Applied StemCell Applied Stem Cell Inc.’s proprietary TARGATT™ technology enables highly efficient site-specific gene integration in mammalian cells and animals 1, 2 . This technology uses ФC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT™ technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ФC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT™ technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models. Fast & Site-Specific Knock-in Technology Fast & Site-Specific Knock-in Technology Gene of interest (eg. GFP) TARGATT™ Vector with Screening pups for site-specific gene integration Transfection Embryos with attp sites are collected from TARGATT™ mouse. References: 1. B. Tasic et al., Proc Natl Acad Sci USA. 108, 7902 (2011) . 2. J. Rossant, L. M. J. Nutter, M. Gertsenstein, Proc Natl Acad Sci USA. 108, 7659 Background: Applications Applications How it works: TARGATT™ attB 1 2 in vivo screenings Generation of humanized models Generation of disease models Generation of drug / genome interaction models in vitro screenings Drug discovery Study drug interactions Toxicity Study Personalized medicine 4 Toll Free Phone: 1-866-497-4180 www.appliedstemcell.com Applied StemCell Toll Free Phone: 1-866-497-4180 www.appliedstemcell.com 1 2 TARGATT™ Embryos & Transgenic Kit Fast & Site-Specific Knock-in Mouse Service TARGATT™ Services and Products If you prefer to generate a knock-in mouse model on your own, TARGATT™ Embryo and Transgenic Kits are available for purchase. For your convenience, you may also mix and match any of our TARGATT™ products and services. TARGATT™ H11 Embryos FVB (Frozen) AST-0002 $3,600* TARGATT™ H11 Embryos C57BL/6 (Frozen) AST-0012 $4,800* TARGATT™ Rosa26 Embryos FVB (Frozen) AST-0004 $3,600* TARGATT™ Transgenic Kit AST-1002 2 rounds of microinjections $750 MTA is required upon ordering. *Quotes for academic research. Please contact us for industrial rates. Traditional Knock-in Comparison of timelines for Knock-in Mouse Models TARGATT™ System Vector Design Pronuclear Microinjection F0 Genotyping 3 months Vector Design ES cell Targeting Chimeras Germline Mice Blastocyst microinjection Technology Overview vs 10 months Service Includes: Vector design and construction Integration enzyme generation DNA preparation Embryo microinjection / Implantation Animal care-housing Genotyping Knock-in Mouse Service (C57BL/6 strain) AST-5021 $19,000 Knock-in Mouse Service (FVB strain) AST-5001 $17,000 Features: Fast: Our proprietary technology will generate your site-specific knock-in mouse in 3 months. Reliable: Any gene of interest can be specifically inserted at a defined, transcriptionally active locus. We guarantee transgene expression. Versatile: Tissue specific and/or ubiquitous expression are available. Reliable Gene Expression Efficiency Up to 40% Low Site-Specificity Yes Yes Transgene Expression Yes Yes Transgene Copy Number One copy One copy Turnaround Time 3 Months 10 Months TARGATT™ System Traditional Rosa26 Knock-in Generate master TARGATT™ Cells with attp sites (6 months) TARGATT™ Cell Lines Expressing Gene of Interest Knock-in Mouse Generation in vivo Model in vitro Model Stable Cell Line Generation 1 2 3 4 3 Pronuclear injection into TARGATT™ embryos. within 3 months Knock-in Mouse within 1 - 3 months Knock-in Cell Line
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Applied StemCell
Applied Stem Cell Inc.’s proprietary TARGATT™ technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses ФC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT™ technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ФC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.TARGATT™ technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.
Fast & Site-Specific Knock-in TechnologyFast & Site-Specific Knock-in Technology
Gene of interest (eg. GFP) TARGATT™ Vector with
Screening pups for site-specific gene integration
Transfection
Embryos with attp sites are collected from TARGATT™ mouse.
References:1. B. Tasic et al., Proc Natl Acad Sci USA. 108, 7902 (2011) .2. J. Rossant, L. M. J. Nutter, M. Gertsenstein, Proc Natl Acad Sci USA. 108, 7659
Background:
Applications Applications
How it works:
TARGATT™
attB
1
2
in vivo screenings Generation of humanized models Generation of disease modelsGeneration of drug / genome interaction models
in vitro screenings Drug discoveryStudy drug interactionsToxicity StudyPersonalized medicine
If you prefer to generate a knock-in mouse model on your own , TARGATT™ Embryo and Transgenic Kits are available for purchase.For your convenience, you may also mix and match any of our TARGATT™ products and services.
MTA is required upon ordering. *Quotes for academic research. Please contact us for industrial rates.
Traditional Knock-in
Comparison of timelines for Knock-in Mouse Models
TARGATT™ System
Vector Design
Pronuclear Microinjection
F0 Genotyping
3 months
Vector Design
ES cellTargeting
Chimeras
Germline Mice
Blastocyst microinjection
Technology Overview
vs
10 months
Service Includes:Vector design and constructionIntegration enzyme generationDNA preparation Embryo microinjection / ImplantationAnimal care-housingGenotyping
Knock-in Mouse Service (C57BL/6 strain) AST-5021 $19,000 Knock-in Mouse Service (FVB strain) AST-5001 $17,000
Features:Fast: Our proprietary technology will generate your site-specific knock-in mouse in 3 months.Reliable: Any gene of interest can be specifically inserted at a defined, transcriptionally active locus. We guarantee transgene expression.Versatile: Tissue specific and/or ubiquitous expression are available.
Reliable Gene Expression
Efficiency Up to 40% Low Site-Specificity Yes Yes Transgene Expression Yes YesTransgene Copy Number One copy One copyTurnaround Time 3 Months 10 Months
TARGATT™ System
Traditional Rosa26 Knock-in
Generate master TARGATT™ Cells with attp sites (6 months)
Based on our proprietary site-specific TARGATT™ technology for fast knock-in (KI) mouse generation, Applied StemCell (ASC) recently developed a TARGATT™ system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.
Adding TARGATT™ sites to your cell line allows you to insert any gene of interest in a specific locus in the genome.
Site-Specific
Knockin efficiency
Transgene copy
Off-target event
Others
Yes
Very low
Single
Very Low
Yes
High
Single
Very Low
Yes
Low
Single
Yes
No
High
Multiple
Yes
No
High
Multiple
Yes
Technical Advantages of TARGATT™:
Application of the TARGATT™ System for the Generation of Site-Specific Knock-in Mammalian Cell Lines
ASC provides high quality, one stop services for the generation of gene targeted mouse models. Cost-effective: Applied StemCell, Inc. has years of experience and a high success rate in generating custom gene targeted mice using our proprietary germline transmittable mESC lines. High Quality: We have high QC standard checkpoints at every step to ensure your project progresses smoothly.Fast Turnaround Time: We streamline every step. Gene targeted mouse models can be generated in as fast as 7 months.
Conventional Knock-in/out Mouse Generation Service
DNA Cloning Services
Virus Packaging Service
Behavioral Phenotyping Service for Knock-in/out MiceCompare behavioral differences between transgenic mice & wild type mice.
We provide high titer to ultra-high titer, ready to use VSV-G pseudoviral packaging services.
Service Includes:Gene targeting vector constructionES cell targeting Cre/FLP recombination Chimera production via blastocyst injectionChimera breeding to germline Mouse genotyping
Chimera from blastocyst injection
Examples of services:Cloning of amplified DNA fragmentsGene targeting vector construction for homologous recombination Bacterial Artificial Chromosome (BAC) recombineeringGeneration of RNAi and inducible vectorsSite-specific mutagenesisDe novo gene synthesis
Other Mouse Models / Gene Targeting Services
Custom Knock-in Cell Line Service AST-6021 Inquire TARGATT™ Knock-in Cell Line Service AST-6001 $25,000
Mouse ESC Derivation Service ASC-6001 $2,900 Our scientists have successfully derived over 80 new mouse embryonic stem cell l ines, including those from gene targeted embryos.
To publish your new ES or iPS cell line, do you need:- Stringent molecular and functional assays to evaluate pluripotency criteria?- Karyotype analysis to rule out genetic aberrations?
We offer all services needed for a complete characterization of your pluripotent cell lines.
Teratoma Formation Analysis ServiceFeatures:- Turnaround time: 4- 12 weeks- Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6 Human ESCs/iPSCs = 1 x 10^6 - Success Rate: >97%
Service Highlights:Injection Specifics: A total of 4 mice (3 experimental and 1 control) will be used. Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.Steps: Cell injection, teratoma harvesting, Tissue Sectioning, H&E staining and histology analysis of teratoma sections.
Service Includes:1. Complete report with histological analysis and high quality/publication grade images of endoderm, mesoderm and ectoderm formation. 2. Tissue blocks and H&E stained tissue section slides
References:K. Chen et al., Stem Cell Research USA. 9, 237–248 (2012)We are the exclusive teratoma service provider for Coriell Institute (Patient Specific iPSCs). See our service reports here:http://ccr.coriell.org/Sections/Collections/NIGMS/ipsc_list.aspx?PgId=696&coll=GM
FAQQ: What is your success rate for teratoma formation?A: After analyzing control and test samples, we have determined that 90% of our injections give rise to verifiable teratomas. Injections into both the kidney capsule and the testis ensures teratoma formation from pluripotent cells in one or both organs. The site and degree of teratoma formation is cell line dependent. For example, only 82% of hIPSC form teratomas in both the kidney and the testis. Other cell lines form teratomas in only the kidney or testis. By injecting three mice at two sites per test sample, we ensure that the best conditions are used to observe teratoma formation.
Q: How long does it take to get results?A: To form mature teratomas, it takes 3-6 weeks for mouse ES/iPSCs and 5-10 weeks for hES/iPSCs. As a general practice, we allow the teratomas to grow for up to 12 weeks before we sacrifice the mice.
Q: How do I prepare cells for the assay?A: Follow the instructions outlined in the Requisition Form found at http://www.appliedstemcell.com/userfiles/files/Teratoma_Service_Requisition_Form.pdfTypically, two T75 flasks are sufficient for the assay. We recommend that the cells be shipped (by one day delivery) at 70% confluence in flasks filled completely with culture media.
Enhanced differentiation capability:Our technique of injection under both the kidney capsule and testis results in distinct teratoma formations with differentiated tissues derived from all three germ layers (A to F).
High Teratoma Formation Rate:Three recipient mice per sample are dually injected under BOTH the kidney capsule and testis to ensure teratoma formation from pluripotent cells at one or both organs. ~82% of human iPS cell lines form teratomas in both the kidney and testis. 8.9% forms kidney tumor only, 2.6% forms testis tumor only.
Technique Highlights
Kidney
+ + 82.3 %
+ - 8.9 %- + 2.6 %
- - 6.2 %
Testis% of iPSC Lines Producing
Publication Grade Teratomas*
*Teratomas with distinct tissue types
Satisfaction Guaranteed!!Please contact us with the information
about your samples for details and pricing
A
D E F
G H I
B C
Embryoid Body (EB) Formation and Characterization Service
Features:- A complete service that includes embryoid body (EB) formation and characterization to assess the pluripotency of your ESC/iPSC lines.- A fast alternative to traditional teratoma formation methods. Our turnaround time is approximately 3 weeks.- Ideal for rapid initial screenings or large sample screenings.
EB Formation and Characterization Service ASC-9020 $980/sample (Additional $500 culture fee for frozen samples may apply)
A
B
I II
I. EB derived from GFP-expressing human ESCs.
II. β- Tubulin III (ecdoderm) SMA: Smooth muscle actin (mesoderm) AFP: Alpha fetal protein (endoderm)
β-Tubulin III SMA
AFP
Satisfaction Guaranteed!!Contact us with information
about your samples for details and pricing
Service Includes:- Formation of EBs.- IHC staining of EBs for pluripotency markers (Alpha fetoprotein for endoderm, smooth muscle actin for mesodern and Beta III tubulin for ectoderm).- A full report that includes high resolution, publication-grade images.
8
A : Cartilage B : Neuronal rosetteC : GlandsD : Pigmentaed cellsE : Bone F : Stratified squamous epithelium G - I : Subcutaceous injections give rise to poorly differentiation tumor tissue.
Genome Footprint-Free iPS Cell Generation Service (mRNA/Episomal)Frustrated with the amount of time and effort required to generate and characterize iPS cell lines? Wishing your valuable time was directed towards addressing the high priority issues of your research project rather than the time consuming tasks of iPS cell line generation and characterization? Let Applied StemCell generate the iPS cell line for you.
Features:- Our non-integrating method ensures the absence of a genomic footprint: ideal for drug discovery and cell therapy applications.- Two methods are available: DNA episomal vectors containing the reprogramming factors or synthetic mRNA of each reprogramming factor.- A high reprogramming efficiency is achieved with both the mRNA (0.5%) and the episomal vectors (0.2%).- Both reprogramming vectors and mRNA are removed during the cell cycle. - Safety: both episomal vectors and mRNA are safe to handle.
Further Characterization Services Available:- Karyotyping- Embryoid Body (EB) Formation and Characterization to test iPSC pluripotency- Teratoma Formation Analysis Service to test iPSC pluripotency
iPSC Generation Service (mRNA) ASC-6022 $10,000 iPSC Generation Service (Episomal) ASC-6023 $10,000
Cell Recovery and QuarantinePrimary Cell Line
(i.e. skin fibroblasts)
ReprogrammingmRNA
or
Colony Formation
Colony Expansion
Cryopreservation
Transfection with Episomal DNA Vectors
or mRNA
Characterization viaantibody staining and
RT-PCRTRA-1-60
Oct-4
Reprogramming Plasmids
6-Step iPSC Generation Service can generate iPS cell lines using two proven genome footprint-free methods (mRNA or episomal vectors)
hESC/iPSC Neuronal Differentiaton Service Do you want to differentiate your pluripotent cell line into neurons or glial cells?Let us make your life easier! We will make Neural Stem Cells (NSCs) or Neural Crest Stem Cells (NCSCs) for you! -You send us your pluripotent cell line (or we can make one for you)-We use our proprietary neural induction protocol and reagents to differentiate your ESC/iPSC into neural stem cells (NSCs) or neural crest stem cells (NCSCs)-NSCs and NCSCs are proliferating cells that can be frozen and cultured over a prolonged period of time. They are also capable of further differentiation into multiple neural cell types.
NSCs Differentiation Service ASC-8001 $9,500 NCSCs Differentiation Service ASC-8001C $9,500
Related Services:Cardiomyocytes Differentiation Service ASC-8003 $5,000Skeltal Muscle Differentiation Service ASC-8005 $5,000
References: *K. Takahashi et al., Cell. 131, 861-72 (2007)
ASC’s iPS Cell Generation Kit contains an optimized ratio of retroviral reprogramming vectors that has been modified for the consistent generation of iPS cells*. A user-friendly protocol along with our excellent customer service will make the task of iPS cell generation worry-free.
Individual retrovirus is also available:Human iPSC VSVg Retrovirus Cocktail 0.4 mL ASR-2005 $1,000High Titer GFP VSVg Retrovirus 0.1 mL ASR-2006 $230
iPS Cell Generation Kit (Retrovirus)
Virus
GFP
hOCT4
hSOX2
DAPI Merge
Top row: BJ cells (Human Fibroblasts) were transduced for 24h with Retro-GFP (MOI: 0.2).
Kit Includes:1. A ready-to-use cocktail of retroviral reprogramming factors (Oct4, Sox2, Klf4 and LMyc)The concentration of each vector has been optimized to obtain a high reprogramming efficiency.2. Retro-GFP This GFP-containing vector is used for the determination of the optimal condition to maximize the transduction efficiency. 3. Polybrene To enhance efficiency of transduction.4. Complete medium Just mix the medium supplements and Basal hiPS cell medium included in the kit. 5. Feeder cells CF-1 MEFs are provided for candidate clone culturing
Features:High Reprogramming Efficiency User-Friendly ProtocolEvery batch of retrovirus is tested for its ability to generate iPSC colonies
ESC/iPSC Characterization Kit
ES/iPS Cell Pluripotency RT-PCR Kit
Features:Sophisticated Marker Selection: Each kit includes an optimal panel of pluripotency markers.
High Sensitivity: Partially differentiated hiPSC clones can be detected.
Convenient: The ready-to-use kit contains all the buffers and solutions needed to perform immunocytochemical and enzyme activity analyses.
Looking for an alternative to expensive protein neural-induction reagents?Tired of the heterogeneous nature of embryoid body formation and the subsequent protracted length of neuronal differentiation?ASC’s Neural Stem Cell Generation Kit is your answer!
Neuro-Sure™ Medium: Number of Rosette Clusters / Well
Features:- Fast, efficient, and user-friendly- Special formula: Increases the efficiency of rosette formation by 10-fold over standard neuron and/or induction media- Generation of proliferating Neural Stem Cells (NSCs) that can give rise to large quantities of neuronal and glial cells- Suitable for the generation of various neural cells (glial cells, astrocytes, dopaminergic neurons, motor neurons)
ASK-4002 Neural Stem Cell Generation & Characterization Kit $700ASK-4003 Neural Stem Cell Generation Kit $500ASK-4001 Neuro-Sure™ Induction Medium Size: 100 mL $150ASK-4101 Neuro-Sure™ Culture Medium Size: 100 mL Inquire
Neuro-Sure™ (ASM-4001)Standard NIM
3 30
a) A single Rosette cluster is obeserved using standard neural induction medium.b) Multiple rosette clusters are observed using Neuro-Sure™ Medium.
Mouse Embryonic Fibroblast (MEF) cells are used as feeder layers for ES/iPS cell cultures. We have a broad selection of high-quality MEF products including:
CF-1 Neomycin-resistant DR4 (resistant to neomycin, hygromycin, puromycin, and 6- thioguanine)
DR4, P2, untreated ASF-1001 1 x 10^6 cells $180 DR4, P3, irradiated ASF-1013 4 x 10^6 cells $120 ASF-1015 2 x 10^6 cells $70
CF-1, P2, untreated ASF-1201 1 x 10^6 cells $90 CF-1, P3, irradiated ASF-1213 4 x 10^6 cells $45 ASF-1215 2 x 10^6 cells $35
CF-1, P3, mitomycin-C treated ASF-1223 4 x 10^6 cells $45 ASF-1225 2 x 10^6 cells $35
Neo-resistant, P2, untreated ASF-1101 1 x 10^6 cells $90 Neo-resistant, P3, irradiated ASF-1113 4 x 10^6 cells $45 ASF-1115 2 x 10^6 cells $35
SNLSNL 76/7 feeders, STO cell line, P12, untreated ASF-1305 2 x 10^6 cells $90 SNL 76/7 feeders, STO cell line, P14, irradiated ASF-1317 5 x 10^6 cells $45
SNL 76/71, 2,3, derived from the mouse fibroblast line STO, is transformed with both the neomycin and LIF genes and has been shown to support the growth of both ES and iPS cell lines.
References:1. Okita, K; Ichisaka, T; Yamanaka, S. Nature 448:313–317. (2007) 2.Takahashi K, Okita K, Nakagawa M, Yamanaka S. Nat Protoc. 2:3081-9. (2007) 3. Takahashi K, Narita M, Yokura M, Ichisaka T, Yamanaka S. PLoS One 4(12):e8067 (2009)
MEF Feeder Cells (Mouse Embryonic Fibroblasts)
- Average viability >95%- Tested for long term culture of either mouse or human ES/iPSC lines- Manufactured at our US facility- Rapid inventory turnover ensures fresh stocks are shipped within 2-3 days- Our MEFs have been used by more than 200 clients in both academic and industry labs.
MEF Conditioned Medium ASC’s Conditioned Medium is harvested from cultured CF-1 MEFs. Our stringent quality control and functional control assays ensure the pluripotency of your ES/iPSC lines will be maintened in this feeder-free culture system.ASM-5008 100 ml $120
Serum- and Feeder-Free Medium (SFFM)ESC-Sure™ SFFM is an optimized medium formulated for the culture of human ES/iPSC lines requiring serum-free and feeder-free culture conditioins. ASM-5010 100 ml $99
Mouse ESC Culture Supplement ESC-Sure™ mESC Mate is a basal medium supplement that contains all the factors required by mESC lines.ASM-5011 100 ml $200
Mouse ESC Complete Medium ESC-Sure™ mESC Complete Medium is a ready-to-use medium that contains all the reagents required for mouse ESC and iPSC cultures. ASM-5022 500ml $180
Neural Stem Cell Differentiation MediumNeuro-Sure Differentiation Medium is a specially formulated medium designed to increase the efficiency of the differentiation of ESCs or iPSCs into neural epithelia. The increase in differentiation efficiency is 10-fold higher than those resulting from standard neural induction media formulas. ASM-4011 100ml $120
ESC/iPSC Culture Media
Stem Cell Growth / Differentiation Factors Wnt PathwayASG-2003-1 DKK-1 (Human Cells) 25 ug $240ASG-2005-1 Frizzled-5 Fc Fusion (Human Cells) 50 ug $210ASG-2006-1 Frizzled-8 Fc Fusion (Human Cells) 50 ug $210ASG-2011-1 SFRP5 (E.Coli) 50 ug $330ASG-2014-1 Wnt-3a (Human Cells) 5 ug $280ASG-2015-1 Wnt-5a (Human Cells) 5 ug $380
Hedgehog PathwayASG-2012-1 Sonic Hedgehog (Human cells) 5 ug $260
TGF-beta Family PathwayASG-2001-1 Activin A (Human Cells) 5 ug $180ASG-2002-1 BMP-4 (Human Cells) 5 ug $195 ASG-1012-1 BMP-7 (Human Cells) 10 ug $213ASG-2010-1 Noggin (Human Cells) 50 ug $215ASG-2013-1 TGF-beta1 (Human Cells) 5 ug $199
OthersASG-0003 bFGF (E.Coli) 50 ug $95ASG-1010-1 beta-NGF (Human Cells) 10 ug $104ASG-2004-1 EGF (E .Coli) 50 ug $85ASG-1014-1 EPO (Human Cells) 10 ug $240ASG-1004-3 GM-CSF (Human Cells) 10 ug $213 ASG-1006-3 IFN-alpha 2A (Human Cells) 10 ug $142ASG-2007-1 IGF-I Long R3 (E. Coli) 100 ug $120ASG-2008-1 Insulin (E. Coli) 1000 ug $110ASG-1005-2 IL-2 (Human Cells 10 ug $109ASG-1007-2 M-CSF (Human Cells) 10 ug $207ASG-1009-2 TNF-alpha (Human Cells) 10 ug $87
Request samples!We provide free samples of bFGF, Wnt-3a, TGF-beta1, Noggin, and BMP-4.Contact us at [email protected]. Promo # ASCRP08
GermLine* C57BL/6-EZ ASE-9007 2.5 x 10^6 cells $900 GermLine* C57BL/129SvJ ASE-9005 1 x 10^6 cells $795 GermLine* 129-EZ ASE-9006 2.5 x 10^6 cells $795 GermLine* mESC BALB/c EZ ASE-9008 2.5 x 10^6 cells $600 mESC C57BL/6-ALBINO ASE-9009 1 x 10^6 cells $795
*Germline transmission guaranteed
Human iPS Cell Lines generated by retrovirus vectors or episomal expression.
ESC-Sure™ Fetal Bovine Serum (FBS) is the only serum product on the market that is tested for germline transmission of mouse ES cells.
Germline transmission tested! Mycoplasma-free Ready-to-use (no heat inactivation needed) Virus-free Toxin-free Competitively priced Each lot of ESC-Sure™ FBS is put through a series of quality control tests in addition to a series of functional tests. These tests ensure that each lot of ESC-Sure™ supports the normal morphology, karyotype, pluripotency, and germline transmission of mouse ESCs. Moreover, the series of quality control tests are conducted over several freeze/thaw cycles, therefore ensuring that every lot of ESC-Sure™ purchased is of the highest quality.
Mouse ES cells cultured in ESC-Sure™ FBS maintain normal morphology and pluripotency. ES cells cultured in our FBS are competent for tetraploid complementation.
Germline transmission test by tetraploid complementation