMP,

UNIT- IV: GOOD MANUFACTURING PRACTICES (GMP)

Introduction to GMP; Manufacturing & Quality control facilities; Sanitation & Hygiene; Control of raw materials, Packaging Materials, manufacturing processes, Minimization or Zero Contamination, and finished products; Documentation and compliance of GMP.

UNIT- V: GMP FOR BIOLOGICAL PRODUCTS

Products based on immunological principles, Layouts and Designs of Manufacturing Areas, Equipment designs and operations, Standard operating procedures for Production, Quality control, Labeling, Records and Waste Disposal; Health & hygiene of Persons involved.


Text Books:

1. How to Practice GMPs-PP Sharma.

2. Good Laboratory Practice: The Why and the How by Jurg P. Seiler, Springer-Verlag Berlin.

REFERENCE BOOKS:

1. Bioprocess Validation: The Present and Future by PhD Trevor Deeks, pub: PDA/DHI (2007).

2. Process Validation in Manufacturing of Biopharmaceuticals: Guidelines, Current Practices, and Industrial Case Studies (Biotechnology and Bioprocessing Series), Informa HealthCare; 2 edition (2005).

3. The L&K Process Guide, The tool for biopharmaceutical drug development, Pub: L&K Biosciences.

4. Bioprocess Engineering: Basic Concepts, 2/E Michael L. Shuler, Fikret Kargi, Dokuz ISBN-13: 9780130819086, Publisher: Prentice Hall (2002).

ELECTIVE- II

CANCER BIOLOGY


Sessional : 30

Periods / Week : 4

Examination : 70



UNIT- I: FUNDAMENTALS OF CANCER BIOLOGY

Cell cycle control, regulation of the cell cycle by cyclins, cyclin-dependent kinases, cdk inhibitors, and tumor suppressors, Mutations that cause changes in signal molecules, Effects on receptor, Signal switches, Tumor suppressor genes, Modulation of cell cycle in cancer, Different forms of cancer, Diet and cancer.

UNIT- II: PRINCIPLE S OF CARCINOGENESIS

Chemical Carcinogenesis, Metabolism of Carcinogenesis, Natural History of Carcinogenesis, Targets of Chemical Carcinogenesis, Principles of Physical Carcinogenesis, X-ray radiation- mechanism of Carcinogenesis.

UNIT- III: PRINCIPLES OF MOLECULAR CELL BIOLOGY OF CANCER

Oncogenes, Identification of Oncogenes, Retroviruses and Oncogenes, Detection of Oncogenes, Growth factor and Growth factor receptors that are Oncogenes. Oncogenes / Proto Oncogene activity. Growth factors related to transformations.

UNIT- IV: CANCER METASTASIS AND TREATEMENT

Clinical significance of invasion, Heterogeneity of metastatic phenotype, Metastatic cascade, Basement Membrane disruption, Three-step theory of invasion, Proteinases and tumor cell invasion.

Different forms of therapy- Chemotherapy, Radiation therapy. Detection of cancers, Prediction of aggressiveness of Cancer, Advances in Cancer detection.

UNIT- V: Principles of Cancer Pharmacology:

Oncogenes and cell nuclear receptors and cancer, etiology of cancer, pharmacokinetics and pharmacodynamics of anticancer drugs. Metabolism of anticancer drugs, inter individual differences in response to anticancer drugs, antisense inhibition of gene expression, mechanisms of anticancer drug resistance. Molecular targets for drug development, and chemoprevention.


Text Books:

1. Virology-A practical approach- Maly B.W.J., IRL Press, Oxford, 1987.

2. Introduction to Modern Virology- Dunmock NJ and Primrose.S.B., Blackwell scientific Publications. Oxford, 1988.

3. An Introduction to Cellular and Molecular Biology of Cancer- Oxford Medical Publications, 1991.

Reference BOOKS:

1. Introduction to biology of Cancer by Franks and Taich Oxford Publishers.

2. Cancer Biology by Raymond W. Ruddon, Oxford University Press, USA; 3rd edition (1995).

3. The Biology of Cancer by Robert A. Weinberg, June (2006), Garland Science.

4. Molecular Biology of Cancer 2nd Edition by Fiona Macdonald, Christopher Ford, Alan Casson (2004) | Pub: Taylor & Francis.

5. Principles Of Cancer Biology (2006), by Lewis Kleinsmith, Publisher: Benjamin- Cummings Publishing Company.

6. Introduction to Cancer Biology by Momna Hejmadi Pub: Bookboom.

7. Cancer Biology: By King, Roger J. B., Robins, Mike W. Publisher: Prentice Hall.

ELECTIVE- II

BIOPHYSICS OF MACROMOLECULES


Sessional : 30

Periods / Week : 4

Examination : 70



UNIT- I: INTRODUCTION TO BIOPHYSICS AND CONFORMATIONAL ANALYSIS

Levels of structures in Biological Macromolecules; Central questions of Biophysics;

Basic strategies in Biophysics. Forces that determine protein and nucleic acid structure; Basic Problems; Polypeptide chains, Geometries; Potential energy calculations; Observed values for rotation angles; Hydrogen Bonding; Hydrophobic interactions and water structures; Ionic interactions, Disulphide bonds.

UNIT- II: STRUCTURAL ANALYSIS OF MACROMOLECULES

Prediction of protein structure; Nucleic acids- general characteristics of nucleic acid structure; Geometries, Glycosidic bond rotational isomers, backbone rotational isomers and ribose puckering forces stabilizing ordered forms; Base pairing, Base stacking; Tertiary structure of nucleic acids.

UNIT- II: KINETICS OF LIGNAD INTERACTIONS

Biochemical Kinetic studies; Uni-molecular reactions, Simple bimolecular multiple

intermediates; Steady state kinetics, Catalytic efficiency relaxation pectrometry,

Ribonuclease as an example.

UNIT- IV: TECHNIQUES FOR THE STUDY OF BIOLOGICAL

STRUCTURE & FUNCTION

Size and shape of micro molecules; Methods of direct visualization; Macromolecules as hydrodynamic particles; Macromolecular diffusion; Ultra centrifugation; Viscometry; X-ray crystallography, Determination of Molecular structures; X-ray diffraction, Electron Microscopy; Neutron scattering; Light scattering.

UNIT V: STRUCTURAL ANALYSIS OF MACROMOLECULES

Prediction of protein structure; Sequence-structure relationships, Nucleic acids; general characteristics of nucleic acid structure, geometric, glycosidic bond rotational isomers backbone rotational isomers and ribose puckering forces stabilizing ordered forms, base pairing, base stacking; tertiary structure of nucleic acids.


Text Books

1. Biophysical Chemistry- Cantor R, Samuel PR, WH Freeman & Co, (1985).

2. Methods in Molecular Biophysics: Structure, Dynamics, and Function by: Igor N. Serdyuk, Nathan R. Zaccai, and Joseph Zaccai Cambridge University Press.

3. Applied Biophysics, A Molecular Approach for Physical Scientistsby Waigh, Tom1st Edition - John Wiley & Sons, (2007).


Sessional : 25

Periods / Week : 4

Examination : 50



ELECTIVE- II

GENOMICS AND PROTEOMICS

UNIT- I: PROKARYOTE GENOME ORGANIZATION & EXPRESSION

Genome organization- Prokaryotic genome, Prokaryotic gene structure-, Regulation of Gene Expression- Promoters; Enhancers; Operon concept (Lac Operon, Trp Operon, Attenuation) Types of Operon regulations.

UNIT- II: EUKARYOTIC GENE STRUCTURE AND REGULATION OF GENE EXPRESSION

Eukaryotic Genome organization, Gene structure and Regulation of Gene Expression: Transcriptional level, processing level and translational level. Repetitive DNA, Junk DNA. Post-Transcriptional and Translational Modifications.

UNIT- III: TRANSPOSABLE ELEMENTS

Transposable elements: Transposons, Insertion sequences, Eukaryotic Transposable elements, Implication of Transposable elements in the genome, Genome variation, Information maintenance (DNA damage and repair), Protein structure, Protein-Protein interaction- Methods of determination.

UNIT- IV: GENETIC SEQUENCING, MAPPING OF GENOME & GENE PREDICTION

Sequencing DNA, Genome Projects of Microbes and Invertebrates- Plant, Human and Animal; Model organisms; Gene prediction- Intrinsic, Comparing Genomes; Homology based gene prediction, Biological Methods of studying Gene Expression- EST approach in Biology, Micro arrays- Basics; Application of cDNA arrays, Genomics and Genome variations, Proteomics- Basics, Proteomics-Applications.

UNIT- V: METABOLOMICS

Metabolomics Basics. Pharmacogeormcs, Single Nucleotide Polymorphism; Disease Genes and their identification. Drug uptake, action and metabolism. Drug targets and Designer medicine, Genomics perspective of Bioterrorism, Ethical and Legal implications, Intellectual property rights.


Text Books

1. Genomics and Proteomics-Functional and Computational Aspects- S Sahai, Plenum Publications, (1999).

2. Bioinformatics-Methods and Application, Genomics, Proteomics, and drug discovery- by S.C.Rastogi, N. Mendiratta, P. Rastogi. 2nd edition. Prentice Hall of India, New Delhi.

Reference BOOKS:

1. Cell and Molecular Biology: Concepts and Experiments, John Wiley and Sons Ltd. (1996).

2. Genetics: Analysis of Genes and Genomes, Daniel L. Hartl, Elizabeth W. Jones, (2001), Jones & Bartlett Pub.

3. Discovering Genomics, Proteomics, and Bioinformatics by A. Malcolm Campbell, Laurie J. Heyer, Pub:Benjamin Cummings, (2002).

4. Fundamentals of Data Mining in Genomics and Proteomics Dubitzky, Werner; Granzow, Martin; Berrar, Daniel P. (2007), Springer.

5. Genomics and Proteomics: Functional and Computational Aspects (Hardcover)

by Sndor Suhai, Springer 1st edition, (2000).

6. Genetics and Genome Science; Biotechnology; Molecular Biology; Proteins and Proteomics By A. Malcolm Campbell, Laurie J. Heyer, Benjamin Cummings Pearson Education (1996).

TISSUE CULTURE LAB

Subject code : BTP.4.107

Sessional : 25

Periods / Week : 4

Examination : 50



LIST OF EXPERIMENTS

1. Preparation of Media

2. Surface sterilization

3. Callus induction

4. Organ culture

5. Micro propagation of horticultural/medicinally important plants

6. Protoplast isolation, culture and Cytological examination

7. Agrobacterium mediated gene transfer

a) Hairy roots

b) Selection of transformants: reporter gene (GUS) assays.

8. Animals Cell Culture/primary cell cultures

BIOPROCESS LABORATORY

Subject code : BTP.4.108

Sessional : 25

Periods / Week : 4

Examination : 50



LIST OF EXPERIMENTS

1. Sterilization techniques and thermal death kinetics

2. Medium optimization: Plackett-Burman design.

3. Studies on batch fermentation.

4. Estimation of kinetic parameters in Chemostat.

5. Kinetic studies on fed-batch fermentation.

6. Estimation of residence time distributions.

7. Determination of volumetric mass transfer coefficient by dynamic gassing out technique.

8. Determination of volumetric mass transfer coefficient by sulphate oxidation technique.

9. Study of the rheological parameters in fermentation broths.

10. Determination of power number.

11. Estimation of Bio-Products. (Organic acids, alcohols)

12. Studies on immobilized enzymes/cells in packed bed reactor.

13. Studies on immobilized enzymes/cells in fluidized bed reactor.

14. Kinetic studies in air lift fermentor.

15. Design exercises on fermentors.

cbit.ac.incbit.ac.in/files/B.TECH BIOTECH IV YR I SEM_0.doc · Web viewPrinciples and Practice of Management –TN Chhabra & Dhanpat Rai and sons 6th edition New Delhi. (2000). 4.

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